Hosny M. Behnsawy

Assiut University

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Masato Fujisawa

Kobe University

Hideaki Miyake

Kobe University

Katsumi Shigemura

Hyogo University of Health Sciences

Toshiro Shirakawa

Kobe University

Fukashi Yamamichi

Hyogo Prefectural Amagasaki General Medical Center

Diaa A. Hameed

Assiut University

Masuo Yamashita

Shinko Hospital

Ahmed M. Moeen

Assiut University

Fatma Y. Meligy

Assiut University

Masato Kawabata

Rikkyo University

Cooperative Institutions

Assiut University

Kobe University

Assiut University Hospitals

Tokyo Medical and Dental University

Charité - Universitätsmedizin Berlin

Klinik und Poliklinik für Urologie

München Klinik

Kobe University Hospital

Technical University of Munich

Klinikum rechts der Isar

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The Effects of COVID-19 on Spermatogenesis

Journal of Antivirals & Antiretrovirals (2021)

Mostafa Kamel Ahmed Abde Moniem Mohammed Ali Zarzour Ade Kurkar Hosny M. Behnsawy

Objective: To observe changes in sperm parameters after 72 days of infection by COVID-19. Methods: A total of 100 patients had been enrolled in the study by a criteria suggesting good semen analysis. Two sets of semen analysis done, the first after 72 days of first positive swab for COVID-19 to show changes in the cycle of spermatogenesis during infection, the other sample after 72 days from the first to compare it with the first sample. Results: A total number 100 patients first sample show 2% of patient’s oligospermia, 36% of patient’s teratospermia. The second sample shows 4% of patients’ teratospermia. by comparing the two samples there is a significant increase in sperm concentration also a significant increase in motility (A+B), a highly significant increase in the normal forms of sperms. Conclusion: COVID-19 affects spermatogenesis in the form of reversible teratospermia, reversible decrease sperm count but within normal level, reversible decrease in the sperm motility but also within normal level.

Oligospermia

Semen Analysis

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1047 Expression of epithelial-mesenchymal transition markers in localized prostate cancer: Impact on clinicopathological outcomes in patients undergoing radical prostatectomy

European Urology Supplements (2013)

Hideaki Miyake Hosny M. Behnsawy M. Fujisawa

10.1016/s1569-9056(13)61523-7

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146 POSSIBLE ROLE OF SONIC HEDGEHOG SIGNALING AND THE LINK WITH EPITHELIAL-MESENCHYMAL TRANSITION (EMT) IN RENAL CANCER PROGRESSION

The Journal of Urology (2012)

Fatma Y. Meligy Katsumi Shigemura Hosny M. Behnsawy Fukashi Yamamichi Masuo Yamashita

You have accessJournal of UrologyKidney Cancer: Basic Research I1 Apr 2012146 POSSIBLE ROLE OF SONIC HEDGEHOG SIGNALING AND THE LINK WITH EPITHELIAL-MESENCHYMAL TRANSITION (EMT) IN RENAL CANCER PROGRESSION Fatma Meligy, Katsumi Shigemura, Hosny Behnsawy, Fukashi Yamamichi, Masuo Yamashita, Wen-chin Haung, Li Xiangyan, Leland Chung, Masato Fujisawa, Masato Kawabata, and Toshiro Shirakawa Fatma MeligyFatma Meligy Kobe, Japan , Katsumi ShigemuraKatsumi Shigemura Kobe, Japan , Hosny BehnsawyHosny Behnsawy Kobe, Japan , Fukashi YamamichiFukashi Yamamichi Kobe, Japan , Masuo YamashitaMasuo Yamashita Kobe, Japan , Wen-chin HaungWen-chin Haung Kobe, Japan , Li XiangyanLi Xiangyan Los Angeles, CA , Leland ChungLeland Chung Los Angeles, CA , Masato FujisawaMasato Fujisawa Kobe, Japan , Masato KawabataMasato Kawabata Kobe, Japan , and Toshiro ShirakawaToshiro Shirakawa Kobe, Japan View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.196AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Research for new molecular targeting therapy in renal cell carcinoma (RCC) is required. The Objective of this study was to investigate the link of Sonic hedgehog (Shh) signaling with epithelial-mesenchymal transition (EMT) for RCC progression, and determine the prognostic marker for clinical staging or grading. METHODS Alamar Blue cell proliferation assay in mouse RCC cell (RenCa) was investigated under the presence or absence of Shh signaling stimulator, recombinant Shh protein and/or Shh signaling inhibitor, cyclopamine (Cyc). Real time RT-PCR was performed to see expression of Gli-1, EMT markers (E-cadherin, N-cadherin, and Vimentin), and Osteonection (ON), shown as one possible Shh target gene in our previous work of another cancer (prostate cancer) with or without recombinant Shh protein and/or Cyc. Expression levels of Gli-1, Ki-67, Shh, ON, and EMT markers (E-cadherin, N-cadherin and Vimentin) in radical nephrectomy specimens from RCC patients were measured by immunohistochemical (IHC) staining. RESULTS RenCa cells showed enhanced cell proliferation by recombinant Shh protein and suppressed cell proliferation by Cyc in dose dependent manner. Real time RT-PCR results showed Shh enhanced the expression of Gli-1 and ON, and induced EMT (increased the expressions of N-cadherin and Vimentin and suppressed the expressions of E-cadherin) and those inductions were blocked by addition of Cyc. Our IHC staining results showed that there was an inverse significant correlation of expression of N-cadherin and Gli-1 and of expression of Vimentin and ON, respectively (p<0.05). In addition, tumor grade has a significant correlation with expression of EMT markers (N-cadherin and E-cadherin) (p<0.05) and pathological T stage had a significant correlation with ON expression (p<0.05). With regards to tumor infiltration form, there was a significant relationship with the expressions of EMT markers (N-cadherin and E-cadherin) and a significant inverse correlation with the ON expression (p<0.05). These results, taken together, suggest the link of Shh signaling with EMT in RCC progression. CONCLUSIONS Shh signaling and the link with EMT play a pivotal role in RCC progression. Hedgehog signaling inhibitor, cyclopamine may be one possible molecular targeted therapeutic strategy for RCC. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e60-e61 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Fatma Meligy Kobe, Japan More articles by this author Katsumi Shigemura Kobe, Japan More articles by this author Hosny Behnsawy Kobe, Japan More articles by this author Fukashi Yamamichi Kobe, Japan More articles by this author Masuo Yamashita Kobe, Japan More articles by this author Wen-chin Haung Kobe, Japan More articles by this author Li Xiangyan Los Angeles, CA More articles by this author Leland Chung Los Angeles, CA More articles by this author Masato Fujisawa Kobe, Japan More articles by this author Masato Kawabata Kobe, Japan More articles by this author Toshiro Shirakawa Kobe, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

10.1016/j.juro.2012.02.196

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160 Expression of cell cycle-associated proteins in non-muscle invasive bladder cancer: Correlation with intravesical recurrence following transurethral resection

European Urology Supplements (2012)

Hideaki Miyake Hosny M. Behnsawy M. Fujisawa

10.1016/s1569-9056(12)60158-4

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Functional evaluation of a spiral neobladder with an angled chimney; A prospective randomized comparative study

European Journal of Surgical Oncology (2022)

Ahmed M. Moeen Hosny M. Behnsawy Mohammad A. Faragallah Rabea Ahmed Gadelkareem Mahmoud M. Osman

Chimney (locomotive)

10.1016/j.ejso.2022.09.021

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Limited Significance of Activated Akt-Mammalian Target of Rapamycin Signaling Pathway in Prostate Cancer Progression

Urologia Internationalis (2014)

Young Hwii Ko Hideaki Miyake Hosny M. Behnsawy Jun Cheon Masato Fujisawa

Objective: The objective of this study was to investigate the significance of the activated Akt-mammalian target of rapamycin (Akt-mTOR) signaling pathway in the progression of prostate cancer. Materials and Methods: The expression levels of Akt, phosphorylated Akt (p-Akt), mTOR and phosphorylated mTOR (p-mTOR) in 175 prostate specimens, including 61 normal prostate tissues as a control, 24 high-grade prostatic intraepithelial neoplasias (HGPINs) and 90 clinically localized prostate cancers, were evaluated by immunohistochemical staining. p-Akt and p-mTOR ratios, which were defined as the expression level of p-Akt in relation to that of Akt and the expression level of p-mTOR in relation to that of mTOR, respectively, in these specimens were calculated. Results: Expression levels of all four molecules, including Akt, p-Akt, mTOR and p-mTOR, were significantly greater in the HGPIN group compared with the normal control and prostate cancer groups. Furthermore, the p-Akt ratio in the prostate cancer group was significantly lower than that in the HGPIN group, while there was no significant difference in the p-mTOR ratio between the HGPIN and prostate cancer groups. In the prostate cancer group, no significant relationships were observed between major clinicopathological parameters and the expression levels as well as the ratios of p-Akt or p-mTOR. Conclusions: The Akt-mTOR signaling pathway may play a limited role in the progression of prostate cancer.

10.1159/000356262

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Semen quality changes during infection and recovery phases of mild-to-moderate COVID-19 in reproductive-aged patients: a prospective case series

Basic and Clinical Andrology (2023)

Nasreldin Mohammed Mostafa Kamel Rabea Ahmed Gadelkareem Mohammed Ali Zarzour Adel Kurkar

Despite the documented effects of the coronavirus disease 2019 (COVID-19) on spermatogenesis, the reversibility of these effects is uncertain. We aimed to assess the changes of sperm quality between the infection and recovery phases of COVID-19 in reproductive-aged men. The semen quality of men with mild-to-moderated COVID-19 (defined by the degrees of symptoms and chest involvement on computed tomography) was studied during October, 2020-May, 2021 at our hospital. Two semen samples were analyzed at timings estimated to represent spermatogenic cycles during the infection and recovery phases of COVID-19.A total of 100 patients were included with mean ± SD (range) age of 24.6 ± 3.3 (21-35) years. During infection, 33% of patients had abnormal semen quality. However, a significant reduction was found in this abnormality from 33 to 11% (P < 0.001) after recovery from infection. In a comparison of the two semen analyses, there were significant improvements in the mean values of sperm progressive motility (P =0.043) and normal morphology (P < 0.001). However, the mean sperm concentration showed a statistically insignificant increase (P = 0.844).In reproductive-aged patients with mild-to-moderate COVID-19, the effects on seminal quality were recoverable, represented by significant improvements in the means of progressive sperm motility and normal morphology between the infection and recovery phases of COVID-19.ClinicalTrials, NCT04595240 .RéSUMé: CONTEXTE: Malgré les effets documentés de la maladie à coronavirus 2019 (COVID-19) sur la spermatogenèse, la réversibilité de ces effets reste incertaine. Notre objectif était d’évaluer les changements de la qualité du sperme entre les phases d’infection et de récupération de la COVID-19 chez des hommes en âge de procréer. La qualité du sperme d’hommes atteints d’une forme de COVID-19 de légère à modérée (définie par les degrés de symptômes et l’atteinte thoracique lors de la tomodensitométrie) a été étudiée entre octobre 2020 et mai 2021 dans notre hôpital. Deux échantillons de sperme ont été analysés à des moments estimés représenter les cycles de spermatogénèse pendant les phases d’infection et de rétablissement de la COVID-19. RéSULTATS: Au total, 100 patients ont été inclus avec un âge moyen ± ET (intervalle) de 24,6 ± 3,3 ans (21-35). Au cours de l’infection, 33% des patients avaient une qualité anormale de sperme. Une réduction significative de cette anomalie de 33 à 11% (p < 0,001) a toutefois été observée après la guérison de l’infection. La comparaison des deux analyses de sperme montre des améliorations significatives des valeurs moyennes de la motilité progressive (p = 0,043) et de la morphologie normale (p< 0,001) des spermatozoïdes. Cependant, la concentration moyenne de spermatozoïdes a montré une augmentation statistiquement non significative (p = 0,844). CONCLUSIONS: Chez les patients en âge de procréer atteints d’une forme de COVID-19 légère à modérée, les effets sur la qualité spermatique étaient récupérables, avec des améliorations significatives des valeurs moyennes de la motilité progressive et de la morphologie normale des spermatozoïdes entre les phases d’infection et de récupération de la COVID-19.

Semen quality

Semen Analysis

10.1186/s12610-022-00175-7

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1778 EXPRESSION OF INTEGRIN PROTEINS IN NON-MUSCLE INVASIVE BLADDER CANCER: SIGNIFICANCE OF INTRAVESICAL RECURRENCE FOLLOWING TRANSURETHRAL RESECTION

The Journal of Urology (2012)

Hideaki Miyake Hosny M. Behnsawy Masato Fujisawa

You have accessJournal of UrologyBladder Cancer: Superficial II1 Apr 20121778 EXPRESSION OF INTEGRIN PROTEINS IN NON-MUSCLE INVASIVE BLADDER CANCER: SIGNIFICANCE OF INTRAVESICAL RECURRENCE FOLLOWING TRANSURETHRAL RESECTION Hideaki Miyake, Hosny Behnsawy, and Masato Fujisawa Hideaki MiyakeHideaki Miyake Kobe, Japan More articles by this author , Hosny BehnsawyHosny Behnsawy Kobe, Japan More articles by this author , and Masato FujisawaMasato Fujisawa Kobe, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1795AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Intravesical recurrence after transurethral resection (TUR) has been reported to occur in approximately 50% of patients with non-muscle invasive bladder cancer. Integrins are a family of transmembrane heterodimers, many of which function as receptors for extracellular matrix molecules. To date, a few studies have investigated the role of integrins in bladder cancer progression; therefore, it remains unknown whether intergrins have a significant impact on the prognosis of patients with bladder cancer, particularly that of those with non-muscle invasive disease. The objective of this study was to evaluate the expression levels of integrins in non-muscle invasive bladder cancer to clarify the significance of these proteins as predictors of intravesical recurrence in patients treated with TUR. METHODS This study included 161 patients with non-muscle invasive bladder cancer undergoing TUR between 2000 and 2007. Expression levels of α2, α3, α5, α6, β1 and β4 integrins in TUR specimens obtained from these patients were measured by immunohistochemical staining. RESULTS Of these 6 proteins, expression level of α2, α3, α6 and β4 integrins were significantly associated with several conventional predictive factors of intravesical recurrence. Univariate analysis identified expression level of α3, α6 and β4 integrins as significant predictors of intravesical recurrence, while tumor size, pathological T stage and concomitant carcinoma in situ (CIS) were also significant. Of these significant factors, β4 integrin expression level, pathological T stage and concomitant CIS appeared to be independently related to intravesical recurrence by multivariate analysis. Furthermore, there were significant differences in recurrence-free survival according to positive numbers of these three independent risk factors; that is, intravesical recurrence occurred in 10 of 49 patients who were negative for all risk factors (20.4%), 31 of 68 positive for a single risk factor (45.6%), and 30 of 44 positive for 2 or 3 risk factors (68.2%). CONCLUSIONS Consideration of expression levels of integrins in TUR specimens, in addition to conventional prognostic parameters, would contribute to accurate prediction of intravesical recurrence following TUR for non-muscle invasive bladder cancer. Moreover, combined evaluation of β4 integrin expression, pathological T stage and concomitant CIS would be particularly useful for further refinement of the system in predicting recurrence of non-muscle invasive bladder cancer following TUR. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e718 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.Metrics Author Information Hideaki Miyake Kobe, Japan More articles by this author Hosny Behnsawy Kobe, Japan More articles by this author Masato Fujisawa Kobe, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

Clinical Significance

10.1016/j.juro.2012.02.1795

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Expression patterns of epithelial–mesenchymal transition markers in localized prostate cancer: significance in clinicopathological outcomes following radical prostatectomy

BJU International (2012)

Hosny M. Behnsawy Hideaki Miyake Kenichi Harada Masato Fujisawa

To analyse the expression patterns of multiple molecular markers implicated in epithelial-mesenchymal transition (EMT) in localized prostate cancer (PC), in order to clarify the significance of these markers in patients undergoing radical prostatectomy (RP).Expression levels of 13 EMT markers, namely E-cadherin, N-cadherin, β-catenin, γ-catenin, fibronectin, matrix metalloproteinase (MMP) 2, MMP-9, Slug, Snail, Twist, vimentin, ZEB1 and ZEB2, in RP specimens from 197 consecutive patients with localized PC were evaluated by immunohistochemical staining.Of the 13 markers, expression levels of E-cadherin, Snail, Twist and vimentin were closely associated with several conventional prognostic factors. Univariate analysis identified these four EMT markers as significant predictors for biochemical recurrence (BR), while serum prostate-specific antigen, Gleason score, seminal vesicle invasion (SVI), surgical margin status (SMS) and tumour volume were also significant. Of these significant factors, expression levels of Twist and vimentin, SVI and SMS appeared to be independently related to BR on multivariate analysis. There were significant differences in BR-free survival according to positive numbers of these four independent factors. That is, BR occurred in four of 90 patients who were negative for risk factors (4.4%), 21 of 83 positive for one or two risk factors (25.3%) and 19 of 24 positive for three or four risk factors (79.2%).Measurement of expression levels of potential EMT markers, particularly Twist and vimentin, in RP specimens, in addition to conventional prognostic parameters, would contribute to the accurate prediction of the biochemical outcome in patients with localized PC following RP.

Univariate analysis

Clinical Significance

Biochemical recurrence

10.1111/j.1464-410x.2012.11551.x

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Citations (63)

MP4-18 SIGNIFICANT RECEPTORS OR BIOMARKERS FOR THE SYMPTOMS OF OVERACTIVE BLADDER

The Journal of Urology (2014)

Fukashi Yamamichi Katsumi Shigemura Toshiro Shirakawa Hosny M. Behnsawy Masuo Yamashita

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research II1 Apr 2014MP4-18 SIGNIFICANT RECEPTORS OR BIOMARKERS FOR THE SYMPTOMS OF OVERACTIVE BLADDER Fukashi Yamamichi, Katsumi Shigemura, Toshiro Shirakawa, Hosny Behnsawy, Masuo Yamashita, Hideaki Miyake, Kazushi Tanaka, and Masato Fujisawa Fukashi YamamichiFukashi Yamamichi More articles by this author , Katsumi ShigemuraKatsumi Shigemura More articles by this author , Toshiro ShirakawaToshiro Shirakawa More articles by this author , Hosny BehnsawyHosny Behnsawy More articles by this author , Masuo YamashitaMasuo Yamashita More articles by this author , Hideaki MiyakeHideaki Miyake More articles by this author , Kazushi TanakaKazushi Tanaka More articles by this author , and Masato FujisawaMasato Fujisawa More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.218AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES There were debates as to the pathophysiology of overactive bladder (OAB). The purpose of this study is to investigate significant receptors or biomarkers for overactive bladder (OAB) and OAB-related symptoms. METHODS We examined OAB and control patients with known symptom scores using the Overactive Bladder Symptom Score (OABSS) and International Prostate Symptoms Score (IPSS) / quality of life (QOL) questionnaires. Bladder membrane tissues were investigated by immunohistochemical (IHC) staining using antibodies for adrenoreceptor alpha 1-d (α1-d) and beta-3 (β-3), muscarinic receptor (Muscarine-2 (M-2)), inflammatory or immune-system (Interleukin (IL)-6 and cyclooxygenase-2 (Cox-2)), angiogenesis (CD-31), epithelial-mesenchymal transition (EMT) (E-cadherin), and Caspase-3 apoptosis markers. We investigated the statistical correlation between the expressions of these 3 receptors and 5 markers and these symptom scores. RESULTS OABSS urgency and OABSS total significantly correlated with α1-d and β-3 expressions (p=0.0419 and p=0.0187), respectively, and IPSS intermittency and QOL were significantly related to α1-d and M-2 expressions (p=0.0468 and 0.0428). OABSS incontinence, urgency and total scores significantly correlated with Cox-2, α1-d and β-3 expressions (p=0.0305, p=0.0419 and p=0.0187, respectively). IPSS urgency, straining and intermittency were significantly related to Cox-2, E-cadherin and α1-d expressions (p=0.0344, p=0.0380 and p=0.0468, respectively). QOL was also significantly correlated with CD-31 and M-2 expressions (p=0.0496 and 0.0428, respectively). CONCLUSIONS This study demonstrated the expression of Cox-2 (inflammation) correlated with OABSS incontinence and IPSS straining, α1-d correlated with OABSS urgency and IPSS intermittency, E-cadherin (EMT) correlated with IPSS urgency, CD-31 (angiogenesis) and M-2 were correlated with QOL score and β-3 was correlated with OABSS total scores in OAB patients. These data may help elucidate the pathophysiology of OAB. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e50 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Fukashi Yamamichi More articles by this author Katsumi Shigemura More articles by this author Toshiro Shirakawa More articles by this author Hosny Behnsawy More articles by this author Masuo Yamashita More articles by this author Hideaki Miyake More articles by this author Kazushi Tanaka More articles by this author Masato Fujisawa More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...

Muscarine

10.1016/j.juro.2014.02.218

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