Michael Vishnevetsky

Mount Sinai Beth Israel

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Alexander Vainstein

Hebrew University of Jerusalem

Marianna Ovadis

Hebrew University of Jerusalem

Yael Libal‐Weksler

Hebrew University of Jerusalem

H. Itzhaki

Mapi Pharma (Israel)

Janvi Paralkar

Mount Sinai Beth Israel

I. Smirra

Hebrew University of Jerusalem

Maggie Levy

Hebrew University of Jerusalem

A. H. Halevy

Hebrew University of Jerusalem

Aloke Chakravarti

Icahn School of Medicine at Mount Sinai

Kristine Favila

Mount Sinai Beth Israel

Cooperative Institutions

Hebrew University of Jerusalem

Mount Sinai Beth Israel

Agricultural Research Organization

Icahn School of Medicine at Mount Sinai

University of Michigan–Ann Arbor

Weizmann Institute of Science

University of California, Davis

Michigan State University

Hertfordshire Community NHS Trust

Pfizer (United States)

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FLOWER-SPECIFIC CAROTENOID ACCUMULATION IN CHROMOPLASTS: MOLECULAR CONTROL OF CAROTENOID-ASSOCIATED PROTEINS

Acta Horticulturae (1995)

Yael Libal‐Weksler Michael Vishnevetsky Marianna Ovadis H. Itzhaki Alexander Vainstein

Chromoplast

10.17660/actahortic.1995.420.7

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Citations (2)

Molecular mechanisms underlying carotenogenesis in the chromoplast: multilevel regulation of carotenoid-associated genes

The Plant Journal (1999)

Michael Vishnevetsky Marianna Ovadis Amir Zuker Alexander Vainstein

In nature, carotenoid function and mode of action are highly determined by the neighboring protein and lipid molecules. Therefore an understanding of the proteins' involvement in carotenoid sequestration would be of great help in elucidating carotenoid function in vivo. Based on a study of the expression of chromoplast-specific carotenoid-associated genes from cucumber corolla (CHRC and CHRD), a working model is presented wherein two major regulatory factors control carotenoid sequestration within the chromoplasts: (i) floral tissue-specific transcriptional regulators of chromoplasto- genesis; and (ii) post-transcriptional regulators related to the amount/type of sequestered carotenoids. This model is supported by the major role transcriptional regulation was found to play in the temporal and spatial expression of the CHRC gene, and by the fact that phytohormones such as gibberellic acid (GA3), abscisic acid and ethylene also acted as transcriptional regulators of CHRC expression. The primary response to GA3 was localized within the CHRC promoter to a 290 bp fragment. Furthermore, we demonstrated strong down-regulation of CHRC expression at post-transcriptional and translational/post-translational levels resulting from inhibition of carotenoid biosynthesis, thus revealing a close link between carotenoid biosynthetic and sequestration machineries.

Chromoplast

Gibberellic acid

10.1046/j.1365-313x.1999.00615.x

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Citations (50)

Molecular cloning of a carotenoid‐associated protein from Cucumis sativus corollas: homologous genes involved in carotenoid sequestration in chromoplasts

The Plant Journal (1996)

Michael Vishnevetsky Marianna Ovadis H. Itzhaki Maggie Levy Yael Libal‐Weksler

Summary Chromoplasts are carotenoid‐accumulating plastids found in the corollas and fruits of many higher plants. In most cases, the pigment in these plastids is accumulated with the aid of carotenoid‐associated proteins located within unique structures. This paper reports the isolation and characterization of the cDNA ( CHRC ) from Cucumis sativus corollas which encodes the chromoplast‐specific carotenoid‐associated protein CHRC. The transit peptide cleavage site was determined and, using a chloroplast uptake system, it is shown that CHRC can be post‐translationally targeted to these plastids where it is peripherally associated with thylakoids. Analysis of CHRC transcript level in Cucumis sativus revealed its temporal and tissue‐specific regulation: the transcript was detected only in corollas, where its level increased in parallel to flower development, peaking just before anthesis. CHRC shares significant homology (59%) with the gene coding for fibrillin — a protein in Capsicum annuum red fruits whose function is essentially identical to that of CHRC. A CHRC fragment including the potential active site of the protein was used as a probe in Northern blot analyses of floral and fruit tissues from various plants containing chromoplasts of different types: CHRC homologs of similar sizes were revealed in all cases. The existence of a group of homologous genes coding for chromoplast‐specific proteins which aid in the sequestration of carotenoids within specific structures is proposed.

Chromoplast

Cucumis

10.1046/j.1365-313x.1996.10061111.x

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Citations (75)

Molecular Control of Flower Development

Springer eBooks (2002)

Michael Vishnevetsky Elliot M. Meyerowitz

Antirrhinum majus

Antirrhinum

Petunia

10.1007/978-94-017-0956-9_12

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Citations (5)

Carotenoid sequestration in plants: the role of carotenoid-associated proteins

Trends in Plant Science (1999)

Michael Vishnevetsky

Chromoplast

10.1016/s1360-1385(99)01414-4

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Citations (231)

Poppers Not Only Make You High: A Rare Case of Amyl Nitrate-Induced Hypoxia

CHEST Journal (2016)

Coral Olazagasti Janvi Paralkar Michael Vishnevetsky Aloke Chakravarti Roxana Sulica

Amyl nitrite

Hypoxia

Methemoglobinemia

Pulse Oximetry

10.1016/j.chest.2016.08.1216

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Citations (1)

Isolation and Regulation of Accumulation of a Minor Chromoplast-Specific Protein from Cucumber Corollas

PLANT PHYSIOLOGY (1997)

Yael Libal‐Weksler Michael Vishnevetsky Marianna Ovadis Alexander Vainstein

Abstract The differentiation of chloroplasts to chromoplasts in cucumber (Cucumis sativus L.) corollas parallels flower development. Chromoplast biogenesis involves chlorophyll degradation, carotenoid accumulation, and the appearance of a new set of proteins. To study factors involved in chromoplast biogenesis in floral tissues, a minor (in abundance) protein of about 14 kD, CHRD (chromoplast protein D), was isolated from cucumber corolla chromoplasts. Immunological characterization revealed that the protein is chromoplast-specific and that its steady-state level in corollas increases in parallel to flower development. The protein was not detected in cucumber leaves or fruits. Immunological analysis of corollas and fruits from a variety of other plants also did not reveal cross-reactivity with the CHRD protein antisera. Using an in vitro bud culture system, we analyzed the effect of phytohormones on CHRD expression. Gibberellic acid rapidly enhanced, whereas paclobutrazol down-regulated, the steady-state level of CHRD. Ethylene also down-regulated the protein's steady-state level. It is suggested that hormonal control of chromoplastogenesis is tightly regulated at the tissue/organ level and that mainly developmental signals control carotenoid accumulation in nonphotosynthetic tissues.

Chromoplast

Cucumis

Gibberellic acid

10.1104/pp.113.1.59

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Citations (13)

Case Study– Youth Gangs and Terrorism in Chechnya: Recruitment, Activities and Networks

Michael Vishnevetsky

This chapter contains sections titled: Introduction Young Soldiers, Terrorists and Violent Youth Gangs Terrorism and the War on Terror in Chechnya Method Youth Groups and Terrorism in Chechnya Conclusion References

10.1002/9780470744499.ch9

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Citations (0)

CHRC, Encoding a Chromoplast-specific Carotenoid-associated Protein, Is an Early Gibberellic Acid-responsive Gene

Journal of Biological Chemistry (1997)

Michael Vishnevetsky Marianna Ovadis H. Itzhaki Alexander Vainstein

Gibberellic acid

Chromoplast

10.1074/jbc.272.40.24747

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Citations (19)

Chromoplast Biogenesis in Cucumis sativus Corollas (Rapid Effect of Gibberellin A3 on the Accumulation of a Chromoplast-Specific Carotenoid-Associated Protein)

PLANT PHYSIOLOGY (1994)

Alexander Vainstein A. H. Halevy I. Smirra Michael Vishnevetsky

The development of cucumber (Cucumis sativus L.) corollas is accompanied by the accumulation of chromoplasts. In mature corollas, chromoplasts, but no chloroplasts, were detected by electron microscopy. Chlorophyll was also undetectable in corollas at anthesis. The contents of carotenoids and a carotenoid-associated, chromoplast-specific, 35-kD protein in corollas increased in parallel with flower development, peaking concomitantly at anthesis. The involvement of phytohormones and light in the regulation of their expression was studied. When gibberellin A3 (GA3) was added to an in vitro bud culture system, accumulation of both carotenoids and the 35-kD protein was markedly enhanced. The specific up-regulation of the 35-kD protein was very rapid: after only 2 h of culture, increased levels were detected in GA3-treated versus untreated corollas. During this period, corolla fresh weight and total protein and carotenoid contents remained unchanged. Inclusion of abscisic acid in the culture medium counteracted the effect of GA3. Accumulation of the 35-kD protein was also enhanced when flower buds on plants were sprayed with GA3 or etiolated.

Chromoplast

Cucumis

Gibberellic acid

Anthesis

Etiolation

Plant Physiology

10.1104/pp.104.2.321

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Citations (25)