Thihan Nyun

Cleveland Clinic Lerner College of Medicine

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Stanley M. Hollenberg

Oregon Health & Science University

Tiffani L. Howard

Oregon Health & Science University

Kurt W. Runge

Cleveland Clinic Lerner College of Medicine

Ronald E. Hector

National Center for Agricultural Utilization Research

Kathleen L. Berkner

Cleveland Clinic Lerner College of Medicine

Rebecca L. Shtofman

University of Colorado Denver

Daniel Stauffer

Inova Health System

Alo Ray

Indiana University – Purdue University Indianapolis

Bo-Ruei Chen

Case Western Reserve University

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Cleveland Clinic Lerner College of Medicine

Case Western Reserve University

Cleveland Clinic

Oregon Health & Science University

Vollum Institute

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Fred Hutch Cancer Center

Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa

Hôpital Mongi Slim

Hôpital Lariboisière

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CHMP1 is a novel nuclear matrix protein affecting chromatin structure and cell-cycle progression

Journal of Cell Science (2001)

Daniel Stauffer Tiffani L. Howard Thihan Nyun Stanley M. Hollenberg

The Polycomb-group (PcG) is a diverse set of proteins required for maintenance of gene silencing during development. In a screen for conserved partners of the PcG protein Polycomblike (Pcl), we have identified a new protein, human CHMP1 (CHromatin Modifying Protein; CHarged Multivesicular body Protein), which is encoded by an alternative open reading frame in the PRSM1 gene and is conserved in both complex and simple eukaryotes. CHMP1 contains a predicted bipartite nuclear localization signal and distributes as distinct forms to the cytoplasm and the nuclear matrix in all cell lines tested. We have constructed a stable HEK293 cell line that inducibly overexpresses CHMP1 under ecdysone control. Overexpressed CHMP1 localizes to a punctate subnuclear pattern, encapsulating regions of nuclease-resistant, condensed chromatin. These novel structures are also frequently surrounded by increased histone H3 phosphorylation and acetylation. CHMP1 can recruit a PcG protein, BMI1, to these regions of condensed chromatin and can cooperate with co-expressed vertebrate Pcl in a Xenopus embryo PcG assay; this is consistent with a role in PcG function. In combination, these observations suggest that CHMP1 plays a role in stable gene silencing within the nucleus.

Nuclear matrix

Scaffold/matrix attachment region

Nuclear lamina

10.1242/jcs.114.13.2383

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Tel1p Preferentially Associates with Short Telomeres to Stimulate Their Elongation

Molecular Cell (2007)

Ronald E. Hector Rebecca L. Shtofman Alo Ray Bo-Ruei Chen Thihan Nyun

Elongation

Telomerase RNA component

Elongation factor

10.1016/j.molcel.2007.08.007

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Citations (126)