Nada K. Alharbi

Jeddah University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Mayasar I. Al‐Zaban

Princess Nourah bint Abdulrahman University

Ahmed Ezzat Ahmed

King Khalid University

Ahlam H. AlRokban

Princess Nourah bint Abdulrahman University

Fawziah M. Albarakaty

Umm al-Qura University

Souheïla Naghmouchi

National Research Institute of Rural Engineering, Water and Forests

Maha A. Alharbi

Princess Nourah bint Abdulrahman University

Alyaa Abdelhameed

University of Diyala

Fatimah A. Al‐Saeed

King Khalid University

Gerry A. Quinn

University of Ulster

Ahmed M. Eid

Al-Azhar University

Cooperative Institutions

Zagazig University

Princess Nourah bint Abdulrahman University

Al-Azhar University

King Abdulaziz University

King Saud University

University of Faisalabad

University of Agriculture Faisalabad

King Khalid University

Mansoura University

Cairo University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Biological control of the root-knot nematode (Meloidogyne incognita) on eggplants with various chitinase-producing Streptomyces strains

European Journal of Plant Pathology (2023)

Amr M. Atif Fatma I. El-Zamik Gamal M. Mohamed Diana A. Al-Quwaie Mada F. Ashkan

Chitinase

Root-knot nematode

10.1007/s10658-023-02718-8

Cite

Citations (15)

Radiosynthesis, quality control, biodistribution, and infection-imaging study of a new 99mTc-labeled ertapenem radiopharmaceutical

Frontiers in Chemistry (2022)

Syed Ali Raza Naqvi Tania Jabbar Maha A. Alharbi Asma Noureen Nada K. Alharbi

Ertapenem is a member of carbapenem antibiotics used for the treatment of moderate-to-severe intra-abdominal, urinary tract, acute pelvic, and post-surgical gynecologic infections. The antibacterial activity of ertapenem is mediated through binding to penicillin-binding proteins which results in inhibiting the cross-linking of the peptidoglycan layer of the bacterial cell wall. Therefore, ertapenem can be labeled with technetium-99m ( 99m Tc), a gamma emitter radionuclide, for the diagnosis of deep-seated bacterial infections, such as urinary tract, intra-abdominal, osteomyelitis, and post-surgical gynecologic infections. The labeling procedure was carried out by varying the reaction conditions, such as the amount of the ligand and reducing agent, pH, reaction time and temperature, and radioactivity. At optimized reaction conditions more than 93% 99m Tc–ertapenem radioconjugate was obtained. 99m Tc–ertapenem was found 90% intact in saline medium up to 6 h, while 88% intact in human blood serum up to 3 h. Biodistribution study showed target-to-non-target ratios of 2.91 ± 0.19, 2.39 ± 0.31, and 1.23 ± 0.22 in S. aureus , E. coli , and turpentine oil-infected rat models, respectively. The SPECT scintigraphy showed high uptake of 99m Tc–ertapenem in bacterial-infected abscesses, and low counts were recorded in normal and turpentine oil-inflamed tissues. In conclusion, 99m Tc–ertapenem can be a potent infection-imaging agent, which can diagnosis deep-seated bacterial infections at early stage but need further pre-clinical evaluation in variety of infection models.

Ertapenem

Biodistribution

Carbapenem

10.3389/fchem.2022.1020387

Cite

Citations (4)

Proven anti-virulence therapies in combating methicillin- and vancomycin-resistant Staphylococcus aureus infections

Frontiers in Cellular and Infection Microbiology (2024)

Walid Bakeer Marwa Gaafar Ahmed O. El‐Gendy Mohamed. A. El Badry Mona G. Khalil

Introduction Despite years of efforts to develop new antibiotics for eradicating multidrug-resistant (MDR) and multi-virulent Methicillin-Resistant Staphylococcus aureus (MRSA) and Vancomycin-Resistant Staphylococcus aureus (VRSA) infections, treatment failures and poor prognoses in most cases have been common. Therefore, there is an urgent need for new therapeutic approaches targeting virulence arrays. Our aim is to discover new anti-virulence therapies targeting MRSA and VRSA virulence arrays. Methodology We employed phenotypic, molecular docking, and genetic studies to screen for anti-virulence activities among selected promising compounds: Coumarin, Simvastatin, and Ibuprofen. Results We found that nearly all detected MRSA and VRSA strains exhibited MDR and multi-virulent profiles. The molecular docking results aligned with the phenotypic and genetic assessments of virulence production. Biofilm and hemolysin productions were inhibited, and all virulence genes were downregulated upon treatment with sub-minimum inhibitory concentration (sub-MIC) of these promising compounds. Ibuprofen was the most active compound, exhibiting the highest inhibition and downregulation of virulence gene products. Moreover, in vivo and histopathological studies confirmed these results. Interestingly, we observed a significant decrease in wound area and improvements in re-epithelialization and tissue organization in the Ibuprofen and antimicrobial treated group compared with the group treated with antimicrobial alone. These findings support the idea that a combination of Ibuprofen and antimicrobial drugs may offer a promising new therapy for MRSA and VRSA infections. Conclusion We hope that our findings can be implemented in clinical practice to assist physicians in making the most suitable treatment decisions.

10.3389/fcimb.2024.1403219

Cite

Citations (0)

Kinetic, Isotherm and Thermodynamic Aspects of Zn2+ Biosorption by Spirulina platensis: Optimization of Process Variables by Response Surface Methodology

Life (2022)

Nada K. Alharbi Mayasar I. Al‐Zaban Fawziah M. Albarakaty Sayed F. Abdelwahab Sedky H.A. Hassan

The aim of this study was to assess the efficiency of Spirulina platensis for removing Zn2+ ions from the aqueous solutions. The optimized conditions of 4.48 g/L algal dose, pH of 6.62 and initial zinc concentration of 29.72 mg/L obtained by response surface methodology were employed for Zn2+ biosorption by S. platensis and up to 97.90% Zn2+ was removed, showing that there is a favorable harmony between the experimental data and model predictions. Different kinetic and equilibrium models were used to characterize the biosorption manner of Spirulina as a biosorbent. The kinetic manner of Zn2+ biosorption was well characterized by the pseudo-second-order, implying that the adsorption process is chemical in nature. The Langmuir and Dubinin-Radushkevich isotherm models were best fit to the equilibrium data. The maximum adsorption capacity of the Langmuir monolayer was 50.7 mg/g. Furthermore, the thermodynamic analysis revealed that Zn2+ biosorption was endothermic, spontaneous and feasible. As a result of biosorption process, FTIR, SEM, and EDX investigations indicated noticeable alterations in the algal biomass's properties. Therefore, the dried Spirulina biomass has been shown to be cost-effective and efficient for removing the heavy metals, particularly zinc ions from wastewater, and the method is practicable, and environmentally acceptable.

Biosorption

Endothermic process

Langmuir adsorption model

Spirulina (dietary supplement)

10.3390/life12040585

Cite

Citations (19)

Exploring phenotypic and genotypic diversity among methicillin-resistant, vancomycin-resistant, and sensitive Staphylococcus aureus

Medicine (2024)

Walid Bakeer Marwa Gaafar Ahmed O. El‐Gendy Mohamed. A. El Badry Noaf Abdullah N. Alblwi

Methicillin-Resistant Staphylococcus aureus (MRSA) is a global concern owing to the increasing prevalence of multidrug-resistant (MDR) strains. Vancomycin has been the primary treatment for MRSA; however, Vancomycin-resistant strains are being increasingly reported worldwide. Therefore, comparative studies are essential to support antimicrobial stewardship and improving clinical management. Ultimately, the findings from this study are expected to inform treatment strategies and guide public health interventions effectively. This study investigated the prevalence, antimicrobial resistance, and virulence characteristics of Vancomycin-sensitive S. aureus (VSSA) and Vancomycin-resistant S. aureus (VRSA) within MRSA strains. By employing a combination of phenotypic methods, such as antimicrobial susceptibility testing, and genotypic techniques, including molecular typing and identification of virulence genes, we obtained comprehensive insights into VRSA and VSSA profiles. Of 250 clinical samples, 62 (24.8%) were S. aureus and 27 (43.5%) were identified as MRSA. All MRSA isolates exhibited MDR patterns. Most MRSA strains were VSSA (20/27, 74.1%), while 7 (25.9%) were VRSA. The VRSA isolates showed more antimicrobial resistance than VSSA isolates; however, the VRSA isolates had less virulence than VSSA isolates. Linezolid was the most effective treatment, with a 3.7% resistance rate. A higher percentage of biofilm-producing MRSA (96.3%) was confirmed by both phenotypic and genotypic methods. All isolates, except one VRSA, showed multi-virulence patterns (harbored more than 3 virulence genes). High diversity and low clonality (D-value = 0.99) were found in both VSSA and VRSA. Based on our correlation findings, the emergence of vancomycin resistance could modify the association between antimicrobial resistance and virulence, potentially affecting the pathogenic profile of these strains. The study also revealed complex interactions among host factors (including age and gender), sample origin, antimicrobial resistance, biofilm production, and virulence genes. This study highlights the alarming spread of MRSA and VRSA, which show significant resistance and virulence.

10.1097/md.0000000000041051

Cite

Citations (0)

Prevalence, antibiotic profile, virulence determinants, ESBLs, and non-β-lactam encoding genes of MDR Proteus spp. isolated from infected dogs

Frontiers in Genetics (2022)

Reham M. El-Tarabili Elsayyad M. Ahmed Nada K. Alharbi Maha A. Alharbi Ahlam H. AlRokban

This study investigated the prevalence, antibiogram, virulence, extended-spectrum β-lactamases (ESBLs), and non-β-lactam encoding genes of Proteus species isolated from infected dogs in Ismailia province, Egypt. The study was conducted on 70 fecal swabs collected from dogs with diarrhea for bacteriological identification of Proteus spp. The positive isolates were evaluated for antibiotic susceptibility, molecular tests of virulence, ESBLs, and non-β-lactam encoding genes. Prevalence of Proteus spp. was 35.7% (25/70), including Proteus mirabilis (n = 23) and Proteus vulgaris (n = 2). The Proteus spp. prevalence revealed diversity, higher in males than females, in ages < 12 weeks. Investigation of antimicrobial resistance was found against penicillin and amoxicillin (100%), amoxicillin-clavulanic acid (32%), cephalosporins: cefotaxime and ceftazidime (36%), and monobactam: aztreonam (28%) as ESBLs, in addition to tetracycline (32%) and trimethoprim sulfamethoxazole (100%). The strains retrieved by PCR revealed ureC, zapA, and rsbA virulence genes with variant prevalence as 92%, 60%, and 52%, respectively. In addition, the recovered strains contained ESBL genes with a dramatic variable prevalence of 100%, 92%, 36%, and 32%, to blaTEM, blaSHV, blaCTX-M, and blaOXA-1, respectively, and non β-lactam encoding genes with a prevalence of 100%, 48%, 44%, 20%, and 12%, to sul1, tetA, intI1, qnrA, and aadA1. Moreover, 28% (7/25) of recovering strains were MDR (multidrug-resistant) up to four classes of antimicrobials, and 48% (12/25) of the examined strains were MDR up to three antimicrobial classes. In conclusion, to the best of our knowledge, our study could be the first report recording MDR Proteus spp. in dogs in Egypt.

Lactam

10.3389/fgene.2022.952689

Cite

Citations (2)

The Isolation of a Novel Streptomyces sp. CJ13 from a Traditional Irish Folk Medicine Alkaline Grassland Soil that Inhibits Multiresistant Pathogens and Yeasts

Applied Sciences (2020)

Gerry A. Quinn Alyaa Abdelhameed Nada K. Alharbi Diego Cobice Simms A. Adu

The World Health Organization recently stated that new sources of antibiotics are urgently required to stem the global spread of antibiotic resistance, especially in multiresistant Gram-negative bacteria. Although it was thought that many of the original sources of antibiotics were exhausted, innovative research has revealed promising new sources of antibiotic discovery in traditional medicine associated with Streptomyces. In this work we investigated the potential of a specific limestone grassland soil, associated with Irish folk medicine, as a new source of antimicrobial discovery. Using selective enrichment and isolation techniques on a limestone grassland soil sample obtained from Boho, West Fermanagh, we isolated Streptomyces sp. CJ13. This bacterium inhibited the growth of a broad range of pathogens in vitro including Gram positive Staphylococcus aureus (MRSA 43300) and Gram negative multiresistant Pseudomonas aeruginosa (PA01), as well as the anaerobic bacteria Propionibacterium acnes and the yeast Starmerella bombicola. Genome sequencing and phylogenetic analysis revealed Streptomyces sp. CJ13 to be closely related to an unclassified Streptomyces sp. MJM1172, Streptomyces sp. Mg1 and two species known as Streptomyces sp. ICC1 and ICC4 from a karst region in British Columbia. The closest type species to Streptomyces sp. CJ13 was Streptomyces lavendulae subspecies lavendulae. Analysis of Streptomyces sp. CJ13 whole genome sequence using the secondary metabolite prediction tool antiSMASH revealed similarities to several antibiotic gene synthesis clusters including salinichelin, mediomycin A, weishanmycin, combamide, heat stable antifungal factor and SAL-2242. These results demonstrate the potential of this alkaline grassland soil as a new resource for the discovery of a broad range of antimicrobial compounds including those effective against multiresistant Gram negative bacteria.

10.3390/app11010173

Cite

Citations (8)

Limoniastrum monopetalum–Mediated Nanoparticles and Biomedicines: In Silico Study and Molecular Prediction of Biomolecules

Molecules (2022)

Afrah E. Mohammed Sahar S. Alghamdi Nada K. Alharbi Fatma Alshehri Rasha Saad Suliman

An in silico approach applying computer-simulated models helps enhance biomedicines by sightseeing the pharmacology of potential therapeutics. Currently, an in silico study combined with in vitro assays investigated the antimicrobial ability of

Chlorogenic Acid

10.3390/molecules27228014

Cite

Citations (4)

Plant-Based Copper Oxide Nanoparticles; Biosynthesis, Characterization, Antibacterial Activity, Tanning Wastewater Treatment, and Heavy Metals Sorption

Catalysts (2023)

Ahmed M. Eid Amr Fouda Saad S. M. Hassan Mohammed F. Hamza Nada K. Alharbi

Herein, the aqueous extract of Portulaca oleracea has been used as a safe, cheap, eco-friendly, and applicable scale-up method to bio-fabricate copper oxide nanoparticles (CuO-NPs). The character of CuO-NPs were determined using UV-vis spectroscopy, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM), Energy dispersive X-ray(EDX), Dynamic light scattering (DLS), and zeta potential. Spherical and crystalline CuO-NPs with a size range of 5–30 nm at a maximum surface plasmon resonance of 275 nm were successfully fabricated. The main components of the green-synthesized particles were Cu and O with weight percentages of 49.92 and 28.45%, respectively. A Zeta-potential value of −24.6 mV was recorded for CuO-NPs, indicating their high stability. The plant-based CuO-NPs showed promising antimicrobial and catalytic activity in a dose-dependent manner. Results showed that the synthesized CuO-NPs had the efficacy to inhibit the growth of pathogens Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans with low MIC values in the ranges of 6.25–25 µg/mL. The highest decolorization percentages of tanning wastewater were attained under sunlight irradiation conditions at a concentration of 2.0 mg/mL after 200 min with percentages of 88.6 ± 1.5% compared to those which were recorded under dark conditions (70.3 ± 1.2%). The physicochemical parameters of tanning wastewater including total suspended solids (TSS), total dissolved solids (TDS), chemical oxygen demand (COD), biological oxygen demand (BOD), and conductivity under optimum conditions were significantly decreased with percentages of 95.2, 86.7, 91.4, 87.2, and 97.2%, respectively. Interestingly, the heavy metals including cobalt (Co), lead (Pb), nickel (Ni), cadmium (Cd), and chromium (Cr (VI)) decreased with percentages of 73.2, 80.8, 72.4, 64.4, and 91.4%, respectively, after treatment of tanning wastewater with CuO-NPs under optimum conditions. Overall, the plant-synthesized CuO-NPs that have antimicrobial and catalytic activities are considered a promising nano-catalyst and environmentally beneficial to wastewater treatment.

Zeta potential

Biochemical oxygen demand

10.3390/catal13020348

Cite

Citations (59)

Zinc oxide Nanoparticles, Biosynthesis, characterization and their potent photocatalytic degradation, and antioxidant activities

Journal of King Saud University - Science (2022)

Fawziah M. Albarakaty Mayasar I. Al‐Zaban Nada K. Alharbi Fatima S. Bagrwan Abeer R.M. Abd El-Aziz

This study aimed to biosynthesize zinc oxide nanoparticles (ZnO-NPs) using the seed extract of Moringa oleifera. The catalytic activity of the biosynthesized ZnO-NPs was examined as photocatalyst for the degradation methylene blue (MB) and their antioxidant activity by H2O2 assay were studied. The biosynthesized ZnO-NPs and their physicochemical properties investigated via UV–visible spectroscopy (UV–vis), Fourier transform infrared (FTIR) spectroscopy, Transmission electron microscopy (TEM), energy dispersive X-ray (EDX), X-ray diffraction analysis (XRD) analysis, and zeta potential was calculated using the Zetasizer nano. UV–Visible analysis of the biosynthesized nanoparticles revealed the characteristic a specific peak at 375 nm indicating the formation of ZnO-NPs. XRD study showed a distinctive diffraction peak indicating the formation of crystalline nanoparticles which matches to the spherical and hexagonal structure of ZnO-NPs. TEM results confirmed the formation of spherical and hexagonal ZnO-NPs and the size ranging between 25 and 30 nm. EDX analysis was used for the determination of elemental composition of biosynthesized ZnO-NPs which included zinc, oxygen and carbon. FTIR spectroscopy is useful to determine the available functional group from the phytochemical components implicated in the stabilization and reduction of ZnO-NPs. ZnO-NPs exhibited effective photocatalytic activity in degrading methylene blue (MB) and maximum photocatalytic activity (71 %) after 24 hrs. In addition, ZnO NPs exhibited high antioxidant activity against H2O2 free radicals scavenger. The biosynthesized ZnO-NPs have excellent MB dye degradation power and complete dye degradation was achieved within 24 hrs and synthesized ZnO-NPs showed improved antioxidant power. ZnO-NPs are good tools for industrial applications.

Zeta potential

Methylene blue

10.1016/j.jksus.2022.102434

Cite

Citations (27)