Robin L. Brigmon

Savannah River National Laboratory

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Grażyna Płaza

Institute For Ecology of Industrial Areas

Christopher J. Berry

Savannah River National Laboratory

Roger J. Narayan

North Carolina State University

Krzysztof Ulfig

Institute For Ecology of Industrial Areas

Stephen G. Zam

AgResearch

Penny A. Morris

Allan Hancock College

Gabriel Bitton

University of Florida

Alex Kugler

Savannah River National Laboratory

Courtney Burckhalter

Savannah River National Laboratory

C.B. Fliermans

Savannah River National Laboratory

Cooperative Institutions

Savannah River National Laboratory

Clemson University

University of Florida

University of Georgia

Pontificia Universidad Javeriana

Institute For Ecology of Industrial Areas

Westinghouse Electric (United States)

University of South Carolina Aiken

Environmental Protection Agency

Office of Scientific and Technical Information

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Utilization of lasso peptides for biodegradation of polycyclic aromatic hydrocarbons

Environmental Microbiology Reports (2024)

W A Simpson Robin L. Brigmon Daniel L. Howard Makaela Jackson Alex Kugler

Abstract Many microbial genes involved in degrading recalcitrant environmental contaminants such as polycyclic aromatic hydrocarbons (PAHs) have been identified and characterized. However, all molecular mechanisms required for PAH utilization have not yet been elucidated. In this work, we demonstrate the proposed involvement of lasso peptides in the utilization of the PAH phenanthrene in Sphingomonas BPH. Transpositional mutagenesis of Sphingomonas BPH with the miniTn5 transposon yielded 3 phenanthrene utilization deficient mutants, #257, #1778, and #1782. In mutant #1782, Tn5 had inserted into the large subunit of the naph/bph dioxygenase gene. In mutant #1778, Tn5 had inserted into the B2 protease gene of a lasso peptide cluster. This finding is the first report on the role of lasso peptides in PAH utilization. Our studies also demonstrate that interruption of the lasso peptide cluster resulted in a significant increase in the amount of biosurfactant produced in the presence of glucose when compared to the wild‐type strain. Collectively, these results suggest that the mechanisms Sphingomonas BPH utilizes to degrade phenanthrene are far more complex than previously understood and that the #1778 mutant may be a good candidate for bioremediation when glucose is applied as an amendment due to its higher biosurfactant production.

Sphingomonas

Transposon mutagenesis

Lasso

10.1111/1758-2229.13197

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Biofouling of Groundwater Systems by Thiothrix spp.

Current Microbiology (1997)

Robin L. Brigmon H. W. Martin Henry C. Aldrich

Biofouling

10.1007/s002849900233

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Citations (23)

Methanotrophic Bacteria: Use in Bioremediation

Encyclopedia of Environmental Microbiology (2003)

Robin L. Brigmon

Abstract Background In Situ Bioremediation Monitored Natural Attenuation Ex Situ: Bioreactors

10.1002/0471263397.env252

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Citations (14)

Carbonate Biogenic Structures in Storrs Lake, Bahamas

M. Byrne Penny A. Morris Susan J. Wentworth Robin L. Brigmon D. S. McKay

Storr's Lake, an inland hypersaline lake on San Salvador Island, Bahamas, contains calcium carbonate-rich lithified mats of filamentous microorganisms, diatoms, associated photosynthetic and chemotrophic bacteria, and trapped sediment. In addition, 16S rRNA analysis indicates the presence of five sulfur-reducing genera of bacteria. These microbes are potential modern-day analogs to some ancient stromatolitic structures. The goals of this study are to identify unique compositional and biogenic features, possibly correlating some of these with some of the sulfate-reducing bacteria. Additional information is contained in the original extended abstract.

Green sulfur bacteria

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L-Basin microbial monitoring program - evaluation of 20 years of monitoring

Robin L. Brigmon Yuriy Veytskin Courney Burckhalter

mitigate any detrimental impact. This report reviews the microbial monitoring activities since initial characterizations in the mid-1990s.

10.2172/1772364

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Draft Genome Sequence of Cupriavidus basilensis SRS, a Bacterium Isolated from Stream Sediments

OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (2022)

He Fu Alex Kugler Patrick Huyck Robin L. Brigmon Elizabeth A. Ottesen

Sequence (biology)

10.1128/mra.00691-22

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BIOTIGER, A NATURAL MICROBIAL PRODUCT FOR ENHANCED HYDROCARBON RECOVERY FROM OIL SANDS.

Robin L. Brigmon T Topher Berry William W. Jones C Charles Milliken

BioTiger{trademark} is a unique microbial consortia that resulted from over 8 years of extensive microbiology screening and characterization of samples collected from a century-old Polish waste lagoon. BioTiger{trademark} shows rapid and complete degradation of aliphatic and aromatic hydrocarbons, produces novel surfactants, is tolerant of both chemical and metal toxicity and shows good activity at temperature and pH extremes. Although originally developed and used by the U.S. Department of Energy for bioremediation of oil-contaminated soils, recent efforts have proven that BioTiger{trademark} can also be used to increase hydrocarbon recovery from oil sands. This enhanced ex situ oil recovery process utilizes BioTiger{trademark} to optimize bitumen separation. A floatation test protocol with oil sands from Ft. McMurray, Canada was used for the BioTiger{trademark} evaluation. A comparison of hot water extraction/floatation test of the oil sands performed with BioTiger{trademark} demonstrated a 50% improvement in separation as measured by gravimetric analysis in 4 h and a five-fold increase at 25 hr. Since BioTiger{trademark} performs well at high temperatures and process engineering can enhance and sustain metabolic activity, it can be applied to enhance recovery of hydrocarbons from oil sands or other complex recalcitrant matrices.

Human decontamination

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Metagenomic Analysis of Three Samples from the MCU Process

Robin L. Brigmon Charles E. Turick Courtney Burckhalter

Three Modular Caustic-Side Solvent Extraction (CSSX) Unit (MCU) Strip Effluent Feed samples were sent from Savannah River National Laboratory (SRNL) to Microbial Insights (MI), Knoxville, TN, for molecular analysis. The objective of the analysis was to profile and identify dominant members of the microbial community in these samples. More effective control of microbial growth in the MCU depends on the fundamental understanding of the microorganisms responsible for system colonization that leads to biofilm formation and coalescer impairment. This analysis entailed Next-generation sequencing (NGS) of DNA, a collective term for advanced technologies that allow the determination of microbial species present. NGS, also commonly called metagenomics, provides for the identification of microorganisms down to the genus level. Although function, e.g. biofilm formation or corrosion, cannot always be predicted from identification, knowledge of the microorganisms present can offer insight into the potential microbial processes that may influence system function occurring in the MCU. Information on microbial identification can also influence control measures, e.g. operational/maintenance parameters or the choice of biocides to employ. This knowledge can help control undesired microbial activity as conventional cleaning and disinfection regimens may also contribute to inefficient biofilm control and dissemination of microbial resistance to remediation efforts.

Identification

Biocide

10.2172/1508736

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Bioremediation of heavy oily sludge: a microcosms study

Biodegradation (2022)

Cinthya Rondon-Afanador Gustavo Pinilla-Meza Francy C. Casallas-Cuervo Camila Diaz-Vanegas Daniela Barreto-Gomez

Oily sludge is a residue from the petroleum industry composed of a mixture of sand, water, metals, and high content of hydrocarbons (HCs). The heavy oily sludge used in this study originated from Colombian crude oil with high density and low American Petroleum Institute (API) gravity. The residual waste from heavy oil processing was subject to thermal and centrifugal extraction, resulting in heavy oily sludge with very high density and viscosity. Biodegradation of the total petroleum hydrocarbons (TPH) was tested in microcosms using several bioremediation approaches, including: biostimulation with bulking agents and nutrients, the surfactant Tween 80, and bioaugmentation. Select HC degrading bacteria were isolated based on their ability to grow and produce clear zones on different HCs. Degradation of TPH in the microcosms was monitored gravimetrically and with gas chromatography (GC). The TPH removal in all treatments ranged between 2 and 67%, regardless of the addition of microbial consortiums, amendments, or surfactants within the tested parameters. The results of this study demonstrated that bioremediation of heavy oily sludge presents greater challenges to achieve regulatory requirements. Additional physicochemical treatments analysis to remediate this recalcitrant material may be required to achieve a desirable degradation rate.

Bioaugmentation

Biostimulation

Microcosm

Oil sludge

Microbial consortium

10.1007/s10532-022-10006-1

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Citations (12)

Terrestrial Evaporite Environments: Potential Analogs for Mars

LPI (2002)

Penny A. Morris S. J. Wentworth M. Byrne Robin L. Brigmon C. C. Allen

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