Li Zhou

Chinese Academy of Medical Sciences & Peking Union Medical College

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Tianxin Yang

University of Utah

Zhuan Zhou

Peking University

Hua‐Dong Xu

Chinese Institute for Brain Research

Changhe Wang

Xi'an Jiaotong University

Xinjiang Kang

Affiliated Hospital of Southwest Medical University

Aihua Lu

Sun Yat-sen University

Feipeng Zhu

Chinese Institute for Brain Research

Qihui Wu

Chinese Institute for Brain Research

Panli Zuo

Chinese Institute for Brain Research

Hui Fang

Weifang Medical University

Cooperative Institutions

Chinese Academy of Medical Sciences & Peking Union Medical College

122

Peking University

113

Chinese Academy of Sciences

Center for Life Sciences

Chinese Institute for Brain Research

Sun Yat-sen University

Peking Union Medical College Hospital

Institute of Laboratory Animal Science

University of Chinese Academy of Sciences

Central South University

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Curcumin inhibits renal cyst formation and enlargement in vitro by regulating intracellular signaling pathways

European Journal of Pharmacology (2010)

Jinsheng Gao Hong Zhou Tianluo Lei Li Zhou Weidong Li

10.1016/j.ejphar.2010.12.008

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Citations (55)

A method for recording the two phases of dopamine release in mammalian brain striatum slices

The Analyst (2019)

Ruiying Jiao Wei Liu Lili Yin Zhongjun Qiao Jie Li

Striatal dopamine (DA) release plays an essential role in many physiological functions including motor and non-motor behaviors (such as reward, motivation, and cognition). We have previously reported that, following a single electrical field stimulation, the amperometric recording of DA release from presynaptic terminals in striatal slices (both ventral and dorsal) contains two temporally separated phases. The first phase (direct DA transmission, direct DT) arises from DA terminal release following autologous action potentials (APs), while the second phase (cholinergic transmission-induced DA transmission, CTDT) arises from delayed DA release triggered by the activation of cholinergic interneurons to DA terminals (axon-axon transmission). The millisecond time-resolution of amperometry permits separation of an ∼7 ms latency difference from the single synapse (axon-axon) within the two-phase DA-release (2pDA) signal, and thus the 2pDA signal provides a novel method to study either direct DT, or CTDT, or both. Here, we describe the 2pDA method, including signal recording, processing, analysis, and troubleshooting (anti-artifact). Compared with other DA assays using different stimuli, recording methods, and preparations (such as high performance liquid chromatography or fast scan cyclic voltammetry), 2pDA recording is a novel and powerful physiological recording method for the study of DA transmissions in situ.

10.1039/c9an01941c

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Citations (1)

Study on the Physical Changes of Structure I Methane Hydrate under External Forces

Xianwu Jing Li Zhou Ziyi Fu Houan Zhou

10.1109/oceans51537.2024.10682196

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High potassium promotes mutual interaction between (pro)renin receptor and the local renin-angiotensin-aldosterone system in rat inner medullary collecting duct cells

AJP Cell Physiology (2016)

Chuanming Xu Hui Fang Li Zhou Aihua Lu Tianxin Yang

(Pro)renin receptor (PRR) is predominantly expressed in the collecting duct (CD) with unclear functional implication. It is not known whether CD PRR is regulated by high potassium (HK). Here, we aimed to investigate the effect of HK on PRR expression and its role in regulation of aldosterone synthesis and release in the CD. In primary rat inner medullary CD cells, HK augmented PRR expression and soluble PPR (sPRR) release in a time- and dose-dependent manner, which was attenuated by PRR small interfering RNA (siRNA), eplerenone, and losartan. HK upregulated aldosterone release in parallel with an increase of CYP11B2 (cytochrome P-450, family 11, subfamily B, polypeptide 2) protein expression and upregulation of medium renin activity, both of which were attenuated by a PRR antagonist PRO20, PRR siRNA, eplerenone, and losartan. Similarly, prorenin upregulated aldosterone release and CYP11B2 expression, both of which were attenuated by PRR siRNA. Interestingly, a recombinant sPRR (sPRR-His) also stimulated aldosterone release and CYP11B2 expression. Taken together, we conclude that HK enhances a local renin-angiotensin-aldosterone system (RAAS), leading to increased PRR expression, which in turn amplifies the response of the RAAS, ultimately contributing to heightened aldosterone release.

Eplerenone

Aldosterone synthase

Mineralocorticoid

Losartan Potassium

10.1152/ajpcell.00128.2016

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Citations (15)

Catalytic stability of <I>othro</I>-chloronitrobenzene hydrogenation on Ru-Fe/C catalyst

CHINESE JOURNAL OF CATALYSIS (CHINESE VERSION) (2014)

Xiangsheng XU A. Chen Li Zhou Xiaoqing Li Huizi GU

10.3724/sp.j.1088.2013.20959

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Clinical Features and Treatment Outcomes of Patients With Primary Systemic Anaplastic Large Cell Lymphoma: Report From a Single Institution in China

International Journal of Radiation Oncology*Biology*Physics (2012)

Xuan Zhang Y. Li S. Wang W. Wang J. Jin

Anaplastic large-cell lymphoma

Progression-free survival

10.1016/j.ijrobp.2012.07.1646

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Ligand- and voltage-gated Ca ²⁺ channels differentially regulate the mode of vesicular neuropeptide release in mammalian sensory neurons

Science Signaling (2017)

Yeshi Wang Qihui Wu Meiqin Hu Bin Liu Zuying Chai

Neuropeptides released from dorsal root ganglion (DRG) neurons play essential roles in the neurotransmission of sensory inputs, including those underlying nociception and pathological pain. Neuropeptides are released from intracellular vesicles through two modes: a partial release mode called "kiss-and-run" (KAR) and a full release mode called "full fusion-like" (FFL). Using total internal reflection fluorescence (TIRF) microscopy, we traced the release of pH-sensitive green fluorescent protein-tagged neuropeptide Y (pHluorin-NPY) from individual dense-core vesicles in the soma and axon of single DRG neurons after Ca2+ influx through either voltage-gated Ca2+ channels (VGCCs) or ligand-gated transient receptor potential vanilloid 1 (TRPV1) channels. We found that Ca2+ influx through VGCCs stimulated FFL and a greater single release of neuropeptides. In contrast, Ca2+ influx through TRPV1 channels stimulated KAR and a pulsed but prolonged release of neuropeptides that was partially mediated by Dynamin 1, which limits fusion pore expansion. Suppressing the Ca2+ gradient to an extent similar to that seen after TRPV1 activation abolished the VGCC preference for FFL. The findings suggest that by generating a steeper Ca2+ gradient, VGCCs promote a more robust fusion pore opening that facilitates FFL. Thus, KAR and FFL release modes are differentially regulated by the two principal types of Ca2+-permeable channels in DRG neurons.

Dorsal root ganglion

10.1126/scisignal.aal1683

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Effect of Selective 5-HT6R Agonist on Expression of 5-HT Receptor and Neurotransmitter in Vascular Dementia Rats

Medical Science Monitor (2017)

Haining Yu Tao Chen Li Zhou Jiyou Tang

BACKGROUND 5-HT6 receptor (5-HT6R) has pluripotent roles regulating secretion of neurotransmitters. However, whether 5-HT6R is involved in the development of vascular dementia (VD) remains unclear. To evaluate the role and mechanism of 5-HT6R in VD, this study established a rat VD model to evaluate the effect of selective 5-HT6R agonist on the expression of 5-HT6R mRNA and neurotransmitter. MATERIAL AND METHODS Eighty healthy male SD rats (7 weeks old) were randomly assigned to sham, model, 5-HT6R agonist, and placebo groups (N=20 each). A rat VD model was generated by permeant bilateral ligation of the common carotid artery. 5-HT6R agonist, placebo, or saline were given intraperitoneally for 4 weeks. The Morris water maze was utilized to test learning and memory function. Brains were extracted to separate the cortex and hippocampal tissues, in which glutamate and g-aminobutyric acid (GABA) levels were analyzed. mRNA and protein levels of 5-HT6R were determined by RT-PCR and immunohistochemistry (IHC), respectively. RESULTS Model rats had longer escape latency and fewer crossing platform times. Contents of DA, Glu, GABA, and Ach were lowered in cortical and hippocampal tissues, and 5-HT6R expression was suppressed (p<0.05). The application of 5-HT6R agonist shortened escape latency and increased the number of passing through the platform. It also improved hippocampal CA1 neuronal damage and elevated DA, Glu, GABA, and Ach contents and expression of 5-HT6R. Expression of 5-HT6R was not different from the placebo group. CONCLUSIONS Selective 5-HT6R agonist can alleviate learning deficit of VD rats, possibly via improving neurotransmitter levels in brain regions.

10.12659/msm.899067

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GENETIC DIVERSITY ANALYSIS OF CHINESE THREE-KEELED POND TURTLE (CHINEMYS REEVESII) BY RAPD

Acta Hydrobiologica Sinica (2005)

Xin-Ping ZHU Hejun Du Li Zhou M.-Z. Li Jian‐Fang Gui

Chinese three-keeled pond turtle (Chinemys reevesii Gray) is a member cf the family Emydidae and widely ranges in southern China, especially in the regions of the lower and middle Yangtze River. It has high values for food, traditional Chinese P medicine and pet trade, and is one of the turtles for aquaculture in China. But the genetic background of Chinese three-keeled pond turtle is still not very clear. In order to realize its population genetic structure and the diversity for the conservation of its germ plasm and prevention of gene loss, genetic diversity analysis was carried out using RAPD-PCR technique.In this paper, genetic diversity of 24 individuals of Chinese three-keeled pond turtle from Hubei province was analyzed by the random amplified polymorphic DNA (RAPD) technique, using 20 10-nucleotide-long primers. Twenty primers produced 3288 well-amplified DNA fragments with reproducible banding patterns and averaged 137 DNA fragments in one individual. 119 of 137 loci detected are polymorphic, amounting to 86.9%. The DNA fragments were from 0.2kb to 3kb. The genetic distance between two individuals was from 0.168 to 0.467. The average genetic distance was 0.324 +/- 0.0631 among the 24 individuals. The phylogenetic tree of the 24 individuals was obtained by NJTREE analysis in the RAPDistance program and the 24 individuals were divided into several clusters and it indicated the turtle maybe have two populations at least. In conclusion, genetic diversity of Chinese three-keeled pond turtle was high.

10.3724/issn1000-3207-2005-2-167-r

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Citations (3)

Temporal components of cholinergic terminal to dopaminergic terminal transmission in dorsal striatum slices of mice

The Journal of Physiology (2014)

Li Wang Xiaoyu Zhang Hua‐Dong Xu Li Zhou Ruiying Jiao

Key points The timing of synaptic transmission is critical to synaptic plasticity in the striatum. However, the timing of striatal dopamine (DA) release induced by cholinergic interneurons (ChIs) in the striatum is unclear. In this study, we focused on the temporal components of DA release and replenishment triggered by different pathways. We show that stimulation of ChIs induces DA release with a total delay of 20.6 ms, including 2.8 ms for action potential firing of ChIs, 7.0 ms for cholinergic transmission between acetylcholine terminals and DA terminals, and 10.8 ms for downstream DA release. The delay of DA release via this ChI pathway is 1.9 times that via the nigrostriatal pathways. We describe the time course of recovery of DA release via the two pathways and that of vesicle replenishment in DA terminals. Our work provides an example of unravelling the temporal building blocks during fundamental synaptic terminal–terminal transmission. Abstract Striatal dopamine (DA) is critically involved in major brain functions such as motor control and deficits such as Parkinson's disease. DA is released following stimulation by two pathways: the nigrostriatal pathway and the cholinergic interneuron (ChI) pathway. The timing of synaptic transmission is critical in striatal circuits, because millisecond latency changes can reverse synaptic plasticity from long‐term potentiation to long‐term depression in a DA‐dependent manner. Here, we determined the temporal components of ChI‐driven DA release in striatal slices from optogenetic ChAT‐ChR2‐EYFP mice. After a light stimulus at room temperature, ChIs fired an action potential with a delay of 2.8 ms. The subsequent DA release mediated by nicotinic acetylcholine (ACh) receptors had a total latency of 17.8 ms, comprising 7.0 ms for cholinergic transmission and 10.8 ms for the downstream terminal DA release. Similar latencies of DA release were also found in striatal slices from wild‐type mice. The latency of ChI‐driven DA release was regulated by inhibiting the presynaptic vesicular ACh release. Moreover, we describe the time course of recovery of DA release via the two pathways and that of vesicle replenishment in DA terminals. Our work provides an example of unravelling the temporal building blocks during fundamental synaptic terminal–terminal transmission in motor regulation.

10.1113/jphysiol.2014.271825

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Citations (42)