Y. C. Zee

University of California, San Diego

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John W. Osebold

University of California, Davis

R. F. Pritchett

University of California, Davis

L. T. Talens

Washington State University

A. J. Hackett

Peralta Community College District

David S. Hsu

Duke Medical Center

Laurel J. Gershwin

University of California, Davis

David C. Bolton

New York State Office for People With Developmental Disabilities

Ling‐Na Shih

Ministry of Health and Welfare

Alex Ardans

University of California, Davis

Anthony E. Castro

California State University, San Bernardino

Cooperative Institutions

University of California, Davis

University of California, Berkeley

Saint Louis University

Stanford University

University of California, San Francisco

University Medical Center

Veterinary Medical Teaching Hospital

University at Buffalo, State University of New York

National Chung Hsing University

National Institutes of Health

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Localization of a small ribonucleic acid virus within cytoplasmic cisternae

Journal of Virology (1967)

Y. C. Zee L. T. Talens A. J. Hackett

10.1128/jvi.1.6.1271-1273.1967

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Citations (6)

Dynamics of B-lymphocytes in the lungs of mice exposed to aerosolized influenza virus

Infection and Immunity (1981)

Sally Owens John W. Osebold Y. C. Zee

Immunoglobulin-containing cells were revealed by immunofluorescence in lung sections from mice infected with influenza virus by the aerosol route. The numbers of immunoglobulin A (IgA)- and IgM-containing cells were increasing by day 3 of the infection, whereas IgG-containing cells appeared a few days later. The responding B-cell populations appeared in two principal locations: along major airways and in consolidated lesions within lung parenchyma. IgA-containing cells were the most numerous isotype, occurring predominantly in the lamina propria of the airways. IgG-containing cells were the least frequently encountered class along airways and appeared most often within consolidated lung lesions in clustered groupings. Cells staining for mu chain appeared along the airways and in lung lesions. The population of IgM-containing cells declined approximately 30 days after infection. Cells producing alpha and gamma chains were still numerous on day 46. Assays for virus-reactive antibodies in lung secretions were positive on day 8 of the infection. The IgM titers were the first to decline, but virus-binding antibodies for all classes were still present on day 33. The implications of immune responses in viral pneumonitis were considered.

Isotype

Parenchyma

Immunofluorescence

Immunoglobulin M

Hypersensitivity pneumonitis

10.1128/iai.33.1.231-238.1981

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Citations (24)

Immunological Alterations in the Lungs of Mice following Ozone Exposure: Changes in Immunoglobulin Levels and Antibody-Containing Cells

Archives of Environmental Health An International Journal (1979)

John W. Osebold Sally Owens Y. C. Zee William M. Dotson David D. LaBarre

Abstract Ozone was added to the air of environmental chambers containing specific pathogen-free mice. At levels of 0.5 and 0.8 ppm the oxidant was seen to have inflammatory effects, as shown by rising serum albumin levels in lung lavage fluid. Fluorescein conjugated anti-heavy chain sera were used to detect cells containing IgM, IgG, and IgA in measured lung areas termed Pulmonary Units. Antigenic stimuli occurred along the airways, with significant increases of IgA-containing cells in the bronchus-associated lymphoid tissue. The numbers of IgM- and IgG-containing cells did not increase. Immunodiffusion analyses for immunoglobulins in lung lavage fluid indicated increases of IgG1 IgG2, and IgA in lung secretions. The calculation of changing Ig/Alb ratios suggested that the IgA present was largely the result of local synthesis, while IgG molecules were mainly of serum origin. Possible sources of the antigenic stimuli to ozone-exposed lungs are discussed.

Immunodiffusion

Immunoglobulin A

Immunoglobulin M

Radial immunodiffusion

10.1080/00039896.1979.10667411

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Citations (35)

Complete nucleotide and deduced amino acid sequence of genome segment 5 encoding the outer capsid protein, VP5, of a U.S. isolate of bluetongue virus serotype 11

Virus Research (1991)

Stephen J. Dunn David S. Hsu Y. C. Zee Jeffrey L. Stott

Orbivirus

10.1016/0168-1702(91)90005-g

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Citations (4)

Enzyme-linked immunosorbent assay for the detection of antibodies to bovine viral diarrhea virus in bovine sera

Veterinary Microbiology (1985)

H. P. Chu Y. C. Zee Alex Ardans Kang Dai

Pestivirus

10.1016/0378-1135(85)90003-3

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Citations (46)

Neutralizing monoclonal antibodies directed to infectious bovine rhinotracheitis virus

Archives of Virology (1986)

Li‐Wen Chang Y. C. Zee R. F. Pritchett Alex Ardans

Immunoprecipitation

Infectivity

10.1007/bf01310875

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Citations (15)

Immunofluorescence Determination of the Pathogenesis of Infection with Influenza Virus in Mice Following Exposure to Aerosolized Virus

The Journal of Infectious Diseases (1979)

Tilahun Yilma Y. C. Zee John W. Osebold

The pathogenesis of infection with influenza A virus in mice was studied by exposure of specific pathogen-free mice to aerosols of influenza virus and by monitoring of mortality, viral titers in lung homogenates, and presence of viral antigens in respiratory cells as determined by immunofluorescence. In two experiments with different death rates (100% and 43%), viral antigen accumulated in the epithelial cells lining the airways, in alveolar macrophages, in alveolar cells, and in visceral pleura. By enumeration of the number of airways, alveolar macrophages, and alveolar cells containing influenza viral antigens at different intervals after exposure to the viral aerosol, it was determined that viral replication occurred initially in the epithelial cells lining the airways and later extended to the alveolar macrophages and alveolar cells. This semiquantitative survey of the dynamics of influenza viral infection by aerosol indicated that the viral infection in mice was a descending process.

Immunofluorescence

Pathogenesis

Viral Pathogenesis

10.1093/infdis/139.4.458

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Citations (25)

Nucleotide sequence of a 3.5 kilobase fragment of malignant catarrhal fever virus strain WC11

Archives of Virology (1990)

David S. Hsu Ling‐Na Shih Y. C. Zee

pUC19

10.1007/bf01318352

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Citations (6)

Immunoglobulin E-Containing Cells in Mouse Lung Following Allergen Inhalation and Ozone Exposure

International Archives of Allergy and Immunology (1981)

Laurel J. Gershwin John W. Osebold Y. C. Zee

Cells containing immunoglobulin E (IgE) were enumerated and their location in mouse lungs was determined by direct immunofluorescence. Lungs were studied from mice that had been immunized with aerosolized ovalbumin as well as from normal mice and from mice that were exposed to ozone (0.5 or 0.8 ppm) prior to receiving aerosolized antigen. In addition, some mice were immunized intraperitoneally with ovalbumin precipitated in alum. IgE-containing cells were primarily airway-related in normal mice and in mice immunized by the intraperitoneal route. Lungs from aerosol-immunized, and aerosol-immunized and ozone-exposed mice showed a more disseminated distribution of IgE-containing cells. Fluorescent cells were counted and numbers were expressed as total cells per square millimeter of lung tissue and as airway-associated cells per millimeter of airway. Total IgE cells increased 9.4-fold in mice that received aerosolized ovalbumin as compared to normal mice. When ozone exposure was added to the effects from aerosolized ovalbumin, the increase of IgE cells over normal was 34.2-fold. IgE cell counts correlated well with anaphylactic sensitivity to intravenous challenge with ovalbumin. The observed enhancement of allergic sensitization by ozone exposure has important implications for human health.

Inhalation exposure

10.1159/000232766

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Citations (57)

Vesicular Stomatitis Virus Maturation Sites in Six Different Host Cells

Journal of General Virology (1970)

Y. C. Zee A. J. Hackett L. T. Talens

SUMMARY Six different cell types, L, Vero, HeLa, BHK21, PK(H13) and CF, were infected with vesicular stomatitis virus. A minimum of 100 individual virus-containing cells of each type was scored for the sites of viral maturation as observed by electron microscopy of thin sections. The principal site of viral maturation was the intracytoplasmic vacuolar membranes for PK(H13) and the plasma membrane for L and Vero cells. Both types of membranes served as sites for HeLa and BHK21 cells.It is concluded that the site of maturation of vesicular stomatitis virus is a host-dependent phenomenon.

Vero cell

Vesicular Stomatitis

HeLa

10.1099/0022-1317-7-2-95

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Citations (49)