Charles Thill

Healthwise

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James L. Coleman

University of Manchester

Melissa A. Prusinski

New York State Department of Health

Joon‐Hak Lee

Korea Fisheries Resources Agency

Sarah Kogut

New York State Department of Health

P. Bryon Backenson

New York State Department of Health

Christopher J. Kuhlow

Stony Brook University

Jorge L. Benach

Stony Brook University

Dreania LeVine

Stony Brook University

Paul Sweazey

Media Working Group

Dennis J. White

New York State Department of Health

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New York State Department of Health

Stony Brook University

State University of New York

Centers for Disease Control and Prevention

New York Medical College

University at Albany, State University of New York

Laboratory of Molecular Genetics

Center for Molecular Medicine and Immunology

Corning (United States)

Wadsworth Center

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Babesia microtiandBorrelia burgdorferiFollow Independent Courses of Infection in Mice

The Journal of Infectious Diseases (2005)

James L. Coleman Dreania LeVine Charles Thill Christopher J. Kuhlow Jorge L. Benach

Reports of coinfection with Borrelia burgdorferi and Babesia microti in tick vectors, reservoir hosts, and patients have led to increased concern that synergism between the 2 organisms may result in illness more severe than that caused by either infection alone. In the present study, we investigated the impact of simultaneous Lyme disease and babesiosis in the mouse model. Young immunocompetent, young asplenic, and aged C3H/HeN mice, as well as young BALB/c mice, were coinfected with B. burgdorferi and B. microti, and disease severity was compared with that in singly infected and uninfected control mice. Babesiosis followed its normal course of infection in coinfected mice, without evidence for increased severity, as reflected by percentage of parasitemia, spleen weights, and hematologic and clinical chemistry parameters. Likewise, Lyme disease followed its established course and severity in coinfected mice, as reflected by the degrees of spirochete dissemination and arthritis. This study demonstrates that, in the mouse model, these 2 infections proceed independently.

Borrelia Burgdorferi Infection

10.1086/496891

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Citations (34)

Babesia microti, Upstate New York

Emerging infectious diseases (2005)

Sarah Kogut Charles Thill Melissa A. Prusinski Joon‐Hak Lee P. Bryon Backenson

Abstract Five cases of human babesiosis were reported in the Lower Hudson Valley Region of New York State in 2001. An investigation to determine if Babesia microti was present in local Ixodes scapularis ticks yielded 5 positive pools in 123 pools tested, the first detection of B. microti from field-collected I. scapularis in upstate New York.

Ixodes scapularis

10.3201/eid1103.040599

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Citations (51)

Digital emulation of consumer-ownable products

2023 IEEE International Conference on Consumer Electronics (ICCE) (2011)

Paul Sweazey Ton Kalker Charles Thill

IEEE P1817 is defining a standard for consumer-ownable instances of digital creative works such as movies, music, books, and games. We avoid usage and sharing restrictions and supplier tethers for consumers. For copyright holders we block counterfeiting and preserve the item singularness.

File Sharing

10.1109/icce.2011.5722625

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Whole-Genome DNA Array Analysis of the Response of Borrelia burgdorferi to a Bactericidal Monoclonal Antibody

Infection and Immunity (2004)

Julie M. Anderton Rafal Tokarz Charles Thill Christopher J. Kuhlow Chad S. Brooks

ABSTRACT Identification and characterization of genes that contribute to infection with Borrelia burgdorferi and, of those, genes that are targets of host responses is important for understanding the pathogenesis of Lyme disease. The complement-independent bactericidal monoclonal antibody (MAb) CB2 recognizes a carboxy-terminal, hydrophilic epitope of the outer surface protein B (OspB). CB2 kills B. burgdorferi by an unknown bactericidal mechanism. Upon binding of CB2 to OspB, differentially expressed gene products may be responsible for, or associated with, the death of the organism. A time course of the response of B. burgdorferi to CB2 was completed to analyze the differential gene expression in the bacteria over a period of visual morphological changes. Bacteria were treated with a sublethal concentration in which spirochetes were visibly distressed by the antibody but not lysed. Preliminary whole-genome DNA arrays at various time points within 1 h of incubation of B. burgdorferi with the antibody showed that most significant changes occurred at 25 min. Circular plasmid 32 (cp32)-encoded genes were active in this period of time, including the blyA homologs, phage holin system genes. DNA array data show that three blyA homologs were upregulated significantly, ≥2 standard deviations from the mean of the log ratios, and a P value of ≤0.01. Quantitative real-time PCR analysis verified blyA and blyB upregulation over an 18- to 35-min time course. The hypothesis to test is whether the killing mechanism of CB2 is through uncontrolled expression of the blyA and blyB phage holin system.

10.1128/iai.72.4.2035-2044.2004

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Citations (20)

Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by Use of Chelex 100 Resin and Polymerase Chain Reaction

Journal of Medical Entomology (2005)

Charles Thill P. Bryon Backenson Melissa A. Prusinski Sarah Kogut Joon‐Hak Lee

A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.

Ixodes scapularis

Parasitiformes

10.1093/jmedent/42.4.694

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Citations (2)

Detection of <I>Babesia microti</I> DNA in <I>Ixodes scapularis</I> (Acari: Ixodidae) by Use of Chelex 100 Resin and Polymerase Chain Reaction

Journal of Medical Entomology (2005)

Charles Thill P. Bryon Backenson Melissa A. Prusinski Sarah Kogut Joon‐Hak Lee

Ixodes scapularis

10.1603/0022-2585(2005)042[0694:dobmdi]2.0.co;2

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Citations (4)