Dane C. Jasperson

Center for Grain and Animal Health Research

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William C. Wilson

Center for Grain and Animal Health Research

Natasha N. Gaudreault

Kansas State University

Eileen N. Ostlund

Animal and Plant Health Inspection Service

Donna J. Johnson

Animal and Plant Health Inspection Service

Mark G. Ruder

University of Georgia

Jüergen A. Richt

Kansas State University

David E. Stallknecht

University of Georgia

Barbara S. Drolet

United States Department of Agriculture

Dana Mitzel

United States Department of Agriculture

Dustin A. Swanson

Center for Grain and Animal Health Research

Cooperative Institutions

Agricultural Research Service

United States Department of Agriculture

Kansas State University

Center for Grain and Animal Health Research

University of California, Davis

University of Pretoria

Animal and Plant Health Inspection Service

National Animal Disease Center

Onderstepoort Veterinary Academic Hospital

Cornell University

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Corrigendum to “Development of a Rift Valley fever real-time RT-PCR assay that can detect all three genome segments” [J. Virol. Methods 193 (2) (2014) 426–431]

Journal of Virological Methods (2014)

William C. Wilson Marco Romito Dane C. Jasperson Hana M. Weingartl Yatinder S. Binepal

Rift Valley Fever

10.1016/j.jviromet.2014.05.005

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Citations (2)

Molecular evolution of American field strains of bluetongue and epizootic haemorrhagic disease viruses.

PubMed (2016)

William C. Wilson Natasha N. Gaudreault Dane C. Jasperson Donna J. Johnson Eileen N. Ostlund

Recent Orbivirus occurrences in the Americas have been investigated using whole genome amplification and sequencing followed by phylogenetic analysis. The bluetongue virus (BTV) and epizootic haemorrhagic disease virus (EHDV) whole genomes were amplified without prior sequence knowledge and deep sequenced. This technology was applied to evaluate BTV‑3 isolates spanning 4 decades from Florida, Arkansas, Mississippi, South Dakota, Central America, and the Caribbean basin. The results of the dataset analysis are consistent with the hypothesis that these viruses were introduced into the United States from Central America and the Caribbean basin. A similar analysis has been performed on a recent BTV‑2 isolate from California. It indicates that the BTV‑2 strain was likely introduced into Florida and then moved South to the Caribbean and West to California. A historical (1955‑2012) molecular characterisation of EHDV strains was also completed, and subsequently used as reference sequence for comparison of genomes from recent 2012 cattle isolates associated with clinical disease. Finally, this analysis was performed on BTV‑11 isolated from 2 canine cases and demonstrated that the genome sequences of the virus isolates from these cases were almost identical. These studies indicate the value of this technology in understanding virus epidemiology and ecology.

Epizootic

Orbivirus

Molecular Epidemiology

10.12834/vetit.555.2627.1

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Citations (7)

Whole genome sequencing and phylogenetic analysis of Bluetongue virus serotype 2 strains isolated in the Americas including a novel strain from the western United States

Journal of Veterinary Diagnostic Investigation (2014)

Natasha N. Gaudreault Christie Mayo Dane C. Jasperson Beate Crossley Richard E. Breitmeyer

Bluetongue is a potentially fatal arboviral disease of domestic and wild ruminants that is characterized by widespread edema and tissue necrosis. Bluetongue virus (BTV) serotypes 10, 11, 13, and 17 occur throughout much of the United States, whereas serotype 2 (BTV-2) was previously only detected in the southeastern United States. Since 1998, 10 other BTV serotypes have also been isolated from ruminants in the southeastern United States. In 2010, BTV-2 was identified in California for the first time, and preliminary sequence analysis indicated that the virus isolate was closely related to BTV strains circulating in the southeastern United States. In the current study, the whole genome sequence of the California strain of BTV-2 was compared with those of other BTV-2 strains in the Americas. The results of the analysis suggest co-circulation of genetically distinct viruses in the southeastern United States, and further suggest that the 2010 western isolate is closely related to southeastern strains of BTV. Although it remains uncertain as to how this novel virus was translocated to California, the findings of the current study underscore the need for ongoing surveillance of this economically important livestock disease.

Strain (injury)

Orbivirus

10.1177/1040638714536902

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Citations (14)

Susceptibility of midge and mosquito vectors to SARS-CoV-2 by natural route of infection

bioRxiv (Cold Spring Harbor Laboratory) (2020)

Velmurugan Balaraman Barbara S. Drolet Natasha N. Gaudreault William C. Wilson Jeana Owens

Abstract SARS-CoV-2 is a recently emerged, highly contagious virus and the cause of the current pandemic. It is a zoonotic virus, although its animal origin is not clear yet. Person-to-person transmission occurs by inhalation of infected droplets and aerosols, or by direct contact with contaminated fomites. Arthropods transmit numerous viral, parasitic, and bacterial diseases; however, the potential role of arthropods in SARS-CoV-2 transmission is not fully understood. Thus far, a few studies have demonstrated that SARS-CoV-2 replication is not supported in cells from certain insect species nor in certain species of mosquitoes after intrathoracic inoculation. In this study, we expanded the work of SARS-CoV-2 susceptibility to biting insects after ingesting a SARS-CoV-2infected blood meal. Species tested included Culicoides sonorensis biting midges, as well as Culex tarsalis and Culex quinquefasciatus mosquitoes, all known biological vectors for numerous RNA viruses. Arthropods were allowed to feed on SARS-CoV-2 spiked blood and at various time points post infection analyzed for the presence of viral RNA and infectious virus. Additionally, cell lines derived from C. sonorensis (W8a), Ae. aegypti (C6/36), Cx. quinquefasciatus (HSU), and Cx. tarsalis (CxTrR2) were tested for SARS-CoV-2 susceptibility. Our results indicate that none of the biting insects, nor the insect cell lines support SARS-CoV-2 replication. We conclude, that biting insect do not pose a risk for transmission of SARS-CoV-2 to humans or animals following a SARS-CoV-2 infected blood meal.

Culex quinquefasciatus

Biting

Blood meal

Ceratopogonidae

Culicoides

10.1101/2020.09.29.317289

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Citations (3)

Molecular evolution of epizootic hemorrhagic disease viruses in North America based on historical isolates using motif fingerprints

Virus Genes (2016)

William C. Wilson Mark G. Ruder Dane C. Jasperson Timothy P. L. Smith Pejman Naraghi‐Arani

Epizootic

Motif (music)

Medical microbiology

10.1007/s11262-016-1332-z

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Citations (9)

An improved Real-Time Polymerase Chain Reaction for the Simultaneous Detection of All Serotypes of Epizootic Hemorrhagic Disease Virus

Journal of Veterinary Diagnostic Investigation (2010)

Alfonso Clavijo Feng Sun Thomas Lester Dane C. Jasperson William C. Wilson

Epizootic hemorrhagic disease virus (EHDV) is a significant pathogen of wild and sometimes domestic ungulates worldwide. Rapid and reliable methods for virus detection and identification play an essential part in the control of epizootic hemorrhagic disease (EHD). In the present study, a 1-step real-time polymerase chain reaction (PCR) group-specific assay was developed. The assay detects genome segment 5 (NS1) from all of the 8 serotypes of EHDV. Assay sensitivity was evaluated relative to a conventional gel-based nested PCR using cell culture–derived virus and diagnostic samples from clinically affected white-tailed deer ( Odocoileus virginianus). The assay reliably amplified the NS1 gene from any of the EHDV strains tested, including isolates from each of the 8 EHDV serotypes. No cross-reactions were detected when all 24 serotypes of Bluetongue virus, a closely related member of the genus Orbivirus, were tested. A panel of 76 known EHDV-positive clinical samples was used to compare the performance of the assay relative to a previously reported real-time PCR assay. Results indicated that there was no statistically significant difference between the threshold cycle values obtained with both assays. A collection of 178 diagnostic samples submitted for EHD diagnosis was also used for test evaluation. The assay could be applied for rapid detection of EHDV in clinical samples from susceptible ruminants during an outbreak of the disease. In addition, this PCR assay has the benefits of being reliable and simple and could provide a valuable tool for studying the epidemiology of EHDV infection in susceptible ruminants by facilitating the detection of EHDV, regardless of the serotype.

Orbivirus

Odocoileus

Epizootic

10.1177/104063871002200414

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Citations (37)

Identification and evaluation of antivirals for Rift Valley fever virus

Veterinary Microbiology (2019)

Yuekun Lang Yonghai Li Dane C. Jasperson Jamie Henningson Jinhwa Lee

Rift Valley Fever

Mitoxantrone

10.1016/j.vetmic.2019.01.027

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Citations (13)

Transfection of Culicoides sonorensis biting midge cell lines with Wolbachia pipientis

Parasites & Vectors (2019)

Arnab Ghosh Dane C. Jasperson Lee W. Cohnstaedt Corey L. Brelsfoard

Abstract Background Biting midges of the genus Culicoides vector multiple veterinary pathogens and are difficult to control. Endosymbionts particularly Wolbachia pipientis may offer an alternative to control populations of Culicoides and/or impact disease transmission in the form of population suppression or replacement strategies. Methods Culicoides sonorensis cell lines were transfected with a Wolbachia infection using a modified shell vial technique. Infections were confirmed using PCR and cell localization using fluorescent in situ hybridization (FISH). The stability of Wolbachia infections and density was determined by qPCR. qPCR was also used to examine immune genes in the IMD, Toll and JACK/STAT pathways to determine if Wolbachia were associated with an immune response in infected cells. Results Here we have transfected two Culicoides sonorensis cell lines (W3 and W8) with a Wolbachia infection ( w albB) from donor Aedes albopictus Aa23 cells. PCR and FISH showed the presence of Wolbachia infections in both C. sonorensis cell lines. Infection densities were higher in the W8 cell lines when compared to W3. In stably infected cells, genes in the immune Toll, IMD and JAK/STAT pathways were upregulated, along with Attacin and an Attacin-like anti-microbial peptides. Conclusions The successful introduction of Wolbachia infections in C. sonorensis cell lines and the upregulation of immune genes, suggest the utility of using Wolbachia for a population replacement and/or population suppression approach to limit the transmission of C. sonorensis vectored diseases. Results support the further investigation of Wolbachia induced pathogen inhibitory effects in Wolbachia -infected C. sonorensis cell lines and the introduction of Wolbachia into C. sonorensis adults via embryonic microinjection to examine for reproductive phenotypes and host fitness effects of a novel Wolbachia infection.

Culicoides

10.1186/s13071-019-3716-0

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Citations (14)

Evaluation of 2012 US EHDV-2 outbreak isolates for genetic determinants of cattle infection

Journal of General Virology (2019)

Erin E. Schirtzinger Dane C. Jasperson Mark G. Ruder David E. Stallknecht Christopher Chase

Following a summer of severe drought and abnormally high temperatures, a major outbreak of EHDV occurred during 2012 in the USA. Although EHDV-1, -2 and -6 were isolated, EHDV-2 was the predominant virus serotype detected during the outbreak. In addition to large losses of white-tailed deer, the Midwest and northern Plains saw a significant amount of clinical disease in cattle. Phylogenetic analyses and sequence comparisons of newly sequenced whole genomes of 2012 EHDV-2 cattle isolates demonstrated that eight of ten EHDV-2 genomic segments show no genetic changes that separate the cattle outbreak sequences from other EHDV-2 isolates. Two segments, VP2 and VP6, did show several unique genetic changes specific to the 2012 cattle outbreak isolates, although the impact of the genetic changes on viral fitness is unknown. The placement of isolates from 2007 and 2011 as sister group to the outbreak isolates, and the similarity between cattle and deer isolates, point to environmental variables as having a greater influence on the severity of the 2012 EHDV outbreak than viral genetic changes.

10.1099/jgv.0.001221

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Citations (5)

Whole genome sequence analysis of circulating Bluetongue virus serotype 11 strains from the United States including two domestic canine isolates

Journal of Veterinary Diagnostic Investigation (2015)

Natasha N. Gaudreault Dane C. Jasperson Edward J. Dubovi Donna J. Johnson Eileen N. Ostlund

Bluetongue virus (BTV) is a vector-transmitted pathogen that typically infects and causes disease in domestic and wild ruminants. BTV is also known to infect domestic canines as discovered when dogs were vaccinated with a BTV-contaminated vaccine. Canine BTV infections have been documented through serological surveys, and natural infection by the Culicoides vector has been suggested. The report of isolation of BTV serotype 11 (BTV-11) from 2 separate domestic canine abortion cases in the states of Texas in 2011 and Kansas in 2012, were apparently unrelated to BTV-contaminated vaccination or consumption of BTV-contaminated raw meat as had been previously speculated. To elucidate the origin and relationship of these 2 domestic canine BTV-11 isolates, whole genome sequencing was performed. Six additional BTV-11 field isolates from Texas, Florida, and Washington, submitted for diagnostic investigation during 2011 and 2013, were also fully sequenced and analyzed. The phylogenetic analysis indicates that the BTV-11 domestic canine isolates are virtually identical, and both share high identity with 2 BTV-11 isolates identified from white-tailed deer in Texas in 2011. The results of the current study further support the hypothesis that a BTV-11 strain circulating in the Midwestern states could have been transmitted to the dogs by the infected Culicoides vector. Our study also expands the short list of available BTV-11 sequences, which may aid BTV surveillance and epidemiology.

Culicoides

10.1177/1040638715585156

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Citations (12)