Shinji Sugiura

National Institute of Advanced Industrial Science and Technology

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Toshiyuki Kanamori

National Institute of Advanced Industrial Science and Technology

Kimio Sumaru

National Institute of Advanced Industrial Science and Technology

Koji Hattori

Denso (Japan)

Mitsutoshi Nakajima

University of Tsukuba

Toshiyuki Takagi

National Institute of Advanced Industrial Science and Technology

Taku Satoh

University of Tsukuba

Fumiki Yanagawa

National Institute of Advanced Industrial Science and Technology

Sosaku Ichikawa

University of Tsukuba

Minoru Seki

Chiba University

Masato Tamura

Topcon (Japan)

Cooperative Institutions

National Institute of Advanced Industrial Science and Technology

The University of Tokyo

University of Tsukuba

Kyoto University

Nagaoka University of Technology

Tokyo University of Science

Nagoya University

Osaka University

Tokyo Institute of Technology

Japan Science and Technology Agency

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Comparison of substance supply in static and perfusion cultures based on mass transport phenomena

Biochemical Engineering Journal (2011)

Shinji Sugiura Toshiyuki Kanamori

Mass transport

10.1016/j.bej.2011.08.010

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Citations (2)

A multi-throughput multi-organ-on-a-chip system on a plate formatted pneumatic pressure-driven medium circulation platform

Lab on a Chip (2017)

Takuya Satoh Shinji Sugiura Kazumi Shin Reiko Onuki-Nagasaki Susumu Ishida

This paper reports a multi-throughput multi-organ-on-a-chip system formed on a pneumatic pressure-driven medium circulation platform with a microplate-sized format as a novel type of microphysiological system. The pneumatic pressure-driven platform enabled parallelized multi-organ experiments (i.e. simultaneous operation of multiple multi-organ culture units) and pipette-friendly liquid handling for various conventional cell culture experiments, including cell seeding, medium change, live/dead staining, cell growth analysis, gene expression analysis of collected cells, and liquid chromatography-mass spectrometry analysis of chemical compounds in the culture medium. An eight-throughput two-organ system and a four-throughput four-organ system were constructed on a common platform, with different microfluidic plates. The two-organ system, composed of liver and cancer models, was used to demonstrate the effect of an anticancer prodrug, capecitabine (CAP), whose metabolite 5-fluorouracil (5-FU) after metabolism by HepaRG hepatic cells inhibited the proliferation of HCT-116 cancer cells. The four-organ system, composed of intestine, liver, cancer, and connective tissue models, was used to demonstrate evaluation of the effects of 5-FU and two prodrugs of 5-FU (CAP and tegafur) on multiple organ models, including cancer and connective tissue.

Circulation (fluid dynamics)

10.1039/c7lc00952f

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Citations (145)

ChemInform Abstract: PHOTOINDUCED TRANSFORMATIONS. PART 39. FRAGMENTATION OF HYPOIODITES AND RELATED REACTIONS INVOLVING OXYL RADICALS OF STEROIDAL ALCOHOLS WITH A VICINAL ACETYLAMINO OR ACETOXY FUNCTION

Chemischer Informationsdienst (1979)

H. SUGINOME Hiroo Umeda Shinji Sugiura Miyasaka Tadashi

Abstract Die Hypoiodit‐Reaktion der Steroide (I) bzw. (III) unter Bedingungen der Methoden A)‐C) führt über Oxylradikale zu den Aldehyden (II) bzw. (IV), wobei die Umsetzung von (III) nach C) zusätzlich den cyclischen Ether (V) liefert; das Steroid (VI) wird mit Pb(O‐Ac)4 zu dem Aldehyd (VII) oxidiert.

Vicinal

10.1002/chin.197906098

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Cypridina bioluminescence v. structure of emitting species in the luminescence of cypridina luciferin and its related compounds

Tetrahedron Letters (1968)

Toshio Goto Shoji Inoue Shinji Sugiura K Nishikawa Minoru Isobe

Luciferin

Photoprotein

10.1016/s0040-4039(00)76313-1

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Citations (49)

MN-P24 Preparation of calcium alginate microbeads from water-in-oil-in-water emulsions using microfluidic device(Section X Micro/Nano Technology for Analysis and Cell Manipulation)

Journal of Bioscience and Bioengineering (2009)

Daisuke Saeki Shinji Sugiura Toshiyuki Kanamori Seigo Sato Sosaku Ichikawa

Water in oil

Calcium alginate

Section (typography)

Microemulsion

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Integrated perfusion culture microchamber array chip with serial dilution microfluidic network for high throughput drug dose response assay

Journal of Bioscience and Bioengineering (2009)

Shinji Sugiura Koji Hattori Toshiyuki Kanamori

Dilution

Microfluidic chip

10.1016/j.jbiosc.2009.08.414

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Microfluidic perfusion culture of human induced pluripotent stem cell in microchamber array chip

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (2013)

Ryosuke Yoshimitsu Koji Hattori Shinji Sugiura Yuki Kondo Taroh Satoh

This paper reports on the microfluidic perfusion culture of human induced pluripotent stem cells (hiPSCs) under fully defined culture condition. By screening various ECMs we determined that fibronectin and laminin are appropriate for microfluidic devices made out of the most popular material, polydimethylsiloxane (PDMS). We found that the growth rate of hiPSCs under pressure-driven perfusion culture conditions was higher than under static culture conditions in the microchamber array. We believe that our system will be a platform technology for future large-scale screening of fully defined conditions for differentiation cultures on integrated microfluidic devices.

Polydimethylsiloxane

Microfluidic chip

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Dynamic three-dimensional micropatterned cell co-cultures within photocurable and chemically degradable hydrogels

Journal of Tissue Engineering and Regenerative Medicine (2013)

Shinji Sugiura Jae Min Fumiki Yanagawa Pınar Zorlutuna Hojae Bae

In this paper we report on the development of dynamically controlled three-dimensional (3D) micropatterned cellular co-cultures within photocurable and chemically degradable hydrogels. Specifically, we generated dynamic co-cultures of micropatterned murine embryonic stem (mES) cells with human hepatocellular carcinoma (HepG2) cells within 3D hydrogels. HepG2 cells were used due to their ability to direct the differentiation of mES cells through secreted paracrine factors. To generate dynamic co-cultures, mES cells were first encapsulated within micropatterned photocurable poly(ethylene glycol) (PEG) hydrogels. These micropatterned cell-laden PEG hydrogels were subsequently surrounded by calcium alginate (Ca-Alg) hydrogels containing HepG2 cells. After 4 days, the co-culture step was halted by exposing the system to sodium citrate solution, which removed the alginate gels and the encapsulated HepG2 cells. The encapsulated mES cells were then maintained in the resulting cultures for 16 days and cardiac differentiation was analysed. We observed that the mES cells that were exposed to HepG2 cells in the co-cultures generated cells with higher expression of cardiac genes and proteins, as well as increased spontaneous beating. Due to its ability to control the 3D microenvironment of cells in a spatially and temporally regulated manner, the method presented in this study is useful for a range of cell-culture applications related to tissue engineering and regenerative medicine. Copyright © 2013 John Wiley & Sons, Ltd.

Micropatterning

Cell encapsulation

Regenerative Medicine

10.1002/term.1843

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Citations (17)

Partially photodegradable hybrid hydrogels with elasticity tunable by light irradiation

Colloids and Surfaces B Biointerfaces (2014)

Fumiki Yanagawa Takeomi Mizutani Shinji Sugiura Toshiyuki Takagi Kimio Sumaru

Elasticity

Microscale chemistry

Tetra