Peilei Cheng

Suzhou Polytechnic Institute of Agriculture

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Sumei Chen

Ministry of Agriculture and Rural Affairs

Jiafu Jiang

Nanjing Agricultural University

Fadi Chen

Ministry of Agriculture and Rural Affairs

Weimin Fang

Nanjing Agricultural University

Zixin Zhang

Johns Hopkins Medicine

Yanan Liu

Fujian Agriculture and Forestry University

Changbing Huang

Suzhou Polytechnic Institute of Agriculture

Hua Cheng

Ministry of Agriculture and Rural Affairs

Jiaojiao Gao

Tongling University

Zhiyong Guan

Nanjing Agricultural University

Cooperative Institutions

Nanjing Agricultural University

Ministry of Agriculture and Rural Affairs

Chinese Academy of Sciences

State Forestry and Grassland Administration

Suzhou Polytechnic Institute of Agriculture

University of Chinese Academy of Sciences

Zhongshan Hospital

China Agricultural University

Peking University

Institute of Genetics and Developmental Biology

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat

Molecular Plant (2021)

Shujuan Xu Qi Dong Min Deng Dexing Lin Jun Xiao

Vernalization

Transcription

10.1016/j.molp.2021.05.026

Cite

Citations (59)

Whole genome duplication enhances the photosynthetic capacity of Chrysanthemum nankingense

Molecular Genetics and Genomics (2017)

Bin Dong Haibin Wang Tao Liu Peilei Cheng Yun Chen

Photosynthetic efficiency

Photosynthetic capacity

10.1007/s00438-017-1344-y

Cite

Citations (17)

Identification and differential expression of cold-stress-responsive microRNAs in cold-tolerant and -susceptible Hemerocallis fulva varieties

New Zealand Journal of Crop and Horticultural Science (2022)

Changbing Huang Peilei Cheng Huanchao Zhang Chun Jiang Limin Jin

Exploring microRNA (miRNA) expression patterns in Hemerocallis under low-temperature stress helps understanding the mechanisms of miRNA-mediated stress responses. Here, eight separate libraries were generated from the leaves and roots of two different varieties, cold-tolerant 'Jinyan 7' and cold-sensitive 'Lucretius 4', which were exposed to 20°C and −4°C for 8 h. In total, 333 cold-responsive miRNAs, including 85 putative novel and 248 conserved miRNAs, were identified during different temperature treatments. Under cold-stress conditions, two miRNAs were down-regulated and one was up-regulated in the roots, and no differentially expressed miRNAs were detected in the leaves in 'Lucretius 4'. In 'Jinyan 7', there were two novel and one conserved differentially expressed miRNAs in leaves, whereas three conserved and two novel miRNAs were differentially expressed in roots. The target genes were mainly involved in metabolic processes and cellular components. The differential expression levels of 10 miRNAs were validated using quantitative RT–PCR. Some candidate miRNAs (e.g. ath-miR165a, ath-miR166a, ath-miR395a and novel-miR67) may be involved in plant response-related processes to low-temperature.

Cold stress

10.1080/01140671.2021.2013260

Cite

Citations (3)

A Transcriptomic Analysis Targeting Genes Involved in the Floral Transition of Winter-Flowering Chrysanthemum

Journal of Plant Growth Regulation (2017)

Peilei Cheng Bin Dong Heng Wang Peipei Cao Tao Liu

Flowering Locus C

RNA-Seq

MADS-box

10.1007/s00344-017-9720-8

Cite

Citations (9)

Strigolactone represses the synthesis of melatonin, thereby inducing floral transition in Arabidopsis thaliana in an FLC‐dependent manner

Journal of Pineal Research (2019)

Zixin Zhang Qian Hu Yanan Liu Peilei Cheng Hua Cheng

The transition from vegetative to reproductive growth is a key developmental event in a plant's life cycle. The process is mediated by a combination of phytohormones, including melatonin (MT) and strigolactone (SL). Here, the Arabidopsis mutants, d14-1 and max4-1, which are compromised with respect to either SL synthesis or signaling, were shown to flower earlier than wild types. The tissue MT content in both mutants was higher than in wild types, as a result of the up-regulation of various genes encoding enzymes involved in MT synthesis. The abundance in the mutants of transcripts derived from each of the genes SPLs, AP1, and SOC1 was reduced with exogenously supplied MT, while FLC was induced. Plants exposed to a high concentration of MT did not flower earlier than wild types. The tissue MT content of a mutant unable to synthesize caffeic acid O-methyltransferase was less than that of wild type and flowered earlier than did wild types. The suggestion is that the flowering time of Arabidopsis is altered if the tissue content of MT is either higher than ~ 8 ng/g F.W, or lower than ~ 0.9 ng/g. Within this range, SL acts to determine flowering time by its regulation of SPL genes. The application of exogenous SL reduces tissue MT content. The flowering time of the flc-3 mutant was unaffected by exogenously supplying either MT or/and SL. It is proposed that MT acts downstream of SL to activate FLC, inducing a delay to flowering if its concentration lies outside a certain range.

Strigolactone

Wild type

10.1111/jpi.12582

Cite

Citations (58)

CmBES1 is a regulator of boundary formation in chrysanthemum ray florets

Horticulture Research (2020)

Peilei Cheng Yanan Liu Yiman Yang Hong Chen Hua Cheng

Chrysanthemum (Chrysanthemum morifolium) is an ideal model species for studying petal morphogenesis because of the diversity in the flower form across varieties; however, the molecular mechanisms underlying petal development are poorly understood. Here, we show that the brassinosteroid transcription factor BRI1-EMS-SUPPRESSOR 1 (CmBES1) in chrysanthemum (C. morifolium cv. Jinba) is important for organ boundary formation because it represses organ boundary identity genes. Chrysanthemum plants overexpressing CmBES1 displayed increased fusion of the outermost ray florets due to the loss of differentiation of the two dorsal petals, which developed simultaneously with the ventral petals. RNA-seq analysis of the overexpression lines revealed potential genes and pathways involved in petal development, such as CUP-SHAPED COTYLEDON (CUC2), CYCLOIDEA 4 (CYC4), genes encoding MADS-box transcription factors and homeodomain-leucine zippers (HD-Zips) and auxin pathway-related genes. This study characterizes the role of CmBES1 in ray floret development by its modulation of flower development and boundary identity genes in chrysanthemum.

Petal

MADS-box

Sepal

Chrysanthemum morifolium

Phyllotaxis

10.1038/s41438-020-00351-8

Cite

Citations (18)

The Cm14-3-3μ protein and CCT transcription factor CmNRRa delay flowering in chrysanthemum

Journal of Experimental Botany (2023)

Hua Cheng Jiaxin Zhang Yu Zhang Chaona Si Juanjuan Wang

Abstract The floral transition from vegetative to reproductive growth is pivotal in the plant life cycle. NUTRITION RESPONSE AND ROOT GROWTH (OsNRRa), as a CONSTANS, CONSTANS-LIKE, TOC1 (CCT) domain protein, delays flowering in rice, and an orthologous protein, CmNRRa, inhibits flowering in chrysanthemum; however, the underlying mechanism remains unknown. In this study, using yeast two-hybrid screening, we identified the 14-3-3 protein family member Cm14-3-3µ as a CmNRRa-interacting protein. A combination of bimolecular fluorescence complementation, pull-down, and co-immunoprecipitation assays was performed to confirm the physical interaction between CmNRRa and Cm14-3-3µ. In addition, expression analysis showed that CmNRRa but not Cm14-3-3µ responded to the diurnal rhythm, whereas both genes were highly expressed in leaves. Moreover, the function of Cm14-3-3µ in flowering time regulation was similar to that of CmNRRa. Furthermore, CmNRRa repressed chrysanthemum FLOWERING LOCUS T-like 3 (CmFTL3) and an APETALA 1 (AP1)/FRUITFULL (FUL)-like gene (CmAFL1) but induced TERMINAL FLOWER1 (CmTFL1) directly by binding to their promoters. Cm14-3-3µ enhanced the ability of CmNRRa to regulate the expression of these genes. These findings suggest that there is a synergistic relationship between CmNRRa and Cm14-3-3µ in flowering repression in chrysanthemum.

Bimolecular fluorescence complementation

Two-hybrid screening

Protein-fragment complementation assay

Chrysanthemum morifolium

10.1093/jxb/erad130

Cite

Citations (4)

Identification and differential expression of cold-stress-responsive microRNAs in cold-tolerant and -susceptible Hemerocallis fulva varieties

Figshare (2022)

Changbing Huang Peilei Cheng Huanchao Zhang Chun Jiang Limin Jin

Exploring microRNA (miRNA) expression patterns in Hemerocallis under low-temperature stress helps understanding the mechanisms of miRNA-mediated stress responses. Here, eight separate libraries were generated from the leaves and roots of two different varieties, cold-tolerant ‘Jinyan 7’ and cold-sensitive ‘Lucretius 4’, which were exposed to 20°C and −4°C for 8 h. In total, 333 cold-responsive miRNAs, including 85 putative novel and 248 conserved miRNAs, were identified during different temperature treatments. Under cold-stress conditions, two miRNAs were down-regulated and one was up-regulated in the roots, and no differentially expressed miRNAs were detected in the leaves in ‘Lucretius 4’. In ‘Jinyan 7’, there were two novel and one conserved differentially expressed miRNAs in leaves, whereas three conserved and two novel miRNAs were differentially expressed in roots. The target genes were mainly involved in metabolic processes and cellular components. The differential expression levels of 10 miRNAs were validated using quantitative RT–PCR. Some candidate miRNAs (e.g. ath-miR165a, ath-miR166a, ath-miR395a and novel-miR67) may be involved in plant response-related processes to low-temperature.

Identification

Cold stress

10.6084/m9.figshare.19128099

Cite

Citations (0)

Identification of WRKY transcription factors in Rosa chinensis and analysis of their expression response to alkali stress response

Functional Plant Biology (2024)

Changbing Huang Wenhui Cheng Feng Yu Tongyu Zhang Taotao Yan

Breeding abiotic stress-tolerant varieties of Rosa chinensis is a paramount goal in horticulture. WRKY transcription factors, pivotal in plant responses to diverse stressors, offer potential targets for enhancing stress resilience in R. chinensis. Using bioinformatics and genomic data, we identified RcWRKY transcription factor genes, characterised their chromosomal distribution, phylogenetic relationships, structural attributes, collinearity, and expression patterns in response to saline stress. Leveraging bidirectional database searches, we pinpointed 66 RcWRKY genes, categorised into three groups. All except RcWRKY60 encoded DNA Binding Domain and Zinc Finger Motif regions of the WRKY domain. Expansion of the RcWRKY gene family was propelled by 19 segmental, and 2 tandem, duplications. We unveiled 41 and 15 RcWRKY genes corresponding to 50 AtWRKY and 17 OsWRKY orthologs respectively, indicating postdivergence expansion. Expression analyses under alkaline stress pinpointed significant alterations in 54 RcWRKY genes. Integration of functional roles from their Arabidopsis orthologs and cis-acting elements within their promoters, along with quantitative reverse transcription PCR validation, underscored the importance of RcWRKY27 and 29 in R. chinensis’ alkaline stress response. These findings offer insights into the biological roles of RcWRKY transcription factors, as well as the regulatory dynamics governing R. chinensis’ growth, development, and stress resilience.

WRKY protein domain

Transcription

10.1071/fp23077

Cite

Citations (0)

Overexpression ofCmSOS1confers waterlogging tolerance inChrysanthemum

Journal of Integrative Plant Biology (2019)

Lijun Wang Jiaojiao Gao Zixin Zhang Weimiao Liu Peilei Cheng

The Na+/H+ antiporter SOS1 enhances the salinity tolerance of a number of plant species, but its involvement in the response to hypoxia is less well known. We presented chrysanthemum homologs CmSOS1 and CmRCD1 coordinately mediate waterlogging tolerance by maintaining membrane integrity and minimizing the level of reactive oxygen species. Additional Supporting Information may be found online in the supporting information tab for this article: http://onlinelibrary.wiley.com/doi/10.1111/jipb.12889/suppinfo Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.

Waterlogging (archaeology)

10.1111/jipb.12889

Cite

Citations (21)