UPASHANA BHANDARI

Tribhuvan University

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An overview of research on plant based natural dyes in Nepal: scope and challenges

Journal of Agriculture and Natural Resources (2020)

Netra Lal Bhandari Basant Pokhrel UPASHANA BHANDARI Sulakshana Bhattarai Anil Devkota

The worldwide demand of natural dyes is of great interest due to the increased public awareness about the atmospheric and environmental pollution caused by the commercially available synthetic dyes. Nepal being wealthy in flora, would be fine research laboratory land for the plant based natural dyes. Among most of the natural dyes, plant-based dyes/pigments have wide range of applications in fabric, food, drug coloring, therapeutic values and also in solar cells in presence of different mordants. The use of mordant is inevitable during natural dyeing process in order to improve the fastness properties on fabrics, foods and drugs by forming a co-ordination complex with dye. In this article, a short overview of plant based natural dyes extraction applications and their scope and limitations will be discussed with special reference to Nepal. In the present review, the green methods of dye extraction, and dyeing technologies will be discussed, and the research fields based on natural dyes will be explored. Some of the natural dyes has also shown the antimicrobial, antioxidant, antifungal properties and hence are also discussed with biomedical applications.

Mordant

Scope (computer science)

Natural dye

10.3126/janr.v3i2.32328

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Citations (15)

ANTIBIOTIC SUSCEPTIBILITY PATTERN OF ESCHERICHIA COLI ISOLATED FROM CHILDREN WITH URINARY TRACT INFECTION

Asian Journal of Pharmaceutical and Clinical Research (2021)

Yogesh Oli Ganesh Bhandari UPASHANA BHANDARI Sunita Bista AMRIT KUMAR BHATTARAI

Objective: This work aimed to detect the antibiotic susceptibility pattern of Escherichia coli isolated from children, as it is the most predominant pathogen of urinary tract infection (UTI). Methods: About 530 urine samples were collected and tested using the modified Kirby–Bauer disk diffusion method to find the susceptibility pattern of isolated bacteria. Results: Out of a total of 530 samples, 114 (21.50%) showed significant growth. A total of 8 different types of bacteria were isolated from the growth of positive samples. Among the isolates, E. coli 66 (57.8%) was found to be the most predominant organism followed by Klebsiella pneumoniae 18(15.8%), Proteus spp. 10 (8.8%), Staphylococcus aureus 8 (7.0%), Acinetobacter spp. 4 (3.5%), CoNS 4 (3.5%), Enterobacter spp. 2 (1.8%), and Pseudomonas aeruginosa 2 (1.8%). In the present study, out of 66 E. coli, 37 (56.1%) were multidrug-resistant strain. E. coli showed 94.0% resistance to ceftriaxone followed by ceftazidime 86.5% and cefotaxime 70.3%. Imipenem (91.9%) followed by amikacin (89.2%) seems to be the effective drug against UTI causing E. coli in children. Conclusion: Multidrug resistance may possess difficulties with the choice of therapeutic options for the treatment of severe infections.

Amikacin

Klebsiella

10.22159/ajpcr.2021.v14i2.40128

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Citations (3)

IDENTIFICATION AND DETECTION OF BIOFILM PRODUCING STAPHYLOCOCCUS AUREUS AND ITS ANTIBIOGRAM ACTIVITIES

Asian Journal of Pharmaceutical and Clinical Research (2021)

Sapana Sharma UPASHANA BHANDARI Yogesh Oli Ganesh Bhandari Sunita Bista

Objectives: The main aim of this work is to determine the antibiogram profile of biofilm-producing Staphylococcus aureus from various clinical specimens of the patients. Methods: Various bacterial cultures of non-repeated clinical specimens from a total of 3388 patients were determined using standard microbiological and biochemical methods. Results: Out of 3388 only 604 (17.02%) displayed growth positive. A total of 65 (51.58%) S. aureus isolates were recovered, 25 (38.46%) were identified as methicillin-resistant S. aureus (MRSA) by Cefoxitin (30 μg) disk diffusion technique, of which majority were from pus/wound swab 22 (37.29%). The antibiogram of the isolates was analyzed by Kirby-Bauer disk diffusion technique analyzing Linezolid to be the most effective drug with susceptibility of 100% to both MRSA and methicillin-sensitive S. aureus, followed by vancomycin, tigecycline, and tetracycline. In vitro biofilm production by tissue culture plate (TCP) and Congo red agar method detected 52 (80%) and 25 (38.46%) as biofilm producers, respectively. TCP identified 2 (3.07%), 7 (10.76%), and 44 (67.69%) as strongly, moderately, and weakly adherent. About 30.7% of MRSA obtained were positive biofilm producers. The minimum inhibitory concentration value of Oxacillin for S. aureus by agar dilution method ranged from 0.025 μg/mL to 128 μg/mL. Conclusion: This study shows that biofilm production was more in methicillin-resistant strains and displayed a high degree of resistance to almost all groups of antibiotics.

Antibiogram

Tigecycline

Linezolid

Agar diffusion test

10.22159/ajpcr.2021.v14i4.40728

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Citations (1)