David Sánchez‐Porras

Universidad de Granada

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Fernando Campos

Universidad de Granada

Víctor Carriel

Universidad de Granada

Jesús Chato‐Astrain

Universidad de Granada

ÓD

Óscar Darío García‐García

Universidad de Granada

Miguel Alaminos

Universidad de Granada

Ingrid Garzón

Universidad de Granada

Antonìo Campos

Universidad de Granada

Miguel Etayo‐Escanilla

Universidad de Granada

Ricardo Fernández‐Valadés

Hospital Universitario Virgen de las Nieves

Cristina Blanco‐Elices

Universidad de Granada

Cooperative Institutions

Universidad de Granada

Instituto de Investigación Biosanitaria de Granada

Hospital Universitario Virgen de las Nieves

Centro de Investigación Biomédica en Red

Instituto de Salud Carlos III

Parque Tecnológico de la Salud

Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable

Junta de Andalucía

Universidad de Sevilla

Universidade Federal do Ceará

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Las mutaciones missense en el dominio ROD2 de la filamina C muestran un fenotipo con miocardiopatía restrictiva/hipertrófica y miocardio en dientes de sierra

Revista Española de Cardiología (2022)

Francisco Bermúdez-Jiménez Víctor Carriel Juan José Santos-Mateo Adrián Fernández Soledad García-Hernández

10.1016/j.recesp.2022.07.015

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Citations (1)

A Novel In Vitro Pathological Model for Studying Neural Invasion in Non-Melanoma Skin Cancer

Gels (2024)

Paula Ávila‐Fernández Miguel Etayo‐Escanilla David Sánchez‐Porras Cristina Blanco‐Elices Fernando Campos

Neural Invasion (NI) is a key pathological feature of cancer in the colonization of distant tissues, and its underlying biological mechanisms are still scarcely known. The complex interactions between nerve and tumor cells, along with the stroma, make it difficult to reproduce this pathology in effective study models, which in turn has limited the understanding of NI pathogenesis. In this study, we have designed a three-dimensional model of NI squamous cell carcinoma combining human epidermoid carcinoma cells (hECCs) with a complete peripheral nerve segment encapsulated in a fibrine-agarose hydrogel. We recreated two vital processes of NI: a pre-invasive NI model in which hECCs were seeded on the top of the nerve-enriched stroma, and an invasive NI model in which cancer cells were immersed with the nerve in the hydrogel. Histological, histochemical and immunohistochemical analyses were performed to validate the model. Results showed that the integration of fibrin-agarose advanced hydrogel with a complete nerve structure and hECCs successfully generated an environment in which tumor cells and nerve components coexisted. Moreover, this model correctly preserved components of the neural extracellular matrix as well as allowing the proliferation and migration of cells embedded in hydrogel. All these results suggest the suitability of the model for the study of the mechanisms underlaying NI.

10.3390/gels10040252

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ROD2 domain filamin C missense mutations exhibit a distinctive cardiac phenotype with restrictive/hypertrophic cardiomyopathy and saw-tooth myocardium

Revista Española de Cardiología (English Edition) (2022)

Francisco Bermúdez-Jiménez Víctor Carriel Juan José Santos-Mateo Adrián Fernández Soledad García-Hernández

Restrictive cardiomyopathy

10.1016/j.rec.2022.08.002

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Citations (11)

Basic Nerve Histology and Histological Analyses Following Peripheral Nerve Repair and Regeneration

Reference series in biomedical engineering (2021)

Jesús Chato‐Astrain Óscar Darío García‐García Fernando Campos David Sánchez‐Porras Víctor Carriel

Histology

10.1007/978-3-030-06217-0_14-1

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Citations (1)

Identification of the affective-motivational components for learning science in pharmacy students

ACTUALIDAD MEDICA (2020)

Fernando Campos David Sánchez‐Porras Jesús Chato‐Astrain Óscar Darío García‐García Cristina Blanco‐Elices

In the article the affective-motivational components for science learning in pharmacy students are identified because the learning process is conditioned by these components. The study was carried out using a specific questionnaire. The results showed variations in self-determination, self-efficacy, intrinsic motivation, and short and long-term motivation, with differences between both genders.

Identification

10.15568/am.2020.809.or03

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Citations (1)

Generation of a novel human dermal substitute functionalized with antibiotic-loaded nanostructured lipid carriers (NLCs) with antimicrobial properties for tissue engineering

Journal of Nanobiotechnology (2020)

Jesús Chato‐Astrain Isabel Chato-Astrain David Sánchez‐Porras Óscar Darío García‐García Fabiola Bermejo‐Casares

Abstract Background Treatment of patients affected by severe burns is challenging, especially due to the high risk of Pseudomonas infection. In the present work, we have generated a novel model of bioartificial human dermis substitute by tissue engineering to treat infected wounds using fibrin-agarose biomaterials functionalized with nanostructured lipid carriers (NLCs) loaded with two anti- Pseudomonas antibiotics: sodium colistimethate (SCM) and amikacin (AMK). Results Results show that the novel tissue-like substitutes have strong antibacterial effect on Pseudomonas cultures, directly proportional to the NLC concentration. Free DNA quantification, WST-1 and Caspase 7 immunohistochemical assays in the functionalized dermis substitute demonstrated that neither cell viability nor cell proliferation were affected by functionalization in most study groups. Furthermore, immunohistochemistry for PCNA and KI67 and histochemistry for collagen and proteoglycans revealed that cells proliferated and were metabolically active in the functionalized tissue with no differences with controls. When functionalized tissues were biomechanically characterized, we found that NLCs were able to improve some of the major biomechanical properties of these artificial tissues, although this strongly depended on the type and concentration of NLCs. Conclusions These results suggest that functionalization of fibrin-agarose human dermal substitutes with antibiotic-loaded NLCs is able to improve the antibacterial and biomechanical properties of these substitutes with no detectable side effects. This opens the door to future clinical use of functionalized tissues.

Viability assay

Surface Modification

Biocompatibility

10.1186/s12951-020-00732-0

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Citations (20)

Intratumoral injection of melatonin enhances tumor regression in cell line-derived and patient-derived xenografts of head and neck cancer by increasing mitochondrial oxidative stress

Biomedicine & Pharmacotherapy (2023)

Laura Martinez‐Ruiz Javier Florido César Rodríguez-Santana Alba López‐Rodríguez Ana Guerra‐Librero

Head and neck squamous cell carcinoma present a high mortality rate. Melatonin has been shown to have oncostatic effects in different types of cancers. However, inconsistent results have been reported for in vivo applications. Consequently, an alternative administration route is needed to improve bioavailability and establish the optimal dosage of melatonin for cancer treatment. On the other hand, the use of patient-derived tumor models has transformed the field of drug research because they reflect the heterogeneity of patient tumor tissues. In the present study, we explore mechanisms for increasing melatonin bioavailability in tumors and investigate its potential as an adjuvant to improve the therapeutic efficacy of cisplatin in the setting of both xenotransplanted cell lines and primary human HNSCC. We analyzed the effect of two different formulations of melatonin administered subcutaneously or intratumorally in Cal-27 and SCC-9 xenografts and in patient-derived xenografts. Melatonin effects on tumor mitochondrial metabolism was also evaluated as well as melatonin actions on tumor cell migration. In contrast to the results obtained with the subcutaneous melatonin, intratumoral injection of melatonin drastically inhibited tumor progression in HNSCC-derived xenografts, as well as in patient-derived xenografts. Interestingly, intratumoral injection of melatonin potentiated CDDP effects, decreasing Cal-27 tumor growth. We demonstrated that melatonin increases ROS production and apoptosis in tumors, targeting mitochondria. Melatonin also reduces migration capacities and metastasis markers. These results illustrate the great clinical potential of intratumoral melatonin treatment and encourage a future clinical trial in cancer patients to establish a proper clinical melatonin treatment.

Tumor progression

10.1016/j.biopha.2023.115518

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Citations (9)

Pretratamiento con hialuronidasa mejora la tinción de colágeno en notocorda de esturión

ACTUALIDAD MEDICA (2018)

Óscar Darío García‐García Jesús Chato‐Astrain A. Irastorza-Lorenzo Daniel Durand‐Herrera Francisco Campos

Objetivo: El presente estudio tiene por objetivo diseñar un método histológico para determinar la presencia y organización de la red de colágeno en la notocorda del esturión.

10.15568/am.2018.804.or03

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Citations (0)

Basic Nerve Histology and Histological Analyses Following Peripheral Nerve Repair and Regeneration

Reference series in biomedical engineering (2022)

Jesús Chato‐Astrain Óscar Darío García‐García Fernando Campos David Sánchez‐Porras Víctor Carriel

Histology

Epineurial repair

10.1007/978-3-030-21052-6_14

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Citations (1)

Improvement of Cell Culture Methods for the Successful Generation of Human Keratinocyte Primary Cell Cultures Using EGF-Loaded Nanostructured Lipid Carriers

Biomedicines (2021)

Jesús Chato‐Astrain David Sánchez‐Porras Óscar Darío García‐García Claudia Vairo María Villar-Vidal

Human skin keratinocyte primary cultures can be established from skin biopsies with culture media containing epithelial growth factor (EGF). Although current methods are efficient, optimization is required to accelerate the procedure and obtain these cultures in less time. In the present study, we evaluated the effect of novel formulations based on EGF-loaded nanostructured lipid carriers (NLC). First, biosafety of NLC containing recombinant human EGF (NLC-rhEGF) was verified in immortalized skin keratinocytes and cornea epithelial cells, and in two epithelial cancer cell lines, by quantifying free DNA released to the culture medium. Then we established primary cell cultures of human skin keratinocytes with basal culture media (BM) and BM supplemented with NLC-rhEGF, liquid EGF (L-rhEGF), or NLC alone (NLC-blank). The results showed that cells isolated by enzymatic digestion and cultured with or without a feeder layer had a similar growth rate regardless of the medium used. However, the explant technique showed higher efficiency when NLC-rhEGF culture medium was used, compared to BM, L-rhEGF, or NLC-blank. Gene expression analysis showed that NLC-rhEGF was able to increase EGFR gene expression, along with that of other genes related to cytokeratins, cell-cell junctions, and keratinocyte maturation and differentiation. In summary, these results support the use of NLC-rhEGF to improve the efficiency of explant-based methods in the efficient generation of human keratinocyte primary cell cultures for tissue engineering use.

Primary cell

Human skin

10.3390/biomedicines9111634

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Citations (9)