P. Edirisinghe

University of Saskatchewan

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H. R. Kutcher

University of Saskatchewan

Helen Booker

University of Guelph

Khalid Rashid

University of Technology - Iraq

Helen M. Booker

University of Guelph

H. V. A. S. Koshila

Rasika P. Wanigatunge

University of Kelaniya

Lester Young

Genome Prairie

Sylvie Cloutier

Ottawa Research and Development Centre

R. K. S. Dias

University of Kelaniya

Lipu Wang

University of Saskatchewan

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Agriculture and Agri-Food Canada

University of Saskatchewan

Ottawa Research and Development Centre

University of Guelph

Saskatoon Medical Imaging

University of Kelaniya

Saskatoon Research and Development Centre

Saskatchewan Research Council (Canada)

Thompson Rivers University

Plant Biotechnology Institute

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Evaluation of fusarium wilt resistance in a flax germplasm

P. Edirisinghe Helen Booker H. R. Kutcher

Germ plasm

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Morphological Characterization and Molecular Identification of Three Fungal Pathogens Isolated from <em>Solanum melongena</em> L. in Sri Lanka

Journal of Agricultural Sciences – Sri Lanka (2023)

H. V. A. S. Koshila Rasika P. Wanigatunge R. K. S. Dias P. Edirisinghe

Purpose: Solanum melongena L. (brinjal/ eggplant) is a popular vegetable in Sri Lanka. Like many solanaceous crops, brinjal fruits and plants are susceptible to various diseases. The objective of this study was to isolate, confirm pathogenicity and characterize fungal pathogens causing leaf blight, yellowing and necrosis in S. melongena plants in selected localities in Gampaha district, Sri Lanka. Research Method: Fungi associated with symptomatic S. melongena plants collected from three localities in Gampaha district, Sri Lanka, were isolated onto potato dextrose agar and their pathogenicity was tested on healthy S. melongena plants. Fungal isolates that were confirmed to be pathogenic were subjected to morphological and molecular characterization. Findings: Fifteen fungal isolates were obtained from infected leaves of S. melongena plants, and four fungal isolates were confirmed to be pathogenic on S. melongena. Isolates H32a and H32b causing similar disease symptoms were identified as Lasiodiplodia theobromae with 99.81% sequence similarity by the analysis of the internal transcribed spacer region (ITS1-5.8S-ITS2). The remaining two pathogenic isolates were identified as Pseudopestalotiopsis theae and Diaporthe eugeniae, with 100% and 99.82% sequence similarities, respectively. Originality/ Value: To our knowledge, this is the first report of leaf necrosis by L. theobromae, leaf yellowing by P. theae and leaf blight by D. eugeniae associated with S. melongena plants in Sri Lanka.

Melongena

10.4038/jas.v18i2.10257

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Inheritance of Fusarium wilt resistance in flax

Canadian Journal of Plant Pathology (2023)

P. Edirisinghe Lipu Wang Lester Young Helen Booker Khalid Rashid

AbstractFusarium wilt (FW) caused by the facultative fungal saprophyte Fusarium oxysporum f. sp. lini (Fol) is a major disease affecting flax production. Cultivation of FW-resistant cultivars mitigates effects of the disease and is a requirement for variety registration. Understanding the genetic inheritance of FW resistance will facilitate the development of resistant varieties. In this study, the inheritance of wilt resistance against western Canadian isolates was studied in F8 recombinant inbred lines (RILs) of a cross between cultivars Aurore (moderately resistant) and Oliver (susceptible). Seventeen Fol isolates were collected from across western Canada and tested for differences in the severity of FW on both parental lines and two check lines under controlled environment conditions. Three of the 17 isolates that caused moderate disease severity were selected to screen the RIL population. The segregation of the RILs for FW resistance was determined under controlled conditions and in field wilt nurseries. A 3:1 ratio of susceptible to resistant RILs was observed with two F. oxysporum f. sp. lini isolates, indicating FW resistance was conditioned by two independent recessive genes. Cluster analysis of disease severity data identified two major clusters of phenotypes in the RIL population, in accordance with the 3S:1 R ratio. High heritability of FW resistance was observed at the late flowering stage, indicating a high level of genotypic variation contributing to phenotypic variation.RésuméLa fusariose, causée par le saprophyte facultatif fongique Fusarium oxysporum f. sp. lini (Fol), est une importante maladie qui nuit à la production de lin. La culture de cultivars résistants à la fusariose atténue les effets de la maladie et est une exigence quant à l'homologation des variétés. La compréhension de l'héritage génétique de la résistance à la fusariose facilitera la mise au point de variétés résistantes. Dans cette étude, l'héritage de la résistance à la flétrissure à l'égard d'isolats de l'Ouest canadien a été examiné dans des lignées endogames recombinantes F8 (LER) issues d'un croisement entre les cultivars Aurore (modérément résistant [rés.]) et Oliver (réceptif [réc.]). Dix-sept isolats de Fol ont été collectés dans tout l'Ouest canadien et testés en fonction de leurs différences relatives à la gravité de la fusariose chez les deux lignées parentales et deux lignées de validation dans des conditions contrôlées. Trois des dix-sept isolats qui ont causé une maladie de gravité modérée ont été sélectionnés pour cribler la population de LER. La ségrégation de LER en vue de la résistance à la fusariose a été déterminée dans des conditions contrôlées et dans des pépinières à fusariose en plein champ. Un rapport de 3:1 de LER réceptives à résistantes a été observé avec deux isolats de Fusarium oxysporum f. sp. lini, indiquant que la résistance à la fusariose dépendait de deux gènes récessifs indépendants. L'analyse typologique des données relatives à la gravité de la maladie a permis de reconnaître deux principaux groupes de phénotypes dans la population de LER, conformément au rapport 3 Réc.:1 Rés. L'héritabilité élevée de la résistance à la fusariose a été observée au stade avancé de la floraison, indiquant un haut degré de variation génotypique contribuant à la variation phénotypique.Keywords: flaxFusarium oxysporum f. sp. liniFusarium wiltinheritance of resistanceLinum usitatissimum L.recombinant inbred line (RIL) populationsMots clés: fusarioseFusarium oxysporum f. sp. linihéritage de la résistancelinLinum usitatissimum L.population de lignées endogames recombinantes AcknowledgmentsDr. Jean Paul Trouve and Mr. Adrian Speck of Terre De Lin, France, kindly provided the RIL population. We acknowledge the Cereal and Flax Pathology staff and the capable technical assistance provided by Mrs. S. Froese, Mr. Ken Jackle, Mrs. B. Boon, and Ms. M. Holland at the University of Saskatchewan.Disclosure statementNo potential conflict of interest was reported by the author(s).Supplementary dataSupplemental data for this article can be accessed online here: https://doi.org/10.1080/07060661.2023.2265900.Additional informationFundingThe authors gratefully acknowledge funding provided by the Saskatchewan Ministry of Agriculture, Agriculture Development Fund (ADF), project title: Characterization of Flax Germplasm for Resistance to Fusarium Wilt Caused by Fusarium oxysporum f. sp. lini. Project [#20110205]

Inbred strain

10.1080/07060661.2023.2265900

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Characterization of flax germplasm for resistance to fusarium wilt

P. Edirisinghe H. R. Kutcher Khalid Rashid Sylvie Cloutier Helen M. Booker

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