Masaharu Iha

Okinawa Red Cross Hospital

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Research Field

Orosomucoid (ORM) Typing by Isoelectric Focusing: An Analysis of ORM Haplotypes

Human Heredity (1990)

Isao Yuasa Kazuo Umetsu Kazuyuki Suenaga Masaharu Iha Hisao Hirata

A new separator isoelectric focusing method for typing of orosomucoid (ORM) was developed. This method provided a superior resolution of ORM patterns: two close bands of ORM1*5.2 products were clearly separated. A total of 364 subjects from Okinawa (Japan) were classified into 21 ORM phenotypes determined by 6 ORM1 and 7 ORM2 alleles including a polymorphic silent allele, ORM2*QO, and 2 new rare variants, ORM2*18 and ORM2*19. These phenotypes were also explained by 12 ORM haplotypes, half of which were polymorphic.

Orosomucoid

10.1159/000153943

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Citations (9)

Factor I (C3b Inactivator) Polymorphism among Five Populations in Eurasia

Human Heredity (1988)

Isao Yuasa K. Umetsu Kazuyuki Suenaga Kikukatsu Ito Masaharu Iha

Factor I (C3b inactivator) polymorphism in the Japanese (in Western and Southern Japan), Taiwanese, Nepalese and French was studied using isoelectric focusing on polyacrylamide gels. The exposure of passively blotted nitrocellulose membranes to glutaraldehyde vapor facilitated the subsequent immunodetection of a low concentration of factor I and permitted the reliable identification of the three phenotypes determined by two codominant alleles F1*A and FI*B. The data indicated a west-to-east genocline, ranging from France to Western Japan, in which FI*A changed from 0.006 to 0.120.

Glutaraldehyde

10.1159/000153764

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Citations (6)

HLA genes and haplotypes in Ryukyuans suggest recent gene flow to the Okinawa Islands.

PubMed (1999)

Yoko Hatta Jun Ohashi Tadashi Imanishi Haruo Kamiyama Masaharu Iha

Polymorphism of HLA genes was investigated in a population sample of Ryukyuans living on the main island of Okinawa (n = 197), in the southwestern islands of Japan. Serological typing was applied to class I loci (HLA-A, -B, and -C) and to HLA-DRB1; nucleotide sequence-level typing was performed using PCR microtiter plate hybridization and PCR single-strand conformation polymorphism methods. Ryukyuans showed a higher frequency of DRB1*0405 and lower frequencies of DRB1*1502 and DRB1*1302 compared with Hondo Japanese living on main islands. Principal components and phylogenetic analyses of 12 East Asian populations, including Ryukyuans, were performed based on the allele frequencies of HLA-A, -B, and -DRB1. In the principal components analysis 3 Japanese populations (Ryukyuans, Hondo Japanese, and Ainu) formed a cluster and showed the highest affinity to 2 Korean populations. In the phylogenetic tree Ryukyuans and Ainu were neighbors, but the genetic distance between them was larger than the distances between Ryukyuans and Hondo Japanese and between Ryukyuans and Korean populations. The geographic cline of the predominant haplotype in Ryukyuans, A*24-B*54-DRB1*0405, suggests that an ancestral population possessing A*24-B*54-DRB1*0405 moved into the Okinawa Islands after the divergence of Ryukyuans from the Ainu. Such a recent gene flow, probably from South China to the Okinawa Islands, is considered the major cause of difference in genetic characteristics between Ryukyuans and the Ainu.

Genetic distance

Cline (biology)

Source

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Citations (33)

Announcement

Human Heredity (1988)

H. Yamaguchi T. Seno Sadaomi Egashira C.A. Green Hiroko Kaita

10.1159/000153772

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Citations (0)