Peng Liu

Hunan Provincial People's Hospital

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Wenjing Sun

Harbin Medical University

Chun Qiao

Nanjing Medical University

Danxia Zhu

Soochow University

Timothy C. Greiner

University of Nebraska Medical Center

C. Miller

Mayo Clinic

Yu‐Jie Wu

East China Normal University

Arthur J. McCullough

Cleveland Clinic

Gauri R. Varadhachary

The University of Texas MD Anderson Cancer Center

S.M. Ismail

University of Malaya

P. Pisters

Case Western Reserve University

Cooperative Institutions

Harbin Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

Sun Yat-sen University

Nanjing Medical University

Chinese Academy of Sciences

Second Affiliated Hospital of Harbin Medical University

Fudan University

Peking University

Shanghai Jiao Tong University

Icahn School of Medicine at Mount Sinai

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Chidamide, a Novel Histone Deacetylase Inhibitor, Inhibits Multiple Myeloma Cells Proliferation through Succinate Dehydrogenase Subunit a

Blood (2018)

Yifeng Sun Peng Liu Jing Li

SDHA

Histone deacetylase inhibitor

Panobinostat

10.1182/blood-2018-99-112747

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Citations (12)

Neuropathology

Laboratory Investigation (2008)

Zein Co Bijan Eghtesad C. Miller Arthur J. McCullough Peng Liu

Neuropathology

10.1038/sj.labinvest.3700738

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Citations (0)

Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases

Molecular Cancer (2023)

Qiang Wang Peng Liu Yingfei Wen Kuan Li Bo Bi

Abstract Clinical hyperthermic intraperitoneal chemotherapy (HIPEC) is regarded as a potential treatment that can prolong survival of patients with peritoneal metastases after cytoreductive surgery. However, treated tumor cells are prone to becoming heat resistant to HIPEC therapy through high expression of heat shock proteins (HSPs). Here, a carrier-free bifunctional nanoinhibitor was developed for HIPEC therapy in the management of peritoneal metastases. Self-assembly of the nanoinhibitor was formed by mixing Mn ion and epigallocatechin gallate (EGCG) in a controllable manner. Such nanoinhibitor directly inhibited HSP90 and impaired the HSP90 chaperone cycle by reduced intracellular ATP level. Additionally, heat and Mn ion synergistically induced oxidative stress and expression of caspase 1, which activated GSDMD by proteolysis and caused pyroptosis in tumor cells, triggering immunogenic inflammatory cell death and induced maturation of dendritic cells through the release of tumor antigens. This strategy to inhibit heat resistance in HIPEC presented an unprecedented paradigm for converting “cold” tumors into “hot” ones, thus significantly eradicating disseminated tumors located deep in the abdominal cavity and stimulating immune response in peritoneal metastases of a mouse model. Collectively, the nanoinhibitor effectively induced pyroptosis of colon tumor cells under heat conditions by inhibiting heat stress resistance and increasing oxidative stress, which may provide a new strategy for treatment of colorectal peritoneal metastases.

Hyperthermic Intraperitoneal Chemotherapy

Peritoneal cavity

Immunogenic cell death

10.1186/s12943-023-01790-2

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Citations (18)

Identification of a novel selective agonist of PPARγ with no promotion of adipogenesis and less inhibition of osteoblastogenesis

Scientific Reports (2015)

Chang Liu Tingting Feng Ningyu Zhu Peng Liu Xiaowan Han

Abstract Nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) plays an important role in the regulation of glucose homeostasis and lipid metabolism. However, current PPARγ-targeting drugs such as thiazolidinediones (TZDs) are associated with undesirable side effects. We identified a small molecular compound, F12016, as a selective PPARγ agonist by virtual screening, which showed moderate PPARγ agonistic activity and binding ability for PPARγ. F12016 did not activate other PPAR subtypes at 30 μM and selectively modulated PPARγ target gene expression. In diabetic KKAy mice, F12016 had insulin-sensitizing and glucose-lowering properties and suppressed weight gain. In vitro, F12016 effectively increased glucose uptake and blocked cyclin-dependent kinase 5-mediated phosphorylation of PPARγ at Ser273, but slightly triggered adipogenesis and less inhibited osteoblastogenesis than rosiglitazone. Moreover, compared with the full agonist rosiglitazone, F12016 had a distinct group of coregulators and a different predicted binding mode for the PPARγ ligand-binding domain. A site mutation assay confirmed the key epitopes, especially Tyr473 in AF-2. In summary, our study shows that F12016 is a non-TZD, novel selective PPARγ agonist without the classical lipogenic side effects, which may provide a new structural strategy for designing PPARγ ligands with advantages over TZDs.

Identification

Promotion (chess)

PPAR agonist

10.1038/srep09530

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Citations (63)

Mechanistic Characterization of De Novo Generation of Variable Number Tandem Repeats in Circular Plasmids during Site‐Directed Mutagenesis and Optimization for Coding Gene Application

Advanced Biology (2024)

Ziqi Hu Guochao Lin Mingzhu Zhang Shengwen Piao Jiankun Fan

Site-directed mutagenesis for creating point mutations, sometimes, gives rise to plasmids carrying variable number tandem repeats (VNTRs) locally, which are arbitrarily regarded as polymerase chain reaction (PCR) related artifacts. Here, the alternative end-joining mechanism is reported rather than PCR artifacts accounts largely for that VNTRs formation and expansion. During generating a point mutation on GPLD1 gene, an unexpected formation of VNTRs employing the 31 bp mutagenesis primers is observed as the repeat unit in the pcDNA3.1-GPLD1 plasmid. The 31 bp VNTRs are formed in 24.75% of the resulting clones with copy number varied from 2 to 13. All repeat units are aligned with the same orientation as GPLD1 gene. 43.54% of the repeat junctions harbor nucleotide mutations while the rest don't. Their demonstrated short primers spanning the 3' part of the mutagenesis primers are essential for initial creation of the 2-copy tandem repeats (TRs) in circular plasmids. The dimerization of mutagenesis primers by the alternative end-joining in a correct orientation is required for further expansion of the 2-copy TRs. Lastly, a half-double priming strategy is established, verified the findings and offered a simple method for VNTRs creation on coding genes in circular plasmids without junction mutations.

Direct repeat

Coding region

10.1002/adbi.202400084

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Expression of angiogenic factors and their clinical significances in acute myeloid leukemia

Chinese Journal of Cancer (2004)

Yi Wang Zhijian Xiao Peng Liu Zhi Peng Zhongchao Han

Source

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Citations (5)

Influence of dibutyl phthalate on the gene expression of P450scc of ovary in pubertal female rats

Peng Liu

Objective: To study the influences of dibutyl phthalate(DBP) on the estrous cycle and endocrine of puberty female rats.Methods: 21-day-old female SD rats were randomly divided into 250 mg/kg,500 mg/kg and 1 000 mg/kg experimental groups and one control group(corn oil),the rats were administrated with DBP by gavage for 8 weeks.Female rats were sacrificed,during the proestrus,and the blood was collected,and the serum levels of progesterone(P) were examined by radioimmunoassay.The mRNA levels of cholesterol side-chain cleavage enzyme(P450scc) of ovaries were detected by real-time fluorescent quantitative polymerase chain reaction(RTPCR).Results: There were statistically significant differences of serum progesterone levels among treatment and control groups(F=4.306,P=0.014),the serum progesterone level in 1 000 mg/kg does group was lower than that in the control group(P0.05),while there was no significant difference of serum progesterone between 250 mg/kg or 500 mg/kg groups and the control group(P0.05).The expression levels of P450scc mRNA in ovarian were significant different in treatment and the control groups(H=15.591,P0.01),compared with the control group,the expression levels of P450scc mRNA in three treatment groups were lower(P0.01),but there were not significant differences in expression levels of P450scc mRNA among three treatment groups(P0.05).Conclusion: DBP exposure have reproductive toxicity to female rats.

Dibutyl phthalate

Corn oil

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ATP‐binding cassette B1 gene polymorphisms, mRNA expression and chemosensitivity to paclitaxel in non‐small cell lung cancer cells

Respirology (2011)

Xiangning Meng Geng Wang Peng Liu Jingmei Hou Yan Jin

The adenosine triphosphate (ATP)-binding cassette, sub-family B, member 1 (ABCB1) gene encodes P-glycoprotein (Pgp), which plays an important role in drug disposition by limiting intracellular uptake of paclitaxel. ABCB1 gene polymorphisms may alter the expression and function of Pgp, thereby influencing the response to chemotherapy. A panel of 17 non-small cell lung cancer (NSCLC) cell lines was used to investigate whether alterations in the ABCB1 gene or its mRNA expression correlated with in vitro chemosensitivity to paclitaxel.Polymorphisms in the ABCB1 gene were evaluated by direct sequencing. mRNA expression levels were assessed by quantitative real-time reverse transcription PCR. In vitro chemosensitivity to paclitaxel was expressed as half-maximal inhibitory concentration values, using a tetrazolium (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)-based colorimetric assay.The variant allele frequencies for four ABCB1 gene polymorphisms were 14.71% for 2677G>T/A, 32.35% for 2734T>C, 23.53% for 3396C>T and 76.47% for 3435C>T. There was a significant positive correlation between ABCB1 mRNA expression and half-maximal inhibitory concentration values for paclitaxel (r=0.5322, P=0.0279). None of the four ABCB1 gene polymorphisms were associated with paclitaxel chemosensitivity or ABCB1 mRNA expression in the 17 cell lines.These in vitro results suggest that high ABCB1 mRNA expression may be a predictive biomarker for poor chemosensitivity to paclitaxel. The panel of NSCLC cell lines may provide clues and indications for establishing clinically useful relationships between a given polymorphism or level of gene expression and chemosensitivity to an anti-cancer agent.

Chemosensitivity assay

10.1111/j.1440-1843.2011.02050.x

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Citations (7)

Neuropathology

Modern Pathology (2008)

Zein Co Bijan Eghtesad C. Miller Arthur J. McCullough Peng Liu

Neuropathology

10.1038/sj.modpathol.3801042

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Expression and clinical value of micro RNA-590 in oral mucosa of patients with oral lichen planus and oral squamous cell carcinoma