Zhu Shen

Southern Medical University

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Ling Chen

Daping Hospital

Hao Fei

Pennsylvania State University

Ling Chen

Tongji Hospital

Gang Wang

Air Force Medical University

Zhifang Zhai

Southwest Hospital

Zhen Xie

University of Electronic Science and Technology of China

Chaonan Sun

Liaoning Cancer Hospital & Institute

Bi Qin

Sichuan Academy of Traditional Chinese Medicine

Jinjin Wu

Chinese Academy of Medical Sciences & Peking Union Medical College

Lü Peng

Beijing Normal University

Cooperative Institutions

Medical University of South Carolina

230

UConn Health

223

American Academy of Dermatology

221

Army Medical University

Air Force Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

Southern Medical University

Southwest Hospital

Sun Yat-sen University

Xijing Hospital

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The effect of antisense tyrosinase-related protein 1 on melanocytes and malignant melanoma cells

British Journal of Dermatology (2004)

C-Y. Li T-W. Gao Gang Wang Z-Y. Han Zhu Shen

Background Tyrosinase-related proteins (TRPs) include tyrosinase, TRP-1 and TRP-2. The functions of tyrosinase and TRP-2 have been determined, but the biological role of TRP-1 is still controversial and is not well known in humans. Objectives To study further the biological role of the human TRP-1 gene in melanocytes and melanoma cells. Methods TRP-1 cDNA was subcloned into eukaryotic expression vector pcDNA3.1 in the reverse direction, and antisense recombinant vector was transfected into melanocytes and a melanoma cell line using Lipofectamine 2000. Positive cells were selected by geneticin. TRP-1 mRNA level was measured by reverse transcription–polymerase chain reaction (RT–PCR), and TRP-1 protein level by Western blot. Cell cycles were determined by flow cytometry, and the activity of tyrosinase was evaluated by L-DOPA reaction. Light microscopy, electron microscopy and flow cytometry were used to observe cell morphology and apoptosis. For in vivo assays, the antitumour activity of antisense TRP-1 against the malignant melanoma (MM) cell line, Libr, was evaluated in an animal-tumour model of subcutaneous tumours. Results Positive transfected cells steadily expressed TRP-1 antisense RNA. RT–PCR and Western blot showed a low level of TRP-1 mRNA and TRP-1 protein, respectively. Cell cycles were blocked in the G1 stage, and the activity of tyrosinase decreased significantly (P < 0·01). Light and electron microscopy showed abnormal cell morphology, and apoptosis was detected. The neoplasia activity of antisense TRP-1-transfected MM cells was significantly lower than that of MM cells (P < 0·01). Conclusions TRP-1 plays an important role in the proliferation, morphology and tyrosinase activity of melanocytes and melanoma cells.

10.1111/j.1365-2133.2004.05929.x

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Altered keratin 17 peptide ligands inhibit in vitro proliferation of keratinocytes and T cells isolated from patients with psoriasis

Journal of the American Academy of Dermatology (2006)

Zhu Shen Ling Chen Yu-F. Liu Tian-W. Gao Gang Wang

10.1016/j.jaad.2006.02.033

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Citations (44)

Editorial Board

Journal of the American Academy of Dermatology (2022)

Dirk M. Elston Jane M. Grant‐Kels James C. Smith Richard Dobson Jeffrey D. Bernhard

10.1016/s0190-9622(22)02697-4

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097 Tissue-resident memory T cells in psoriasis recurrence

Journal of Investigative Dermatology (2018)

Zhu Shen

Epidermis (zoology)

Interleukin-7 receptor

10.1016/j.jid.2018.03.101

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Correction: Clinical Characteristics and Therapeutic Aspects of Blaschko Linear Psoriasis

Dermatology and Therapy (2024)

Ling Chen Yu‐Fan Cheng Lü Peng Xuesong Jia Guangren Liu

10.1007/s13555-024-01194-0

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Citations (1)

Clinical Characteristics and Therapeutic Aspects of Blaschko Linear Psoriasis

Dermatology and Therapy (2024)

Ling Chen Yu‐Fan Cheng Lü Peng Xuesong Jia Guangren Liu

Blaschko linear psoriasis (BLP) is characterized by the linear distribution of psoriatic skin lesions along the Blaschko lines. BLP can be divided into type I and type II, mainly on the basis of clinical manifestations. BLP can easily cause psychological burdens in patients and clinical confusion for physicians. Here, we summarize clinical cases to provide a better understanding of BLP.

Skin type

Histopathology

10.1007/s13555-024-01140-0

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Citations (4)

Clinical guideline for the diagnosis and treatment of cutaneous warts (2022)

Journal of Evidence-Based Medicine (2022)

Peiyao Zhu Ruiqun Qi Yang� Yang Wei Huo Yuqing Zhang

Cutaneous warts caused by human papillomavirus are benign proliferative lesions that occur at any ages in human lives. Updated, comprehensive and systematic evidence-based guidelines to guide clinical practice are urgently needed.We collaborated with multidisciplinary experts to formulate this guideline based on evidences of already published literature, focusing on 13 clinical questions elected by a panel of experts. We adopted Grading of Recommendations Assessment, Development and Evaluation (GRADE) system to form classification of recommendations as well as the improved Delphi method to retain respective recommendations with a consensus degree of over 80%.Our guideline covered aspects of the diagnosis and treatment of cutaneous warts such as diagnostic gold standard, transmission routes, laboratory tests, treatment principle, clinical cure criterion, definitions, and treatments of common warts, flat warts, plantar warts, condyloma acuminatum, and epidermodysplasia verruciformis. Recommendations about special population such as children and pregnant women are also listed. In total, 49 recommendations have been obtained.It is a comprehensive and systematic evidence-based guideline and we hope this guideline could systematically and effectively guide the clinical practice of cutaneous warts and improve the overall levels of medical services.

Guideline

10.1111/jebm.12494

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Citations (42)

Mechanisms of gastrointestinal microflora on drug metabolism in clinical practice

Saudi Pharmaceutical Journal (2019)

Chaonan Sun Ling Chen Zhu Shen

Considered as an essential "metabolic organ", intestinal microbiota plays a key role in human health and the predisposition to diseases. It is an aggregate genome of trillions of microorganisms residing in the human gastrointestinal tract. Since the 20th century, researches have showed that intestinal microbiome possesses a variety of metabolic activities that are able to modulate the fate of more than 30 approved drugs and immune checkpoint inhibitors. These drugs are transformed to bioactive, inactive, or toxic metabolites by microbial direct action or host-microbial co-metabolism. These metabolites are responsible for therapeutic effects exerted by these drugs or side effects induced by these drugs, even for death. In view of the significant effect on the drugs metabolism by the gut microbiota, it is pivotal for personalized medicine to explore additional drugs affected by gut microbiota and their involved strains for further making mechanism clear through suitable animal models. This review mainly focus on specific mechanisms involved, with reference to the current literature about drugs metabolism by related bacteria or its enzymes available.

Microbial Metabolism

10.1016/j.jsps.2019.09.011

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Citations (68)

Skin microbial dysbiosis is a characteristic of systemic drug-related intertriginous and flexural exanthema-like lesions induced by EGFR inhibitor

Heliyon (2023)

Wenqi Liu Lu Peng Ling Chen Jianji Wan Shuang Lou

ObjectivesTo investigate the characteristics of the skin microbiome in severe afatinib-induced skin toxicity.MethodsBody site-matched skin surface samples were collected from the lesions on seven flexural sites of one lung cancer (Patient 1) with serious systemic drug-related intertriginous and flexural exanthema (SDRIFE)-like toxicity induced by EGFR-TKI and three healthy age/sex matched controls for whole metagenomics sequencing analysis. Lung cancer Patient 1 and Patient 2 were prescribed minocycline and followed up.ResultsIn SDRIFE-like toxicities induced by afatinib, lesion microbiota richness (ACE and Chao1 index: p < 0.001) and diversity (Shannon's and Simpson's diversity indices: p < 0.01) were reduced. Similarly, the beta diversity analysis (R = 1, p = 0.002 for ANOSIM) showed that the apparent difference in the microbiota composition was statistically significant. The microbial taxa composition in the patient showed an increased abundance of pathogenic bacteria and a decreased abundance of commensal bacteria. LEfSe analysis identified strong bacterial pathogenicity in the patient, while healthy controls exhibited enrichment in several pathways that are beneficial for skin commensal bacteria and skin physiology, including key amino acid metabolism, energy/lipid/glycan biosynthesis/metabolism, and cofactors/vitamins biosynthesis. Ultimately, the patients experienced significant improvement with minocycline.ConclusionMicrobial dysbiosis is a characteristic of severe SDRIFE-like toxicity induced by afatinib.

Intertriginous

Dysbiosis

10.1016/j.heliyon.2023.e21690

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Tissue‐resident memory T cells in immunotherapy and immune‐related adverse events by immune checkpoint inhibitor

International Journal of Cancer (2024)

Hao Xiong Zhu Shen

Tissue-resident memory T cells (TRM) are a specialized subset of T cells that reside in tissues and provide long-term protective immunity against pathogens that enter the body through that specific tissue. TRM cells have specific phenotype and reside preferentially in barrier tissues. Recent studies have revealed that TRM cells are the main target of immune checkpoint inhibitor immunotherapy since their role in cancer immunosurveillance. Furthermore, TRM cells also play a crucial part in pathogenesis of immune-related adverse events (irAEs). Here, we provide a concise review of biological characteristics of TRM cells, and the major advances and recent findings regarding their involvement in immune checkpoint inhibitor immunotherapy and the corresponding irAEs.

Immunosurveillance

Cancer Immunotherapy

Immune checkpoint

10.1002/ijc.34940

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