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Miguel Ángel Aguilar-Luis

Peruvian University of Applied Sciences

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Juana del Valle-Mendoza

Peruvian University of Applied Sciences

Wilmer Silva-Caso

Peruvian University of Applied Sciences

Luís J. del Valle

Universitat Politècnica de Catalunya

Hugo Carrillo‐Ng

Peruvian University of Applied Sciences

Johanna Martins-Luna

Peruvian University of Applied Sciences

Ronald Aquino-Ortega

Peruvian University of Applied Sciences

Angela Cornejo‐Tapia

Peruvian University of Applied Sciences

Carlos Palomares-Reyes

Peruvian University of Applied Sciences

Yordi Tarazona-Castro

Peruvian University of Applied Sciences

Pablo Weilg

Mercy Medical Center

Cooperative Institutions

Peruvian University of Applied Sciences

Instituto de Investigación Nutricional

Hospital Base Guillermo Almenara Irigoyen

Universidad Peruana Cayetano Heredia

Dirección Regional de Salud del Callao

National University of San Marcos

National University of Cajamarca

Universitat de Barcelona

University of Malaya

Ministry of Health

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First outbreak of Oropouche Fever reported in a non-endemic central-western region of the Peruvian Amazon. Molecular diagnosis and clinical characteristics

International Journal of Infectious Diseases (2018)

Wilmer Silva-Caso Miguel Ángel Aguilar-Luis Fernando Mazulis Carlos Palomares-Reyes Claudia Weilg

Background: Oropouche virus (OROV) is an underreported and emerging infectious disease. Its incidence is underestimated mainly due to clinical similarities with other diseases that are also caused by arboviruses present in endemic areas. We report the first outbreak of OROV in the western region of the Peruvian Amazon in the department of Huanuco, Peru. Methods & Materials: Transversal studie about a outbreak occurred in the region of Huanuco, Peru during July of 2016. Blood samples were taken from 268 patients who presented acute febrile syndrome to be later analyzed for Oropouche Virus via Polymerase Chain Reaction. Results: Of all 268 patients, 46 (17%) cases tested were positive for OROV. the most common symptom reported was headaches with a frequency of 87% (n = 40) followed by myalgias with 76% (n = 35), arthralgias with 65.2% (n = 30), retro-ocular pain 60.8% (n = 28) and hyporexia with 50% (n = 23). Concerning signs and symptoms that may suggest severe OROV infection, 4.3% (n = 2) had low platelet count, 8.6% (n = 4) had intense abdominal pain, and only 2.1% (n = 1) had a presentation with thoracic pain. Conclusion: The disease caused by the OROV is an emerging, underdiagnosed infection that requires ongoing research to determine its virulence, pathogenesis, host range and vectors involved in the urban and sylvatic cycles as well as identifying new genotypes to implement diagnostic tools with appropriate sensitivity, that can be applied to endemic regions. This study reports an outbreak of OROV in a region where it was not previously identified. Even though there have not been any mortalities reported since the initial identification of OROV, recent outbreaks demonstrate a pattern that highlights the emerging potential of the virus and shows the need to prioritize local epidemiological surveillance and the implementation of new public health policies to decrease the health implications that future outbreaks may cause in the population.

10.1016/j.ijid.2018.04.3850

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Citations (2)

Dataset SARS-CoV2-cytokines y2021m02 [50]

Figshare (2021)

Juana del Valle-Mendoza Yordi Qesler Tarazona Castro Alfredo Merino‐Luna Hugo Carrillo‐Ng Hugo Carrillo‐Ng

Comparison of cytokines levels among COVID-19 patients living at sea level and high altitude ABSTRACT Objective: The objective of this study was to compare the levels of pro-inflammatory cytokines (IL-6, IL-2, IL-10, INF-α and IFN-γ) in COVID-19 patients and healthy subjects, residing in two cities of Peru at different altitudes. Results: A total of 35 COVID-19 patients and 10 healthy subjects were recruited from each study site. The mean levels of IL-6 (p<0.03) and TNF-α (p<0.01) were significantly different among the study groups. In the case of IL-6, patients from Lima had a mean level of 16.2 pg/ml (healthy) and 48.3 pg/ml (COVID-19), meanwhile, patients from Huaraz had levels of 67.3 pg/ml (healthy) and 97.9 pg/ml (COVID-19). Regarding TNF-α, patients from Lima had a mean level of 25.9 pg/ml (healthy) and 61.6 pg/ml (COVID-19), meanwhile, patients from Huaraz had levels of 89.0 pg/ml (healthy) and 120.6 pg/ml (COVID-19). The levels of IL-2, IL-10 and IFN-γ) were not significantly different in the study groups.

10.6084/m9.figshare.14050610

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Dataset Microbiota AG y2020m03

Figshare (2020)

Juana del Valle-Mendoza André Taco-Masias Rafael Fernández-Aristi Ángela Cornejo Tapia Pablo Weilg

Gut microbiota in hospitalized children under 5 years with acute infective gastroenteritis caused by virus or bacteria in a regional Peruvian hospital

ABSTRACTObjective: The main objective of this study was to describe the prevalence of 13 representative bacteria from the gut microbiota (GM) in stools samples from children under 5 years of age with acute infective gastroenteritis (AIG) as well as to describe clinical and demographic features from our study population.Results: The most commonly isolated bacteria from the GM were Firmicutes (63.2%) Bacteriodetes (62.4%), Lactobacillus (59.8%), Prevotella (57.3%), Proteobacterium (53.8%), regardless of the etiological agent responsible for the AIG. Interestingly, despite the high prevalence of Firmicutes, Bacteroidetes, Lactobacillus, and Prevotella across all samples, a visible reduction of these agents was observed especially among patients with a single bacterial infection or even bacteria – bacteria coinfections when compared to viral etiologies. Patients with exclusive or mixed breastfeeding registered the highest amount of gut microbiota bacteria, in contrast to infants who received formula or were not breastfed.

10.6084/m9.figshare.12009492

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Detection of human Metapneumovirus infection in children under 18 years old hospitalized in Lima-Peru

PeerJ (2019)

Juana del Valle-Mendoza Fiorella Orellana-Peralta Luís J. del Valle Eduardo Verne Claudia Ugarte

Human Metapneumovirus (hMPV) is a negative single-stranded RNA virus. Infection by hMPV mainly affects the pediatric population and can cause upper or lower respiratory tract pathologies which can develop life threating complications. This study was carried out between 2009 and 2010 in a high complexity national hospital in Lima, Peru. The time frame corresponds to the pandemic of influenza A H1N1.A prospective study was performed between September 2009 and September 2010. Patients with a clinical diagnosis suggestive of an acute respiratory infection were included. RT-PCR was utilized to attain the amplification and identification of the hMPV.A total of 539 samples were analyzed from patients with a clinical context suggestive of an acute respiratory tract infection. Of these samples 73, (13.54%) were positive for hMPV. Out of the positive cases, 63% were under one year old, and increased to nearly 80% when considering children younger than two years old. Cough was the most frequent symptom presented by our population with a number of 62 cases (84.93%). Viral seasonality was also established, noting its predominance during the months of summer in the southern hemisphere. The infection by hMPV has an important prevalence in Peru. It mainly affects children under one year old and should be considered an important differential diagnosis in a patient with an acute respiratory infection.

Metapneumovirus

10.7717/peerj.7266

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Citations (3)

Dataset Microbiota AG y2019m02

Figshare (2020)

Juana del Valle-Mendoza André Taco-Masias Rafael Fernández-Aristi Angela Cornejo‐Tapia Pablo Weilg

10.6084/m9.figshare.7692623

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In Vitro Evaluation of Bacterial Adhesion and Bacterial Viability of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis on the Abutment Surface of Titanium and Zirconium Dental Implants

International Journal of Dentistry (2019)

Ana Stefany Meza-Siccha Miguel Ángel Aguilar-Luis Wilmer Silva-Caso Fernando Mazulis Carolina Barragan-Salazar

Objective . To evaluate the in vitro adherence and viability of 3 bacterial species Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 10556), and Porphyromonas gingivalis (ATCC 33277) on the surfaces of dental implants of titanium, zirconium, and their respective fixing screws. Methods . Two analysis groups were formed: group 1 with 3 titanium pillars and group 2 with 3 zirconium pillars, each with their respective fixing screws. Each of these groups was included in tubes with bacterial cultures of Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 10556), and Porphyromonas gingivalis (ATCC 33277). These samples were incubated at 37°C under anaerobic conditions. Bacterial adherence was assessed by measurement of the change in colony-forming units (CFU), and bacterial viability was evaluated with the colorimetric test of 3-(4,5-dimethylthiazol-2)-2,5 diphenyl tetrazolium bromide (MTT). Results . The bacterial adhesion in the titanium abutments was higher for Streptococcus mutans (190.90 CFU/mL), and the viability was greater in Porphyromonas gingivalis (73.22%). The zirconium abutment group showed the highest adherence with Streptococcus mutans (331.82 CFU/mL) and the highest bacterial viability with the S. sanguinis strain (38.42%). The titanium fixation screws showed the highest adhesion with S. sanguinis (132.5 CFU/mL) compared to the zirconium fixation screws where S. mutans had the highest adhesion (145.5 CFU/mL). The bacterial viability of S. mutans was greater both in the titanium fixation screws and in the zirconium fixation screws 78.04% and 57.38%, respectively. Conclusions . Our results indicate that there is in vitro bacterial adherence and viability in both titanium abutments and zirconium abutments and fixation screws for both. Streptococcus mutans is the microorganism that shows the greatest adherence to the surfaces of both titanium and zirconium and the fixing screws of the latter. On the contrary, bacterial viability is greater on the titanium abutments with P. gingivalis than on the zirconium abutments with S. sanguinis . With respect to the fixation screws, in both cases, the viability of S. mutans was greater with respect to the other bacteria. In general, the titanium abutments showed less adherence but greater bacterial viability.

Streptococcus sanguinis

10.1155/2019/4292976

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The Chikungunya virus: A reemerging cause of acute febrile illness in the high jungle of northern Peru

PLoS neglected tropical diseases (2023)

Miguel Ángel Aguilar-Luis Hugh Watson Yordi Tarazona-Castro Lucinda Troyes-Rivera Felipe Cabellos-Altamirano

Background The Chikungunya virus (CHIKV) is an emerging arthropod-borne virus (arbovirus) that causes undifferentiated acute febrile illness. Cases of CHIKV may be under-reported in Peru, given the various difficulties in diagnosing it, such as lack of diagnostic tests in remote areas, the passive nature of epidemiological surveillance, and co-circulation of other arthropod-borne pathogens. Therefore, a study was conducted in the high jungle of northern Peru to determine the prevalence of CHIKV among febrile patients and describe their clinical characteristics. Methods A cross-sectional study was conducted in the province of Jaen, Cajamarca, located in the high jungle of northern Peru. Patients attending primary healthcare centers within Cajamarca’s Regional Health Directorate were enrolled. The study took place from June 2020 through June 2021. Patients were eligible if they sought outpatient healthcare for a clinical diagnosis of acute febrile illness (AFI). Serum samples were collected from all patients, and the diagnosis of CHIKV was determined using real-time RT-PCR, as well as the detection of IgM antibodies by ELISA. A logistic regression model was employed to identify the risk factors for CHIKV, and the odds ratios (ORs) were calculated, along with their corresponding 95% confidence intervals (95% CI). Results A total of 1 047 patients with AFI were included during the study period. CHIKV was identified in 130 patients of 1 047 (12.4%). Among the CHIKV positive cases, 84 of 130 (64.6%) were diagnosed by RT-PCR, 42 of 130 (32.3%) by IgM ELISA detection, and 4 of 130 (3.1%) by both assays. The majority of patients with CHIKV infection fell within the 18–39 years age group (50.0%), followed by the 40–59 years age group (23.9%) and those with 60 years or older (10.8%). The most common clinical symptoms observed in patients with CHIKV infection were headache (85.4%), myalgias (72.3%), and arthralgias (64.6%). The highest number of positive CHIKV cases occurred in May (23.1%), followed by March (20.0%) and February (13.8%) of 2021. Conclusion The study reports a considerable frequency of CHIKV infections among patients with AFI from the high jungle of northern Peru. These findings highlight the importance of recognizing CHIKV as an ongoing pathogen with continuous transmission in various areas of Peru. It is crucial to enhance epidemiological surveillance by implementing reliable diagnostic techniques, as the clinical symptoms of CHIKV infection can be nonspecific.

10.1371/journal.pntd.0011787

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Citations (1)

Clinical characteristics and molecular detection of Bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru

Iranian Journal of Microbiology (2021)

Juana del Valle-Mendoza Cristina del Valle-Varga Ronald Aquino-Ortega Luís J. del Valle Erico Cieza-Mora

Background and Objectives: Pertussis is an infectious disease caused by the Gram-negative bacterium Bordetella pertussis. In Peru, actual public health programs indicate that vaccination against B. pertussis must be mandatory and generalized, besides all detected cases must be reported. The objective of this study was to determine the prevalence of B. pertussis among children under five years of age with a presumptive diagnosis of whopping cough in Cajamarca, a region located in northern Peru. Materials and Methods: The population of this cross-sectional study were children under 5 years old hospitalized as presumptive cases of pertussis during December 2017 to December 2018. The nasopharyngeal samples were analyzed by real-time PCR for the detection of B. pertussis.

Leukocytosis

Whooping cough

Lymphocytosis

Leukopenia

10.18502/ijm.v13i1.5488

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Citations (1)

Impact of Adding a Rapid PCR-Based Blood Culture Identification Panel to the Antimicrobial Stewardship Program of Patients with Febrile Neutropenia in a Peruvian Referral Hospital

Antibiotics (2023)

Giancarlo Pérez‐Lazo Juana del Valle-Mendoza Roxana Sandoval‐Ahumada Fernando Soto-Febres Raúl Castillo-Córdova

The addition of Biofire® FilmArray® Blood Culture Identification panel 2 (BCID2) to the antimicrobial stewardship program (ASP) could improve outcomes in bloodstream infections (BSI) of patients with febrile neutropenia (FN). A pre- and post-quasi-experimental single-center study was conducted at a reference hospital in Peru. Three groups were considered: patients with BSI before ASP intervention (control group), patients with BSI after ASP intervention (group 1), and patients with BSI after ASP intervention plus BCID2 PCR Panel implementation (group 2). Overall, 93 patients were identified (32 control, 30 group 1, 31 group 2). The median time to effective therapy was significantly shorter in group 2 compared to group 1 and control group, respectively (3.75 vs. 10 h, p = 0.004; 3.75 vs. 19 h, p < 0.001). No significant differences in terms of relapse of bacteremia, in-hospital mortality (all cause), and 30-day-all-cause hospital readmission between the three study periods were found. The appropriateness of empirical antimicrobial use, adding or change, and the following de-escalation or discontinuation was significant when the two intervention periods were compared with the control group (p < 0.001). In addition to the lack of local studies documenting the microbiological profile of FN episodes, adding syndromic panels-based testing could allow for the consolidation of ASP strategies.

Antimicrobial Stewardship

Bacteremia

Blood Culture

Discontinuation

10.3390/antibiotics12040648

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Citations (6)

Clinical characteristics and molecular detection of bordetella pertussis in hospitalized children with a clinical diagnosis of whooping cough in Peru

Zenodo (CERN European Organization for Nuclear Research) (2021)

Juana del Valle-Mendoza Cristina del Valle-Vargas Ronald Aquino-Ortega Luís J. del Valle Erico Cieza-Mora

Whooping cough

10.5281/zenodo.6907934

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