Samira Ibala

University of Sousse

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Sonia Brahem

Hôpital Farhat Hached

Mounir Ajina

University of Sousse

Meriem Mehdi

Hospital Fatuma Bourguiba Monastir

Ali Saâd

Hôpital Farhat Hached

Hanène Landolsi

University of Sousse

Moez Gribaa

Hôpital Farhat Hached

Hatem Elghezal

Riyadh Armed Forces Hospital

R. Naifer

Hôpital Farhat Hached

A. Saad

Federal University Dutsin-Ma

M. Mjid

Tunis University

Cooperative Institutions

Medical University of Lublin

Instituto Sapientia

Endokrinologikum

Medecell (Brazil)

Hôpital Farhat Hached

Centre Hospitalier Universitaire de Lille

Centre Hospitalier Universitaire de Tours

Université de Tours

Centre of Biotechnology of Sfax

Physiologie de la Reproduction et des Comportements

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Résumés des POSTERS affichés au XXVème Congrès de la SALF

Andrologie (2008)

N. Bouayed Abdelmoula A. Amouri Mehdi Meddeb A. Ben Hmida Nabih Féki

10.1007/bf03040725

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Hyperstimulation ovarienne induite par un agoniste de la GnRH. À propos d'un cas

Gynécologie Obstétrique & Fertilité (2005)

R. Naifer Mounir Ajina Ghaya Merdassi M. Bibi Samira Ibala

Controlled ovarian hyperstimulation

Human chorionic gonadotropin

Ovarian hyperstimulation syndrome

10.1016/j.gyobfe.2005.06.023

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DNA fragmentation status in patients with necrozoospermia

Systems Biology in Reproductive Medicine (2012)

Sonia Brahem Sonia Jellad Samira Ibala Ali Saâd Meriem Mehdi

The aim of this study was to determine if a relationship exists between the levels of sperm DNA fragmentation and necrospermia in infertile men. Semen samples obtained from 70 men consulting for infertility evaluation were analyzed according to World Health Organization (WHO) guidelines. Patients were subdivided into three groups according to the percentage of necrotic spermatozoa: normozoospermia (<30%; n = 20), moderate necrozoospermia (50-80%; n = 30), and severe necrozoospermia (>80%; n = 20). DNA fragmentation was detected by the terminal desoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick-end labeling (TUNEL) assay. The sperm DNA fragmentation index (DFI) was 9.28 ± 2.98% in patients with a normal level of necrotic spermatozoa, 20.25 ± 3.21% in patients with moderate necrozoospermia, and 35.31 ± 5.25% in patients with severe necrozoospermia. There was a statistically significant increase of DNA fragmentation in the necrozoospermic group (P < 0.01). A strong correlation was found between the degree of necrozoospermia and sperm DNA fragmentation. We concluded that patients with necrozoospermia showed a high level of DNA fragmentation compared to normozoospermic men. Severe necrozoospermia (>80%) is a predictive factor for increased sperm DNA damage.

Fragmentation

Terminal deoxynucleotidyl transferase

10.3109/19396368.2012.710869

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Impact of environmental microplastics alone and mixed with benzo[a]pyrene on cellular and molecular responses of Mytilus galloprovincialis

Journal of Hazardous Materials (2022)

Ilef Romdhani Giuseppe De Marco Tiziana Cappello Samira Ibala Nesrine Zitouni

Comet Assay

10.1016/j.jhazmat.2022.128952

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Citations (56)

Chromosomal segregation in spermatozoa of five Robertsonian translocation carriers t(13;14)

Journal of Assisted Reproduction and Genetics (2011)

Manel Mahjoub Meriem Mehdi Sonia Brahem Hatem Elghezal Samira Ibala

Robertsonian translocation

10.1007/s10815-011-9560-6

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Y chromosome microdeletions in azoospermic and oligozoospermic Tunisian men

Middle East Fertility Society Journal (2017)

G. Saad Ameni Kdissa A. Khelil Mounir Ajina Samir Hidar

In the present work, we aim to determine the frequency of Y microdeletions and to study the clinical and biological characteristics in idiopathic azoospermic and oligozoospermic men originating from the center of Tunisia. A sample of 163 infertile men with normal karyotype was screened for Y chromosome microdeletion. This research was done by two multiplex PCR reactions. The multiplex A where STSs: Sy82 (AZFa), Sy134 (AZFb), Sy254 (AZFc) markers are amplified and the multiplex B where STSs: Sy81 (AZFa), Sy127 (AZFb), Sy255 (AZFc) markers are amplified. All patients had primary spermatogenic failure with normal or elevated plasma FSH level. AZF microdeletion was found in 14 patients (8.58%). The microdeletions affect AZFc in 9 cases, AZFa in one case, AZFb + c in two cases and AZFa + b + c in two cases. An epididymal obstruction is associated in five patients with Y chromosome microdeletion. The incidence of Yq microdeletions in the studied population falls within the range published in other countries. Association between Yq microdeletions and epididymal abnormalities is frequent. Yq microdeletions are inherited when an intracytoplasmic spermatozoa injection (ICSI) is possible which justifies a genetic counseling consultation in order to inform couples about possible risks and to refer them to the most appropriate treatment.

Y chromosome microdeletion

Azoospermia factor

10.1016/j.mefs.2017.11.004

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Citations (1)

Cytogenetic and Molecular Aspects of Absolute Teratozoospermia: Comparison Between Polymorphic and Monomorphic Forms

Urology (2011)

Sonia Brahem Hatem Elghezal Houda Ghédir Hanène Landolsi Abdelbasset Amara

Semen Analysis

10.1016/j.urology.2011.08.064

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Citations (31)

POSTER VIEWING SESSION - EARLY PREGNANCY

Human Reproduction (2011)

Toshiyuki Kamijo Paweł Milart K. Wojcik Piotr Szkodziak Sławomir Woźniak

results in the production of high quality follicles, which in turn, would be responsible for a higher oocyte recovery rate.Furthermore the GnRH agonist treatment is significantly less costly than antagonist treatment.Costs, effectiveness and comfort can be brought together using GnRH agonist treatment in alternate days, allowing the delivery of additional ART cycles in developing countries.

10.1093/humrep/26.s1.78

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Profiles of embryos designed by fertilization assisted and their impact on the rate of pregnancies

Mounir Ajina R. Naifer Imed Harrabi Wafa Loussaïef Samira Ibala

AIM evaluation of our experience in assisted fertilization by ICSI with analysis of prognostic factors. METHODS retrospective study of 199 cycles of ICSI during a 2 years and half period between September 2001 and February 2004. The procedure of ICSI included several stages: collection and preparation of the semen, stake in culture of oocytes, removing of cumulus cells and microinjection of oocytes, control of the fertilization and embryo transfer respectively 18 to 22 hours and 48 hours after the microinjection. RESULTS the mean age of the patients was 32,4 years and the mean duration of infertility was 7 years. The mean fertilization rate was 50%. The mean number of embryos transferred was 2,46. We got 41 pregnancies of which 36 were clinical pregnancies (87,8%). The pregnancy rate was 26,1% by transfer and 21% by retrieval. The women age was the first prognostic factor of ICSI. The pregnancy rate was 27% before the age of 35 years, decreases with age and annul himself after 40 years (P=0,02). The other prognostic factor was the number of 4 cells embryos transferred. The pregnancy rate increases with significant way with the number of 4 cells embryos transferred: 15% after transfer of only one embryo versus 43% after transfer of 3 embryos or more (P=0,04). The ICSI prognostic has not been influenced with significant way by the origin or the sperm mobility, by the duration of infertility and by the total number of embryos transferred. CONCLUSION the ICSI represents currently the treatment of choice of couple having extreme spermatic changes. The results of our study are comparable to those reported in the literature. The women age and the number of 4 cells embryos transferred are the main factors predicting of the ICSI prognostic.

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