Silvia Martínez‐Subiela

Universidad de Murcia

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J. M. Castro Cerón

Universidad de Murcia

256

Asta Tvarijonaviciute

Universidad de Murcia

126

Fernando Tecles

Universidad de Murcia

103

Damián Escribano

Universidad de Murcia

Lorena Franco‐Martínez

Universidad de Murcia

Alberto Muñoz-Prieto

Universidad de Murcia

Marina López-Arjona

Universitat Autònoma de Barcelona

María Dolores Contreras-Aguilar

Universidad de Murcia

Camila Peres Rubio

Universidad de Murcia

Luis Pardo-Marín

Universidad de Murcia

Cooperative Institutions

Universidad de Murcia

146

Universidad Complutense de Madrid

Universitat Autònoma de Barcelona

University of Córdoba

Instituto Murciano de Investigación Biosanitaria

Consejo Superior de Investigaciones Científicas

Instituto de Salud Carlos III

University of Zagreb

University of Veterinary Medicine

Instituto de Investigación en Recursos Cinegéticos

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Effects of Orchidectomy in Selective Biochemical Analytes in Beagle Dogs

Reproduction in Domestic Animals (2011)

Asta Tvarijonaviciute Silvia Martínez‐Subiela JD Carrillo‐Sanchez Fernando Tecles J. M. Castro Cerón

Contents The objective of this study was to evaluate the possible effects of orchidectomy and associate hormonal changes on circulating concentrations of acute‐phase proteins (APPs) (CRP – C‐reactive protein; Hp – haptoglobin; Cp – ceruloplasmin), adiponectin and IGF‐1 in dogs. For this, a total of five adult Beagle dogs were subjected to orchidectomy. Blood samples were taken before neutering, during six consecutive days and on weeks 2, 4, 8 and 12 after surgery. Appropriate diet regime was maintained to keep stable body weight of the dogs. Concentrations of APPs significantly increased on days 2–3 for CRP and 2–7 for Hp and Cp. On days 3–4 after neutering, adiponectin levels were significantly lower than before surgery (p < 0.05 and <0.01, respectively). After this initial change, adiponectin did not show any significant alteration during the 3 months. Serum IGF‐1 concentrations were significantly decreased at days 2–5 after neutering. In addition, on weeks 8 and 12 serum IGF‐1 levels were significantly lower (p < 0.001 and <0.01 respectively) in comparison with those before surgery. In conclusion, orchidectomy induced a short‐term inflammatory process that was associated with the increase in serum levels of APPs and decrease in IGF‐1 and adiponectin levels. However, orchidectomy did not result in long‐term changes of circulating concentrations of APPs or adiponectin. Although a decrease in IGF‐1 levels was recorded 2 months after surgery, possibly as a consequence of associated decrease in androgen levels or food restriction.

Neutering

Beagle

Ceruloplasmin

Haptoglobin

10.1111/j.1439-0531.2011.01765.x

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Citations (17)

Changes in Serum Proteins in Cats with Obesity: A Proteomic Approach

Animals (2025)

Esmeralda Cañadas-Vidal Alberto Muñoz-Prieto Dina Rešetar Maslov Ivana Rubić Juan Carlos González‐Sánchez

Obesity is defined as the excessive accumulation of adipose tissue and is currently the most common disease in cats. Similarly to humans, obesity negatively impacts the health and welfare of cats, predisposing them to many other disorders. The objective of this study was to compare the serum proteomes of normal-weight and overweight/obese cats, aiming to gain insights into the physiopathology of feline obesity and potentially identify new biomarkers. For this, serum samples from a total of 20 adult neutered domestic shorthair client-owned cats, ten normal weight and ten overweight/obese, were submitted to tandem mass tags labelling and liquid chromatography-mass spectrometry (LC-MS/MS) analysis. A total of 288 proteins were detected in the serum samples. Out of these, 12 proteins showed statistically significant differences in abundance between control cats and cats with obesity, namely Ig-like domain-containing protein, Alpha-2-HS-glycoprotein, Complement C8 gamma chain, An-tithrombin-III, Serpin family A member 1, Complement factor H, C3-beta-c, Albumin, C4b-binding protein alpha chain, Alpha-1-B glycoprotein, Solute carrier family 12 member 4, and Fibronectin. Overall this report identifies new proteins involved and provides additional knowledge about the physiopathological changes related to feline obesity.

Tandem mass tag

Proteome

10.3390/ani15010091

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Citations (0)

S-100 Proteins: Basics and Applications as Biomarkers in Animals with Special Focus on Calgranulins (S100A8, A9, and A12)

Biology (2023)

J. M. Castro Cerón Alba Ortín-Bustillo María José López-Martínez Silvia Martínez‐Subiela P.D. Eckersall

S100 proteins are a group of calcium-binding proteins which received this name because of their solubility in a 100% saturated solution of ammonium sulphate. They have a similar molecular mass of 10–12 KDa and share 25–65% similarity in their amino acid sequence. They are expressed in many tissues, and to date 25 different types of S100 proteins have been identified. This review aims to provide updated information about S100 proteins and their use as biomarkers in veterinary science, with special emphasis on the family of calgranulins that includes S100A8 (calgranulin A; myeloid-related protein 8, MRP8), S100A9 (calgranulin B; MRP14), and S100A12 (calgranulin C). The proteins SA100A8 and S100A9 can be linked, forming a heterodimer which is known as calprotectin. Calgranulins are related to the activation of inflammation and the immune system and increase in gastrointestinal diseases, inflammation and sepsis, immunomediated diseases, and obesity and endocrine disorders in different animal species. This review reflects the current knowledge about calgranulins in veterinary science, which should increase in the future to clarify their role in different diseases and potential as biomarkers and therapeutic targets, as well as the practical use of their measurement in non-invasive samples such as saliva or feces.

S100A9

S100A8

Proteome

Proteogenomics

10.3390/biology12060881

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Citations (12)

Changes in lactate, ferritin, and uric acid in saliva after repeated explosive effort sequences

The Journal of Sports Medicine and Physical Fitness (2019)

Lorena Franco‐Martínez Asta Tvarijonaviciute Silvia Martínez‐Subiela Gonzalo Márquez Néstor Martínez Díaz

The aim of the present study was to evaluate the changes in a panel of biomarkers including lactate, ferritin, and uric acid in saliva of untrained and trained subjects after repeated explosive effort sequences, and to analyze the differences in interpretation of these biomarkers depending on the way the data is expressed (without any correction or corrected by protein content or flow).Eighteen volunteers (11 untrained and 7 trained) performed 8 consecutive explosive effort sequences (60-meters sprints). Blood and saliva samples were collected before and after each sequence. Salivary data was analyzed as absolute concentration and after correcting by their protein content and flow.Lactate in saliva showed increases with acute exercise, being these increases of higher magnitude in non-trained subjects. In addition, when corrected by total protein, lactate in saliva correlates with blood lactate in non-trained subjects. Ferritin and uric acid in saliva did not show changes after the effort sequences.From the biomarkers evaluated, the measurement of lactate in saliva corrected by total protein could be useful for the assessment of fatigue induced during repeated explosive effort sequences and could be potentially used as a non-invasive alternative to blood lactate. This report also points out that way of reporting the saliva analytes could greatly influence the interpretation of the results and that lactate in saliva has a different dynamic in trained and non-trained individuals that should be further explored.

Repeated measures design

10.23736/s0022-4707.18.08792-3

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Citations (13)

Fast measurement of serum amyloid A in different specimens from swine by using a new one-step time-resolved fluorescent immunoassay

Journal of Veterinary Diagnostic Investigation (2011)

Laura Soler A.M. Gutiérrez Silvia Martínez‐Subiela J. M. Castro Cerón

The objective of the present study was to develop a one-step, fast, competitive time-resolved fluorescent immunoassay to determine porcine serum amyloid A (SAA) by using species-specific reagents. The assay consisted of an all-in-one format involving only 55 min of incubation that was adapted and validated for use in 3 different specimens: serum, saliva, and meat juice. The method had overall within- and between-run coefficients of variation under 8% and 12%, respectively, and coefficients of determination higher than 0.93 for linearity under dilution analysis for all specimens. The limits of detection were 0.32 mg/l, 0.28 mg/l, and 1.74 mg/l for serum, saliva, and meat juice measurements, respectively. Upper and lower limits of quantification were determined for each sample type and resulted in wide assay ranges that allowed a precise SAA measurement in all the fluids investigated. Statistically significant differences (P = 0.0004 for serum and P < 0.0001 for the saliva and meat juice samples) in SAA levels were found when healthy (n = 20) and diseased (n = 20) pigs were compared. The obtained results indicate that this fast, sensitive, and robust assay for SAA measurement could be of use to determine health and welfare status in swine by employing alternative samples to serum.

Coefficient of variation

Dilution

Incubation period

10.1177/1040638711416623

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Citations (12)

Urinary clusterin as a renal marker in dogs

Journal of Veterinary Diagnostic Investigation (2012)

J.D. García-Martínez Asta Tvarijonaviciute J. M. Castro Cerón Marco Caldín Silvia Martínez‐Subiela

A validation of a species-specific enzyme immunoassay for urinary clusterin measurement in dogs was performed, and the use of urinary clusterin as a marker of renal damage was evaluated in a population of dogs with leishmaniasis. Urine was obtained from 75 dogs; 64 dogs had leishmaniasis and 11 were healthy. The dogs with leishmanias were divided into 5 groups: I ( n = 9; serum creatinine [SCr] < 1.4 mg/dl, urinary protein-to-creatinine [UPC] ratio ≤ 0.5); II ( n = 29; SCr < 1.4 mg/dl, UPC > 0.5); III ( n = 6; SCr ≥ 1.4 mg/dl to <2 mg/dl, UPC > 0.5); IV ( n = 13; SCr ≥ 2 mg/dl to <5 mg/dl, UPC > 0.5); and V ( n = 7; SCr ≥ 5 mg/dl, UPC > 0.5). The urinary clusterin concentration was measured, and the urinary clusterin-to-creatinine ratio was calculated. Canine urinary clusterin assay showed good analytical performance based on precision accuracy and limit-of-detection results. There was a statistically significant increase in urinary clusterin and clusterin-to-creatinine ratio in groups II–V compared with group I and healthy group. The results of the current study showed that urinary clusterin concentration and urinary clusterin-to-creatinine ratios are increased in dogs with analytical evidences of renal damage and that the urinary clusterin-to-creatinine ratio might be used as a potential early biomarker of chronic kidney disease.

Clusterin

10.1177/1040638711435112

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Citations (33)

Changes in salivary analytes in canine parvovirus: A high-resolution quantitative proteomic study

Comparative Immunology Microbiology and Infectious Diseases (2018)

Lorena Franco‐Martínez Asta Tvarijonaviciute Anita Horvatić Nicolas Guillemin J. M. Castro Cerón

Proteome

Canine parvovirus

10.1016/j.cimid.2018.09.011

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Citations (21)

Changes in saliva of dogs with canine leishmaniosis: A proteomic approach

Veterinary Parasitology (2019)

Lorena Franco‐Martínez Asta Tvarijonaviciute Anita Horvatić Nicolas Guillemin Luis J. Bernal

Proteome

Interactome

Salivary Proteins

Human proteins

10.1016/j.vetpar.2019.06.014

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Citations (21)

Revista Electrónica en Educación y Pedagogía (2017)

Análisis Cualitativo Myriam Helena Tobón Pinto Silvia Martínez‐Subiela Miguel López

Resumen: El presente trabajo se centra en el estudio de las circunstancias que determinan las bajas de asistencia de niños entre los 4 y los 9 años de edad a las actividades que se ofertan en los Museos de Arte y Numismática del Banco de la República en Bogotá, Colombia.A partir de la metodología cualitativa, y bajo miradas que apuntan a dinámicas de participación social como la animación sociocultural, postulados del paradigma constructivista en relación a la educación en museos y teorías sobre la construcción identitaria desde el espacio museal, esta investigación plantea recoger las expectativas tanto de profesionales de los museos como de docentes, niños y familias vinculados a Casas Comunitarias vecinas a los espacios culturales.Los resultados de la investigación permiten dar luces sobre las estrategias de acción que deben darse al interior de la comunidad, y reflexionan sobre los componentes pedagógico, social y patrimonial en el escenario de la actividad cultural, proponiendo un modelo de comprensión global del fenómeno.

10.15658/rev.electron.educ.pedagog17.09010102

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Citations (1)

Longitudinal analysis of acute-phase proteins in saliva in pig farms with different health status

animal (2011)

A.M. Gutiérrez J. M. Castro Cerón Pablo Cerezuela A. Montes Silvia Martínez‐Subiela

This study assesses the utility of saliva samples to monitor the time course of the acute-phase response to different viruses in pigs under field conditions by using time-resolved immunofluorometric assays (TR-IFMA). A total of 30 pigs from three different farms, located in Southeast Spain, were used. Farm 1 had outbreaks of porcine circovirus type 2, farm 2 had infections with porcine reproductive and respiratory syndrome virus and farm 3 had concomitant infections with both viruses. Serology was used to determine the time of seroconversion of pigs to two different pathogens. The levels of two acute-phase proteins (APPs), C-reactive protein (CRP) and haptoglobin (Hp), were measured in saliva and serum samples and compared with pig's serology. Kinetic curves of both APPs across the study obtained in saliva samples were similar to those of serum, with R of 0.68 and 0.78 for CRP and Hp, respectively. The median CRP and Hp concentrations in saliva were higher around the theorized time of infection, according to previous experimental studies, and at seroconversion of animals. CRP increments were apparent 1 week before the increments obtained in Hp. These findings indicate that salivary APP concentrations, by using TR-IFMA, can be used in longitudinal studies as non-invasive early indicators of health status.

Seroconversion

Haptoglobin

Longitudinal Study

10.1017/s1751731111001662

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Citations (29)