Béatrice Descamps‐Latscha

Hôpital Necker-Enfants Malades

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Véronique Witko‐Sarsat

Inserm

Tilman B. Drüeke

Inserm

Anh Thu Nguyen

Assistance Publique Hôpitaux de Marseille

Thao Nguyen‐Khoa

Hôpital Necker-Enfants Malades

Ziad A. Massy

Hôpital Ambroise-Paré

André Herbelin

Centre Hospitalier Universitaire de Poitiers

Paul Jungers

Hôpital Necker-Enfants Malades

Flora Zavala

University of Piura

Pablo Ureña

Université Paris Cité

J. Zingraff

Hôpital Necker-Enfants Malades

Cooperative Institutions

Inserm

152

Hôpital Necker-Enfants Malades

Université Paris Cité

Centre National de la Recherche Scientifique

Assistance Publique – Hôpitaux de Paris

Sorbonne Université

Délégation Paris 5

Sorbonne Paris Cité

Institut Necker Enfants Malades

Hôpital Saint-Louis

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Disturbed myeloperoxidase-dependent activity of neutrophils in cystic fibrosis homozygotes and heterozygotes, and its correction by amiloride.

The Journal of Immunology (1996)

Véronique Witko‐Sarsat Robert C. Allen Marc Paulais Thu Anh Nguyen Gilles Bessou

The present study addresses the question of a possible linkage between the cystic fibrosis (CF) genetic autosomal recessive disorder and disturbance in neutrophil function. Neutrophil-dominated chronic airway inflammation is present at an early age in children with CF, even in the absence of detectable infection. As evidenced by extracellular superoxide anion release (measured by lucigenin luminescence) or intracellular hydrogen peroxide production (measured by 2',7'-dichlorofluorescein (DCF) fluorescence), no significant difference in the nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase activity of isolated neutrophils was observed in noninfected CF children (homozygotes), their mothers or fathers (CF heterozygotes), and controls. In contrast, both myeloperoxidase (MPO)-dependent oxygenation activity (measured by luminol luminescence) and chloramine release were increased significantly in both CF homozygotes and heterozygotes as compared with controls. In the presence of either amiloride (a sodium channel inhibitor and sodium/proton antiport blocker) or EIPA (5-ethyl-N-isopropyl-amiloride, a specific inhibitor of the antiport), or choline buffer, intracellular MPO activity was decreased significantly in controls and in the CF homozygotes and heterozygotes, thus bringing intracellular MPO-dependent activity in CF subjects back to the level of controls. Extracellular release of MPO, measured by an ELISA to provide an activity-independent assessment of the enzyme, was increased only in CF homozygotes, and was decreased by amiloride and choline buffer, but not by EIPA. We conclude that a modification of intracellular pH and/or ionic concentrations may be related to the altered MPO enzymatic activity observed in CF neutrophils.

Amiloride

Intracellular pH

Neutrophil elastase

10.4049/jimmunol.157.6.2728

Cite

Citations (102)

Biochemical and spectrophotometric significance of advanced oxidized protein products

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease (2004)

Chantal Capeillère‐Blandin Valérie Gausson Béatrice Descamps‐Latscha Véronique Witko‐Sarsat

Hypochlorous acid

Nitrotyrosine

Absorbance

Serum Albumin

10.1016/j.bbadis.2004.02.008

Cite

Citations (197)

Predictive Value of Whole Blood Chemiluminescence in Patients With Alcoholic Hepatitis

Hepatology (1990)

F. Lunel Béatrice Descamps‐Latscha Diane Descamps Y. Le Charpentier P. Grippon

Recent reports suggest that ethanol metabolism leads to reactive oxygen intermediates that may be responsible for the lesions observed in alcoholic hepatitis. This study investigated the production of reactive oxygen intermediates in peripheral blood phagocytes of patients with alcoholic hepatitis and attempts to evaluate its predictive value. Using a luminol-dependent chemiluminescence method, reactive oxygen intermediate production was measured directly within microamounts of whole blood, both in the absence (basal chemiluminescence production) and in the presence of phagocyte-stimulating agents including latex, zymosan, phorbol myristate acetate and N-formyl-methionyl-leucyl-phenylalanine. Thirty patients with well-documented and histologically proven alcoholic hepatitis were studied. Pugh's and Child's classification, Orrego's composite clinical and laboratory index and Maddrey's discriminant function were used to assess the prognosis of the liver disease. Patients were followed up monthly for 6 mo. Results were compared with those obtained in 17 patients with nonalcoholic liver disease and in 78 normal control subjects. Basal chemiluminescence production was significantly higher in patients with alcoholic hepatitis than in those with nonalcoholic liver disease and in normal subjects (p less than 0.001). Chemiluminescence responses to latex, zymosan and phorbol myristate acetate were significantly lower in alcoholic hepatitis patients than in normal subjects (p less than 0.001); however, when compared with nonalcoholic liver disease patients, these responses were significantly decreased only in the presence of zymosan (p less than 0.05). Both basal chemiluminescence production (p less than 0.001) and zymosan-induced chemiluminescence responses (p less than 0.02) were closely related to alcoholic hepatitis prognosis indices (i.e., Pugh's and Child's classification, Orrego's composite clinical and laboratory index and Maddrey's discriminant function.(ABSTRACT TRUNCATED AT 250 WORDS)

Zymosan

Alcoholic Hepatitis

Liver disease

Basal (medicine)

10.1002/hep.1840120213

Cite

Citations (13)

Effect of LPS on the oxidative metabolism of peritoneal and spleen cells from LPS sensitive and resistant mice.

PubMed (1984)

M.N. Feuillet-Fieux Rachel Golub Anh Thu Nguyen P. Zamfirescu Béatrice Descamps‐Latscha

The influence of LPS on peritoneal and spleen cell oxidative metabolism was investigated in LPS sensitive (C57BL/6) and LPS resistant (C3H/HeJ) mice following intraperitoneal or subcutaneous injection, by measurement of chemiluminescent (CL) responses to latex particles. In C57BL/6 mice, LPS induced a marked increase in peritoneal and spleen cell CL responses, regardless of the route of injection. On the 2nd day, the effect of LPS on peritoneal cells could be fully explained by an inflammatory reaction, while on the 4th day it could be related to an "activated state" of peritoneal macrophages. In contrast, such an in vivo effect of LPS in peritoneal or spleen cell CL responses was not found in C3H/HeJ mice except on the 2nd day following the injection and with the highest LPS dosage and could be totally due to the LPS induced inflammatory reaction. In vitro studies showed that extremely low concentration of LPS increased CL response capacities of C57BL/6 spleen cell to latex particles. In contrast, CL responses from C3H/HeJ spleen cells remained unaffected by LPS except when submitted to concentrations which proved toxic for the sensitive strain CL producing cells. These results lend additional evidence for the involvement of reactive oxygen species (ROS) in the metabolic consequences of LPS-induced macrophage activation.

Peritoneal cavity

Intraperitoneal injection

Source

Cite

Citations (3)

Development of cytochrome b₅₅₈ and oxidative metabolism in human granulocytes, monocytes and during differentiation of HL‐60 and U 937 cells

Biology of the Cell (1990)

Chantal Capeillère‐Blandin Geneviève Chauvet Françoise Tresset Béatrice Descamps‐Latscha

Summary— The development of cytochrome b 558 Cyt b) as determined spectrophotometrically, was investigated in human polymorphonuclear neutrophils (PMN), monocytes (MN) and during differentiation of HL‐60 and U 937 cells induced by retinoic acid (RA) alone or in combination with IFNγ. O 2 − release in response to a panel of stimulating agents, ie latex particles, opsonised zymosan, PMA, Con A and fMLP, was monitored by lucigenin‐amplified chemiluminescence (CL). In parallel the expression of myeloperoxidase (MPO) was investigated and its catalytic activity on H 2 O 2 related to luminol‐amplified CL responses. In mature PMN and MN phagocytes, regardless of the stimulating agent, the O 2 − production is closely related to Cyt b but not to MPO specific contents. In differentiated HL‐60 and U 937 cells, the oxidative metabolism increases in parallel with Cyt b specific contents, both being enhanced by the addition of IFNγ to the RA treatment. However, marked differences in the O 2 − production intensities are observed depending on the stimulating agent tested and the state of differentiation considered. The PMA‐stimulated O 2 − production is rather low ie 100 and 20 times less in granulocytic HL‐60 and monocyto‐macrophagic U 937 cells than in PMN and MN respectively. Latex, zymosan and Con A stimulated responses are close to those of MN, in monocyte‐macrophagic U 937 cells. In conclusion, these data show that during differentiation; 1), Cyt b plays a critical role in O 2 − production; 2), the pathways leading to NADPH oxidase activation are diversely modulated following phagocyte differentiation with IFNγ and/or with RA.

Zymosan

Monocyte

10.1111/j.1768-322x.1990.tb00001.x

Cite

Citations (8)

Dialysis‐Induced Oxidative Stress: Biological Aspects, Clinical Consequences, and Therapy

Seminars in Dialysis (2001)

Béatrice Descamps‐Latscha Tilman B. Drüeke Véronique Witko‐Sarsat

Oxidative stress, which results from a rupture in the natural balance between pro- and antioxidant systems, is considered as a major factor in dialysis-associated morbidity and mortality. Emerging pharmacologic and dialytic antioxidant therapeutic and dialysis strategies should enable us to reduce the harmful consequences of oxidative stress in dialysis patients. Moreover, since there is increasing evidence of oxidative stress long before the initiation of maintenance dialysis, antioxidant therapeutic strategies should probably be developed very early in the course of renal failure.

Dialysis Therapy

10.1046/j.1525-139x.2001.00052.x

Cite

Citations (162)

Homocyst(e)ine, oxidative stress, and endothelium function in uremic patients

Kidney International (2001)

Ziad A. Massy I. Ceballos Bernadette Chadefaux-Vekemens Thao Nguyen‐Khoa Béatrice Descamps‐Latscha

10.1046/j.1523-1755.59.s78.54.x

Cite

Citations (0)

Progression rate to end-stage renal failure in non-diabetic kidney diseases: a multivariate analysis of determinant factors

Nephrology Dialysis Transplantation (1995)

Paul Jungers T Hannedouche Yukihiro Itakura G Albouze Béatrice Descamps‐Latscha

The respective contribution of the type of nephropathy, gender, and proteinuria, and of the potentially alterable factors blood pressure level and daily protein intake on the rate of progression in non-diabetic renal diseases is debated.We retrospectively analysed the influence of primary renal disease, gender, urinary protein excretion, mean arterial pressure (MAP), and dietary protein intake on the rate of decline in creatinine clearance (delta Ccr) in 159 adult patients with well-defined nondiabetic kidney diseases. All patients had been followed from a baseline Crr of 40-50 ml/min/1.73 m2 until endstage renal disease and need for dialysis.Mean (+/- SD) delta Ccr (ml/min/1.73 m2/year) was 9.9 +/- 6.5 in 51 patients (45 males) with chronic glomerulonephritis, 6 +/- 2.5 in 50 patients (26 males) with polycystic kidney disease, 5.5 +/- 2.4 in 17 patients (16 males) with hypertensive angionephrosclerosis, and 3.9 +/- 2 in 41 patients (21 males) with chronic tubulointerstitial nephritis. delta Ccr was higher in males than in females (7.5 +/- 5.2 versus 4.8 +/- 2.5; P < 0.001). Linear regression analysis of the whole population disclosed a strong relationship between delta Ccr and proteinuria (r2 = 0.23; P < 0.001), and a weak relationship between delta Ccr and protein intake (r2 = 0.03; P = 0.02), but no relationship between delta Ccr and MAP (r2 = 0.01; P = 0.23). Stepwise multiple regression analysis identified the type of nephropathy, gender, and proteinuria as independent predictive factors of progression; however, these factors together accounted for only 36% of the variation in delta Ccr, suggesting the contribution of other yet unidentified factors.Primary kidney disease and urinary protein excretion (reflecting the severity of renal disease in individual cases) appear as the major determinants of the rate of progression, with faster progression in males in all types of nephropathy, whereas potentially alterable factors such as blood pressure and protein intake had only a modest influence in the range of values observed in our patients.

10.1093/ndt/10.8.1353

Cite

Citations (69)

Neutrophils: Molecules, Functions and Pathophysiological Aspects

Laboratory Investigation (2000)

Véronique Witko‐Sarsat Philippe Rieu Béatrice Descamps‐Latscha Philippe Lesavre Lise Halbwachs‐Mecarelli

Proinflammatory cytokine

Pathophysiology

Neutrophil Extracellular Traps

10.1038/labinvest.3780067

Cite

Citations (1,109)