Diane R. Stothard

Eli Lilly (United States)

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Robert B. Jones

Indiana University – Purdue University Indianapolis

Paul A. Fuerst

The Ohio State University

Barbara Van Der Pol

University of Alabama at Birmingham

Axel R. Fugl‐Meyer

Umeå University

Stanley E. Althof

University School

Irène Buvat

Inserm

Mark Belger

Eli Lilly (United Kingdom)

Thomas J. Byers

University of Bath

James A. Williams

GlaxoSmithKline (United States)

Mohammad Awwad

The Ohio State University

Cooperative Institutions

Indiana University – Purdue University Indianapolis

Indiana University School of Medicine

The Ohio State University

Indiana University

Eli Lilly (United States)

The University of Texas Medical Branch at Galveston

Uppsala University

Richard L. Roudebush VA Medical Center

University School

Umeå University

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Aspects of Sexual Satisfaction in Men with Erectile Dysfunction: A Factor Analytic and Logistic Regression Approach

The Journal of Sexual Medicine (2009)

Axel R. Fugl‐Meyer Stanley E. Althof Irène Buvat M Paget Mariano Sotomayor

10.1111/j.1743-6109.2008.01135.x

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Citations (9)

Infections with atypical mycobacteria in British Columbia.

PubMed (1969)

Stefan Grzybowski M. T. Brown Diane R. Stothard

Mycobacterium Infections

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Citations (8)

RepeatedChlamydia trachomatisGenital Infections in Adolescent Women

The Journal of Infectious Diseases (2009)

Byron E. Batteiger Wanzhu Tu Susan Ofner Barbara Van Der Pol Diane R. Stothard

Repeated Chlamydia trachomatis infections are common among young sexually active women. The relative frequency of reinfection and antibiotic treatment failure is undefined.Adolescent women enrolled in a longitudinal cohort had behavioral and sexually transmitted infection assessments performed every 3 months, including amplification tests for C. trachomatis, ompA genotyping, and interviews and diary entries to document sex partner-specific coitus and event-specific condom use. Repeated infections were classified as reinfection or treatment failure by use of an algorithm. All infections for which treatment outcomes were known were used to estimate the effectiveness of antibiotic use.We observed 478 episodes of infection among 210 study participants; 176 women remained uninfected. The incidence rate was 34 episodes/100 woman-years. Of the women who were infected, 121 experienced 1 repeated infections, forming 268 episode pairs; 183 pairs had complete data available and were classified using the algorithm. Of the repeated infections, 84.2% were definite, probable, or possible reinfections; 13.7% were probable or possible treatment failures; and 2.2% persisted without documented treatment. For 318 evaluable infections, we estimated 92.2% effectiveness of antibiotic use.Most repeated chlamydial infections in this high-incidence cohort were reinfections, but repeated infections resulting from treatment failures occurred as well. Our results have implications for male screening and partner notification programs and suggest the need for improved antibiotic therapies.

Sex organ

10.1086/648734

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Citations (262)

The evolutionary history of the genus Rickettsia as inferred from 16S and 23S ribosomal RNA Genes and the 17 kilodalton cell surface antigen gene

Diane R. Stothard

23S ribosomal RNA

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Use of a Reverse Dot Blot Procedure To Identify the Presence of Multiple Serovars inChlamydia trachomatisUrogenital Infection

Journal of Clinical Microbiology (2001)

Diane R. Stothard

Epidemiologic research requires identification of Chlamydia trachomatis serovars and detection of mixed infection. Antibody-based serotyping is unworkable when specimens are urine or vaginal swabs. We developed a reverse dot blot (RDB) to screen for multiple serotypes in these specimens. RDB yielded the predicted results on all artificially mixed samples and on seven of eight clinically mixed samples.

Dot blot

Chlamydia trachomatis infection

10.1128/jcm.39.7.2655-2659.2001

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Citations (22)

Identification of a Chlamydia trachomatis Serovar E Urogenital Isolate Which Lacks the Cryptic Plasmid

Infection and Immunity (1999)

Diane R. Stothard James A. Williams Barbara Van Der Pol Robert B. Jones

Identification

10.1128/.67.4.2051-2051.1999

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Citations (7)

Ancestral Divergence of Rickettsia bellii from the Spotted Fever and Typhus Groups of Rickettsia and Antiquity of the Genus Rickettsia

International Journal of Systematic Bacteriology (1994)

Diane R. Stothard J. B. CLARK Paul A. Fuerst

The eubacterial genus Rickettsia belongs to the alpha subgroup of the phylum Proteobacteria. This genus is usually divided into three biotypes on the basis of vector host and antigenic cross-reactivity characteristics. However, the species Rickettsia bellii does not fit into this classification scheme; this organism has characteristics common to both the spotted fever group and the typhus group biotypes and also exhibits some unique features. Sequences of the 16S rRNA and 23S rRNA genes from Rickettsia rickettsii (spotted fever group), Rickettsia prowazekii (typhus group), and R. bellii were studied to determine the position of R. bellii in the rickettsial classification scheme. The 23S rRNA gene sequences described in this paper are the first 23S rRNA sequences reported for any member of the Rickettsiaceae. The 23S rRNA gene contains substantially more phylogenetic information than is contained in the 16S rRNA sequences, and the 23S rRNA gene sequence has diverged about 1.9 times faster in the three Rickettsia species which we studied. Taken together, the molecular data obtained from the two genes indicate that R. bellii is not a member of either the spotted fever group or the typhus group; rather, this organism appears to be the product of a divergence which predates the separation of the genus into the spotted fever group and the typhus group. Consequently, different combinations of the ancestral characteristics retained by R. bellii have been retained in the more derived lineages of the genus.(ABSTRACT TRUNCATED AT 250 WORDS)

23S ribosomal RNA

Rickettsia prowazekii

10.1099/00207713-44-4-798

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Citations (105)

Is there a role for Chlamydia pneumoniae in hemodialysis vascular access thrombosis?

American Journal of Kidney Diseases (2000)

Anthony W. Zydlewski Diane R. Stothard Barbara Van Der Pol Robert B. Jones Ronald S. Filo

10.1053/ajkd.2000.19813

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Citations (2)

The Evolutionary History of the Genus Acanthamoeba and the Identification of Eight New 18S rRNA Gene Sequence Types

Journal of Eukaryotic Microbiology (1998)

Diane R. Stothard Jill M. Schroeder‐Diedrich Mohammad Awwad Rebecca J. Gast Dolena R. Ledee

ABSTRACT The 18S rRNA gene ( Rns ) phylogeny of Acanthamoeba is being investigated as a basis for improvements in the nomenclature and taxonomy of the genus. We previously analyzed Rns sequences from 18 isolates from morphological groups 2 and 3 and found that they fell into four distinct evolutionary lineages we called sequence types T1‐T4. Here, we analyzed sequences from 53 isolates representing 16 species and including 35 new strains. Eight additional lineages (sequence types T5‐T12) were identified. Four of the 12 sequence types included strains from more than one nominal species. Thus, sequence types could be equated with species in some cases or with complexes of closely related species in others. The largest complex, sequence type T4, which contained six closely related nominal species, included 24 of 25 keratitis isolates. Rns sequence variation was insufficient for full phylogenetic resolution of branching orders within this complex, but the mixing of species observed at terminal nodes confirmed that traditional classification of isolates has been inconsistent. One solution to this problem would be to equate sequence types and single species. Alternatively, additional molecular information will be required to reliably differentiate species within the complexes. Three sequence types of morphological group 1 species represented the earliest divergence in the history of the genus and, based on their genetic distinctiveness, are candidates for reclassification as one or more novel genera.

Sequence (biology)

10.1111/j.1550-7408.1998.tb05068.x

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Citations (402)

Rickettsia felis: molecular characterization of a new member of the spotted fever group.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY (2001)

Donald H. Bouyer John Stenos Patricia A. Crocquet-Valdes Cecilia Morón Vsevolod L. Popov

In this report, placement of Rickettsia felis in the spotted fever group (SFG) rather than the typhus group (TG) of Rickettsia is proposed. The organism, which was first observed in cat fleas (Ctenocephalides felis) by electron microscopy, has not yet been reported to have been cultivated reproducibly, thereby limiting the standard rickettsial typing by serological means. To overcome this challenge, several genes were selected as targets to be utilized for the classification of R. felis. DNA from cat fleas naturally infected with R. felis was amplified by PCR utilizing primer sets specific for the 190 kDa surface antigen (rOmpA) and 17 kDa antigen genes. The entire 5,513 bp rompA gene was sequenced, characterized and found to have several unique features when compared to the rompA genes of other Rickettsia. Phylogenetic analysis of the partial sequence of the 17 kDa antigen gene indicated that R. felis is less divergent from the SFG rickettsiae than from the TG rickettsiae. The data corroborate results from previous reports that analysed the citrate synthase, 16S rRNA, rompB (135 kDa surface antigen), metK, ftsY, polA and dnaE genes that placed R. felis as a member of the SFG. The organism is passed trans-stadially and transovarially, and infection in the cat flea has been observed in the midgut, tracheal matrix, muscle, hypodermis, ovaries and testes.

Ctenocephalides

10.1099/00207713-51-2-339

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Citations (140)