Jason G Beaudet

Cell Signaling Technology (United States)

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Lana Popova

Cell Signaling Technology (United States)

Sean A. Beausoleil

Cell Signaling Technology (United States)

James S. Wieler

Xencor (United States)

Xiaowu Zhang

China University of Mining and Technology

Ravi Ramenani

Cell Signaling Technology (United States)

Roberto D. Polakiewicz

Cell Signaling Technology (United States)

Sandra M Schieferl

Cell Signaling Technology (United States)

Wan Cheung Cheung

Cell Signaling Technology (United States)

Shuji Sato

Toho University Medical Center Sakura Hospital

A. John Rush

National University of Singapore

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Cell Signaling Technology (United States)

Harvard University

Oregon Health & Science University

Howard Hughes Medical Institute

Second Xiangya Hospital of Central South University

Central South University

Dana-Farber Cancer Institute

Yale University

Muroran Institute of Technology

National University of Singapore

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Monitoring the evolution of antigen-specific antibodies in circulation and their corresponding B cell clones in longitudinal immunization case studies through the use of NG-XMT (P4186)

The Journal of Immunology (2013)

Jaime Darce Stephen Lutz Sandra M Schieferl Elizabeth Castro Moreno Ravi Ramenani

Abstract Previous studies aimed at determining the affinity maturation pathways of monoclonal antibodies during an immunization have relied on comparing germ-line with mature forms of antibodies present in either transgenic B cell receptor mouse models or by cloning circulating human B cells present in peripheral blood (PB) of pre and post-immunized individuals. These approaches have been insightful, but they do not identify in confidence the true B cell clone(s) producing the antigen-specific antibodies found in the sera of immunized animals or humans. We recently developed a technology, NG-XMTTM, encompassing tandem mass spectrometry and next generation sequencing1,2, which for the first time enables the 1) identification of the anatomical location of the B cell clone(s) corresponding to the antigen-specific serum antibodies 2) investigation of clonal diversity of antigen-specific B cell clones residing in primary and secondary lymphoid organs and circulating in PB and 3) analysis of the evolving affinity mature serum antibodies, in two longitudinal immunization studies of rabbits and humans. These results not only advance our understanding of the affinity maturation process and the dynamics of memory B cells and plasma cell development that occurs during an immunization response, they also allow us to use this information to generate useful recombinant antibodies that can be utilized in passive immunization strategies. 1Nat Biotechnol. 2012,30:447; 2Nat Biotechnol. 2012,30:1039

clone (Java method)

10.4049/jimmunol.190.supp.48.3

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Proteomics-directed cloning of circulating antiviral human monoclonal antibodies

Nature Biotechnology (2012)

Shuji Sato Sean A. Beausoleil Lana Popova Jason G Beaudet Ravi Ramenani

Cloning (programming)

10.1038/nbt.2406

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Citations (56)

A proteomics approach for the identification and cloning of monoclonal antibodies from serum