Vasiliy S. Chernyshev

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of the Russian Federation

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Co-Authors

Dmitry A. Gorin

Skolkovo Institute of Science and Technology

Mikhail Skliar

University of Utah

Alexey M. Yashchenok

Skolkovo Institute of Science and Technology

Roman N. Chuprov‐Netochin

Moscow Institute of Physics and Technology

A. Yu. Kuzin

Skolkovo Institute of Science and Technology

Alexander Golikov

Moscow State Pedagogical University

Gregory Goltsman

Moscow State Pedagogical University

Polina G. Rudakovskaya

Skolkovo Institute of Science and Technology

Vadim Kovalyuk

National Research University Higher School of Economics

P An

Russian Quantum Center

Cooperative Institutions

Skolkovo Institute of Science and Technology

Lomonosov Moscow State University

Russian Academy of Sciences

Moscow Institute of Physics and Technology

Moscow State University

Saratov State University

University of Utah

National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of the Russian Federation

Moscow Power Engineering Institute

Institute of Bioorganic Chemistry

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Hybrid nanophotonic–microfluidic sensor for highly sensitive liquid and gas analyses

Optics Letters (2022)

A. Yu. Kuzin Vasiliy S. Chernyshev Vadim Kovalyuk P An Alexander Golikov

Today, a lab-on-a-chip is one of the most promising ways to create sensor devices for gas and liquid analysis for environmental monitoring, early diagnosis, and treatment effectiveness assessment. On the one hand, this requires a large number of measurements and, on the other hand, involves minimum consumption of the test analytes. Combination of highly sensitive photonic integrated circuits (PICs) with microfluidic channels (MFCs) is necessary to solve this problem. In this work, PICs based on a silicon nitride platform integrated with MFCs for studying liquids and gases were developed. Different concentrations of isopropanol in de-ionized water were used as the analyte. Based on this, the sensitivity (S) and detection limit (DL) of the analyzed solution were evaluated. Entire system calibration was carried out to calculate S and DL, considering experimental and numerical simulation data. This development may be of interest as a promising platform for environmental monitoring and realization of point-of-care strategy for biomedical applications.

Lab-on-a-Chip

Realization (probability)

10.1364/ol.457309

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Citations (10)

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Journal of Visualized Experiments (2019)

Mikhail Skliar Vasiliy S. Chernyshev

Exosomes and other extracellular vesicles (EVs) are molecular complexes consisting of a lipid membrane vesicle, its surface decoration by membrane proteins and other molecules, and diverse luminal content inherited from a parent cell, which includes RNAs, proteins, and DNAs. The characterization of the hydrodynamic sizes of EVs, which depends on the size of the vesicle and its coronal layer formed by surface decorations, has become routine. For exosomes, the smallest of EVs, the relative difference between the hydrodynamic and vesicles sizes is significant. The characterization of vesicles sizes by the cryogenic transmission electron microscopy (cryo-TEM) imaging, a gold standard technique, remains a challenge due to the cost of the instrument, the expertise required to perform the sample preparation, imaging and data analysis, and a small number of particles often observed in images. A widely available and accessible alternative is the atomic force microscopy (AFM), which can produce versatile data on three-dimensional geometry, size, and other biophysical properties of extracellular vesicles. The developed protocol guides the users in utilizing this analytical tool and outlines the workflow for the analysis of EVs by the AFM, which includes the sample preparation for imaging EVs in hydrated or desiccated form, the electrostatic immobilization of vesicles on a substrate, data acquisition, its analysis, and interpretation. The representative results demonstrate that the fixation of EVs on the modified mica surface is predictable, customizable, and allows the user to obtain sizing results for a large number of vesicles. The vesicle sizing based on the AFM data was found to be consistent with the cryo-TEM imaging.

Nanoparticle tracking analysis

Characterization

Extracellular Vesicles

Extracellular vesicles

10.3791/59254

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Citations (45)

Surface tension of water in the presence of perfluorocarbon vapors

Soft Matter (2013)

Vasiliy S. Chernyshev Mikhail Skliar

Fluorocarbons are highly hydrophobic, biocompatible compounds with a variety of medical applications. Despite significant interest, the study of interfacial properties of fluorocarbons in aqueous systems has received limited attention. In this study, we investigate the influence of perfluoropentane and perfluorohexane vapors on the surface tension of water at room temperature. The results show a substantial decrease in the surface tension of water in the presence of perfluorocarbon vapors. In the investigated range of partial pressures up to the saturation value, a linear correlation between the surface tension and the partial pressure was found. This suggests that an adsorbed perfluorocarbon layer is formed on the surface of water. For comparison, the effect of the perfluorocarbon vapor on the surface tension of methanol was also investigated and a similar dependence was observed. Our results indicate that the stability and dynamic transitions of fluorocarbon colloids, which may be dispersed under physiological conditions as microdroplets, bubbles, or their combination, are likely affected by the composition of liquid and gas phases.

Fluorocarbon

Saturation (graph theory)

Atmospheric temperature range

10.1039/c3sm52289j

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Citations (20)

Gold nanoparticle-carbon nanotube multilayers on silica microspheres: Optoacoustic-Raman enhancement and potential biomedical applications

Materials Science and Engineering C (2020)

Daniil Nozdriukhin Nadezhda A. Besedina Vasiliy S. Chernyshev Olga Efimova Polina G. Rudakovskaya

Multiplex

Nanorod

10.1016/j.msec.2020.111736

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Citations (18)

Cyclic strain affects the orientation of endothelial tubulogenesis in a frequency‐dependent manner

The FASEB Journal (2006)

M. Iwamoto InSuk Joung J Tranum Jensen Vasiliy S. Chernyshev Yan-Ting E. Shiu

Angiogenesis occurs in a dynamic mechanical environment due to blood flow, but the role of hemodynamic forces in angiogenesis remains poorly understood. We recently developed a unique three-dimensional in vitro system for studying the tubulogenesis of vascular endothelial cells (EC) under well-defined cyclic strain (which mimics blood pressure-induced stretch). The objective of this study was to investigate how the frequency of cyclic strain affects EC tubulogenesis. Briefly, ECs were cultured to confluence on the top of a collagen gel. The EC monolayer was then stimulated for 1 day with basic fibroblast growth factor to induce tubulogenesis. Next, these cells were kept as controls or stretched at a 10% elongational strain for two days at two different frequencies: 1/12 or 1 Hz. Cord-like EC structures in the gel were monitored and analyzed with video microscopy and a commercial imaging software. We found that cyclic strain did not affect the number, depth, or length of the EC cords. However, cyclic strain induced the alignment of EC cords perpendicular to the principal axis of stretch; such an inductive effect was greater at a higher frequency. This in vitro system, along with the novel findings of strain-modulated endothelial tube morphology, enables the formation of an experimental basis for understanding the role of cyclic strain in the regulation of angiogenesis. (A part of this data has been accepted for publication in Microvascular Research. This project is supported by a research grant from the Whitaker Foundation to YTS.)

Strain (injury)

Mechanotransduction

10.1096/fasebj.20.4.a716-a

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Effect of Surface Modification of Multifunctional Nanocomposite Drug Delivery Carriers with DARPin on Their Biodistribution In Vitro and In Vivo

ACS Applied Bio Materials (2022)

Marina V. Novoselova Vasiliy S. Chernyshev Alexey Schulga Е. В. Коновалова Roman N. Chuprov‐Netochin

We present a targeted drug delivery system for therapy and diagnostics that is based on a combination of contrasting, cytotoxic, and cancer-cell-targeting properties of multifunctional carriers. The system uses multilayered polymer microcapsules loaded with magnetite and doxorubicin. Loading of magnetite nanoparticles into the polymer shell by freezing-induced loading (FIL) allowed the loading efficiency to be increased 5-fold, compared with the widely used layer-by-layer (LBL) assembly. FIL also improved the photoacoustic signal and particle mobility in a magnetic field gradient, a result unachievable by the LBL alone. For targeted delivery of the carriers to cancer cells, the carrier surface was modified with a designed ankyrin repeat protein (DARPin) directed toward the epithelial cell adhesion molecule (EpCAM). Flow cytometry measurements showed that the DARPin-coated capsules specifically interacted with the surface of EpCAM-overexpressing human cancer cells such as MCF7. In vivo and ex vivo biodistribution studies in FvB mice showed that the carrier surface modification with DARPin changed the biodistribution of the capsules toward epithelial cells. In particular, the capsules accumulated substantially in the lungs─a result that can be effectively used in targeted lung cancer therapy. The results of this work may aid in the further development of the "magic bullet" concept and may bring the quality of personalized medicine to another level.

Biodistribution

Surface Modification

Targeted drug delivery

10.1021/acsabm.2c00289

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Citations (6)

Identification and Analysis of Exosomes by Surface-Enhanced Raman Spectroscopy

Applied Sciences (2019)

Anastasiia Merdalimova Vasiliy S. Chernyshev Daniil Nozdriukhin Polina G. Rudakovskaya Dmitry A. Gorin

The concept of liquid biopsy has emerged as a novel approach for cancer screening, which is based on the analysis of circulating cancer biomarkers in body fluids. Among the various circulating cancer biomarkers, including Food and Drug Administration (FDA)-approved circulating tumor cells (CTC) and circulating tumor DNA (ctDNA), exosomes have attracted tremendous attention due to their ability to diagnose cancer in its early stages with high efficiency. Recently, surface-enhanced Raman spectroscopy (SERS) has been applied for the detection of cancer exosomes due to its high sensitivity, specificity, and multiplexing capability. In this article, we review recent progress in the development of SERS-based technologies for in vitro identification of circulating cancer exosomes. The accent is made on the detection strategies and interpretation of the SERS data. The problems of detecting cancer-derived exosomes from patient samples and future perspectives of SERS-based diagnostics are also discussed.

Liquid biopsy

Cancer Biomarkers

Surface-Enhanced Raman Spectroscopy

Cancer Detection

10.3390/app9061135

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Citations (36)

Engineered multicompartment vesicosomes for selective uptake by living cells

Colloids and Surfaces B Biointerfaces (2022)

Vasiliy S. Chernyshev Daniil Nozdriukhin Roman N. Chuprov‐Netochin Ekaterina Tsydenzhapova Marina V. Novoselova

Extracellular Vesicles

10.1016/j.colsurfb.2022.112953

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Citations (6)

Ultrasensitive Nanophotonic Random Spectrometer with Microfluidic Channels as a Sensor for Biological Applications

Nanomaterials (2022)

A. Yu. Kuzin Ilia M. Fradkin Vasiliy S. Chernyshev Vadim Kovalyuk P An

Spectrometers are widely used tools in chemical and biological sensing, material analysis, and light source characterization. However, an important characteristic of traditional spectrometers for biomedical applications is stable operation. It can be achieved due to high fabrication control during the development and stabilization of temperature and polarization of optical radiation during measurements. Temperature and polarization stabilization can be achieved through on-chip technology, and in turn robustness against fabrication imperfections through sensor design. Here, for the first time, we introduce a robust sensor based on a combination of nanophotonic random spectrometer and microfluidics (NRSM) for determining ultra-low concentrations of analyte in a solution. In order to study the sensor, we measure and analyze the spectra of different isopropanol solutions of known refractive indexes. Simple correlation analysis shows that the measured spectra shift with a tiny variation of the ambient liquid optical properties reaches a sensitivity of approximately 61.8 ± 2.3 nm/RIU. Robustness against fabrication imperfections leads to great scalability on a chip and the ability to operate in a huge spectral range from VIS to mid-IR. NRSM optical sensors are very promising for fast and efficient functionalization in the field of selective capture fluorescence-free oncological disease for liquid/gas biopsy in on-chip theranostics applications.

Nanophotonics

Lab-on-a-Chip

Robustness

10.3390/nano13010081

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Citations (2)

Membrane proteins significantly restrict exosome mobility

Biochemical and Biophysical Research Communications (2018)

Mikhail Skliar Vasiliy S. Chernyshev David M. Belnap German V. Sergey Samer M. Al-Hakami

Exosome

Cell membrane

10.1016/j.bbrc.2018.05.107

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Citations (68)