M. Vimalin Jeyalatha

Xiamen University

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Rapid detection of major Gram-positive pathogens in ocular specimens using a novel fluorescent vancomycin-based probe

Sensors & Diagnostics (2022)

Richa Sharma Rajagopalan Harinee Maxime Klausen M. Vimalin Jeyalatha Muhammed Üçüncü

A novel probe, based on vancomycin and 4-nitrobenzoxadiazole was synthesized and characterized, and used for the rapid and specific detection of Gram positive bacteria – the major pathogens responsible for eye infections in ocular specimens.

Gram

Gram-Positive Bacteria

10.1039/d2sd00061j

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Citations (4)

Tissue remodeling after ocular surface reconstruction with denuded amniotic membrane

Scientific Reports (2018)

Jing Jie Jie Yang Hui He Zheng Jian-lan Wenyan Wang

Abstract Amniotic membrane (AM) has been widely used as a temporary or permanent graft in the treatment of various ocular surface diseases. In this study, we compared the epithelial wound healing and tissue remodeling after ocular surface reconstruction with intact amniotic membrane (iAM) or denuded amniotic membrane (dAM). Partial limbal and bulbar conjunctival removal was performed on New Zealand rabbits followed by transplantation of cryo-preserved human iAM or dAM. In vivo observation showed that the epithelial ingrowth was faster on dAM compared to iAM after AM transplantation. Histological observation showed prominent epithelial stratification and increased goblet cell number on dAM after 2 weeks of follow up. Collagen VII degraded in dAM within 2 weeks, while remained in iAM even after 3 weeks. The number of macrophages and α-SMA positive cells in the stroma of remodelized conjunctiva in the dAM transplantation group was considerably less. In conclusion, dAM facilitates epithelial repopulation and goblet cell differentiation, further reduces inflammation and scar formation during conjunctival and corneal limbal reconstruction.

Goblet cell

Amniotic epithelial cells

Amnion

10.1038/s41598-018-24694-4

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Citations (20)

Construction and application of tissue engineered corneal epithelium from human embryonic stem cells

Investigative Ophthalmology & Visual Science (2019)

Jia He Huimin Sun Shangkun Ou Xin He Zhongyang Zhao

Source

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Citations (0)

Epithelial dysplasia in pterygium postoperative granuloma

Experimental Eye Research (2018)

Guoliang Wang Nuo Dong Yanzi Wang Jing Li Fei Dong

Pterygium

10.1016/j.exer.2018.08.014

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A Novel Tissue-Engineered Corneal Stromal Equivalent Based on Amniotic Membrane and Keratocytes

Investigative Ophthalmology & Visual Science (2019)

Xin Che Han Wu Changkai Jia Huimin Sun Shangkun Ou

Purpose: To investigate a novel strategy in constructing tissue-engineered corneal stromal equivalent based on amniotic membrane and keratocytes. Methods: The ultrathin amniotic membrane (UAM) was laminated, with corneal stromal cells (CSCs) distributed between the space of the layered UAMs. Calcein AM staining was used to evaluate cellular viability, morphology, and arrangement. Immunostaining, qRT-PCR, and Western blot were performed to detect gene and protein expression in keratocytes. Optical coherence tomography visualized the cross sections and thickness of the UAM construction. The microstructure of the CSC-secreted extracellular matrix (ECM) was investigated by scanning electron microscopy and transmission electron microscopy (TEM). To evaluate the feasibility of the multilayer UAM-CSC lamination for surgery, the corneal substitute was used to perform lamellar keratoplasty. Slit lamp microscopy and corneal fluorescein staining were performed in postsurgery observation. Results: The CSCs maintained their keratocyte phenotype and secreted well-organized ECM on the aligned UAM surface. The four-layer UAM-CSC lamination attained half thickness of the human cornea (250 ± 18 μm) after 8 weeks' culture, which also showed promising optimal transparency. In TEM images, the CSC-generated ECM displayed stratified, multilayered lamellae with orthogonal fibril arrangement, which was similar to the human cornea microstructure. Furthermore, the stromal equivalent was successfully preformed in lamellar keratoplasty. Four weeks post surgery, the substitute was well integrated into the recipient cornea and completely epithelialized without myofibroblast differentiation. Conclusions: Our study established a novel 3D biomimetic corneal model to replicate the corneal stromal organization with multilayer UAM, which was capable of promoting the development of corneal stroma–like tissues in vitro, establishing a new avenue for basic research and therapeutic potential.

10.1167/iovs.18-24869

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Citations (26)

Draft Genome Sequence of Mycobacterium tuberculosis Strain SNMICRO 2047-20, Isolated from Intraocular Infection

Microbiology Resource Announcements (2022)

M. Vimalin Jeyalatha Umashankar Vetrivel Rajagopalan Harinee Dhanurekha Lakshmipathy Suganeswari Ganesan

Here, we communicate the draft genome sequence of an ocular Mycobacterium tuberculosis strain (SNMICRO 2047-20) that was isolated from the vitreous fluid of a patient diagnosed with endophthalmitis. The genome sequence was 4,391,538 bp long with 3,898 protein-encoding genes and clustered to the East African-Indian lineage.

Lineage (genetic)

Strain (injury)

Sequence (biology)

Insertion sequence

10.1128/mra.00791-22

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Apoptosis of Corneal Stromal Cells Induced by Telopeptides: An In vitro Study

Ophthalmology Research An International Journal (2014)

M. Vimalin Jeyalatha J. Malathi Prema Padmanabhan Hajib N. Madhavan

Background: Uncontrolled collagen degradation is the characteristics of keratoconus.Degradation of type 1 collagen releases telopeptides.The role of telopeptides on the apoptosis of corneal stromal cells have not been studied so far.Methods: Human primary corneal stromal cells was cultivated and treated with varying concentrations of synthetic telopeptides (3.012µg, 6.125µg, 12.25µg, 12.25µg, 23.5µg, 47µg and 94µg) and incubated for 24 hours, 48 hours and 72 hours.MTT and TUNEL assay was performed following incubation.The difference between the number of viable cells present in the treated and untreated cells were considered for the analysis.Results: Primary corneal stromal cells treated with varying concentrations of synthetic telopeptide at 24 hours and 48 hours had no morphological or apoptotic changes, the viability remained 100%.The percentage viability was altered after 72 hours of incubation with the synthetic telopeptide.47µg/ml, 94µg/ml concentrations of telopeptide showed considerable decrease in the cell viability (p<0.05,t test).

10.9734/or/2014/12138

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Hyperlipidemia induces meibomian gland dysfunction

The Ocular Surface (2019)

Jinghua Bu Yang Wu Xiaoxin Cai Nan Jiang M. Vimalin Jeyalatha

Apolipoprotein E

Meibomian gland

Hyperlipidemia

Rosiglitazone

Immunostaining

10.1016/j.jtos.2019.06.002

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Citations (64)

High-Fat Diet Induces Inflammation of Meibomian Gland

Investigative Ophthalmology & Visual Science (2021)

Jinghua Bu Minjie Zhang Yang Wu Nan Jiang Yuli Guo

To determine if a high-fat diet (HFD) induces meibomian gland (MG) inflammation in mice.Male C57BL/6J mice were fed a standard diet (SD), HFD, or HFD supplemented with the peroxisome proliferator-activated receptor gamma (PPAR-γ) agonist rosiglitazone for various durations. Body weight, blood lipid levels, and eyelid changes were monitored at regular intervals. MG sections were subjected to hematoxylin and eosin staining, LipidTox staining, TUNEL assay, and immunostaining. Quantitative RT-PCR and western blot analyses were performed to detect relative gene expression and signaling pathway activation in MGs.MG acinus accumulated more lipids in the mice fed the HFD. Periglandular CD45-positive and F4/80-positive cell infiltration were more evident in the HFD mice, and they were accompanied by upregulation of inflammation-related cytokines. PPAR-γ downregulation accompanied activation of the mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways in the HFD mice. There was increased acini cell apoptosis and mitochondria damage in mice fed the HFD. MG inflammation was ameliorated following a shift to the standard diet and rosiglitazone treatment in the mice fed the HFD.HFD-induced declines in PPAR-γ expression and MAPK and NF-κB signaling pathway activation resulted in MG inflammation and dysfunction in mice.

Meibomian gland

10.1167/iovs.62.10.13

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Citations (31)

Anticancer effects of one-pot synthesized biogenic gold nanoparticles (Mc-AuNps) against laryngeal carcinoma

Journal of Drug Delivery Science and Technology (2017)

Pachaiyappan Saravana Kumar M. Vimalin Jeyalatha J. Malathi S. Ignacimuthu

Zeta potential

10.1016/j.jddst.2017.12.008

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Citations (35)