V. Ya. Gergert

Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences

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Vladimir Yeremeev

Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences

S. S. Babayan

Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences

АЭ

А. Э. Эргешов

Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences

ВГ

В. Г. Авдиенко

Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences

R.Y.U. Romanova

Ministry of Health of the Russian Federation

ВИ

В. И. Литвинов

National University of Life and Environmental Sciences of Ukraine

Natalya Karpina

Central Research Institute

ММ

М. М. Авербах

Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences

Irina V. Kozlova

State Research Center for Dermatovenereology and Cosmetology

Galina Shepelkova

Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences

Cooperative Institutions

Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences

Russian Academy of Sciences

Ministry of Health of the Russian Federation

Central Research Institute

A N Bach Institute of Biochemistry

Federal Center Research Fundamentals of Biotechnology

Academy of Medical Sciences

Biotechnology Research Center

Institute of Bioorganic Chemistry

Saint-Petersburg Research Institute of Phthisiopulmonology

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DURATION OF IMMUNE RESPONSE INDUCED BY THE VACCINE BASED ON RECOMBINANT Ag85, TB10 AND FliC PROTEINS

Medical Immunology (Russia) (2018)

Vladimir Yeremeev И. В. Духовлинов А. И. Орлов Galina Shepelkova Е. А. Федорова

Already about one hundred years BCG remains the only widely used tuberculosis (TB) vaccine. It is known that intensity of the BCG-induced Th1 immune response decreases over time and comes to naught within 10-15 years. It significantly distinguishes BCG from the vaccines providing a lifelong protection such as vaccines against poliomyelitis or measles, and can be bound to natural restriction of duration of a persistence of the BCG-induced CD4+ T-cells. Data on insufficient ability of BCG to stimulate life-long immunological memory is accumulating. Earlier in our lab on the model of rather resistant to TB C57BL/6 mice infection with the virulent laboratory strain of Mycobacterium tuberculosis (Mtb) H37Rv protective activity (comparable to that of BCG Russia) of 3 subunit vaccine variants was demonstrated as assessed by lung and spleen CFU counts and life span of animals after infection. The aim of this study was to study the characteristics and duration of the immune response induced by the most effective variant of these vaccines. Groups of C57BL/6 mice were vaccinated intramuscularly twice with two-week intervals with 10 μg protein conjugated to 200 μl of an aluminum hydroxide emulsion. Immune response (production of specific antibodies, vaccine proteinstimulated production of interferon gamma and proliferation in vitro) was monitored during 10 months after vaccination. We have shown that the test vaccine induces in mice the formation of long-term immunological memory to a bacterial antigen. Moreover, in the presence of glutoxim the immunological memory spectrum shifts to a "protective" type, i.e. the predominance of the cellular component of the immune response over the antibody response is stimulated. The next step will be the investigation of vaccine effectiveness for revaccination after primary BCG immunization.

Tuberculosis vaccines

BCG vaccine

10.15789/1563-0625-2018-2-271-276

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Citations (1)

PREVENTION OF TUBERCULOSIS: CURRENT APPROACHES TO DEVELOPMENT OF VACCINES

Annals of the Russian academy of medical sciences (2012)

Marina Stukova Natalia Zabolotnykh Т. И. Виноградова V. Ya. Gergert Alexander Apt

This review is focused on recent advances in development of new vaccines for the prevention of tuberculosis. The main reasons for lack of BCG vaccine efficacy in different populations and geographic regions are presented. Design of new vaccines based on live modified strains of Mycobacterium bovis BCG, attenuated strains of Mycobacterium tuberculosis, recombinant proteins and viral vectors is considered in the specific examples. The usage of the heterologous «prime-boost» vaccination strategy against tuberculosis is discussed.

Heterologous

Tuberculosis vaccines

BCG vaccine

Attenuated vaccine

10.15690/vramn.v67i11.471

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Citations (2)

An M. Boms BCG cell wall glicopeptid — EIA for antituberculosis antibodies detection

Tubercle and Lung Disease (1994)

R.Y.U. Romanova V. Ya. Gergert L.A. Gorelova A G Khomenko

10.1016/0962-8479(94)90828-1

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ON THE QUESTION OF INTERPRETATION OF TB IMMUNODIAGNOSTICS RESULTS

Вестник ЦНИИТ (2024)

V. Ya. Gergert

This article was prepared in response to increasing difficulties encountered by phthisiologists in the diagnostics of TB or latent TB infection (LTI) using immunological methods. The author with over 50-year experience in the immunology of infection tried to explain the causes of negative results of different immunological reactions (skin, serological, or cellular immunity reactions) based on his own experience and literature data. Errors in the interpretation of specific tests due objective reasons associated with genetic traits of a microorganism were described. A complex approach to both choice of immunological tests and use of different antigenic stimuli was recommended to increase the accuracy of immunodiagnostics results.

Cellular immunity

10.57014/2587-6678-2024-8-1-5-9

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Efficacy of nucleotide biocorrector derived from baking yeast of the Saccharomyces cerevis genus in patients with lung tuberculosis

PULMONOLOGIYA (2007)

Elena V. Orlova В. С. Орлова V. Ya. Gergert

There is information on efficacy of immunostimulating agents as a part of therapy of lung tuberculosis. Nucleotide biocorrector is a mixture of nucleotides and nucleosides derived from baking yeast of the Saccharomyces cerevis genus and presenting wide immunomodulating properties. The aim of this study was to investigate efficacy of this agent along with combined chemotherapy in 52 patients with destructive lung tuberculosis. Of them, 27 patients were treated with nucleotide biocorrector (3 g per day orally during 1 month) and conventional chemotherapeutic medication (the study group) and 25 patients (the control group) received equal chemotherapy alone. All the patients were MBT-positive. Immunological examination (CD3+, CD4+, CD8+, СD16+, and CD20+ lymphocytes and their functional activity, anti-MBT-antibodies) was done before and 1 month after starting the treatment; clinical, radiological and laboratory investigations were performed at the same time and additionally in 3 month. Addition of biocorrector to the standard chemotherapy of lung tuberculosis allowed faster normalization of CD3+, CD4+ and CD8+ lymphocytes, enhanced functional activity of T-cell immunity and increased number of CD20+ B-lymphocytes. Moreover, this therapy has facilitated resolution of lung infiltration, eradication of MBT and recovery of tuberculous cavities by the 3rd month of the treatment. Tolerability of biocorrector was good.

Tolerability

10.18093/0869-0189-2007-0-1-107-110

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Citations (1)

Nucleic acid synthesis after experimental BCG vaccination as shown by histoautoradiography

Bulletin of Experimental Biology and Medicine (1969)

М. М. Авербах V. Ya. Gergert В. И. Литвинов

BCG vaccine

10.1007/bf00803511

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Differential diagnosis of idiopathic pulmonary fibrosis

Terapevticheskii arkhiv (2020)

Е. И. Шмелев А. Э. Эргешов V. Ya. Gergert

The review is devoted to the urgent problem of modern pulmonology: the differential diagnosis of idiopathic pulmonary fibrosis (ILF). ILF occupies a special place among many interstitial lung diseases for a number of reasons: 1) it is a deadly disease; 2) early diagnosis and adequate antifibrotic therapy significantly extend the life expectancy of patients; 3) anti-inflammatory drugs (corticosteroids) and cytostatics with ILF that are widely used in other forms of interstitial lung diseases are ineffective and accelerate the progression of the process; 4) the commonality of the main clinical signs (increasing respiratory failure) of various interstitial lung diseases. The list of respiratory diseases with which ILF should be differentiated is huge, and if with diffuse lung lesions of a known nature (disseminated pulmonary tuberculosis, pneumoconiosis, etc.) with a certain experience/qualification, the diagnosis is relatively simple, then the isolation of ILF from the group of idiopathic interstitial pneumonias always represents certain difficulties. The main methods used in the diagnosis of ILF are summarized taking into account current international and national recommendations.

Pulmonology

Pneumoconiosis

10.26442/00403660.2020.03.000346

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A Mechanism of Gold Nanoparticle Aggregation by Immunoglobulin G Preparation

Applied Sciences (2020)

Dmitriy V. Sotnikov Irina V. Safenkova Anatoly V. Zherdev В. Г. Авдиенко Irina V. Kozlova

Conjugates of gold nanoparticles (GNPs) and antibodies are widely used in various fields of biochemistry and microbiology. However, the procedure for obtaining such conjugates remains precarious, and the properties of conjugates differ significantly for different antibody clones. One of the most common problems is the aggregation of GNPs in the course of their conjugation with antibodies. This article considers an example of the conjugation of monoclonal antibodies with non-stable aggregating product. The composition of the antibody preparation was studied using electrophoresis, asymmetrical flow field-flow fractionation, and ultracentrifugation. It was shown that the component that causes the aggregation of the GNPs is the light chains of immunoglobulins that appear due to the spontaneous decay of the antibodies. After separation of the fraction with a molecular weight of less than 30 kDa, stable conjugates of antibodies with GNPs were obtained. The high functional activity of the obtained conjugates was confirmed by immunochromatography.

Conjugate

Analytical Ultracentrifugation

10.3390/app10020475

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Citations (10)

Natural killer cells in patients with pulmonary tuberculosis

Bulletin of Experimental Biology and Medicine (1991)

I. S. Khomenko V. Ya. Gergert N. S. Kolodyazhnaya

10.1007/bf00841157

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Comparative performance of QuantiFERON-TB Gold versus skin test with tuberculosis recombinant allergen (Diaskintest) among patients with suspected pulmonary tuberculosis in Russia

International Journal of Infectious Diseases (2019)

Irina Nikitina Natalya Karpina Olga V. Kasimceva V. Ya. Gergert А. Э. Эргешов

The early identification of Mycobacterium tuberculosis infection can prevent tuberculosis (TB) transmission. A skin test with a tuberculosis recombinant allergen (Diaskintest) is a new method for identification that has been implemented in Russia. This study was performed to compare the performances of Diaskintest and QuantiFERON-TB Gold (QFT) in adults and children with suspected TB in Moscow, Russia.Adults (n=85) and children (n=96) were tested using Diaskintest and QFT. Concordance and comparative analyses were performed.Diaskintest and QFT were concordant in 84% of adults and 90% of children (overall concordance 87%, κ>0.6, Kc>0.5). The concordance between QFT, Diaskintest, and the final diagnosis was good in adults (86% and 81%, respectively) and moderate in children (77% and 79%, respectively). In adults, QFT had a higher sensitivity for detecting TB than Diaskintest (82% and 68%, respectively); in children, Diaskintest was more sensitive (73% and 65%, respectively). In patients with a confirmed TB diagnosis, negative Diaskintest/QFT results were associated with low disease activity. Combined Diaskintest/QFT results identified TB patients with higher sensitivity and specificity than each test separately.Diaskintest is a low-cost diagnostic tool that shows a test positivity rate similar to QFT and can be used in combination with QFT as an adjunctive test for TB diagnosis.

Concordance

QuantiFERON

Gold standard (test)

Tuberculosis diagnosis

10.1016/j.ijid.2019.06.014

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Citations (23)