P. K. Chakrabarty

Presidency University

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R. L. Chavhan

Marathwada Agricultural University

Arun Chatterjee

Thapar Institute of Engineering & Technology

D. Monga

Central Institute for Cotton Research

Walter Gassmann

University of Missouri

B. M. Khadi

University of Agricultural Sciences, Dharwad

Bipinchandra B. Kalbande

Central Institute for Cotton Research

V. R. Hinge

Marathwada Agricultural University

Yagya Dev Mishra

Atal Bihari Vajpayee Indian Institute of Information Technology and Management

A. Bhattacharya

National Institute of Mental Health and Neurosciences

Ulhas Sopanrao Kadam

Gyeongsang National University

Cooperative Institutions

Central Institute for Cotton Research

Indian Council of Agricultural Research

Indian Agricultural Research Institute

Sylhet Agricultural University

Marathwada Agricultural University

Plant (United States)

University of Missouri

University of Delhi

Fundo de Defesa da Citricultura

University of Florida

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Use of an rRNA internal transcribed spacer region to distinguish closely related isolates of the genera Rhizoctonia

Indian Journal of Genetics and Plant Breeding (The) (2008)

D. Monga Mukesh Kumar P. K. Chakrabarty

Inter-and intra specific variation among isolates of Rhizoctonia bataticola and R. solani the causal organism of root rot of cotton were evaluated by analysis of the internal transcribed spacer (ITS) sequences of the rRNA region. The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and amplification products, which ranged between 540 to 680 bp were obtained for all the isolates analyzed.The degree of polymorphism observed did not allow proper identification of most of the isolates. Analysis of two internal transcribed spacer sequences ITS 1 and ITS 2, revealed that within the isolates of R. bataticola, the size of ITS 1 region ranged from 135 to 184 bp while the size of ITS 2 varied by 4 bp. The size of ITS 1 ranged from 155 to 205 bp, while the size of ITS 2 ranged from 149 to 251 bp in isolates of R. solani. Based on phylogenetic analysis of the ITS 1 and ITS2 sequences different isolates of R. bataticola and R. solani were grouped in separate clusters.

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Bacterial Products Important for Plant Disease: Extracellular Enzymes

CRC Press eBooks (2004)

P. K. Chakrabarty Arun Chatterjee Walter Gassmann Arun Chatterjee

10.1081/e-epcs-120021340

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Plasmid-borne determinants of pigmentation, exopolysaccharide production, and virulence in Xanthomonas campestris pv. malvacearum

Canadian Journal of Microbiology (1995)

P. K. Chakrabarty Sheo Raj M. K. Meshram A. Mahadevan Dean W. Gabriel

Three plasmids of 55.0, 31.2, and 7.4 kb, designated as pXCM18-I, pXCM18-II, and pXCM18-III, respectively, were identified and isolated from strain HR13B/2 of Xanthomonas campestris pv. malvacearum. The bacterium was cured of all three plasmids in high frequency (3.1%) with heat (42 °C). The cured strains were nonmucoid and light yellow and were greatly impaired in exopolysaccharide production and virulence. Transformation of a cured strain using a mixture of purified plasmid DNA from HR13B/2 resulted in colonies carrying all six possible combinations of the three plasmids. Of the transformants, two morphologically distinct colony types were observed. The first type of transformants were mucoid and deep yellow, not distinguished from wild-type colonies in morphology, and fully complemented for exopolysaccharide production. These transformants invariably carried pXCM18-II. The second type of transformants were not distinguished from the untransformed strain in morphology but were found to be transformed after screening for plasmid content. These transformants carried either pXCM18-I, pXCM18-III, or both, but never pXCM18-II. In pathogenicity tests, each of the three plasmids restored virulence significantly, and additively, with plasmid pXCM18-I playing a predominant role.Key words: Xanthomonas campestris pv. malvacearum, cotton, plasmid, pigmentation, exopolysaccharide, virulence.

Strain (injury)

Xanthomonas

10.1139/m95-101

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Citations (7)

Characterization of Variability in Some Pathogenic Species of Alternaria Based on the Nucleotide Sequence of Ribosomal DNA

Journal of Plant Biochemistry and Biotechnology (2008)

B. P. Kadam R. L. Chavhan P. K. Chakrabarty F. S. Patil

Spacer DNA

Primer (cosmetics)

Alternaria brassicae

10.1007/bf03263296

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Citations (3)

Rapid and Efficient Protocols for Throughput Extraction of High Quality Plasmid DNA from Strains of Xanthomonas axonopodis pv malvacearum and Escherichia coli

Journal of Plant Biochemistry and Biotechnology (2009)

P. K. Chakrabarty R. L. Chavhan Amit K. Ghosh Dean W. Gabriel

Plasmid preparation

Alkaline lysis

Xanthomonas

10.1007/bf03323444

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Citations (8)

Association of new fungal species with leaf spot and blight of sunflower and cloning of their ribosomal RNA genes.

Indian Phytopathology (2008)

R. L. Chavhan P. K. Chakrabarty F. S. Patil H. B. Patil Surjeet Singh

Phoma

Bipolaris

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Cloning and Characterization of a Member of theXanthomonas avr/pthGene Family That Evades All Commercially Utilized CottonRGenes in the United States

Phytopathology (1997)

P. K. Chakrabarty Y. P. Duan Dean W. Gabriel

ABSTRACT The highly virulent African strains of Xanthomonas campestris pv. malvacearum are quarantined pathogens in the United States and can evade or overcome all commercially utilized resistance (R) genes in cotton grown in the United States including the entire set of host differential lines used to distinguish 19 races of the pathogen. Nevertheless, the African strains carry multiple DNA fragments that strongly hybridize with members of the Xanthomonas avirulence (avr)/pathogenicity (pth) gene family. Since all previously tested members of the gene family confer avirulence against one or more R genes in cotton, strains carrying multiple members might not be expected to evade so many different R genes. The hybridizing DNA fragments were cloned from African strain XcmN and found to confer water-soaking ability to a nearly asymptomatic mutant strain of the pathogen. Restriction mapping, Southern hybridization, and DNA sequencing of the cloned fragments from XcmN were used to identify two water-soaking genes, pthN and pthN2, as new members of the Xanthomonas avr/pth gene family. The complete DNA sequence of pthN was obtained, and it is >94% identical with all other sequenced members of the gene family. Gene fusions of pthN with avrb6 (another family member) and other experiments revealed that the ability of African strain XcmN to water-soak cotton and avoid recognition by commercially used cotton R genes is determined by the specific repeats of multiple functional members of the Xanthomonas avr/pth gene family.

Xanthomonas

R gene

Cloning (programming)

10.1094/phyto.1997.87.11.1160

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Citations (31)

Multiplex molecular marker-assisted analysis of significant pathogens of cotton (Gossypium sp.)

Biocatalysis and Agricultural Biotechnology (2022)

R. L. Chavhan S. Sable A.V. Narwade V. R. Hinge Bipinchandra B. Kalbande

Alternaria solani

Primer (cosmetics)

Gossypium