Wangzhang Li

Chinese Academy of Agricultural Sciences

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Xinyun Li

Huazhong Agricultural University

Yongsheng Zhang

Agricultural Genomics Institute at Shenzhen

Yilong Yao

Hunan University of Arts and Science

Yalan Yang

South China University of Technology

Fanqinyu Li

Agricultural Genomics Institute at Shenzhen

Wei Wang

China Medical University

Liu Yan-wen

University of Alabama at Birmingham

Zhonglin Tang

Chinese Academy of Agricultural Sciences

Mingsha Yang

Agricultural Genomics Institute at Shenzhen

Zishuai Wang

Chinese Academy of Agricultural Sciences

Cooperative Institutions

Agricultural Genomics Institute at Shenzhen

Chinese Academy of Agricultural Sciences

Huazhong Agricultural University

Ministry of Agriculture and Rural Affairs

China Medical University

Ministry of Agriculture Livestock and Fisheries

Ministry of Agriculture and Agro Based Industry

Shanxi Agricultural University

Wuhan University

Institute of Animal Sciences

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

MicroRNA-200c-5p Regulates Migration and Differentiation of Myoblasts via Targeting Adamts5 in Skeletal Muscle Regeneration and Myogenesis

International Journal of Molecular Sciences (2023)

Liu Yan-wen Yilong Yao Yongsheng Zhang Chao Yan Mingsha Yang

Skeletal muscle, as a regenerative organization, plays a vital role in physiological characteristics and homeostasis. However, the regulation mechanism of skeletal muscle regeneration is not entirely clear. miRNAs, as one of the regulatory factors, exert profound effects on regulating skeletal muscle regeneration and myogenesis. This study aimed to discover the regulatory function of important miRNA miR-200c-5p in skeletal muscle regeneration. In our study, miR-200c-5p increased at the early stage and peaked at first day during mouse skeletal muscle regeneration, which was also highly expressed in skeletal muscle of mouse tissue profile. Further, overexpression of miR-200c-5p promoted migration and inhibited differentiation of C2C12 myoblast, whereas inhibition of miR-200c-5p had the opposite effect. Bioinformatic analysis predicted that Adamts5 has potential binding sites for miR-200c-5p at 3’UTR region. Dual-luciferase and RIP assays further proved that Adamts5 is a target gene of miR-200c-5p. The expression patterns of miR-200c-5p and Adamts5 were opposite during the skeletal muscle regeneration. Moreover, miR-200c-5p can rescue the effects of Adamts5 in the C2C12 myoblast. In conclusion, miR-200c-5p might play a considerable function during skeletal muscle regeneration and myogenesis. These findings will provide a promising gene for promoting muscle health and candidate therapeutic target for skeletal muscle repair.

10.3390/ijms24054995

Cite

Citations (7)