Brian S. Sproat

Research Field

Cite

Citations (0)

RNA Synthesis Using 2'-<I>O</I>-(<I>Tert</I>-Butyldimethylsilyl) Protection

Humana Press eBooks (2004)

This chapter enables the reader to carry out the solid-phase synthesis of ribonucleic acid (RNA) using beta-cyanoethyl phosphoramidite chemistry combined with tert-butyldimethylsilyl protection of the ribose 2'-hydroxyl group. Phosphoramidite monomers are activated with 5-benzylmercapto-1H-tetrazole enabling fast and highly efficient coupling to the 5'-hydroxyl group of the support-bound oligonucleotide. On completion of the synthesis, the stepwise deprotection of the nucleobase, phosphate, and ribose protecting groups is carried out using optimized protocols. Subsequently the various high-pressure (performance) liquid chromatography (HPLC) procedures are described enabling the purification and analysis of the RNA. For this purpose anion-exchange and reversed-phase HPLC are used singly or in combination according to the final purity requirement of the RNA.

10.1385/1-59259-823-4:017

Cite

Citations (20)

The synthesis of protected 5′-mercapto-2′,5′-dideoxyribonucleoside-3′-0-phosphorainidites; uses of 5′-mercapto-oligodeoxyribonucleotides

Nucleic Acids Research (1987)

Brian S. Sproat Barbro Beijer Peter Rider Philippe Neuner

The syntheses of the four novel, base protected 5'-(S-triphenylmethyl)mercapto-2',5'-dideoxyribonucleoside-3 '-O-(2-cyanoethyl N,N-diisopropylphosphoramidites) are described. These compounds have been used to prepare 5'-(S-triphenylmethyl) mercapto-oligodeoxyribonucleotides, which are readily purified by reversed phase h.p.l.c., owing to the highly lipophilic trityl group. After cleavage of the S-trityl group by silver or mercuric ions, the free thiol moiety can be coupled to a wide variety of reagents, generating very useful probes. Fluorescent labelled 5'-mercapto-oligodeoxyribonucleotides are being used for automated DNA sequencing without radioactivity, and heavy metal labelled 5'-mercapto-oligonucleotides will be used in X-ray crystallography.

Moiety

Cleavage (geology)

Thiol

10.1093/nar/15.12.4837

Cite

Citations (58)

Hammerhead ribozymes with cleavage site specificity for NUH and NCH display significant anti-hepatitis C viral effect in vitro and in recombinant HepG2 and CCL13 cells

Journal of Hepatology (2005)

Maria-Angeles Gonzalez-Carmona Sabine Schüssler Matthias Serwe Michael Alt János Ludwig

Hammerhead ribozyme

Cleavage (geology)

10.1016/j.jhep.2005.10.022

Cite

Citations (17)

Studying pre-mRNA splicing using antisense 2-OMe RNA oligonucleotides

Molecular Biology Reports (1990)

Angus I. Lamond Silvia M.L. Barabino Benjamin J. Blencowe Brian S. Sproat Ursula Ryder

Post-transcriptional modification

10.1007/bf00360473

Cite

Citations (9)

Biophysical characterization of hyper-branched polyethylenimine-graft- polycaprolactone-block-mono-methoxyl-poly(ethylene glycol) copolymers (hy-PEI-PCL-mPEG) for siRNA delivery

Journal of Controlled Release (2011)

Yu Liu Olga Samsonova Brian S. Sproat Olivia M. Merkel Thomas Kissel

Polycaprolactone

Polyethylenimine

Zeta potential

10.1016/j.jconrel.2011.04.017

Cite

Citations (70)

PEGylation of biodegradable dextran nanogels for siRNA delivery

European Journal of Pharmaceutical Sciences (2010)

Broes Naeye Koen Raemdonck Katrien Remaut Brian S. Sproat J. Demeester

PEGylation

Nanogel

10.1016/j.ejps.2010.04.010

Cite

Citations (92)

¹⁸O-isotope shifts on the³¹P nuclear magnetic resonance of adenosine-5′-phosphate and inorganic phosphate

Journal of the Chemical Society Chemical Communications (1978)

Gordon Lowe Brian S. Sproat

An isotope shift of 0·025 ± 0·003 p.p.m. to higher field is observed in the 31P n.m.r. spectrum of adenosine-5′-[18O]phosphate; for inorganic phosphate a shift to higher field of 0·020 ± 0·001 p.p.m. is observed for each 18O atom incorporated.

Kinetic isotope effect

10.1039/c39780000565

Cite

Citations (50)

A simple method for the synthesis of 2′-O-alkylguanosine derivatives

Bioorganic & Medicinal Chemistry Letters (1997)

Morten Grøtli Mark E. Douglas Barbro Beijer Ramón Eritja Brian S. Sproat

10.1016/s0960-894x(97)00024-3

Cite

Citations (16)

Mapping U2 snRNP--pre-mRNA interactions using biotinylated oligonucleotides made of 2′-OMe RNA.

The EMBO Journal (1989)