Natália Maria Lanzarini

Fundação Oswaldo Cruz

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Jorlan Fernandes

Fundação Oswaldo Cruz

Marize Pereira Miagostovich

Fundação Oswaldo Cruz

Akira Homma

Fundação Oswaldo Cruz

Marcelo Alves Pinto

Fundação Oswaldo Cruz

Eduardo de Mello Volotão

Fundação Oswaldo Cruz

Gentil Arthur Lins Bentes Mendonça de Vasconcelos

Fundação Oswaldo Cruz

Elba Regina Sampaio de Lemos

Fundação Oswaldo Cruz

Josino Costa Moreira

Fundação Oswaldo Cruz

Camille Ferreira Mannarino

Fundação Oswaldo Cruz

Cooperative Institutions

Fundação Oswaldo Cruz

Faculdades Oswaldo Cruz

Universidade Federal do Rio de Janeiro

Ministério da Saúde

International Olive Council

Universidade Federal Fluminense

Escola Nacional de Saúde Pública

National Council for Scientific and Technological Development

Universidade do Estado do Rio de Janeiro

Universidade Federal de Minas Gerais

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Quantification and molecular characterization of intact rotavirus species A (RVA) in municipal solid waste leachate

Journal of Applied Microbiology (2023)

Natália Maria Lanzarini Camille Ferreira Mannarino Rafaela Marinho Mata Enrico Mendes Saggioro Josino Costa Moreira

Leachate comprises a solid waste decomposition product found fresh in collection trucks or as an effluent in landfills. This study aimed to assess the occurrence, concentrations, and genetic diversity of intact rotavirus species A (RVA) in solid waste leachate.Leachate samples were concentrated by ultracentrifugation, treated with propidium monoazide (PMA), and exposed to LED photolysis. Treated and untread samples were extracted using the QIAamp Fast DNA Stool mini kit, and nucleic acids were screened for RVA employing a Taqman® Real-time PCR. The PMA RT-qPCR method detected RVA in eight out of nine truck samples and in 15.40% (2/13) of the landfill leachate samples. The RVA concentrations in the PMA-treated samples ranged from 4.57 × 103 to 2.15 × 107 genomic copies (GC) 100 mL-1 in truck leachate and from 7.83 × 103 to 1.42 × 104 GC 100 mL-1 in landfill samples. Six truck leachate samples were characterized as RVA VP6 genogroup I2 by partial nucleotide sequencing.The high intact RVA detection rates and concentrations in truck leachate samples indicate potential infectivity and comprise a warning for solid waste collectors concerning hand-to-mouth contact and the splash route.

Florfenicol

10.1093/jambio/lxad146

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Um Breve Histórico sobre o Desenvolvimento de Vacinas

Editora FIOCRUZ eBooks (2021)

Jorlan Fernandes Natália Maria Lanzarini Akira Homma Elba Regina Sampaio de Lemos

10.7476/9786557081075.0002

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Quantification of infectious Human mastadenovirus in environmental matrices using PMAxx-qPCR

Brazilian Journal of Microbiology (2022)

Lorena da Graça Pedrosa de Macena Joseane Simone de Oliveira Pereira Jansen Couto da Silva Fernando César Ferreira Adriana Gonçalves Maranhão

Infectivity

Propidium monoazide

Log reduction

10.1007/s42770-022-00775-5

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Citations (9)

Por Que Vacinamos?

Editora FIOCRUZ eBooks (2021)

Jorlan Fernandes Natália Maria Lanzarini Akira Homma Elba Regina Sampaio de Lemos

10.7476/9786557081075.0004

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Desafios da Vacinação no Século XXI

Editora FIOCRUZ eBooks (2021)

Jorlan Fernandes Natália Maria Lanzarini Akira Homma Elba Regina Sampaio de Lemos

10.7476/9786557081075.0006

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Evaluation of Viral Recovery Methodologies from Solid Waste Landfill Leachate

Food and Environmental Virology (2020)

Natália Maria Lanzarini Rafaela Marinho Mata Enrico Mendes Saggioro Josino Costa Moreira Camille Ferreira Mannarino

10.1007/s12560-020-09431-3

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Production of immunoglobulin y specific for human rotavirus for diagnostic method and immunotherapy

Gentil Vasconcelos Natália Maria Lanzarini Juliana Bohnen Guimarães Alexandre Silva Eduardo de Mello Volotão

10.35259/isi.sact.2013_26914

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Avaliação do método de floculação para recuperação viral de lixiviado de aterro sanitário

Natália Maria Lanzarini Camille Mannarino J. Ferreira de Oliveira Daniele Maia Bila Josino Costa Moreira

10.35259/isi.sact.2018_27087

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Covid-19 e indivíduos cegos e com baixa visão

Benjamin Constant (2021)

Jorlan Fernandes Natália Maria Lanzarini Maria da Glória Souza Almeida Elba Regina Sampaio de Lemos

Visual impairment, which includes the blind and those with low vision, is one of the main reported disabilities, affecting approximately 2.2 million people worldwide, of whom more than two thirds live in low- or middle-income countries. COVID-19 pandemic, caused by SARS-CoV-2, presents itself as a challenging for this vulnerable population, with adverse health impacts and daily adversities, which can be avoided through a more inclusive community planning. Most of these disabled people will recognize the world through touch, using their hands to read, move around and identify the world. In addition to this scenario, the fact that an important portion of those individuals has multiple deficiencies, often also Visual impairment, which includes the blind and those with low vision, is one of the main reported disabilities, affecting approximately 2.2 million people worldwide, of whom more than two thirds live in low- or middle-income countries. COVID-19 pandemic, caused by SARS-CoV-2, presents itself as a challenging for this vulnerable population, with adverse health impacts and daily adversities, which can be avoided through a more inclusive community planning. Most of these disabled people will recognize the world through touch, using their hands to read, move around and identify the world. In addition to this scenario, the fact that an important portion of those individuals has multiple deficiencies, often alsoVisual impairment, which includes the blind and those with low vision, is one of the main reported disabilities, affecting approximately 2.2 million people worldwide, of whom more than two thirds live in low- or middle-income countries. COVID-19 pandemic, caused by SARS-CoV-2, presents itself as a challenging for this vulnerable population, with adverse health impacts and daily adversities, which can be avoided through a more inclusive community planning. Most of these disabled people will recognize the world through touch, using their hands to read, move around and identify the world. In addition to this scenario, the fact that an important portion of those individuals has multiple deficiencies, often also associated with pre-existing comorbidities, with emphasis on diabetes mellitus, one of the main causes of blindness in adults. The current pandemic represents not only a challenge to seek blind and low vision people, but an opportunity for the development of inclusive health strategies.

Visual Impairment

Pandemic

World population

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Apresentação

Editora FIOCRUZ eBooks (2021)

Jorlan Fernandes Natália Maria Lanzarini Akira Homma Elba Regina Sampaio de Lemos

10.7476/9786557081075.0001

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