Haigang Ma

Yangzhou University

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i-10 index

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Yujiao Gao

Yangzhou University

Yi Dai

Children's Hospital of Chongqing Medical University

Jinghua Xiao

Huazhong Agricultural University

Shiping Wang

Shanghai Jiao Tong University

Juntao Shi

Yangzhou University

Xianghua Li

Huazhong Agricultural University

Hongxiang Ma

Yangzhou University

Hongxiang Ma

Lanzhou University Second Hospital

Qinglu Zhang

Institute of Plant Protection

Jianmin Chen

Fudan University

Cooperative Institutions

Huazhong Agricultural University

Yangzhou University

Chinese Academy of Agricultural Sciences

Chinese Academy of Sciences

Central South University

University of Chinese Academy of Sciences

Nanjing Medical University

Henan Agricultural University

Chinese Academy of Medical Sciences & Peking Union Medical College

Capital Medical University

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Transcriptome-based analysis of mitogen-activated protein kinase cascades in the rice response to Xanthomonas oryzae infection

Rice (2015)

Zeyu Yang Haigang Ma Hanming Hong Wen Yao Weibo Xie

Mitogen-activated protein (MAP) kinase cascades, with each cascade consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK), play important roles in dicot plant responses to pathogen infection. However, no single MAP kinase cascade has been identified in rice, and the functions of MAP kinase cascades in rice - pathogen interactions are unknown.To explore the contribution of MAP kinase cascade in rice in response to Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial blight, one of the devastating diseases of rice worldwide, we performed a comprehensive expression analysis of rice MAP kinase cascade genes. We transcriptionally analyzed all the 74 MAPKKK genes, 8 MAPKK, and 17 MAPK genes in two pairs of susceptible and resistant rice lines, with each pair having the same genetic background, to determine the rice response to Xoo infection. The expression of a large number of MAP kinase cascade genes changed in response to infection, and some of the genes also showed different expression in resistant and susceptible reactions. In addition, some MAPKKK genes co-expressed with MAPKK and/or MAPK genes, and MAPKK genes co-expressed with MAPK genes.These results provide a new perspective regarding the putative roles of rice MAP kinase gene candicates and potential cascade targets for further characterization in rice-pathogen interactions.

MAPK cascade

MAP2K7

c-Raf

Cyclin-dependent kinase 9

10.1186/s12284-014-0038-x

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Citations (39)

Two VQ Proteins are Substrates of the OsMPKK6-OsMPK4 Cascade in Rice Defense Against Bacterial Blight

Rice (2021)

Na Li Zeyu Yang Juan Li Wenya Xie F. Xiao‐Feng Qin

Abstract Background The plant-specific valine-glutamine (VQ) protein family with the conserved motif FxxxVQxLTG reportedly functions with the mitogen-activated protein kinase (MAPK) in plant immunity. However, the roles of VQ proteins in MAPK-mediated resistance to disease in rice remain largely unknown. Results In this study, two rice VQ proteins OsVQ14 and OsVQ32 were newly identified to function as the signaling components of a MAPK cascade, OsMPKK6-OsMPK4, to regulate rice resistance to Xanthomonas oryzae pv. oryzae ( Xoo ). Both OsVQ14 and OsVQ32 positively regulated rice resistance to Xoo . In vitro and in vivo studies revealed that OsVQ14 and OsVQ32 physically interacted with and were phosphorylated by OsMPK4. OsMPK4 was highly phosphorylated in transgenic plants overexpressing OsMPKK6 , which showed enhanced resistance to Xoo . Meanwhile, phosphorylated OsVQ14 and OsVQ32 were also markedly accumulated in OsMPKK6- overexpressing transgenic plants. Conclusions We discovered that OsVQ14 and OsVQ32 functioned as substrates of the OsMPKK6-OsMPK4 cascade to enhance rice resistance to Xoo , thereby defining a more complete signal transduction pathway for induced defenses.

MAPK cascade

Genetically modified rice

10.1186/s12284-021-00483-y

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Citations (25)

Molecular Characterization and Marker Development of the HMW-GS Gene from Thinopyrum elongatum for Improving Wheat Quality

International Journal of Molecular Sciences (2023)

Yi Dai Jinfeng Li Juntao Shi Yujiao Gao Haigang Ma

The quality of wheat primarily depends on its storage protein quality, especially in regards to gluten content and high-molecular-weight glutenin subunits (HMW-GS). The number of HMW-GS alleles is limited in bread wheat (Triticum aestivum L.), whereas it is abundant in wheat relatives. Therefore, HMW-GS alleles from wheat relatives could provide a potential for improving quality in wheat breeding. Thinopyrum elongatum (EE) is one of the relatives of wheat. The E genome is closely related to the ABD genome in wheat; therefore, Th. elongatum is often used as an excellent exogenous gene donor for wheat genetic improvement. In this study, the high-molecular glutenin subunit gene was cloned and sequenced from Th. elongatum. A specific molecular marker for identifying the Glu-1Ey subunit gene was developed and applied to detected wheat-Th. elongatum alien introgression lines. Quality analysis indicated that the substitution and addition lines containing Th. elongatum alleles significantly (p < 0.05) increased grain protein content by 3.76% to 5.11%, wet-gluten content by 6.55% to 8.73%, flour 8-MW by 0.25% to 6.35%, and bread volume value by 33.77 mL to 246.50 mL, in comparing it with Chinese Spring. The GMP content and lactic acid SRC showed significant positive correlations with flour processing quality and might be used as indicators for wheat quality. The results were expected to provide a novel route for improving processing quality in wheat quality breeding.

Glutenin

Introgression

Common wheat

Molecular marker

10.3390/ijms241311072

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Citations (3)

The regulation of grain weight in wheat

Seed Biology (2023)

Yujiao Gao Yongsheng Li Weiyi Xia Mengqing Dai Yi Dai

Wheat (Triticum aestivum L., AABBDD) is one of the world's most extensively cultivated crops, furnishing vital nutrients and energy for human consumption. Wheat seeds are the primary sustenance source. Given the mounting global population and dwindling arable land, enhancing wheat grain yield remains a concern for mankind. A pivotal agronomic trait influencing grain yield is grain weight, which is predominantly contingent on seed size and endosperm components and is regulated by complex and precise molecular networks. Endogenous factors, such as transcriptional and post-translational regulators, exert pivotal influence over seed development. Notably, starch is the main storage component of wheat endosperm, and the starch synthesis-related genes exert an important effect on grain weight. Prior reviews on wheat grain traits have mostly focused on the regulation of grain size, and the contents of such reviews are almost entirely written based on the regulatory network of rice seed size. Although many regulatory mechanisms for various traits are similar in rice and wheat, there are lots of differences in wheat due to its vast and intricate genome. An all-encompassing panorama of the grain weight regulatory network has not yet been comprehensive. This review summarizes the catalog of reported genes, discusses the emerging molecular mechanisms, and delves into regulatory networks to foster a more holistic understanding of the intricate regulation of wheat seed weight.

Arable land

Staple food

10.48130/seedbio-2023-0017

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Citations (3)

Regulatory Mechanisms of Mitogen-Activated Protein Kinase Cascades in Plants: More than Sequential Phosphorylation

International Journal of Molecular Sciences (2022)

Haigang Ma Yujiao Gao Yonggang Wang Yi Dai Hongxiang Ma

Mitogen-activated protein kinase (MAPK) cascades play crucial roles in almost all biological processes in plants. They transduce extracellular cues into cells, typically through linear and sequential phosphorylation and activation of members of the signaling cascades. However, accumulating data suggest various regulatory mechanisms of plant MAPK cascades in addition to the traditional phosphorylation pathway, in concert with their large numbers and coordinated roles in plant responses to complex ectocytic signals. Here, we highlight recent studies that describe the uncanonical mechanism of regulation of MAPK cascades, regarding the activation of each tier of the signaling cascades. More particularly, we discuss the unusual role for MAPK kinase kinases (MAPKKKs) in the regulation of MAPK cascades, as accumulating data suggest the non-MAPKKK function of many MAPKKKs. In addition, future work on the biochemical activation of MAPK members that needs attention will be discussed.

Phosphorylation cascade

MAPK cascade

10.3390/ijms23073572

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Citations (16)

Transfer of the Resistance to Multiple Diseases from a Triticum-Secale-Thinopyrum Trigeneric Hybrid to Ningmai 13 and Yangmai 23 Wheat Using Specific Molecular Markers and GISH

Genes (2022)

Yi Dai Juntao Shi Li JinFeng Yujiao Gao Haigang Ma

The middle to lower reaches of the Yangtze River are China’s second largest area for wheat production; wheat disease is more serious there than in other areas because of the high humidity and warm weather. However, most cultivated varieties are susceptible to Fusarium head blight (FHB), powdery mildew, and stripe rust, and the lack of disease-resistant germplasm is an obstacle in wheat breeding. Rye and Thinopyrum elongatum, related species of wheat, carry many genes involved in disease resistance. In this study, a trigeneric hybrid, YZU21, with resistance to FHB, powdery mildew, and stripe rust was used to improve two major wheat cultivars, Ningmai 13 (NM13) and Yangmai 23 (YM23). Specific molecular markers and GISH were used to identify hybrid progenies. Five addition or substitution lines and one translocation line of the Triticum-Secale-Thinopyrum trigeneric hybrid were obtained and evaluated for agronomic traits and the resistance to multiple diseases. The results showed that the six trigeneric hybrid lines had desirable agronomic traits and improved resistance to FHB, powdery mildew, and stripe rust; they might be used as parents in wheat breeding for the resistance to multiple disease.

Germ plasm

Common wheat

Secale

Wheat leaf rust

Rust (programming language)

10.3390/genes13122345

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Citations (4)

OsVQ1 links rice immunity and flowering via interaction with a mitogen-activated protein kinase OsMPK6

Plant Cell Reports (2021)

Peilun Wang Juan Li Zhenzhen Zhang Qinglu Zhang Xianghua Li

MAPK cascade

10.1007/s00299-021-02766-6

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Citations (8)

Transcriptome analysis revealed the potential mechanism of a wheat-Th. elongatum translocation line YNM158 against Fusarium head blight

Research Square (Research Square) (2024)

Yi Dai Wenlin Fei Shiqiang Chen Juntao Shi Haigang Ma

Abstract Background Fusarium head blight (FHB) caused by Fusarium graminearum species complex is a destructive disease in wheat worldwide. Lack of FHB resistant germplasm is a barrier in wheat breeding for the resistance to FHB. Thinopyrum elongatum is an important relative species successfully used for genetic improvement in wheat. Results In this study, a translocation line YNM158 with a YM158 genetic background and carrying the fragment of diploid Th. elongatum 7EL chromosome created by ⁶⁰Co-γ radiation showed high resistance to FHB under both filed and greenhouse conditions. The transcriptome analysis validated that the horizontal transfer gene GST is one of the important contributors to FHB resistance in pathogen infection stage, whereas 7EL chromosome fragment also carries other genes regulated by F. graminearum during the colonization stage. In addition, the introgression of 7EL fragment affected the expression of wheat genes which were enriched in the resistance pathways including phosphatidylinositol signaling system, protein processing in endoplasmic reticulum, plant-pathogen interaction and MAPK signaling pathway at different stages after F. graminearium infection. Conclusions The study provides a novel germplasm for wheat resistance to FHB and new insights into the molecular mechanism of wheat resistance to FHB.

Line (geometry)

10.21203/rs.3.rs-4079736/v1

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Citations (0)

MAPK kinase 10.2 promotes disease resistance and drought tolerance by activating different MAPKs in rice

The Plant Journal (2017)

Haigang Ma Jie Chen Zhenzhen Zhang Ling Ma Zeyu Yang

Mitogen-activated protein kinase (MAPK) cascades, with each cascade consisting of a MAPK kinase kinase (MAPKKK), a MAPK kinase (MAPKK) and a MAPK, have important roles in different biological processes. However, the signal transduction in rice MAPK cascades remains to be elucidated. We show that the structural non-canonical MAPKK, MPKK10.2, enhances rice resistance to Xanthomonas oryzae pv. oryzicola (Xoc), which causes bacterial streak disease, and increases rice tolerance to drought stress by phosphorylating and activating two MAPKs, MPK6 and MPK3, respectively. MPKK10.2-overexpressing (oe) plants showed enhanced resistance to both Xoc and drought, whereas MPKK10.2-RNA interference (RNAi) plants had increased sensitivity to both Xoc and drought. MPKK10.2 physically interacted with MPK6 and MPK3, and phosphorylated the two MAPKs in vivo. Transcriptionally modulating MPKK10.2 influenced MPK6 phosphorylation during rice-Xoc interaction, and MPKK10.2-oe/MPK6-RNAi double mutants showed increased sensitivity to Xoc. MPKK10.2-oe/MPK3-RNAi double mutants showed survival rates similar to those of control plants, although the survival rates of MPKK10.2 transgenic plants changed after drought stress. These results suggest that MPKK10.2 is a node involved in rice response to biotic and abiotic responses by functioning in the cross-point of two MAPK cascades leading to Xoc resistance and drought tolerance.

Drought Resistance

10.1111/tpj.13674

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Citations (111)

Exploring and applying genes to enhance the resistance to Fusarium head blight in wheat

Frontiers in Plant Science (2022)

Haigang Ma Yongjiang Liu Xueyan Zhao Suhong Zhang Hongxiang Ma

Fusarium head blight (FHB) is a destructive disease in wheat worldwide. Fusarium graminearum species complex (FGSC) is the main causal pathogen causing severe damage to wheat with reduction in both grain yield and quality. Additionally, mycotoxins produced by the FHB pathogens are hazardous to the health of human and livestock. Large numbers of genes conferring FHB resistance to date have been characterized from wheat and its relatives, and some of them have been widely used in breeding and significantly improved the resistance to FHB in wheat. However, the disease spreads rapidly and has been severe due to the climate and cropping system changes in the last decade. It is an urgent necessity to explore and apply more genes related to FHB resistant for wheat breeding. In this review, we summarized the genes with FHB resistance and mycotoxin detoxication identified from common wheat and its relatives by using forward- and reverse-genetic approaches, and introduced the effects of such genes and the genes with FHB resistant from other plant species, and host-induced gene silencing (HIGS) in enhancing the resistance to FHB in wheat. We also outlined the molecular rationale of the resistance and the application of the cloned genes for FHB control. Finally, we discussed the future challenges and opportunities in this field.

10.3389/fpls.2022.1026611

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Citations (13)