Margherita Profiti

University of Turin

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Co-Authors

Sergio Rosati

University of Turin

Luigi Bertolotti

University of Turin

Chiara Nogarol

Bitron (Italy)

Ramsés Reina

Agrobiotechnology Institute

Elena Grego

University of Turin

Elvira Muratore

Cairo University

Loretta Masoero

Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta

Barbara Colitti

University of Turin

M. Juganaru

Camera de Comerț Industrie și Agricultură Timișoara

Daniela Lacerenza

University of Turin

Cooperative Institutions

University of Turin

Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta

University of Pisa

Istituto Zooprofilattico Sperimentale delle Venezie

University of Milan

Istituto Superiore di Sanità

University of Padua

University of Bari Aldo Moro

Torino e-district

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"

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Field application of an indirect gE ELISA on pooled milk samples for the control of IBR in free and marker vaccinated dairy herds

BMC Veterinary Research (2018)

Barbara Colitti Elvira Muratore Maria Elena Careddu Luigi Bertolotti Bryan Iotti

The aim of the present study was to assess the reliability of a new strategy for monitoring the serological response against Bovine Herpesvirus 1 (BoHV1), the causative agent of infectious bovine rhinotracheitis (IBR). Bulk milk samples have already been identified as cost effective diagnostic matrices for monitoring purposes. Nevertheless, most eradication programs are still based on individual standard assays. In a region of northwestern Italy (Piedmont), the voluntary eradication program for IBR has become economically unsustainable. Being the prevalence of infection still high, glycoprotein E-deleted marker vaccines are commonly used but gE blocking ELISAs are less sensitive on bulk milk samples compared to blood serum. A recently developed indirect gE ELISA showed high versatility when applied to a wide range of matrices. In this study, we applied a faster, cost effective system for the concentration of IgG from pooled milk samples. The IgG enriched fractions were tested using a gE indirect ELISA for monitoring purposes in IBR-positive and IBR-marker-vaccinated herds. Official diagnostic tests were used as gold standard. During a 3 years study, a total 250 herds were involved, including more than 34,500 lactating cows. The proposed method showed a very good agreement with official diagnostic protocols and very good diagnostic performances: only 37 positive animals were not detected across the entire study. The results highlighted the ability of the proposed method to support the surveillance of IBR in the Piedmont region, reducing the costs without affecting the diagnostic performances.

Bovine herpesvirus 1

Gold standard (test)

Infectious bovine rhinotracheitis

Bulk tank

10.1186/s12917-018-1716-5

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Citations (11)

Development of recombinant capsid antigen/transmembrane epitope fusion proteins for serological diagnosis of animal lentivirus infections

Journal of Virological Methods (2004)

Sergio Rosati Margherita Profiti R. Lorenzetti Patrizia Bandecchi Alessandro Mannelli

Feline immunodeficiency virus

Equine infectious anemia

Epitope mapping

10.1016/j.jviromet.2004.06.001

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Citations (42)

Molecular and Antigenic Characterization of aMycoplasma BovisStrain Causing an Outbreak of Infectious Keratoconjunctivitis

Journal of Veterinary Diagnostic Investigation (2006)

Alberto Alberti Maria Filippa Addis Bernardo Chessa Tiziana Cubeddu Margherita Profiti

An unusually high incidence of infectious keratoconjunctivitis followed by pneumonia and arthritis was observed in beef calves of a managed herd. No Moraxella spp. or bacteria other than Mycoplasma spp. were obtained from conjunctival and nasal swabs. A strategy was designed for characterization of bovine mycoplasmas at species and strain level on the basis of a combination of molecular tools and the immunoblotting method. The strategy made it possible to rapidly assign the bacterium responsible for this outbreak to one of the phylogenetic clusters of bovine mycoplasmas delineated in this study and then to identify it as Mycoplasma bovis. The strain, designated Sar 1, showed a 100% 16S rDNA sequence identity with two European strains (120/81 and MC3386) isolated in Germany and Ireland, respectively, and hosts a vsp gene analog to the vspA, vsp422-4, and vsp422-8 genes of the M. bovis reference strain PG45T and of the field strain 422. The use of a cross-reactive rabbit serum developed against the Mycoplasma agalactiae immunodominant antigen P48 confirmed the molecular findings. The immunological response of calves against M. bovis was also investigated. This is the first report on the occurrence of M. bovis on the Island of Sardinia (Italy).

Keratoconjunctivitis

Strain (injury)

10.1177/104063870601800106

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Genetic characterization of small ruminant lentivirus genotype E.

Ramsés Reina Elena Grego Daniela Lacerenza Margherita Profiti Sergio Rosati

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A versatile and sensitive lateral flow immunoassay for the rapid diagnosis of visceral leishmaniasis

Analytical and Bioanalytical Chemistry (2018)