Shaginian Ia

Gamalei Institute of Epidemiology and Microbiology

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Gintsburg Al

Sechenov University

Vertiev IuV

Gamalei Institute of Epidemiology and Microbiology

Stepanova Mv

Chernukha MIu

Romanova IuM

Gamalei Institute of Epidemiology and Microbiology

Dmitrienko Oa

Zueva Vs

Otman Ls

Ezepchuk IuV

Ferapontov Gk

Cooperative Institutions

Gamalei Institute of Epidemiology and Microbiology

Ministry of Health of the Russian Federation

Russian Academy of Sciences

Institute of Biomedical Problems

University of Minnesota

Research Centre for Medical Genetics

National Research Centre

Central Research Institute of Epidemiology

National Research Institute for Veterinary Virology and Microbiology of Russia

Nizhniy Novgorod Research Institute of Epidemiology and Microbiology named after Academician I.N. Blokhina

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

[Methods for detecting drug-resistant staphylococci in the human body under natural conditions].

PubMed (1975)

Zueva Vs Shaginian Ia Shelenkova Ln Otman Ls

Two methods were compared for determination of drug resistant staphylococci on the nasal mucosa of patients, i. e. the routine method for determination of staphylococcal sensitivity to antibiotics and the method of direct plating out of the starting material onto agarized media containing antibiotics. Staphylococci resistant to streptomycin, tetracycline, chloramphenicol, erythromycin, monomycin, axacillin and less frequent to penicillin were found more often with the 2nd method. A method of proportions was developed for testing sensitivity of staphylococci in purulent inflammatory foci of the patients. It provided characterization of the staphylococcal population from the foci by the number of the antibiotic resistant microbial cells.

Source

Cite

Citations (0)

[Genetic mapping of the regulatory gene determining the synthesis of enterotoxin in Vibrio cholerae].

PubMed (1986)

Н. И. Смирнова Livanova Lf Shaginian Ia Motin Vl

The data of genetic mapping of the cholera toxin regulatory gene by conjugation mating of Vibrio cholerae eltor donor strain with V. cholerae classica recipients are presented. The close genetic linkage of tox locus to pur-63 is shown. The gene order asp - cys - nal - pur-61 - trp - his - pur-63 - tox - ile of the chromosomal region examined is established.

Cholera toxin

Cholera

Source

Cite

Citations (0)

[PCR genetic typing of pathogenic microorganisms].

PubMed (1995)

Shaginian Ia Gintsburg Al

Basic principles, characteristics, methodical peculiarities, and strategy of choice of PCR-mediated methods of genetic typing are discussed. Existing and potential fields of application of these methods for tackling many unsolved problems of molecular epidemiology of bacterial infections are considered. Advantages of PCR-mediated methods of genetic typing compared with other techniques used for studying genomic polymorphism are demonstrated. Possibilities for developing novel PCR-mediated methods with the aim of improving genetic typing are analyzed.

Source

Cite

Citations (0)

[Molecular epidemiology of infections caused by methicillin-resistant staphylococci].

PubMed (2003)

Shaginian Ia Dmitrienko Oa

The review deals with the periodicity of the spread of methicillin-resistant S. aureus (MRSA) strains during the last 40 years, the mechanism of their resistance to methicillin and other beta-lactamic antibiotics, the genetic control of methicillin resistance, the genome organization of mec DNA and its possible cause, as well as the organization of epidemiological surveillance on MSRA in hospitals. The problem of changes in the epidemiology of staphylococcal infections due to the appearance of MRSA in the absence of contacts with carriers, treatment with antibiotics or stay in a hospital is discussed. The concern of public health authorities in connection with the emergence of MRSA strains, moderately resistant or resistant to vancomycin, is also discussed. The most promising programs of the MRSA study, as well as the optimum programs introduced in economically developed counties for the control of hospital infections caused by MRSA, are considered.

Molecular Epidemiology

MSRA

Source

Cite

Citations (1)

[Determination of cholera vibrion enterotoxin by an aggregate hemagglutination method].

PubMed (1979)

Baroian Ov Gaĭlonskaia In Ferapontov Gk Shaginian Ia Vertiev IuV

Cholera

Source

Cite

Citations (0)

[The toxin-producing capacity of populations of Vibrio cholerae of different origins].

PubMed (1990)

Osaulenko Ov Maramovich As Veĭde Aa Vertiev IuV Shaginian Ia

As the result of the study of toxin formation in 165 V. eltor strains having different virulence, most of the virulent cultures have shown stable toxin production, though 5-10% of their colonies have proved to be nontoxigenic. In faintly virulent strains toxin production was found to be unstable, which is indicative of the heterogeneity of their population composition as regards their capacity for toxin formation. The population of avirulent strains consists mainly of nontoxigenic clones (95.7%).

Cholera toxin

Source

Cite

Citations (0)

[The role of "quorum sensing" regulation system in symbiotic interaction of bacteria Burkholderia cepacia and Pseudomonas aeruginosa during mixed infection].

Zhurnal mikrobiologii epidemiologii i immunobiologii (2006)

Chernukha MIu Shaginian Ia Romanova IuM Maleev Gv Gintsburg Al

Data concerning mechanisms of strengthening virulence of bacteria B.cepacia and P.aeruginosa during experimental mixed-infection are submitted. Results of in vitro studies of acylhomoserine lactones (AHL) with various length of a carbon lateral circuit have shown that butanoyl-homoser-ine lactone (C4), hexanoyl-homoserine lactone (C6) and octanoyl-homoserine lactone (C8) separately and in various combinations display different effects on growth of bacteria, formation of colonies and production of pathogenicity factors. Experiments on animals have shown strengthening virulence of bacteria B.cepacia by C4, C6 and C8 lactones when introduced simultaneously. Strengthening of virulence of the P.aeruginosa strain under the action of lactone C6 and simultaneous action of C4, C6 and C8 lactones was observed. The data obtained give grounds for assumption, that bacteria may use exogenous lactones, including those produced by closely related bacteria, during their life cycle.

Homoserine

Source

Cite

Citations (2)

[Trial of different nutrient media for the production of an intracellular thermolabile enterotoxin by Escherichia coli strains H74-114 and 86].

PubMed (1985)

Stepanova Mv Shaginian Ia Noskova Vp Vertiev IuV Ezepchuk Ev

The conditions of cultivation, ensuring the maximum accumulation of intracellular thermolabile enterotoxin in the cultures of two E. coli strains of different origin, have been studied. Culture media manufactured in the USSR have been selected and the conditions of cultivation, necessary for obtaining intracellular thermolabile enterotoxin in preparative amounts, have been established. Under these conditions the yield of thermolabile enterotoxin in 1.4 mg per 1 liter of culture medium for strain H74-114 and 1.0 mg per 1 liter of culture medium for strain 86.

Thermolabile

Strain (injury)

Source

Cite

Citations (0)

[Modification of a method of passive immune hemolysis on a solid medium for detecting the production of thermolabile enterotoxins by Vibrio cholerae and Escherichia coli strains].

PubMed (1983)

Shaginian Ia Marakusha Bi

A modification of the passive immune hemolysis method for the determination of the production of thermolabile enterotoxins by V. cholerae and E. coli is proposed. This modification permits the use of solid culture media. Experiments with cholera enterotoxin have demonstrated that the sensitivity of the modified method is 8-10 times higher than that of the Elek method. Similar results have been obtained with the use of the proposed method in the study of the capacity of different V. cholerae and E. coli strains for producing enterotoxins. The results obtained with the use of this method have been found to correlate with those obtained by means of the skin test and passive immune hemolysis in a liquid medium. We have used the modified method in the study of the production of thermolabile enterotoxin in transconjugants obtained by the hybridization of E. coli strain GA 107 carrying plasmid pCG86 which determines the synthesis of thermolabile and thermostable enterotoxins and E. coli strain K12 C600 R. The results obtained in the study of toxin formation in 99 transconjugants, carried out with the use of the proposed method, the skin test and passive immune hemolysis, have been shown to coincide.

Thermolabile

Strain (injury)

Source

Cite

Citations (0)

[Virulent properties of hospital strains of bacteria of the Burkholderia cepacia complex, isolated in hospitals of Moscow].

PubMed (2006)

Chernukha MIu Alekseeva Gv Shaginian Ia Romanova IuM Stepanova Tv

The results of the study of hospital strains of the B. cepacia complex, isolated in hospitals of Moscow, with the use of phenotypical and molecular-genetic methods are presented. The phenotypical methods made it possible to differentiate Russian strains and classify them with a group of genomovars (I, III, IV). As the result the epidemic importance of the strains with epidemic markers, having specific characteristics for every clinic, was determined. The detection of the collection of genes cepI and cepR in the strains made confirmed the epidemic importance of the stains which had, due to the regulatory "quorum sensing" (QS) system, the potential capacity for inducing infection and persisting in the patient's body. The presence of gene cepR in all strains and the absence of gene cepl in 33% of strains gave evidence to suggest that in some strains the activation of the production of pathogenicity factors required the presence of other bacteria having the fully developed QS system. Thus, the new complex approach with the use of phenotypical and molecular-genetic methods permits more precise identification of the source of hospital infection induced by the bacteria of the B. cepacia complex.

Burkholderia cepacia complex

Source

Cite

Citations (1)