Matthew McMichael

Naples Anesthesia & Physician Associates

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Bill Roschek

Naples Anesthesia & Physician Associates

Ryan C. Fink

University of Pennsylvania

Randall S. Alberte

Naples Anesthesia & Physician Associates

Dan Li

National University of Singapore

Dan Li

East China University of Science and Technology

R. Fink

Friedrich-Alexander-Universität Erlangen-Nürnberg

Sharon Isern

University of Florida Health

Robert Smith

Guy's and St Thomas' NHS Foundation Trust

S. F. Michael

Florida Gulf Coast University

Christine M. Tower

Naples Anesthesia & Physician Associates

Cooperative Institutions

Florida Gulf Coast University

Naples Anesthesia & Physician Associates

Sun Yat-sen University

National University of Singapore

Sichuan Agricultural University

Soochow University

Second Affiliated Hospital of Soochow University

Shaanxi University of Science and Technology

Shanghai Jiao Tong University

Suzhou Research Institute

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Nettle extract (Urtica dioica) affects key receptors and enzymes associated with allergic rhinitis

Phytotherapy Research (2009)

Bill Roschek Ryan C. Fink Matthew McMichael Randall S. Alberte

Abstract A nettle ( Urtica dioica ) extract shows in vitro inhibition of several key inflammatory events that cause the symptoms of seasonal allergies. These include the antagonist and negative agonist activity against the Histamine‐1 (H 1 ) receptor and the inhibition of mast cell tryptase preventing degranulation and release of a host of pro‐inflammatory mediators that cause the symptoms of hay fevers. The nettle extract also inhibits prostaglandin formation through inhibition of Cyclooxygenase‐1 (COX‐1), Cyclooxygenase‐2 (COX‐2), and Hematopoietic Prostaglandin D 2 synthase (HPGDS), central enzymes in pro‐inflammatory pathways. The IC 50 value for histamine receptor antagonist activity was 251 (±13) µg mL −1 and for the histamine receptor negative agonist activity was 193 (±71) µg mL −1 . The IC 50 values for inhibition of mast cell tryptase was 172 (±28) µg mL −1 , for COX‐1 was 160 (±47) µg mL −1 , for COX‐2 was 275 (±9) µg mL −1 , and for HPGDS was 295 (±51) µg mL −1 . Through the use of DART TOF‐MS, which yields exact masses and relative abundances of compounds present in complex mixtures, bioactives have been identified in nettle that contribute to the inhibition of pro‐inflammatory pathways related to allergic rhinitis. These results provide for the first time, a mechanistic understanding of the role of nettle extracts in reducing allergic and other inflammatory responses in vitro . Copyright © 2009 John Wiley & Sons, Ltd.

Urtica dioica

Prostaglandin D2

10.1002/ptr.2763

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Elderberry flavonoids bind to and prevent H1N1 infection in vitro

Phytochemistry (2009)

Bill Roschek Ryan C. Fink Matthew McMichael Dan Li Randall S. Alberte

10.1016/j.phytochem.2009.06.003

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Pro-Inflammatory Enzymes, Cyclooxygenase 1, Cyclooxygenase 2, and 5-Lipooxygenase, Inhibited by Stabilized Rice Bran Extracts

Journal of Medicinal Food (2009)

Bill Roschek Ryan C. Fink Dan Li Matthew McMichael Christine M. Tower

Rice bran, the outer bran and germ of the kernel and a by-product of rice milling, is rich in phytonutrients but has been underutilized because of lipid content instability. New methods for the processing of rice bran have yielded a stabilized form that is increasingly used in foods and dietary supplements. Recent studies have documented a role for stabilized rice bran (SRB) in treating diabetes and arthritis, although little is known of the bioactive compounds that impart these health benefits. Here we characterize the chemical composition of three extracts of SRB and identify the functional bioactives contributing to the inhibitory properties against three key pro-inflammatory enzymes (cyclooxygenase [COX] 1, COX2, and 5-lipoxygenase [5-LOX]) that control the inflammatory cascade involved in impaired joint health, pain, and arthritis. One extract (SRB-AI) demonstrated significant COX1 and COX2 inhibitory activities with 50% inhibitory concentration (IC50) values for COX1 and COX2 of 305 and 29 μg/mL, respectively, but no 5-LOX inhibition. The second extract (SRB-AII) inhibited COX1, COX2, and 5-LOX with IC50 values of 310, 19, and 396 μg/mL, respectively. The third extract (SRB-AIII), a blend of SRB-AI and SRB-AIII, inhibited COX1, COX2, and 5-LOX with respective IC50 values of 48, 11, and 197 μg/mL. Analysis of the extracts by direct analysis in real time time of flight-mass spectrometry revealed that SRB-AI, SRB-AII, and SRB-AIII contain over 620, 770, and 810 compounds, respectively. Of these, 17 were identified as key bioactives for COX and/or LOX inhibition. These SRB extracts have applications for functional foods and dietary supplements for control of inflammation and joint health.

Functional food

10.1089/jmf.2008.0133

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In vitro inhibition of dengue virus entry by p-sulfoxy-cinnamic acid and structurally related combinatorial chemistries

Antiviral Research (2008)

Craig R. Rees Joshua M. Costin R. Fink Matthew McMichael Krystal A. Fontaine

IC50

Cinnamic acid

Cytopathic effect

10.1016/j.antiviral.2008.05.007

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Citations (60)