Virgínia M. F. Gonçalves

Cooperativa de Ensino Superior Politécnico e Universitário

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Maria Elizabeth Tiritan

Cooperativa de Ensino Superior Politécnico e Universitário

Cláudia Ribeiro

Cooperativa de Ensino Superior Politécnico e Universitário

Nuno M. Neves

University of Minho

Helena Ferreira

University of Minho

Rui L. Reis

University of Minho

Ana R. Ribeiro

Universidade do Porto

Sara F. Vieira

University of Minho

Alexandra S. Maia

Cooperativa de Ensino Superior Politécnico e Universitário

Sandra Leal

Cooperativa de Ensino Superior Politécnico e Universitário

Ana Sousa

Cooperativa de Ensino Superior Politécnico e Universitário

Cooperative Institutions

Universidade do Porto

Cooperativa de Ensino Superior Politécnico e Universitário

Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde

University of Minho

Rede de Química e Tecnologia

University of Aveiro

Unidade em Ciências Biomoleculares Aplicadas

Centro Interdisciplinar de Investigação Marinha e Ambiental

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto

University of Lisbon

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Aqueous Extracts of Fish Roe as a Source of Several Bioactive Compounds

Separations (2022)

Marta Guedes Virgínia M. F. Gonçalves Maria Elizabeth Tiritan Rui L. Reis Helena Ferreira

Regular consumption of seafood and, in particular, fish has been associated with important health benefits. A fish product that has been increasingly included in the human nutrition is roe. Despite its nutritional value has been established (fatty acid profile and protein content), the knowledge of the composition of its aqueous extracts is still limited. This work describes the bioactive compounds profile in the roe-derived aqueous extracts of three different marine species (sardine, horse mackerel and sea bass) using a method based on liquid chromatography coupled to high-resolution mass spectrometry with an electrospray ionisation source (LC-ESI/HRMS). The presence of substances with well-known nutritional and functional properties (e.g., antioxidant and anti-inflammatory properties) was demonstrated, namely essential amino acids (e.g., taurine), peptides (e.g., anserine and carnosine), B-group vitamins (e.g., nicotinamide) and gadusol. Therefore, roe-derived aqueous extracts are excellent sources of bioactive compounds and may be used as a font of functional components for several medical and veterinary applications.

Sea bass

Nutraceutical

10.3390/separations9080210

Cite

Citations (3)

Unveil the toxicity induced on early life stages of zebrafish (Danio rerio) exposed to 3,4-methylenedioxymethamphetamine (MDMA) and its enantiomers

The Science of The Total Environment (2024)

Ondina Ribeiro Luís Félix Cláudia Ribeiro Mónica Torres-Ruíz Maria Elizabeth Tiritan

Danio

MDMA

Ecstasy

10.1016/j.scitotenv.2024.176906

Cite

Citations (0)

Toxicity of butylone and its enantiomers to Daphnia magna and its degradation/toxicity potential using advanced oxidation technologies

Aquatic Toxicology (2024)

Ana Rita Carvalho Ana M. Morão Virgínia M. F. Gonçalves Maria Elizabeth Tiritan Ana M. Gorito

Butylone (BTL) is a chiral synthetic cathinone available as a racemate and reported as contaminant in wastewater effluents. However, there are no studies on its impact on ecosystems and possible enantioselectivity in ecotoxicity. This work aimed to evaluate: (i) the possible ecotoxicity of BTL as racemate or its isolated (R)- and (S)- enantiomers using Daphnia magna; and (ii) the efficiency of advanced oxidation technologies (AOTs) in the removal of BTL and reduction of toxic effects caused by wastewaters. Enantiomers of BTL were obtained by liquid chromatography (LC) using a chiral semi-preparative column. Enantiomeric purity of each enantiomer was > 97 %. For toxicity assessment, a 9-day sub-chronic assay was performed with the racemate (at 0.10, 1.0 or 10 μg L

Daphnia magna

Ecotoxicity

10.1016/j.aquatox.2024.106906

Cite

Citations (4)

Análise de resíduos de disparo orgânicos, de armas de fogo, por cromatografia

RevSALUS - Revista Científica da Rede Académica das Ciências da Saúde da Lusofonia (2022)

Beatriz Nogueira Siqueira e Silva Cláudia Ribeiro Virgínia M. F. Gonçalves Cristina Couto

10.51126/revsalus.v4isup.293

Cite

Citations (1)

Liquid chromatographic methods for the therapeutic drug monitoring of methotrexate as clinical decision support for personalized medicine: A brief review

Biomedical Chromatography (2017)

Mónica Francisco Silva Cláudia Ribeiro Virgínia M. F. Gonçalves Maria Elizabeth Tiritan Áurea Lima

Methotrexate (MTX) is an antifolate drug used for several diseases. Depending on the disease, MTX can be administered at low dose (LDMTX) in some autoimmune diseases, like rheumatoid arthritis, or at high dose (HDMTX) in some cancers, such as acute lymphoblastic leukemia. After absorption, MTX is metabolized in the liver to 7-hydroxymethotrexate and in the intestine to 2,4-diamino-N10-methylpteroic acid (DAMPA). Moreover, inside red blood cells, MTX is converted to active metabolites, MTX polyglutamates (MTXPGs), contributing to its pharmacodynamics. Owing to its narrow therapeutic range, and inter- and intra-patient variability, either noneffectiveness and/or toxicity may occur. Because of the existence of a relationship between drug therapeutic outcome and its systemic concentration, therapeutic drug monitoring (TDM) may ensure the effectiveness and safety of MTX use. In order to monitor the optimization of patient clinical response profile, several analytical methods have been described for TDM in biological samples. These include liquid chromatography (LC) coupled with ultraviolet detection, fluorescence detection or mass spectrometry, each one presenting advantages and drawbacks. This paper reviews the most commonly used techniques for sample preparation and critically discusses the current LC methods applied for the TDM of MTX in biological samples, at LDMTX and HDMTX.

Therapeutic Drug Monitoring

Antifolate

Therapeutic index

Pharmacodynamics

Fluorescence polarization immunoassay

10.1002/bmc.4159

Cite

Citations (32)

Amphetamine-like substances and synthetic cathinones in Portuguese wastewater influents: Enantiomeric profiling and role of suspended particulate matter

Forensic Science International (2024)

Ivan M. Langa Ana R. Ribeiro Nuno Ratola Virgínia M. F. Gonçalves Maria Elizabeth Tiritan

Wastewater based epidemiology (WBE) has been used worldwide to estimate drug consumption routinely. Even though WBE provides valuable data to support legal and health interventions associated to drug use, monitoring studies in Portuguese wastewaters are scarce. Hence, this work aimed to estimate the consumption of some conventional abuse and illicit drugs such as amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxymethamphetamine (MDMA), and the synthetic cathinones buphedrone (BPD), butylone (BTL), 3,4-dimethylmethcathinone (3,4-DMMC) and 3-methylmethcathinone (3-MMC), considering not only the liquid phase, but also the suspended particulate matter (SPM). Moreover, the enantiomeric profiling of the samples was studied, exploring for the first time the possible enantioselective sorption of these drugs onto SPM. For that, 24 h composite raw wastewaters were collected from a conventional wastewater treatment plant (WWTP) in Portugal. After extraction, the liquid phase and SPM extracts were derivatized with an enantiomerically pure reagent and then, analysed using a gas chromatography-mass spectrometry (GC-MS) analytical method. The results showed a low and non-enantioselective adsorption to SPM at environmental relevant levels. Only (S)-AMP was detected in two SPM samples, whereas AMP, MAMP, MDMA, BPD, and 3,4-DMMC were detected in the liquid phase. AMP was the most frequently found drug with an estimated load up to 166.0 mg day

Profiling (computer programming)

10.1016/j.forsciint.2024.112128

Cite

Citations (2)

Microfluidic mixing system for precise PLGA-PEG nanoparticles size control

Nanomedicine Nanotechnology Biology and Medicine (2021)

Sara Gimondi Carlos F. Guimarães Sara F. Vieira Virgínia M. F. Gonçalves Maria Elizabeth Tiritan

PLGA

Flow focusing

10.1016/j.nano.2021.102482

Cite

Citations (30)

Chromatographic Methods for Detection and Quantification of Pyrrolizidine Alkaloids in Flora, Herbal Medicines, and Food: An Overview

Critical Reviews in Analytical Chemistry (2023)

Ana Sousa Cláudia Ribeiro Virgínia M. F. Gonçalves Inês Pádua Sandra Leal

AbstractPyrrolizidine alkaloids (PAs) are natural toxins produced by some plants that gained special interest due to their potential hazardous effects in humans and animals. These substances have been found in wild flora, herbal medicines and food products raising health concerns. Recently, maximum concentration levels of PAs were established for some food products; however, maximum daily intake frequently surpasses the upper limit set by the competent authorities posing a health risk. Given the scarcity or absence of occurrence data on PAs in many products, there is an urgent need to measure their levels and establish safety intake levels. Analytical methods have been reported to detect and quantify PAs in different matrices. The commonly used chromatographic methodologies provides accurate and reliable results. Analytical methods include diverse steps as extraction and sample preparation procedures that are critical for sensitivity and selectivity of the analytical method. Great efforts have been directed toward optimization of extraction procedures, clean up and chromatographic conditions to improve recovery, reduce matrix effects, and achieve low limits of detection and quantification. Therefore, this paper aims to give a general overview about the occurrence of PAs in flora, herbal medicines, and foodstuff; and discuss the different chromatographic methodologies used for PAs analysis, namely extraction and sample preparation procedures and chromatographic conditions.Keywords: Chromatographyfood analysisfood contaminantsfood safetyherbal medicines Disclosure statementThe authors report there are no competing interests to declare.Additional informationFundingThis work was supported by the CESPU – Cooperativa de Ensino Superior Politécnico e Universitário under Grant SPAinT-GI2-CESPU-2022.

Flora

10.1080/10408347.2023.2218476

Cite

Citations (5)

Structural characterization of the acetylated heteroxylan from the natural hybrid Paulownia elongata/Paulownia fortunei

Carbohydrate Research (2007)

Virgínia M. F. Gonçalves Dmitry V. Evtuguin M. Rosário M. Domingues

Paulownia

10.1016/j.carres.2007.11.002

Cite

Citations (60)

Evaluation of Antitumor Activity of Xanthones Conjugated with Amino Acids

International Journal of Molecular Sciences (2024)

Flávia Barbosa Joana Araújo Virgínia M. F. Gonçalves Andreia Palmeira Andrea Cunha

Cancer is a complex disease characterized by several alterations, which confer, to the cells, the capacity to proliferate uncontrollably and to resist cellular death. Multiresistance to conventional chemotherapy drugs is often the cause of treatment failure; thus, the search for natural products or their derivatives with therapeutic action is essential. Chiral derivatives of xanthones (CDXs) have shown potential inhibitory activity against the growth of some human tumor cell lines. This work reports the screening of a library of CDXs, through viability assays, in different cancer cell lines: A375-C5, MCF-7, NCI-H460, and HCT-15. CDXs’ effect was analyzed based on several parameters of cancer cells, and it was also verified if these compounds were substrates of glycoprotein-P (Pgp), one of the main mechanisms of resistance in cancer therapy. Pgp expression was evaluated in all cell lines, but no expression was observed, except for HCT-15. Also, when a humanized yeast expressing the human gene MDR1 was used, no conclusions could be drawn about CDXs as Pgp substrates. The selected CDXs did not induce significant differences in the metabolic parameters analyzed. These results show that some CDXs present promising antitumor activity, but other mechanisms should be triggered by these compounds.

P-glycoprotein

Viability assay

10.3390/ijms25042121

Cite

Citations (0)