Petr Barták

Palacký University Olomouc

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Petr Bednář

Palacký University Olomouc

Jana Skopalová

Palacký University Olomouc

Karel Lemr

Palacký University Olomouc

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Juraj Ševčı́k

Palacký University Olomouc

Tomáš Adam

Technical University of Košice

Zdeněk Stránský

Palacký University Olomouc

Lukáš Kučera

Palacký University Olomouc

Jitka Součková

Palacký University Olomouc

Pavla Kučerová

Palacký University Olomouc

Radek Jerga

Palacký University Olomouc

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Palacký University Olomouc

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Regional Centre of Advanced Technologies and Materials

Charles University

Czech Academy of Sciences

University Hospital Olomouc

University of South Bohemia in České Budějovice

Masaryk University

Czech Academy of Sciences, Institute of Experimental Botany

Czech Academy of Sciences, J. Heyrovský Institute of Physical Chemistry

Tomas Bata University in Zlín

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Capillary electrophoresis for detection of inherited disorders of purine and pyrimidine metabolism: A selective approach

Electrophoresis (2002)

David Friedecký Tomáš Adam Petr Barták

We developed a capillary electrophoresis method as a diagnostic tool for purine and pyrimidine metabolic disorders. Optimal conditions allowed the separation of the major diagnostic metabolites in urine samples within an analysis time of 10 min and with a separation efficiency of about 350,000 theoretical plates/m. The diagnostically important metabolites (adenine, adenosine, 2-deoxyadenosine, 2-deoxyguanosine, 2,8-dihydroxyadenine, guanosine, hypoxanthine, orotidine, orotic acid, and creatinine) were detectable at concentrations of 1.0-5.7 micromol/L. The method gives a linear calibration curve for tested purine and pyrimidine derivatives within the range of 5-500 micromol/L (r > 0.996) The coefficients of variation for the within- and between-day imprecisions were less than 3.2 and 5.8%, respectively. Characteristic abnormalities were detected in the electropherograms of urine samples from patients with purine and pyrimidine enzyme deficiencies. We provide electrophoretic and spectral characteristics of intermediates in purine and pyrimidine metabolism and possible artifacts from medication and their UV-absorbing compounds. Our method allows the detection of the majority of inborn errors of purine and pyrimidine metabolism.

Purine metabolism

Pyrimidine metabolism

Orotic acid

Deoxyguanosine

10.1002/1522-2683(200202)23:4<565::aid-elps565>3.0.co;2-s

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Citations (33)

Study of composition of espresso coffee prepared from various roast degrees of Coffea arabica L. coffee beans

Food Chemistry (2015)

Lukáš Kučera Roman Papoušek Ondřej Kurka Petr Barták Petr Bednář

Roasting

Chlorogenic Acid

Green coffee

Arabica coffee

Coffee bean

Coffea

Chemometrics

10.1016/j.foodchem.2015.12.080

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Citations (44)

DETERMINATION OF PHOSPHOLIPID FATTY ACIDS IN SEDIMENTS

Silvie Salomonová Martin Rulı́k Petr Barták

Fatty acid patterns of phospholipids were determined in different environmental samples using phospholipid fatty acids (PLFA) analysis in order to characterize their microbial community structures. Hyporheic gravel-sand sediment samples were col- lected from Sitka Stream, muddy sediment samples from several weirs on the Morava River, activated and digested sludge from Olomouc water purification plant and sand samples from filters in Olomouc water treatment plant. Profiles of PLFA indicate clear differences between these types of samples. The muddy sediments showed high percentages of ester-linked polyunsaturated fatty acids contributed by river phyto- plankton. The longer chain fatty acids (26-30 C), indicators for organic matter of

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Estimation of partition coefficients by MEKC Part 2: Anthocyanins

Journal of Separation Science (2005)

Lukáš Műller Petr Bednář Petr Barták Karel Lemr Juraj Ševčı́k

Abstract The octanol‐water partition coefficients of seven anthocyanins (in neutral form, pH = 7.0) were measured by means of MEKC. A new iterative method for the estimation of migration time of electroosmotic flow (EOF) and micelles in MEKC is presented. This calculation is based on Gauss‐Newton linearization of the dependence between migration indices and migration times of a set of suitable standards and the application of an advanced statistical evaluation procedure. The values of partition coefficients obtained with the aid of an iterative process are compared with values obtained on use of EOF and micelle markers.

10.1002/jssc.200500072

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Citations (18)

GC–MS determination of 7-dehydrocholesterol and cholesterol in milk to verify eco-friendly conditions in cow housing

Journal of Food Composition and Analysis (2023)

Jana Nádvorníková Jana Skopalová Petra Krejčí Petr Bednář Petr Barták

Heptane

Cow milk

10.1016/j.jfca.2023.105471

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Citations (1)

Liquid chromatography/high resolution tandem mass spectrometry – Tool for the study of polyphenol profile changes during micro-scale biogas digestion of grape marcs

Chemosphere (2016)

Lukáš Kučera Ondřej Kurka Petr Barták Petr Bednář

Digestate

Biogas

10.1016/j.chemosphere.2016.09.124

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Citations (10)

Determination of purine nucleoside phosphorylase activity in human erythrocytes by capillary electrophoresis

Journal of Chromatography B Biomedical Sciences and Applications (1997)

Tomáš Adam Juraj Ševčı́k Lynette D. Fairbanks Petr Barták

10.1016/s0378-4347(97)00297-1

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Citations (7)

Analysis of Markers for Characterization of Distillates

Chemické listy (2007)

M. Korhoňová R. Hejdová Petr Barták Petr Bednář Lubomı́r Čáp

Fruit distillates, such as apricot-brandy, plum vodka “Slivovitz”, cider-brandy and pear-brandy, originating from domestic production, small distilleries and manufacturing production, were analysed by gas chromatography with mass spectrometry. Less specific, but universal markers present in all types of distillates were selected for first experiments: propan-1-ol, propan-2-ol, ethyl acetate, 2-methylpropan-1-ol, 3-methylbutan-1-ol, 2-methylbutan-1-ol, 1,1-diethoxyethane, ethyl octanoate, ethyl decanoate, ethyl dodecanoate, ethyl tetradecanoate, ethyl palmitate and ethyl stearate. Quantitative data were statistically evaluated by factor analysis. Regarding the minute specificity of selected markers, unexpectedly good resolution was observed in this case. The results of factor analysis indicate a possibility of differentiation of products from different fruits. Samples of apricot-brandy and plum vodka (drupes) can be differentiated. In the same way, distillates from apples and pears (pomes) can be easily differentiated. Although, the full resolution of all samples requires discovery of other specific markers, the proposed set of low-molecular-weight compounds is a promising group of universal markers for characterisation of distillates.

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Phospholipid modified glassy carbon electrode for determination of chili peppers pungency by ex-situ extraction voltammetry

Journal of Electroanalytical Chemistry (2020)

Radek Jerga Andrea Rajcová Veronika Müllerová Petr Barták Petr Cankař

Pungency

Glassy carbon

Amperometry

10.1016/j.jelechem.2019.113790

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Citations (11)