Pei‐Ru Jheng

Cold atmospheric plasma (CAP) is a promising tool to overcome certain cancerous and precancerous conditions in dermatology. A scheme of transferred CAP was first developed to treat melanoma (B16F10) skin cancer cells as well as non-malignant (L929) cells in vitro. CAP was transferred using a silicone tube with a jet system that was developed and was assessed as to whether it could generate reactive oxygen and nitrogen species (RONS) at near-room temperature. The transferred CAP was characterized electrically and spectroscopically. Biological data showed that the transferred CAP killed cancer cells but not non-malignant (L929) cells. Plasma treatment was effective with a time duration of 30 s, whereas non-malignant (L929) cells were less damaged during plasma treatment. In addition, catalase (CAT) enzyme was applied to neutralize and detoxify the RONS generated by the transferred CAP. These findings suggest that transferred CAP can be considered a melanoma cancer therapy.

10.3390/app11136181

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Citations (8)

Near-infrared-driven photoablation of lung cancer tumors utilizing biomimetic platelet-polyethyleneimine-polypyrrole drug-free nanoparticles

Materials & Design (2022)

Thierry Burnouf Pei‐Ru Jheng Yun-Hsuan Chen Lekshmi Rethi Lekha Rethi

Platelets have unique properties that can be used in a variety of clinical settings. Their ability to target and accumulate in the lung cancer microenvironment can be exploited as a therapeutic targeted delivery method. In this study, we developed a drug-free biomimetic platelet-polyethyleneimine polypyrrole (PLT-PEI-PPY) conjugate that expresses epidermal growth factor (EGF) as a novel receptor-mediated material for efficient photothermal near-infrared (NIR) irradiation-hyperthermia and lung tumor ablation therapy. A neutral charge, photothermal response, and serum stability were discovered to be unique physiochemical features of this material. The PLT-PEI-PPY nanoparticles selectively aggregated in lung cancer cells in vitro and in vivo and had an anticancer impact via EGF receptor-mediated cancer cell endocytosis. In vivo, systemic injection of the platelet-PPY nanomaterial into mice was found to be safe and could fool the innate macrophage recognition system allowing to extend the residence time, and to improve the distribution to lung tumor tissues and the photothermal therapeutic efficacy under NIR illumination. By attaching photothermally responsive PEI-PPY nanoparticles to platelet membranes and exposing them to NIR irradiation, targeted ablation of lung malignancies was made possible. This nanoformulation, free of hazardous anticancer drugs, demonstrated a safer and more-effective bio-inspired photothermal-driven ablation therapy for lung cancer.

Polypyrrole

10.1016/j.matdes.2022.110481

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Citations (12)

Self-adaptable calcium-based bioactive phosphosilicate-infused gelatin-hyaluronic hydrogel for orthopedic regeneration

International Journal of Biological Macromolecules (2023)

Lekha Rethi Chin-Chean Wong W Liu Chieh‐Ying Chen Pei‐Ru Jheng

Gelatin

Biocompatibility

Regenerative Medicine

Bioactive Glass

Biomaterial