V. A. Mikhailova

Research Institute of Obstetrics and Gynecology named after D.O. Ott

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ДИ

Д. И. Соколов

Research Institute of Obstetrics and Gynecology named after D.O. Ott

SА

S. А. Selkov

Research Institute of Obstetrics and Gynecology named after D.O. Ott

K. L. Markova

Research Institute of Obstetrics and Gynecology named after D.O. Ott

D. O. Bazhenov

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Igor Yu. Kogan

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Polina Grebenkina

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Yu. P. Milyutina

Research Institute of Obstetrics and Gynecology named after D.O. Ott

A. R. Sheveleva

Research Institute of Obstetrics and Gynecology named after D.O. Ott

АV

А. V. Korenevsky

Research Institute of Obstetrics and Gynecology named after D.O. Ott

K. L. Belyakova

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Cooperative Institutions

Research Institute of Obstetrics and Gynecology named after D.O. Ott

Russian Academy of Sciences

St Petersburg University

Ministry of Health of the Russian Federation

First Pavlov State Medical University of St. Petersburg

Academy of Medical Sciences

Saint Petersburg Pasteur Institute

Institute of Cytology

Federal Almazov North-West Medical Research Centre

Institute of Experimental Medicine

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[Onchocerciasis confirmed by detection of the parasites in the skin and eyes].

PubMed (1980)

V. A. Mikhailova F M Olinskaia

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Immunological test of combined pertussis-diphtheria-tetanus vaccine with diminished doses of individual components.

Zhurnal mikrobiologii epidemiologii i immunobiologii (1973)

A Polikar K Solomonova V. A. Mikhailova Pepa Atanasova Khr. Khadzhikoleva

Pertussis vaccine

Diphtheria Toxin

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NATURAL KILLER CELL EFFECTS UPON ANGIOGENESIS UNDER CONDITIONS OF CONTACT-DEPENDENT AND DISTANT CO-CULTURING WITH ENDOTHELIAL AND TROPHOBLAST CELLS

Medical Immunology (Russia) (2019)

K. L. Markova О. И. Степанова A. R. Sheveleva Nikolay Anatolievich Kostin V. A. Mikhailova

Regulation of angiogenesis in the utero-placental bed determines adequate trophoblast invasion, placenta formation and development, as well as successful course of pregnancy. Natural killer (NK) cells, macrophages and trophoblast have the most significant effect on angiogenesis. To date, the functions of cells participating in placenta formation have been described in detail, both individually ( in vitr о ) and in tissues ( in situ ). However, no models have yet been created that reflect the interactions of NK cells, trophoblast and endothelium during angiogenesis. It remains unclear, how each cell population contributes to placental angiogenesis regulation, and to the cross-regulation of participating cell functions. Therefore, the aim of this research was to study contact and distant effects of NK cells upon formation of tube-like structures through co-culture of endothelial and trophoblast cells influenced by various cytokines (bFGF, VEGF, PlGF, TGF-β, IL-8, IFNγ and IL-1β). Introduction of NK cells to the co-culture of endothelial and trophoblast cells under conditions of both contact and distance-dependent culturing did not change the length of tube-like structures formed by endothelial cells. During contact-dependent culturing of NK cells with co-culture of endothelial and trophoblast cells in presence of IL-1β, the length of tubule-like structures remained unchanged, compared with the length of tube-like structures formed under the same culturing conditions, but without the cytokine added. During distant culturing of NK cells with co-culture of endothelial and trophoblast cells in the presence of IL-1β, the length of tube-like structures increased as compared with those formed under the same culturing conditions but without the cytokine. During contact-dependent (but not distant) culturing of NK cells with the co-culture of endothelial and trophoblast cells in the presence of VEGF, the length of tube-like structures was greater than those formed under the same culturing conditions but without the cytokine. When used in a three-component cell system, the pro-inflammatory cytokine IFNγhad no effect upon angiogenesis. During distant (but not contact-dependent) culturing of NK cells with co-culture of endothelial and trophoblast cells in the presence of TGF-β, the length of tube-like structures was less than the length of tube-like structures formed under the same culturing conditions but without the cytokine. Under conditions of distant culturing, TGF-βtriggered a signal in NK cells that inhibited angiogenesis. Decreased length of tube-like structures under conditions of a three-component cell co-culture in the presence of the following pro-angiogenic factors was revealed: IL-8, PlGF (during contact-dependent culturing only) and bFGF (during both contact-dependent and distant culturing). Thus, the effects of cytokines upon angiogenesis in a three-component co-culture (NK cells, trophoblast and endothelium) differed from those revealed previously in single-component (endothelium only) and two-component (co-culture of endothelium and trophoblast) cell models. The results of these experiments indicated that regulation of placental cell interactions involved both cellular contacts and effects produced by cytokines.

Trophoblast

10.15789/1563-0625-2019-3-427-440

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Characteristics of Natural Killer Cell Interaction with Trophoblast Cells During Pregnancy

Current Molecular Medicine (2019)

D. O. Bazhenov Evgeniya V. Khokhlova Larisa Viazmina K. N. Furaeva V. A. Mikhailova

Maternal natural killer cells (NK cells) are a prevailing leukocyte population in the uteroplacental bed. Current descriptions of the effect of cytokines from the placental microenvironment on the expression of receptors by trophoblast and NK cells are inadequate and contradictory. There is insufficient information about the ability of NK cells to migrate through trophoblast cells.To assess the impact of conditioned media obtained during culturing of placentas from the first and the third trimesters of healthy pregnancies on the phenotype of trophoblast and NK cells and impact on adhesion and transmigration of NK cells through trophoblast cell layer.We established that conditioned media obtained from both first and third trimester placentas increased the intensity of CD106, CD49e, CD49a, CD31, CD51/61, and integrin β6 expression by trophoblast cells. Conditioned media obtained from first trimester placentas increased the intensity of CD11a, CD29, CD49d, CD58, CD29 expression by NK cells. The presence of conditioned media from third trimester placentas resulted in more intense CD29, CD49d, CD11a, CD29, CD49d, and CD58 expression by NK cells. Migration of NK cells through trophoblast cells in the presence of conditioned media from first trimester placentas was increased compared with the migration level in the presence of conditioned media from third trimester placentas. This may be associated with increased expression of CD18 by NK cells.First trimester placental secretory products increase adhesion receptor expression by both trophoblast and NK cells. Under these conditions, trophoblast is capable of ensuring NK cell adhesion and transmigration.

Trophoblast

CD11a

10.2174/1566524019666190808103227

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Врожденные пороки развития. Синдром Смита — Лемли — Опица

The Neurological Journal (2013)

А.М. Милованова В. Чернышева И. Чубарова В. Калинина V. A. Mikhailova

The article presents the literature review of Smith-Lemley-Opitz syndrome in children. Particular attention is paid to symptoms and antemortem and postmortem diagnosis of the disease. The own case report of this hereditary disease is presented in the article.

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Prospects of neuromodulation for chronic pain

Brain Disorders (2021)

E D Isagulyan V. A. Mikhailova K S Aslakhanova Konstantin V. Slavin

For over half a century neuromodulation has been successfully used for treatment of chronic pain syndromes. There is a growing number of new modes or targets of neurostimulation, a widening of indications and continuous improvement in existing approaches and steady reduction in complications. Nowadays the field of neuromodulation is progressively evolving showing significant advancement in therapeutic efficacy. This review focuses on the current state and the future development of the art of neuromodulation in treatment of chronic pain. The field of neuromodulation continues to evolve with improvement in clinical outcomes for chronic pain patients. The new advances in neuromodulation along with new discoveries in neurosciences are expected to translate into better results in terms of both efficacy and safety.

Neuromodulation

Neurostimulation

10.1016/j.dscb.2021.100027

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Microvesicles produced by monocytes affect the phenotype and functions of endothelial cells

AIMS Allergy and Immunology (2021)

Д. И. Соколов А. Р. Козырева K. L. Markova V. A. Mikhailova А. В. Кореневский

Monocytes\macrophages regulate angiogenesis via cytokine production and contact interactions with endothelial cells (ECs). The biological effects of macrophage-derived microvesicles (MVs) are studied using cell lines, such as monocytic leukemia THP-1 cell line. The effect of MVs produced by THP-1 cells on EC phenotype and functions remain understudied. In this research, we studied the effect of MVs produced by THP-1 cells on the phenotype, proliferation, migration, and vascular formation of EA.Hy926 ECs. MVs produced by THP-1 cells express CD54, CD18, CD11a, CD11b, CD29, CD120a, CD120b, VEGFR1, VEGFR2, CD105, CD119, TGFR2 on the surface and contain ERK1/2, pERK1/2 Akt, FGF10, endothelin-2. The transfer of an intracellular protein labeled with a fluorescent dye from MVs produced by THP-1 cells to EA.Hy926 ECs was established. It was found that MVs derived from THP-1 cells inhibit EC proliferation. In high concentrations, MVs reduce EC migration, increase the length but decrease the number of vessels formed by ECs, promoting the development of non-branching angiogenesis. On the contrary, in low concentrations, MVs increase EC migration, reduce the length, and increase the number of vessels formed by ECs, promoting the development of branching angiogenesis. Thus, the fundamental possibility of the influence of MVs produced by THP-1 cells on the processes of angiogenesis has been established. Proteins found in the MVs composition may be responsible for the observed effects of MVs on ECs.

THP1 cell line

10.3934/allergy.2021011

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Microvesicles derived from leukocytes in the peripheral blood of patients with external genital endometriosis

Medical Immunology (Russia) (2022)

Maria I. Yarmolinskaya E.I. Durneva K. L. Markova V. A. Mikhailova S. А. Selkov

Endometriosis is a chronic gynecological disease, which poses a serious problem in terms of diagnosis and treatment. Despite decades of research, there are no specific signs and symptoms and no blood tests to clinically confirm the diagnosis, which makes timely diagnosis and treatment difficult. Therefore, the search for new markers for early non-invasive diagnosis of the disease remains relevant. Various subcellular structures involved in intercellular communication, in particular, microvesicles, can be considered promising biological markers for external genital endometriosis. The aim of this work was to assess the composition of microvesicles derived from leukocytes in the peripheral blood of patients with stage I-II of external genital endometriosis and the possibility of their use as markers of non-invasive diagnosis of peritoneal forms of endometriosis. The study involved 97 women aged 26-40 with stage I-II of external genital endometriosis, whose diagnosis was established intraoperatively and confirmed histologically. Pain syndrome was noted in all patients of the main group, with infertility also detected in 73.2% of the patients. The control group consisted of 20 patients, whose average age was 25.5±1.1 years, who were examined in connection with male infertility factor before the in vitro fertilization, and in whom, on the basis of intraoperative examination, presented no gynecological diseases, and no pain syndrome. Before the surgical intervention, peripheral blood was taken from all patients to determine the content of microvesicles derived from leukocytes. To isolate microvesicles, we used the previously described by M.P. Gelderman and J. Simak method. It was found that patients with stage I-II of external genital endometriosis experience an increase in the number of CD14 + , CD16 + and CD54 + CD14 + microvesicles in the peripheral blood by 1.1, 1.38 and 1.55 times, respectively, as well as a decrease in the number of CD45 + CD4 + , CD3 + CD4 + , CD3 + CD8 + microvesicles by 1.2, 4 and 1.5 times, respectively, compared with patients from the control group. Therefore, in patients with stage I-II of external genital endometriosis, an increase in the relative number of CD54 + CD14 + microvesicles in the peripheral blood above 5.22% can serve as a marker for early non-invasive diagnosis of the disease with sensitivity of 80.5% and specificity of 71%.

Sex organ

10.15789/1563-0625-mdf-2447

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Microvesicles produced by natural killer cells of the NK-92 cell line affect the phenotype and functions of endothelial cells of the EA.Hy926 cell line

Medical Immunology (Russia) (2020)

K. L. Markova V. A. Mikhailova А. V. Korenevsky Yu. P. Milyutina Veronika Rodygina

Microvesicles (MVs) are small (100-1000 nm) subcellular structures produced by both motionless and activated cells that can transfer molecules to target cells, and regulate physiological and pathological processes. MVs of leukocyte origin, in particular those produced by natural killer cells (NK cells), remain the least studied population of MVs. NK cells can change the functional activity of endothelial cells (ECs) and are involved in regulating angiogenesis. The ability of NK cell-derived MVs to influence the functionality of ECs is understudied currently. We aimed to study the effect of MVs produced by NK cells of the NK-92 cell line on the phenotype, caspase activity, proliferation and migration of ECs of the EA.Hy926 cell line. We cultured ECs in the presence of MVs derived from the NK-92 cell line, and then used flow cytometry to assess changes in EC phenotype, intracellular protein transfer from MVs to ECs, and the relative death of ECs. We used western blot analysis to evaluate the expression of granzyme B in NK cells and in the MVs that they produced, as well as the expression of granzyme B, caspases, extracellular-regulated kinase (ERK) and protein kinase B (AKT) in ECs. We also assessed the proliferation and migration of ECs in the presence of MVs derived from cells of the NK-92 cell line. The results revealed significant differences in the proteomic profiles of cells of the NK-92 cell line and their MV product. Contact between ECs and MVs derived from cells of the NK-92 cell line is accompanied by the following events: a) expression of granzyme B in ECs, b) activation of caspase-9 and caspase-3, with partial EC death, c) appearance of the panleukocyte marker CD45 on ECs, d) decrease in CD105 expression, and increase in CD34 and CD54 expression, and e) inhibition of EC migration. Transfer of ERK (but not AKT) from MVs derived from cells of the NK-92 cell line to ECs, at a concentration 10 times lower than that which causes EC death, leads to an increase in EC proliferation.

Granzyme

Lymphokine-activated killer cell

10.15789/1563-0625-mpb-1877

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Spinal Cord Stimulation for Spinal Cord Injury

IntechOpen eBooks (2022)

E D Isagulyan V. A. Mikhailova Nikita Ilyinski

Spinal cord injury is a medically complex and life-disrupting condition, associated with very high mortality rates (early death rates after admission range from 4 to 20%). In addition, it’s complicated subsequent severe disability due to the development of early or late complications. Today, in high-income countries, SCI can be viewed less as the end of a worthwhile or productive life and more as a personal and social challenge that can be successfully overcome. SCI can be divided into two types of injury on the basis of severity: complete and incomplete injury. Damage to the spinal cord may be traumatic (falls, road traffic injuries, occupational and sports injuries, violence) or non-traumatic (infectious disease, tumor, musculoskeletal disease, congenital problems such as spina bifid).

10.5772/intechopen.105005

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