Chunling Zhao

First Affiliated Hospital of Zhengzhou University

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Xiaowen Zheng

Zhejiang University

Jia L. Zhuo

Tulane University

Zhiqin Gao

Weifang Medical University

Wentong Li

Jilin University

Jiao Su

First Affiliated Hospital of Zhengzhou University

Xiao C. Li

Tulane University

Liping Dai

First Affiliated Hospital of Henan University

Songyun Ouyang

First Affiliated Hospital of Zhengzhou University

Songyun Ouyang

First Affiliated Hospital of Zhengzhou University

Jiyu Ju

Weifang Medical University

Cooperative Institutions

Weifang Medical University

104

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Sun Yat-sen University

Zhengzhou University

First Affiliated Hospital of Zhengzhou University

Southwest Medical University

Fudan University

Nanjing Medical University

Huazhong University of Science and Technology

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Wnt3a mitigates acute lung injury by reducing P2X7 receptor-mediated alveolar epithelial type I cell death

Cell Death and Disease (2014)

Yujie Guo Amarjit Mishra Tingting Weng Narendranath Reddy Chintagari Y Wang

Acute lung injury (ALI) is characterized by pulmonary endothelial and epithelial cell damage, and loss of the alveolar–capillary barrier. We have previously shown that P2X7 receptor (P2X7R), a cell death receptor, is specifically expressed in alveolar epithelial type I cells (AEC I). In this study, we hypothesized that P2X7R-mediated purinergic signaling and its interaction with Wnt/β-catenin signaling contributes to AEC I death. We examined the effect of P2X7R agonist 2′-3′-O-(4-benzoylbenzoyl)-ATP (BzATP) and Wnt agonist Wnt3a on AEC I death in vitro and in vivo. We also assessed the therapeutic potential of Wnt3a in a clinically relevant ALI model of intratracheal lipopolysaccharide (LPS) exposure in ventilated mice. We found that the activation of P2X7R by BzATP caused the death of AEC I by suppressing Wnt/β-catenin signaling through stimulating glycogen synthase kinase-3β (GSK-3β) and proteasome. On the other hand, the activation of Wnt/β-catenin signaling by Wnt3a, GSK-3β inhibitor, or proteasome inhibitor blocked the P2X7R-mediated cell death. More importantly, Wnt3a attenuated the AEC I damage caused by intratracheal instillation of BzATP in rats or LPS in ventilated mice. Our results suggest that Wnt3a overrides the effect of P2X7R on the Wnt/β-catenin signaling to prevent the AEC I death and restrict the severity of ALI.

WNT3A

10.1038/cddis.2014.254

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Citations (26)

Role of nicotinic acetylcholine receptors in cognitive function

Chunling Zhao

The nicotinic acetylcholine receptors(nAChR)are the ligand-gated ion-channels and are the the Cys-loop receptors.They are widely expressed in the central and peripheral nervous system.It is well documented that the central nAChR participate in complex functions,such as attention,learning,arousal and cognition.Clinical data suggested their involvement in the pathogenesis of several cognition disorders,for example Alzheimer's disease(AD),Parkinson's disease(PD)and epilepsy.Here,we reviewed some of the latest advances about the sort,structure,distribution of nAChR and the role of central nAChR in cognitive function.

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Targeting TLR2/Rac1/cdc42/JNK Pathway to Reveal That Ruxolitinib Promotes Thrombocytopoiesis

International Journal of Molecular Sciences (2022)

Shuo Yang Xiaoqin Tang Long Wang Chengyang Ni Yuesong Wu

Thrombocytopenia has long been considered an important complication of chemotherapy and radiotherapy, which severely limits the effectiveness of cancer treatment and the overall survival of patients. However, clinical treatment options are extremely limited so far. Ruxolitinib is a potential candidate.The impact of ruxolitinib on the differentiation and maturation of K562 and Meg-01 cells megakaryocytes (MKs) was examined by flow cytometry, Giemsa and Phalloidin staining. A mouse model of radiation-injured thrombocytopenia (RIT) was employed to evaluate the action of ruxolitinib on thrombocytopoiesis. Network pharmacology, molecular docking, drug affinity responsive target stability assay (DARTS), RNA sequencing, protein blotting and immunofluorescence analysis were applied to explore the targets and mechanisms of action of ruxolitinib.Ruxolitinib can stimulate MK differentiation and maturation in a dose-dependent manner and accelerates recovery of MKs and thrombocytopoiesis in RIT mice. Biological targeting analysis showed that ruxolitinib binds directly to Toll Like Receptor 2 (TLR2) to activate Rac1/cdc42/JNK, and this action was shown to be blocked by C29, a specific inhibitor of TLR2.Ruxolitinib was first identified to facilitate MK differentiation and thrombocytopoiesis, which may alleviate RIT. The potential mechanism of ruxolitinib was to promote MK differentiation via activating the Rac1/cdc42/JNK pathway through binding to TLR2.

Ruxolitinib

10.3390/ijms232416137

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Influence of tablet computer games on the treatment compliance and maladaptive behaviors of patients undergoing painless gastroscopy

Zhonghua xiandai huli zazhi (2019)

Chunling Zhao Xiaohuan Li

Objective To observe the improve effect of tablet computer games on treatment compliance and maladaptive behaviors of patients undergoing painless gastroscopy. Methods The 133 patients undergoing painless gastroscopy for the first time in Xinxiang Central Hospital from June 2018 to June 2019 were selected and divided into routine care group (n=65) and tablet computer game group (n=68) by picking method. Routine care group was given routine nursing intervention, while tablet computer game group was given tablet computer games intervention 30 minutes before painless gastroscopy based on routine nursing intervention. The treatment compliance was compared by cooperation questionnaire between two groups, and after painless gastroscopy for 1 week, the incidence of postoperative behavior change were evaluated by postoperative behavior scale (PHBQ) . Results The differences of coordination scores during examination between the two groups were not statistically significant (P>0.05) . The scores of coordination awareness, preparation before examination, posture and other coordination, as well as total scores were higher in tablet computer game group than those in routine care group. The result was statistically significant (P 0.05) . Conclusions The tablet computer game intervention before painless gastroscopy can improve the examination cooperation and reduce the incidence of inappropriate behaviors after examination. It is worthy of clinical promotion. Key words: Gastroscopy; Tablet computer game; Compliance; Maladaptive behaviors

Computer game

10.3760/cma.j.issn.1674-2907.2019.35.011

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Platelet-derived Wnt antagonist Dickkopf-1 is implicated in ICAM-1/VCAM-1–mediated neutrophilic acute lung inflammation

Blood (2015)

Yujie Guo Amarjit Mishra Emily Howland Chunling Zhao Dhananjay Shukla

DKK1

WNT3A

10.1182/blood-2015-02-622233

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Citations (78)

Comparison study of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin microspheres

Wuhan University Journal of Natural Sciences (2009)

LI Ruo-bao Chunling Zhao Weifen Zhang Lihong Shi Jin‐Bao Tang

10.1007/s11859-009-0415-2

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Citations (2)

Distinct fate, dynamics and niches of renal macrophages of bone marrow or embryonic origins

Nature Communications (2020)

Fengming Liu Shen Dai Dechun Feng Zhongnan Qin Xiao Peng

Abstract Renal macrophages (RMs) participate in tissue homeostasis, inflammation and repair. RMs consist of embryo-derived (EMRMs) and bone marrow-derived RMs (BMRMs), but the fate, dynamics, replenishment, functions and metabolic states of these two RM populations remain unclear. Here we investigate and characterize RMs at different ages by conditionally labeling and ablating RMs populations in several transgenic lines. We find that RMs expand and mature in parallel with renal growth after birth, and are mainly derived from fetal liver monocytes before birth, but self-maintain through adulthood with contribution from peripheral monocytes. Moreover, after the RMs niche is emptied, peripheral monocytes rapidly differentiate into BMRMs, with the CX3CR1/CX3CL1 signaling axis being essential for the maintenance and regeneration of both EMRMs and BMRMs. Lastly, we show that EMRMs have a higher capacity for scavenging immune complex, and are more sensitive to immune challenge than BMRMs, with this difference associated with their distinct glycolytic capacities.

Monocyte

Homeostasis

10.1038/s41467-020-16158-z

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Citations (85)

Placental miR-106a∼363 cluster is dysregulated in preeclamptic placenta

Placenta (2014)

C. Zhang Qiu Li Ning Ren C. Li Xiaokai Wang

Pathogenesis

10.1016/j.placenta.2014.11.020

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Citations (36)