Jan Houška

Masaryk University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Z. Sova

University of Hradec Králové

K. Koudela

University Hospital Plzen

Josef Havel

Masaryk University

A Vrbenská

National Center for Tuberculosis and Lung Disease

D Trefný

Z. Němec

Czech University of Life Sciences Prague

Eladia María Peña‐Méndez

Universidad de La Laguna

MŻ

Marek Żyromski

Adam Mickiewicz University in Poznań

J Jícha

University of Hradec Králové

Josef Havel

Masaryk University

Cooperative Institutions

Masaryk University

Charles University

University of Pardubice

Brno University of Technology

Central European Institute of Technology

St. Anne's University Hospital Brno

Universidad de La Laguna

Czech Academy of Sciences

Jan Evangelista Purkyně University in Ústí nad Labem

Czech Technical University in Prague

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Effect of blood pre-served before drying with allylisothiocyanate on animals. 2. Trials with poultry.

D Trefný Z. Sova Jan Houška Jan Řehoř S. Petkov

Source

Cite

Citations (0)

The values of cholesterol, phospholipids and esterified fatty acids in WL chickens in the first month of life.

PubMed (1969)

Zd. Sova J Jícha K. Koudela Jan Houška A Vrbenská

Summary In 683 chickens divided into groups according to age, the values of cholesterol and phospholipids were determined in the first month of the postincubation ontogenesis, and in 519 chickens the values of the total esterified fatty acids were determined. Cholesterol was determined photometrically with ferric sulphate, phospholipids with ammonium molybdate and amino-aphto sulphonic acid, and total esterified fatty acids after conversion with hydroxylamine and hydroxamic acid. In the first 5 days after hatching higher values of cholesterol (500–670 mg.%), of esterified fatty acids (450–620 mg.%), and of phospholipids (350–377 mg.%) were found. These values did not decrease until the 7th day and became stabilized at 150–207 mg.% for cholesterol, 200–300 mg.% for total esterified fatty acids, and 145–225 mg.% for phospholipids. The rise in lipids in the first 5 days of life in the serum of chickens is caused by their transport from the yolk sac via the ductus vitellinus to the intestine, from where they are resorbed and cause the bright-yellow coloration of the liver of chickens at an age of from 5 to 7 days. Zusammenfassung Der Gehalt an Cholesterol, Phospholipiden und Fettsaureestern im Blut von WL-Kuken wahrend des ersten Lebensmonates 683 Kuken wurden nach dem Schlupf in 13 Altersgruppen (1–42 Tage) unterteilt. Bei den verschiedenen Gruppen wurden im Blutserum der Cholesterol-, Phospholipid- und Fettsaureestergehalt bestimmt. Der Cholesterolgehalt wurde photometrisch mit Eisen (III)-sulfat, der Gehalt an Phospholipiden mit Ammoniummolybdat und Aminoaphto-Sulfonsaure und derjenige der Fettsaureestern nach deren Konversion mit Hydroxylamin und Hydrox-aminsaure bestimmt. In den ersten 5 Tagen nach dem Schlupfen wurden hohere Werte an Cholesterol (500–670 mg%), Fettsaureestern (450–620 mg%) und Phospholipiden (350–577 mg%) gefunden. Bis zum 7. Tag verminderten sich diese Werte nicht, spater blieben die Gehalte bei folgenden Werten stabil: Cholesterol 150–207 mg%, Fettsaureester 200–300 mg%, Phospholipide 145 bis 225 mg%. Die erhohten Lipidwerte im Serum wahrend der ersten 5 Lebenstage sind auf den Transport der Lipide vom Dottersack durch den Ductus vitellinus (Ductus omphaloentericus) zum Darm zuruckzufuhren. Im Darm werden die Lipide resorbiert und verursachen die hellgelbe Farbe der Leber bei Kuken im Alter zwischen 5 und 7 Tagen. Resume La teneur en cholesterol, phospholipides et esters d'acides gras du sang de poussins WL pendant les premiers mois de la vie On repartit 683 poussins en 13 categories d'âge, de l'eclosion au 42eme jour. On determine dans ces categories la teneur en cholesterol, phospholipides et esters d'acides gras du serum sanguin. La determination du cholesterol se fait par photometrie a l'aide du sulfate de fer (III), celle des phospholipides a l'aide du molybdate d'ammonium et d'acide aminonaphtol-sulfonique et celle des esters d'acides gras, apres leur conversion, a l'aide d'hydroxylamine et d'acide hydroxamique. Les 5 premiers jours apres l'eclosion, on trouve des valeurs elevees en cholesterol (500–670 mg%), en esters d'acides gras (450–620 mg%) et en phospholipides (350–377 mg%). Ces valeurs demeurent inchangees jusqu'au 7eme jour; plus tard, elles se stabilisent a 150–207 mg% de cholesterol, 200–300 mg% d'esters d'acides gras et 145–225 mg% de phospholipides. Les valeurs elevees en lipides seriques pendant les 5 premiers jours de la vie peuvent etre attribuees au transport des lipides du sac vitellin jusqu'a l'intestin par le canal vitellin (Ductus omphaloentericus). Les lipides sont resorbes dans l'intestin et sont cause de la couleur jaune clair du foie des poussins âges de 5 a 7 jours. Resumen Los valores de colesterol, fosfolipidos y acidos grasos esterificados en la sangre de pollitos Leghorn blancos durante el primer mes de vida 683 pollitos se subdividieron tras la eclosion en 13 grupos de acuerdo con la edad (1–42 dias). En los distintos grupos se valoraron en el suero sanguineo los contenidos en colesterol, fosfolipidos y acidos grasos esterificados. El contenido en colesterol se valoro con la tecnica fotometrica a base de sulfato ferrico (III), el contenido en fosfolipidos con molibdato amonico y acido amino-afto-sulfonico, y el correspondiente a los acidos grasos esterificados tras su conversion con hidroxilamina y acido hidroxamico. En los 5 primeros dias tras la eclosion se hallaron valores superiores en colesterol (500–670 mg.%), acidos grasos esterificados (450–620 mg.%) y fosfolipidos (350–577 mg.%). Hasta el 7° dia no disminuian estos valores, permaneciendo los contenidos mas adelante estables con los valores siguientes: colesterol 150–207 mg.%, acidos grasos esterificados totales 200–300 mg.%, fosfolipidos 145–225 mg.%. Los valores aumentados de los lipidos en el suero sanguineo durante los 5 primeros dias de vida se atribuyen al transporte de los lipidos desde el saco vitelino a traves del conducto vitelino u onfaloenterico al intestino. En el intestino se resorben los lipidos y ocasionan la coloracion amarillo-clara del higado en los pollos que tienen una edad comprendida entre los 5 y 7 dias.

Yolk

Source

Cite

Citations (3)

Laser ablation of ternary As‐S‐Se glasses and time‐of‐flight mass spectrometric study

Rapid Communications in Mass Spectrometry (2009)

Sachinkumar Dagurao Pangavhane Jan Houška T. Wágner Martin Pavlišta Jan Janča

Abstract Ternary chalcogenide As‐S‐Se glasses, important for optics, computers, material science and technological applications, are often made by pulsed laser deposition (PLD) technology but the plasma composition formed during the process is mostly unknown. Therefore, the formation of clusters in a plasma plume from different glasses was followed by laser desorption ionization (LDI) or laser ablation (LA) time‐of‐flight mass spectrometry (TOF MS) in positive and negative ion modes. The LA of glasses of different composition leads to the formation of a number of binary As p S q , As p Se r and ternary As p S q Se r singly charged clusters. Series of clusters with the ratio As:chalcogen = 3:3 (As 3 S , As 3 S 2 Se + , As 3 SSe ), 3:4 (As 3 S , As 3 S 3 Se + , As 3 S 2 Se , As 3 SSe , As 3 Se ), 3:1 (As 3 S + , As 3 Se + ), and 3:2 (As 3 S , As 3 SSe + , As 3 Se ), formed from both bulk and PLD‐deposited nano‐layer glass, were detected. The stoichiometry of the As p S q Se r clusters was determined via isotopic envelope analysis and computer modeling. The structure of the clusters is discussed. Copyright © 2009 John Wiley & Sons, Ltd.

Stoichiometry

Chalcogen

Laser Ablation

Pulsed Laser Deposition

10.1002/rcm.4362

Cite

Citations (20)

CHEMISTRY OF GOLD CLUSTERS IN PLASMA GENERATED WITH MALDI, LASER DESORPTION IONISATION AND LASER ABLATION FROM VARIOUS PRECURSORS

Eladia María Peña‐Méndez J. R. Hernández-Fernaud NagenderReddy Panyala Jan Houška Josef Havel

Common MALDI TOF MS instrumentation can be used to analyze gold species ionized in plasma by laser from GNP synthesized by chemical procedures. Gold is forming in plasma positively and negatively single charged clusters Aum + or Aum - which are also reacting with the other ions and mixed AumNan, AuCa, AuK, etc. clusters were also observed. The formation of gold hydrides, such as AuH, Au3H, and hydroxide AuOH and peroxides Au- HOOH, were also observed here for the first time. When GNP were used as a matrix for ionization of nanodiamonds, carbon-gold mixed clusters which includes C18Au, CAu2Na and C2Au2Na, and others were detected.

Laser Ablation

Gold Compounds

Carbon fibers

Source

Cite

Citations (5)

[Uric acid in blood serum and liver of pheasants (Phasianus colchicus) up to the age of 1 year].

PubMed (1972)

Z. Sova K. Koudela D Trefný A Vrbenská Z. Němec

Phasianus

Blood serum

Source

Cite

Citations (0)

CCDC 734906: Experimental Crystal Structure Determination

The Cambridge Structural Database (2011)

Marie Sotolářová Jan Houška Marek Nečas Josef Havel Jiřı́ Přı́hoda

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

10.5517/ccsnqns

Cite

Citations (0)

Laser desorption ionization of red phosphorus clusters andtheir use for mass calibration in time-of-flight mass

Rapid Communications in Mass Spectrometry (2009)

Kateřina Sládková Jan Houška Josef Havel

Phosphorus clusters P-n (n = 1-89) are easily formed from red phosphorus by laser desorption ionization (LDI) and they cover a range of up to approx. m/z 3000 in both positive and negative ion mode. The clusters are singly charged and the spectra are simple because phosphorus is monoisotopic. The mass spectra can be measured with an acceptable resolution and intensity. The use of positively charged P-n clusters for calibration in mass spectrometry was examined and it was demonstrated that in external calibration a standard deviation of +/-0.04 m/z units can be achieved even when using a common commercial matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instrument. When used as internal standards the P-n clusters react with some analytes - C-60 and C-70 fullerenes and cucurbituril[8], for example. It was also found that red phosphorus is a suitable MALDI matrix for peptides and proteins, illustrated by the examples of a Calmix mixture of bradykinin, angiotensin, renin, adrenocorticotropic hormone ACTH fragment 18-359 and insulin, and of insulin alone.

Monoisotopic mass

Matrix (chemical analysis)

Source

Cite

Citations (0)

REAL TIME TOOLBOX FOR PC-MATLAB

Elsevier eBooks (1992)

Jan Houška Bohumír Sládek

Toolbox

10.1016/b978-0-08-041894-0.50038-1

Cite

Citations (0)

[Mineral substances (Na, K, Cl, Ca, P) in blood serum of 4 breeds of geese kept in Czechoslovakia].

PubMed (1972)

Z. Sova J Jícha D Trefný Jan Houška K. Koudela

Blood serum

Source

Cite

Citations (0)

Laser ablation time-of-flight mass spectrometry (LA-TOF-MS) ofsilicon doped diamond-like carbon (DLC-Si)

Jan Houška Vilma Buršı́ková Josef Havel

Silicon-doped diamond-like carbon (DLC-Si) was prepared by plasma-enhanced chemical vapor deposition (PECVD) technique from a mixture of hexamethyldisiloxane (HMDSO) and oxygen using dual frequenci capacitive discharge. Time of flight mass spectrometer equipped with nitrogen laser (337 nm) was used to charakterize its structure via laser ablation. Stoichometry of positively or negatively charged species was confirmed via isotopic pattern simulation. DLC-Si material shows typical spectral patterns similar to those diamond-like carbon. In addition, chemical bound of Si-C in this material was proved and many positively or negatively charged O(m)Si(n)C(o)H(p) species were identified in plasma plume.

Hexamethyldisiloxane

Diamond-like carbon

Laser Ablation

Carbon fibers

Source

Cite

Citations (0)