Valeria Grazú

Universidad de Zaragoza

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Jesús M. de la Fuente

Universidad de Zaragoza

100

María Moros

Universidad de Zaragoza

José M. Guisán

Instituto de Catálisis y Petroleoquímica

Roberto Fernández‐Lafuente

Consejo Superior de Investigaciones Científicas

Raluca M. Fratila

Instituto de Nanociencia y Materiales de Aragón

Fernando López‐Gallego

CIC biomaGUNE

César Mateo

Universidad Nacional del Centro de la Provincia de Buenos Aires

Laura Asín

Universidad de Zaragoza

Lucía Gutiérrez

Instituto de Nanociencia y Materiales de Aragón

Lorena Betancor

Universidad ORT Uruguay

Cooperative Institutions

Consejo Superior de Investigaciones Científicas

Universidad de Zaragoza

Instituto de Nanociencia y Materiales de Aragón

Universidad Autónoma de Madrid

Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine

Instituto de Catálisis y Petroleoquímica

Centre National de la Recherche Scientifique

Universitat de Barcelona

Instituto de Salud Carlos III

University of Faisalabad

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Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity

Nano Biomedicine and Engineering (2018)

Paula Sanguiñedo Raluca M. Fratila María Belén Estevez Jesús M. de la Fuente Valeria Grazú

Silver nanoparticles have particular properties that contribute to their very promising applications, novel in various fields of science, such as the development of biosensors, the diagnosis and treatment of cancer, the controlled release of drugs and the antimicrobial potential.The biological synthesis of nanoparticles is of great interest over other physical and chemical methods because the use of toxic chemicals and drastic reaction conditions are avoided.The extracellular biosynthesis using fungi could also make downstream processing much easier than the intracellular biosynthesis.One of the main applications of silver nanoparticles is their antimicrobial activity.Several studies have demonstrated the bactericidal properties of silver nanoparticles are different from silver ions, and that they are strongly influenced by their shape, size, concentration and colloidal state.In the present work, the ability of fungal strains from Uruguay to synthesize silver nanoparticles was studied.Eight fungi were able to synthesize nanoparticles.An extensive physicochemical characterization of the nanoparticles was carried out including ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, zeta-potential and gel electrophoretic mobility.According to the characterization and colloidal stability results, nanoparticles from three fungi were selected for antimicrobial activity assays.All nanoparticles were able to inhibit Escherichia coli growth, demonstrating their potential as effective antibacterial agent for use in biomedical applications.

Extracellular polymeric substance

Silver nanoparticle

10.5101/nbe.v10i2.p165-173

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Citations (31)

Some special features of glyoxyl supports to immobilize proteins

Enzyme and Microbial Technology (2005)

César Mateo Olga Abián Marieta Bernedo Emma Cuenca Manuel Fuentes

Cyanogen bromide

Glutaraldehyde

Agarose

10.1016/j.enzmictec.2005.03.020

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Citations (288)

Use of polyvalent cations to improve the adsorption strength between adsorbed enzymes and supports coated with dextran sulfate

Enzyme and Microbial Technology (2005)

Manuel Fuentes Jorgette V. Maquiese Benevides C. Pessela Rodrigo Torres Valeria Grazú

Thermal Stability

10.1016/j.enzmictec.2005.11.004

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Citations (7)

Glyoxyl agarose: A fully inert and hydrophilic support for immobilization and high stabilization of proteins

Enzyme and Microbial Technology (2005)

César Mateo José M. Palomo Manuel Fuentes Lorena Betancor Valeria Grazú

Agarose

Conjugate

Inert

Borohydride

10.1016/j.enzmictec.2005.10.014

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Citations (372)

Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups

Methods in biotechnology (2006)

César Mateo Olga Abián Gloria Fernández‐Lorente Benevides C. Pessela Valeria Grazú

Commercial epoxy supports may be very useful tools to stabilize proteins via multipoint covalent attachment if the immobilization is properly designed. In this chapter, a protocol to take full advantage of the support's possibilities is described. The basics of the protocol are as follows: (1) the enzymes are hydrophobically adsorbed on the supports at high ionic strength. (2) There is an "intermolecular" covalent reaction between the adsorbed protein and the supports. (3) The immobilized protein is incubated at alkaline pH to increase the multipoint covalent attachment, thereby stabilizing the enzyme. (4) The hydrophobic surface of the support is hydrophylized by reaction of the remaining groups with amino acids in order to reduce the unfavorable enzyme-support hydrophobic interactions. This strategy has produced a significant increase in the stability of penicillin G acylase compared with the stability achieved using conventional protocols.

Hydrophobic effect

10.1007/978-1-59745-053-9_4

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Citations (10)

Promotion of multipoint covalent immobilization through different regions of genetically modified penicillin G acylase from E. coli

Process Biochemistry (2009)

Valeria Grazú Fernando López‐Gallego Tamara Montes Olga Abián Ramón González

Penicillin amidase

Residue (chemistry)

Derivative (finance)

10.1016/j.procbio.2009.10.013

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Citations (60)

Improving immunosensor performance through oriented immobilization of antibodies on carbon nanotube composite surfaces

Biosensors and Bioelectronics (2012)

Sara Puertas María de Gracia Villa Ernest Mendoza Cecilia Jiménez‐Jorquera Jesús M. de la Fuente

Surface Modification

Carbodiimide

Polystyrene

10.1016/j.bios.2012.12.010

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Citations (51)

Engineering biofunctional magnetic nanoparticles for biotechnological applications

Nanoscale (2010)

María Moros Beatriz Pelaz Pilar López‐Larrubia Maria Luisa García‐Martín Valeria Grazú

Synthesis and characterization of magnetic nanoparticles with excellent size control are showed here. Their functionalization using an amphiphilic polymer is also described. This strategy allows the stabilization of magnetic nanoparticles in aqueous solvents and in addition, the polymer shell serves as a platform to incorporate relevant biomolecules, such as poly(ethylene glycol) and a number of carbohydrates. Nanoparticles functionalized with carbohydrates show the ability to avoid unspecific interactions between proteins present in the working medium and the nanoparticles, so can be used as an alternative to poly(ethylene glycol) molecules. Results confirm these nanoparticles as excellent contrast agents for magnetic resonance imaging. Changes in the spin–spin transversal relaxation times of the surrounding water protons due to nanoparticle aggregation demonstrates the bioactivity of these nanoparticles functionalized with carbohydrates. To finish with, nanoparticle toxicity is evaluated by means of MTT assay. The obtained results clearly indicate that these nanoparticles are excellent candidates for their further application in nanomedicine or nanobiotechnology.

Biomolecule

Nanobiotechnology

10.1039/c0nr00104j

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Citations (103)

In vitro efficacy of magnetic hyperthermia in 3D cell culture in collagen gel matrix models

Lilianne Beola Lucía Gutiérrez Jesús M. de la Fuente Valeria Grazú Laura Asín

Resumen del trabajo presentado a la IV Reunion de Jovenes Investigadores en Coloides e Interfases, celebrada en Cordoba del 7 al 9 de febrero de 2018.

Matrix (chemical analysis)

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Química click bioortogonal e hipertermia magnética: una nueva estrategia para la transfección celular

Francisco Javier Idiago López Eduardo Moreno Antolín Jesús M. de la Fuente Valeria Grazú Raluca M. Fratila

Resumen del poster presentado a la 8a Jornada de Jovenes Investigadores (Quimica y Fisica) de Aragon, celebrada en Zaragoza el dia 22 de noviembre de 2018.

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