G. Melyan

National Academy of Sciences of Armenia

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O. Failla

University of Milan

D. Maghradze

Caucasus University

K. Margaryan

Yerevan State University

Erika Maul

Julius Kühn-Institut

Yu. Ts. Martirosyan

Russian Academy of Sciences

Rouben Aroutiounian

Yerevan State University

Mirza Musayev

Azerbaijan National Academy of Sciences

Kima Dangyan

Armenian National Agrarian University

Aghvan Sahakyan

Armenian National Agrarian University

V. Salimov

Quality Research

Cooperative Institutions

Armenian National Agrarian University

National Academy of Sciences of Armenia

University of Milan

Agricultural University of Georgia

Yerevan State University

Diversité, adaptation et développement des plantes

Scientific Center of Zoology and Hydroecology

Agrobiotechnology Institute

National Institute of Grape and Wine "Magarach"

Genetic Improvement and Adaptation of Mediterranean and Tropical Plants

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Preliminary study of Armenian grapevines phenolic contents

Vitis (2015)

K. Margaryan Rouben Aroutiounian G. Melyan O. Failla

Armenian

10.5073/vitis.2015.54.special-issue.155-156

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Effect of biofertilizers on the content of bioactive components of common bean under open field and greenhouse cultivation conditions

Bioactive Compounds in Health and Disease - Online ISSN 2574-0334 Print ISSN 2769-2426 (2024)

Gohar Kirakosyan G. Melyan Vitya Vardanyan Karine Sarikyan

Background: Biofertilizers are important in improving the qualitative and biochemical properties of plants by increasing the quality of the soil, which is directly related to obtaining quality and functional food. Their use is particularly valuable in the cultivation of common beans, which serve as an alternative protein source, that are rich in micronutrients, antioxidants, and vitamins, and possess many medicinal values. By enhancing these qualities through biofertilizer application, common beans can become an important functional food in people’s daily diets. Objective: The main objective of the study was to evaluate the effectiveness of biofertilizer application in the context of improving food functionality on the content of bioactive components (dry matter, total sugars, ascorbic acid, total proteins) of green bean pods and grains. Methods: The study evaluated the effects of three biofertilizers —Fulvomix and Ecobiofid+ (of Armenian origin) and Biomix 1 (of Indian origin)—on the biomorphological, biochemical properties and functional processes of bean crops. Fertilizers were applied during pre-sowing treatments, early intensive growth, and fruiting stages. The experiments were carried out in both open fields and greenhouses, with four repetitions on experimental plots measuring 20 square meters each. Soil analysis, along with the biochemical composition of bean pods and grains, was performed in accordance with the accepted methodology. Results: As a result of the applied biofertilizers, an increase in the content of dry matter, vitamin C, total sugars, and proteins was recorded in green pods and beans. The highest results were recorded with the use of Ecobiofid + biofertilizer․ Conclusion: The application of three biofertilizers contributes to increasing the functional properties of green pods and grain, due to the improvement of the structural quality of the soil, as well as the increase of the indicators of biochemical, bioactive compounds. The positive effect of the applied biofertilizers on the quality indicators and functionality of green pods and grains, as well as on the productivity of the plant, allows the tested semi-fertilizers to be offered to production as a basis for organic farming and the production of healthy, functional food. Keywords: Common beans, Phaseolus vugaris L., biofertilizers, biochemical, organic agriculture, cultivar, seeds.

Biofertilizer

10.31989/bchd.v7i12.1519

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Cytoembryological and morphometric characteristics of some Armenian grape cultivars

Vitis (2015)

Anna Nebish G. Melyan Rouben Aroutiounian

Introduction: The conservation and phenotypic characterization of Armenian grapevine gene pool is the basis for its application in future breeding programs. The cytoembryological analysis and the phenotypic characterization of grapevine male and female gametophytes and of early developed embryos could improve the understanding of genetic mechanisms involved at different stages of reproductive development (PRATT 1971, MULLINS et al. 1992, FERNANDES et al. 2007, NEBISH 2012). Flowering and berry set are important stages of grapevine development, and they could also affect the yield quantity and quality (LONGBOTTOM 2007). Grapevine cultivars are highly heterozygous. They show a large variation in inflorescence size, berry size, shape, weight and colour depending on genotypes and environmental conditions (THIS et al. 2006, CARMONA et al. 2008). The aim of our study was the phenotypic characterization of some Armenian grape cultivars by cytoembryological and carpological analysis. Material and Methods: Ten accessions of Armenian grapevines were analysed including: 5 Vitis vinifera L. cultivars (authochthonous 'Mskhali', 'Garan dmak' and clone of 'Ararati' cultivars, 'Tokun' open pollination of 'Spitak Arakseni', 'Muscat haykakan' open pollination of 'Muscat cherniy'); 3 intraspecific hyvrids ('Berkanush', 'Parvana' and 'Erebuni') and 2 V. vinifera x V. amurensis interspecific hybrids ('Charentsi' and 'Meghrabuyr'). All the accessions were grown in the same collection of the Scientific Center of Viticulture, Fruit-Growing and Wine-Making (Merdzavan, Armenia). In 2013 flowers for cyto-embryological analysis of microand macro-gametophyte, and ripe berries for morphometric studies were collected. For estimation of pollen fertility the acetocarmine staining method have been used. Pollen sterility data were obtained based on the analysis of about 5000 pollen grains for each accession. Concerning cytoembryological investigations, fixation in FAA fixative (formaldehyde – acetic acid – ethanol) were applied. Material was then stained by Mayer's hematoxylin and eosin (HE 1997) by the ImageJ software. Statistical analysis of data was carried out using Student's t-test and 5 % probability was considered significant.

Table grape

Armenian

Wine grape

10.5073/vitis.2015.54.special-issue.175-176

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Impact of aeroponic cultivation and plant growth regulators on the biochemical composition of grapevine leaves

Functional Food Science - Online ISSN 2767-3146 (2024)

G. Melyan Andranik Barseghyan Kima Dangyan Narek Sahakyan Arayik Sajan Vardanyan

Background: Grapevine (Vitis vinifera L.) is a globally important perennial fruit crop, renowned for its economic value and cultural significance. Grape leaves, while often underappreciated, play a vital role in culinary traditions and are valued for their cultural, nutritional, and health benefits. However, the availability of grape leaves is constrained by viticulture practices and chemical treatments that prioritize fruit production over leaf harvesting. This study seeks to compare the biochemical characteristics of grapevine leaves from the cultivar 'Deghin Yerevani' grown from virus-free, tissue-cultured plants under aeroponic and soil conditions within a greenhouse. Objective: To evaluate the impact of aeroponic versus soil-based cultivation and the application of specific growth regulators on the biochemical composition and nutrient content of 'Deghin Yerevani' grapevine leaves, with the goal of optimizing conditions for high-quality leaf production. Materials and Methods: This study, conducted from 2021 to 2023 at the Scientific Center of Agrobiotechnology, ANAU, utilized virus-free in vitro plants of Vitis vinifera L. cv. 'Deghin Yerevani', sourced from the National Grape Field Collection. Plants were cultured in four treatment groups with varying growth media compositions: PGR-free, with Indole-3-acetic acid (IAA), with IAA and Gibberellic acid (GA₃), and with IAA, GA₃, and 6-Benzylaminopurine (BAP). Post-culturing, plants were transferred to either an aeroponic system, which used a misting cycle and maintained controlled environmental conditions, or to a greenhouse with a defined soil mix and monitored moisture levels. Both systems operated for 8 weeks, with each treatment replicated three times. Chlorophylls a and b, ascorbic acid, sugars, and macro- and micronutrient contents were analyzed, and statistical significance was determined using standard error and Student’s t-test (p < 0.05). Results: Grapevine leaves from 'Deghin Yerevani' plants grown in aeroponic systems exhibited significantly higher chlorophyll a, chlorophyll b, and total chlorophyll compared to those grown in soil. Growth regulators, particularly IAA, GA3, and BAP, enhanced chlorophyll content, sugar levels, and Vitamin C across both cultivation methods. Aeroponic systems showed superior results with increased sugar content (5.7 g/100g) and Vitamin C (20.4 mg/100g) compared to soil-based cultivation. Nutrient analysis revealed higher levels of nitrogen, phosphorus, potassium, calcium, and magnesium in aeroponics. Overall, growth regulators and aeroponic cultivation improved the biochemical and nutrient profiles of grapevine leaves. Conclusion: The study demonstrated that aeroponic cultivation and the application of growth regulators significantly enhanced the biochemical composition of grapevine leaves. Higher levels of chlorophyll, sugar, and Vitamin C, along with improved nutrient content, were observed in aeroponic systems compared to soil-based cultivation. These findings underscore the benefits of aeroponics and growth regulators in optimizing grapevine leaf quality, suggesting potential for improved yield and nutritional value in grapevine production. Keywords: 'Deghin Yerevani', grapevine, in vitro propagation, functional foods, Aeroponic Cultivation, Plant Growth Regulators

10.31989/ffs.v4i10.1458

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Armenian grape varieties SSR fingerprinting by using Qiaxcel DNA fragment analysis system

M. Dallakyan S. S. Yesoyan G. Melyan Aleksandr Yesayan Nelli Hovhannisyan

The applicability of QIAxcel System for SSR fingerprinting of grape varieties exploiting the internationally accepted 9 microsatellite markers was investigated. To optimize the allele sizing, a comparative genotyping of seven reference varieties with known sizes of microsatellite alleles, proposed by GENRES 081 project consortium, was performed. Ուսումնասիրվել և գնահատվել է QIAxcel համակարգի կիրառելիությունը խաղողի սորտերի՝ միջազգայնորեն ընդունված 9 միկրոսատելիտային մարկերներով ուսումնասիրությունների համար: Изучена и оценена применяемость системы QIAxcel для микросателлитного генотипироания сортов винограда с применением 9 междунороднопризнанных микросателлитных маркеров. В целях оптимизации определения длины аллелей было проведено сравнительное генотипирование семи стандартных сортов с известной длиной микросателлитных аллелей, предложенных консорцием проекта GENRES 081.

DNA profiling

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Support trees and shrubs for the Eurasian wild grapevine in Southern Caucasus

Annals of Agrarian Science (2018)

Rafael Ocete Rubio Diego Rivera D. Maghradze V. Salimov G. Melyan

A prospecting of habitats and mechanical support host species for the climber Eurasian wild grapevine, Vitis vinifera L. subsp. sylvestris (Gmelin) Hegi, was carried out on 13 natural populations situated along river bank forests, floodplains and colluvial positions in Georgia (Marneuli, Mtskheta and Gori districts, Gardabani Protected area and Lagodekhi Reserve), Armenia (Akhtala and Tavoush regions) and Azerbaijan (Quba region) during survey of 2013. The research demonstrated that Eurasian wild grapevine (Vitis vinifera subsp. sylvestris) is found in Southern Caucasus in a wide variety of habitats always linked to water availability. Punica granatum trees are the commonest mechanical support for wild grapevine in the South Caucasus and Hedera helix often shares the same support trees. However we documented wild grapevines climbing on other 24 different species of trees and large shrubs and, further, 32 associated species. We determined, four different clusters of localities using Structure software and the Weighted Neighbor Joining tree. These clusters are characterized by specific mechanical support and accompanying species. Other vines competing for host with Eurasian wild grapevine belong to the genera Clematis, Hedera, Humulus, Smilax and Vitis ssp.

Liana

10.1016/j.aasci.2018.06.005

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Evaluation of breeding potential of wild grape originating from Armenia

BIO Web of Conferences (2019)

K. Margaryan Erika Maul Z. Muradyan Anahit Hovhannisyan G. Melyan

Crop wild relatives provide a useful source of genetic variation and represent a large pool of genetic diversity for new allelic variation required in breeding programs. Armenia is an important center of origin both for cultivated Vitis vinifera ssp. s ativa and wild Vitis vinifera ssp. sylvestris. Owing to recent prospection in Armenian woods and river floodplains many forms of wild grapevine were discovered and inventoried, which is an important prerequisite to unlock their breeding potential in the future. The fact that some genotypes of V. sylvestris can withstand the diseases is likely to be due to a more efficient basal immunity. The overall goal of the proposed research was to characterize the diversity of V. sylvestris from Armenia with respect to its capacity for stilbene biosynthesis, which might be exploited as genetic resource for resistance breeding. The realized research stimulates the recovery, characterization and preservation of wild grape germplasm, presently at risk of extinction. The recovery and characterization of wild genotypes will be the base of selection of genetic traits important in breeding programs for the generation of biotic and changing climate tolerant grapevine varieties and rootstocks, both necessary for the future of viticulture in Armenia and in Europe.

Germ plasm

Breeding program

10.1051/bioconf/20191501006

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POLYPHENOLIC PROFILES DETECTED IN ARMENIAN WINE AND TABLE GRAPE CULTIVARS

Acta Horticulturae (2014)

K. Margaryan R.M. Aroutionian G. Melyan O. Failla

Armenian

Table grape

Wine grape

Table (database)

10.17660/actahortic.2014.1032.18

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Micropropagation of grapevine (Vitis vinifera L.) seedless cultivar 'Parvana' through lateral bud development

G. Melyan A. Sahakyan Ani Harutyunyan

Factors affecting successful establishment in vitro, rapid proliferation, rooting and acclimatization of grapevine seedless cultivation 'Parvana' were studied. A treatment combination of 2.0 % calcium hypochlorite for 7 minutes plus 70 % ethanol for 3 minutes was found to be effective with 75.0 % of aseptic culture establishment in vitro. The highest number of new microshoots (4.5) was obtained in a culture medium (Gambourg's B5 modification) supplemented with 0.6 mg∙L-1 BAP + 0.2 mg∙L-1 KIN + 0.5 mg∙L-1 GA3. Presence of GA3 in combination with BAP and KIN promoted elongation of shoots. A concentration of 0.4 mg/l IAA in half strength of MS (Murashige and Skoog) proved to be the best for rooting (84.4 % root induction, 4.66 roots with 7.9 cm length). In vitro rooted plantlets were successfully acclimatized, with 82.2 % survival rate in plastic pots containing garden soil, sand and peat moss(1:1:1). In this study an efficient micro propagation technique of grapevine seedless cultivar 'Parvana' was successfully achieved through lateral bud culture. Such a technique would be useful for large scale plant production and in vitro conservation.

10.5073/vitis.2015.54.special-issue.253-255

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Molecular investigation of Caucasian and Eastern European grapevine cultivars (V. vinifera L.) by microsatellites

Gabriella De Lorenzis D. Maghradze B. Biagini G. Simone Di Lorenzo G. Melyan

Grapevine (V. vinifera L.) is one of the most widely cultivated species of agricultural interest. The domestication of wild grapes starting in the Neolithic Age, took place in the Near East area. The aim of this study was the genotyping of germplasm coming from Armenia, Azerbaijan, Georgia and Moldova by SSR markers in order to investigate the genetic relationships among samples along the East-to-West dissemination route of grapevine after the domestication. Based on the descriptive statistics Azerbaijani samples appeared having the highest genetic diversity. PCoA and STRUCTURE analysis revealed three groups: i) Central European group; ii) a group reuniting cultivars coming from Armenia, Georgia and Moldova; iii) the group of Azerbaijani cultivars (94%). The analysis of genetic relationships in our dataset provided evidence of connection among cultivars included in the proles pontica and proles orientalis and geographical origin and human uses as well.

Germ plasm

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