Jonathan Chetrit

University of Sheffield

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Abdelhamid Benazzouz

Université de Bordeaux

Stéphanie Morin

Centre Hospitalier Universitaire de Martinique

Christian E. Gross

Université de Bordeaux

Anne Taupignon

Institut des Maladies Neurodégénératives

Rabia Bouali‐Benazzouz

Université de Bordeaux

Bernard Bioulac

Université de Bordeaux

Bérangère Ballion

Université de Poitiers

Steeve Laquitaine

École Polytechnique Fédérale de Lausanne

Mohammed A. Nassar

Tanta University

François Gonon

Institut des Maladies Neurodégénératives

Cooperative Institutions

Centre National de la Recherche Scientifique

Université de Bordeaux

Institut des Maladies Neurodégénératives

University of Sheffield

European Graduate School of Neuroscience

Maastricht University

Centre Hospitalier Universitaire de Bordeaux

Inserm

Institut de Neurosciences Cognitives et Intégratives d’Aquitaine

Centre National pour la Recherche Scientifique et Technique (CNRST)

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Intrapallidal injection of 6-hydroxydopamine induced changes in dopamine innervation and neuronal activity of globus pallidus

Neuroscience (2009)

Rabia Bouali‐Benazzouz Chun‐Hwei Tai Jonathan Chetrit Abdelhamid Benazzouz

Pars compacta

Hydroxydopamine

Apomorphine

Oxidopamine

10.1016/j.neuroscience.2009.07.034

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Mouse DRG Cell Line with Properties of Nociceptors

PLoS ONE (2015)

Ciara Doran Jonathan Chetrit Matthew C. Holley David Grundy Mohammed A. Nassar

In vitro cell lines from DRG neurons aid drug discovery because they can be used for early stage, high-throughput screens for drugs targeting pain pathways, with minimal dependence on animals. We have established a conditionally immortal DRG cell line from the Immortomouse. Using immunocytochemistry, RT-PCR and calcium microfluorimetry, we demonstrate that the cell line MED17.11 expresses markers of cells committed to the sensory neuron lineage. Within a few hours under differentiating conditions, MED17.11 cells extend processes and following seven days of differentiation, express markers of more mature DRG neurons, such as NaV1.7 and Piezo2. However, at least at this time-point, the nociceptive marker NaV1.8 is not expressed, but the cells respond to compounds known to excite nociceptors, including the TRPV1 agonist capsaicin, the purinergic receptor agonist ATP and the voltage gated sodium channel agonist, veratridine. Robust calcium transients are observed in the presence of the inflammatory mediators bradykinin, histamine and norepinephrine. MED17.11 cells have the potential to replace or reduce the use of primary DRG culture in sensory, pain and developmental research by providing a simple model to study acute nociception, neurite outgrowth and the developmental specification of DRG neurons.

Nociceptor

Veratridine

Neurite

Dorsal root ganglion

10.1371/journal.pone.0128670

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Citations (18)