Rosa María Bermúdez‐Cruz

Center for Research and Advanced Studies of the National Polytechnic Institute

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María Luisa Bazán‐Tejeda

Instituto Politécnico Nacional

Cecilia Montáñez

Instituto Politécnico Nacional

Guadalupe Ortega‐Pierres

Center for Research and Advanced Studies of the National Polytechnic Institute

Francisco Alejandro Lagunas‐Rangel

Uppsala University

Raúl Argüello-García

Instituto Politécnico Nacional

Rocío Fonseca-Liñán

Center for Research and Advanced Studies of the National Polytechnic Institute

Ana Laura Torres-Huerta

Tecnológico de Monterrey

Rosa María Martínez-Miguel

Instituto Politécnico Nacional

José Díaz‐Chávez

Instituto Nacional de Cancerología

Fernando Fernández‐Ramírez

Hospital General de México

Cooperative Institutions

Center for Research and Advanced Studies of the National Polytechnic Institute

Instituto Politécnico Nacional

Universidad Nacional Autónoma de México

Centro de Investigación en Materiales Avanzados

Instituto Nacional de Cancerología

Mexican Social Security Institute

Tecnológico Nacional de México

National Cancer Institute

Frederick National Laboratory for Cancer Research

Instituto de Estudios Avanzados

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Effect of λ λ tI Point Mutations on T3 RNA Polymerase-Mediated Transcription Termination

Revista latinoamericana de microbiología (2000)

Bulmaro Cisneros Rosa María Bermúdez‐Cruz Alejandra Sánchez Cecilia Cecilia

The terminator tI, located approximately 280 nucleotides beyond the int gene of bacteri o- phage lambda, is considered an intrinsic terminator due to its GC-rich region of dyad symmetry followed by a T stretch. In order to study the effect of tI structure in T3 RNA polymerase-mediated transcription termination, three different point mutations tI1, tI2 and tI3 were analyzed. DNA fragments containing the tI mutations were cloned into the pBS+ vector for in vitro transcription studies. All the tI mutations map- ping in the tI stem-loop structure decrease the in vitro transcription termination of tI from 85% for the wild type terminator to 46-60% using the T3 RNA polymerase.

Terminator (solar)

Transcription

RNA polymerase II

RNA polymerase III

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Citations (1)

Early antitermination in the atypical coliphage mEp021 mediated by the Gp17 protein

Archives of Virology (2023)

Guadalupe Valencia-Toxqui Elissa P. Ballinas-Turrén Rosa María Bermúdez‐Cruz Eva Martínez-Peñafiel Gabriel Guarneros

Antitermination

Terminator (solar)

Transcription

10.1007/s00705-023-05721-w

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Citations (1)

Effects of Ionizing Radiation on Embryos of the Tardigrade Milnesium cf. tardigradum at Different Stages of Development

PLoS ONE (2013)

Eliana Beltrán-Pardo Kjell Jonsson Andrzej Wójcik Siamak Haghdoost Mats Harms‐Ringdahl

Tardigrades represent one of the most desiccation and radiation tolerant animals on Earth, and several studies have documented their tolerance in the adult stage. Studies on tolerance during embryological stages are rare, but differential effects of desiccation and freezing on different developmental stages have been reported, as well as dose-dependent effect of gamma irradiation on tardigrade embryos. Here, we report a study evaluating the tolerance of eggs from the eutardigrade Milnesium cf. tardigradum to three doses of gamma radiation (50, 200 and 500 Gy) at the early, middle, and late stage of development. We found that embryos of the middle and late developmental stages were tolerant to all doses, while eggs in the early developmental stage were tolerant only to a dose of 50 Gy, and showed a declining survival with higher dose. We also observed a delay in development of irradiated eggs, suggesting that periods of DNA repair might have taken place after irradiation induced damage. The delay was independent of dose for eggs irradiated in the middle and late stage, possibly indicating a fixed developmental schedule for repair after induced damage. These results show that the tolerance to radiation in tardigrade eggs changes in the course of their development. The mechanisms behind this pattern are unknown, but may relate to changes in mitotic activities over the embryogenesis and/or to activation of response mechanisms to damaged DNA in the course of development.

Tardigrade

Gamma Irradiation

Developmental stage

Radiosensitivity

Cryptobiosis

10.1371/journal.pone.0072098

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Citations (35)

Natural Compounds That Target DNA Repair Pathways and Their Therapeutic Potential to Counteract Cancer Cells

Frontiers in Oncology (2020)

Francisco Alejandro Lagunas‐Rangel Rosa María Bermúdez‐Cruz

Resistance to current cancer treatments is an important problem that arises through various mechanisms, but one that stands out involves an overexpression of several factors associated with DNA repair. To counteract this type of resistance, different drugs have been developed to affect one or more DNA repair pathways, therefore, to test different compounds of natural origin that have been shown to induce cell death in cancer cells is paramount. Since natural compounds target components of the DNA repair pathways, they have been shown to promote cancer cells to be resensitized to current treatments. For this and other reasons, natural compounds have aroused great curiosity and several research projects are being developed around the world to establish combined treatments between them and radio or chemotherapy. In this work, we summarize the effects of different natural compounds on the DNA repair mechanisms of cancer cells and emphasize their possible application to re-sensitize these cells.

10.3389/fonc.2020.598174

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Citations (35)

Nucleic acid and protein factors involved in Escherichia coli polynucleotide phosphorylase function on RNA

Biochimie (2010)

Fernando Fernández‐Ramírez Rosa María Bermúdez‐Cruz Cecilia Montáñez

Polynucleotide phosphorylase

Degradosome

Exoribonuclease

10.1016/j.biochi.2010.01.004

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Citations (13)

Nicotinamide induces G2 cell cycle arrest in Giardia duodenalis trophozoites and promotes changes in sirtuins transcriptional expression

Experimental Parasitology (2019)

Francisco Alejandro Lagunas‐Rangel María Luisa Bazán‐Tejeda Enrique García‐Villa Rosa María Bermúdez‐Cruz

Giardia

Giardia lamblia

10.1016/j.exppara.2019.107822

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Citations (10)

Nus A is involved in transcriptional termination on λ tI

Biochimie (1999)

Rosa María Bermúdez‐Cruz Michael J. Chamberlin Cecilia Montañez

Terminator (solar)

Lambda phage

Transcription

Wild type

Dot blot

10.1016/s0300-9084(99)80134-5

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Citations (10)

MR (Mre11-Rad50) complex in Giardia duodenalis: In vitro characterization and its response upon DNA damage

Biochimie (2015)

Antonio Sandoval-Cabrera Ana L. Zarzosa-Álvarez Rosa María Martínez-Miguel Rosa María Bermúdez‐Cruz

Rad50

10.1016/j.biochi.2015.01.008

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Citations (7)

Giardia duodenalis Rad52 protein: biochemical characterization and response upon DNA damage

The Journal of Biochemistry (2017)

Rosa María Martínez-Miguel Antonio Sandoval-Cabrera María Luisa Bazán‐Tejeda Ana Laura Torres-Huerta Diego A. Martínez-Reyes

Giardia duodenalis is a flagellated binucleated protozoan that colonizes the small intestine in mammals, causing giardiasis, acute or chronic diarrhea. DNA double strand break either endogenously or exogenously generated is a major insult to DNA and its repair by homologous recombination (HR) is crucial for genomic stability. During HR, Rad52 plays key roles in the loading of the Rad51 recombinase, and the annealing of the second double-strand break end to the displaced strand of the D-loop structure. Among the functions found in vitro in yeast and human Rad52 protein are: ssDNA or dsDNA binding activity, ability to anneal bare or RPA coated-ssDNA, as well as multimeric ring formation. In this work, we searched for conserved domains in a putative Rad52 protein from G. duodenalis (GdRad52). Its coding sequence was cloned, expressed and purified to study its biochemical properties. rGdRad52 binds to dsDNA and ssDNA, with greater affinity for the latter. Likewise, rGdRad52 promotes annealing of DNA uncoated and coated with GdRPA1. rGdRad52 interacts with GdDMC1B and with GdRPA1 protein as shown in far western blotting assay. Additionally, rGdRad52 formed multimeric rings as observed by electronic microscopy. Finally, GdRad52 is over expressed in response upon DNA damage inflicted on trophozoites.

RAD52

Replication protein A

10.1093/jb/mvx009

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Citations (4)

Multiplex PCR to detect four different tomato-infecting pathogens

Folia Microbiologica (2012)

Gabriela Alejandra Quintero–Vásquez María Luisa Bazán‐Tejeda Eva Martínez-Peñafiel Luis Kameyama-Kawabe Rosa María Bermúdez‐Cruz

Clavibacter michiganensis

Multiplex

Primer (cosmetics)

10.1007/s12223-012-0206-6

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Citations (7)