Agnieszka Dettlaff‐Pokora

Gdańsk Medical University

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JŚ

Julian Świerczyński

Nicolaus Copernicus University

Wojciech Pokora

University of Gdańsk

Anna Aksmann

University of Gdańsk

Zbigniew Tukaj

University of Gdańsk

Agnieszka Baścik‐Remisiewicz

University of Gdańsk

TŚ

Tomasz Śledziński

Gdańsk Medical University

Beata Schlichtholz

Gdańsk Medical University

ŁH

Łukasz Hak

Gdańsk Medical University

Zbigniew Kmieć

Gdańsk Medical University

Elżbieta Goyke

Gdańsk Medical University

Cooperative Institutions

Gdańsk Medical University

University of Gdańsk

State Higher Vocational School in Koszalin

University Clinical Centre

Wojskowy Instytut Medyczny

Stomatology Hospital

Gdansk University of Physical Education and Sport

Nicolaus Copernicus University

Polish Academy of Sciences

Weatherford College

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Photosynthetic activity of Chlamydomonas reinhardtii wild type and CC-2699 mutant under Cd-stress

Acta Biologica Cracoviensia. Series Zoologia (2014)

Anna Aksmann Agnieszka Baścik‐Remisiewicz Wojciech Pokora Agnieszka Dettlaff‐Pokora Zbigniew Tukaj

Chlamydomonas reinhardtii

Wild type

Chlamydomonas

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High hydrogen peroxide production and antioxidative enzymes expression in the Chlamydomonas reinhardtii cia3 mutant with an increased tolerance to cadmium and anthracene

Phycological Research (2016)

Anna Aksmann Wojciech Pokora Agnieszka Baścik‐Remisiewicz Agnieszka Dettlaff‐Pokora Zbigniew Tukaj

SUMMARY Chlamydomonas reinhardtii cia3 mutant is deficient in activity of CAH 3, a carbonic anhydrase isoform necessary for proper functioning of photosystem II (PS II). We used cia3 to verify the hypothesis that despite high concentration of external CO 2 , deficiency in CAH 3 activity results in slight disorder of photosynthesis, which leads to mild but permanent overproduction of reactive oxygen species ( ROS ). As a consequence, cia3 should possess high ability to rebalance ROS homeostasis to overcome the oxidative stress induced by environmental factors, including chemical contaminations. We have found that in control conditions cia3 had a larger fraction of active PS II reaction centers but their efficiency was lower as compared with the wild type ( WT ), while H 2 O 2 production by cia3 was significantly higher than in WT . In regard to ROS ‐scavenging enzymes, the transcript level for superoxide dismutase ( SOD ) isoforms and the isoenzymes activity in cia3 were higher than in WT . There were no significant differences in the transcript level of ascorbate peroxidase ( APX ) and catalase ( CAT ) genes in both strains, and slightly elevated activity of the enzymes was observed in the cia3 ‐cells. Comparing the sensitivity of WT and cia3 to chemically‐induced stress we have found that the growth, photosynthesis and H 2 O 2 production in WT were more strongly affected by cadmium and anthracene as compared with cia3 . SOD s expression and activity in the treated WT were higher than those in cia3 , but APX and CAT activities were similar in both strains. Our results indicate that the CAH 3‐deficiency is associated with markedly elevated H 2 O 2 production and with a relatively higher initial transcript level and the activity of ROS ‐scavenging enzymes. In consequence, cia3 revealed a higher tolerance to cadmium and anthracene compared with WT .

Chlamydomonas reinhardtii

APX

Wild type

10.1111/pre.12147

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Citations (17)

Successful ageing of nonagenarians is related to the sensitivity of NK cells to activation.

PubMed (2008)

Lucyna Kaszubowska Agnieszka Dettlaff‐Pokora Łukasz Hak Magdalena Szaryńska Monika Ryba‐Stanisławowska

NK cells are a component of innate immunity which activity significantly correlates with health status. The aim of our study was to estimate a status of NK (natural killer) cells in the very old (mean age 92+/-2 ys) and old subjects (mean age 78+/-5 ys) as compared to a control group of young individuals (mean age 25+/-4 ys). NK cells were characterized by measurement of their cytotoxic activity, expression of intracellular interferon gamma, telomere length and telomerase activity in resting and activated cells. The results revealed that the oldest seniors did not differ from the other age groups in the number of NK cells and NK cytotoxic activity, however, they displayed the shortest telomeres and the lowest telomerase activity. Surprisingly, activated NK cells of the very old, similarly to the old subjects, were able to significantly increase intracellular level of IFNgamma. Moreover, activated with IL-2 NK cells of the old and oldest seniors showed increased telomerase activity. The results of our study suggest that the functional status of NK cells and their sensitivity to activation is well preserved until very advanced age and may contribute to longevity and successful ageing.

Immunosenescence

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Signal transducer and activator of transcription 3β in pancreatic cancer

Open Life Sciences (2010)

Agnieszka Dettlaff‐Pokora Justyna Kostro

Abstract Signal transducer and activator of transcription 3 (STAT3) has four isoforms: α, β, γ and δ. STAT3α and STAT3β are the transcriptionally active isoforms, while STAT3γ and STAT3δ are the products of STAT3 proteolytic degradation. STAT3 plays an important role in angiogenesis, cell proliferation and apoptosis. High levels of STAT3β in blood cells from acute leukaemia patients have been reported, suggesting that STAT3β may play an important role in cancerogenesis. Fourteen pancreatic cancer patients and six chronic pancreatitis patients were included in this pilot study. Levels of STAT3 isoforms from samples with pancreatic cancer and in adjacent histologically-normal pancreatic tissue were analysed. Pancreas from chronic pancreatitis patients served as a non-neoplastic tissue. Western-blot analysis of STAT3 proteins with the use of anti-STAT3 antibodies was performed. STAT3α and STAT3β isoforms in both cancerous and in adjacent normal tissues were found in 10 of 14. In chronic pancreatitis patients, only STAT3α and STAT3δ were detected. STAT3β was absent in pancreas from chronic pancreatitis patients, in contrast to pancreatic cancer patients. The presence of STAT3β in pancreatic cancer and in adjacent histologically-normal tissues, but not in inflamed tissues suggests that STAT3β may play a key role in cancer development.

10.2478/s11535-010-0029-x

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Citations (1)

Up-regulation of orexigenic and down-regulation of anorexigenic neuropeptide gene expression in rat hypothalamus after partial lipectomy

Journal of Applied Biomedicine (2015)

Agnieszka Dettlaff‐Pokora Tomasz Śledziński Julian Świerczyński

Leptin down-regulates orexigenic and up-regulates anorexigenic neuropeptide gene expression in hypothalamus. Surgical removal of adipose tissue leads to decrease in circulating leptin concentrations in rats. In the present study, we tested: (a) regulation of neuropeptide gene expression in hypothalamus, (b) food intake, and (c) standard growth rate after removal of adipose tissue in rats. Partial lipectomy caused an approximately 10-fold reduction of subcutaneous, retroperitoneal and epididymal adipose tissue weight (at the end of experiments adipose tissue weight was 1.5 ± 0.9 in lipectomy and 15 ± 3.9 g in control rats; statistically significant). Compared to control rats, the animals subjected to lipectomy presented increased food intake, standard growth rate, and decreased serum leptin concentrations (2.6 ± 0.8 vs. 3.7 ± 1.2 ng/mL in the controls, statistically significant). These changes were associated with approximately twofold increase in neuropeptide Y, threefold increase in agouti-related peptide (orexigenic neuropeptides) and about 50% decrease in pro-opiomelanocortin and cocaine-amphetamine-regulated transcript peptide (anorexigenic neuropeptides) mRNA levels in the hypothalamus. These results suggest that partial lipectomy, leading to a decrease in circulating leptin concentrations, may exert an effect on hypothalamic orexigenic and anorexigenic neuropeptide gene expression, and consequently modulate food intake and standard growth rate in rats.

Orexigenic

Proopiomelanocortin

10.1016/j.jab.2015.01.003

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Upregulation of Pnpla2 and Abhd5 and downregulation of G0s2 gene expression in mesenteric white adipose tissue as a potential reason for elevated concentration of circulating NEFA after removal of retroperitoneal, epididymal, and inguinal adipose tissue

Molecular and Cellular Biochemistry (2016)

Agnieszka Dettlaff‐Pokora Tomasz Śledziński Julian Świerczyński

Elevated concentrations of circulating non-esterified fatty acids (NEFA) were reported in (a) humans with lipodystrophy, (b) humans following bariatric surgery, and (c) transgenic mice with reduced amounts of adipose tissue. Paradoxically, these findings suggest that the reduction of adipose tissue mass is associated with elevated circulating NEFA concentrations. To explain a molecular background of this phenomenon, we analyzed the effects of surgical removal of inguinal, epididymal, and retroperitoneal white adipose tissue (WAT) on (a) circulating NEFA concentrations, (b) expression of Pnpla2, a gene that encodes adipose triglyceride lipase (ATGL), genes encoding abhydrolase domain containing 5 (ABHD5) and G0/G1 switch 2 (G0S2), i.e., a coactivator and inhibitor of ATGL, respectively, and (c) expression of Lipe gene coding hormone-sensitive lipase (HSL) in mesenteric WAT. Reduction of adipose tissue mass resulted in an increase in circulating NEFA concentration, which was associated with (a) an increase in the expressions of Pnpla2 and Abhd5, (b) decrease in G0s2 expression, and (c) upregulation of Lipe expression, all measured on both mRNA and protein levels in mesenteric WAT of male rats. The rate of lipolysis in mesenteric WAT explants and isolated adipocytes from lipectomized rats was significantly higher than that from the controls. In conclusion, upregulation of Pnpla2 expression and activation of ATGL (due to an increase in ABHD5 and decrease in G0S2 levels), as well as a coordinated interplay of these genes with Lipe in mesenteric WAT, contribute, at least in part, to an increase in the concentration of circulating NEFA in rats with reduced fat mass.

NEFA

Adipose triglyceride lipase

Hormone-sensitive lipase

10.1007/s11010-016-2800-4

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Citations (7)

Sensitivity of natural killer cells to activation in the process of ageing is related to the oxidative and inflammatory status of the elderly.

PubMed (2011)

Lucyna Kaszubowska Jan Jacek Kaczor Łukasz Hak Agnieszka Dettlaff‐Pokora Magdalena Szaryńska

The role of the innate immunity during human ageing is not well understood. The aim of the study was to estimate reactivity of NK (natural killer) cells in the very old (mean age 91 years) and old subjects (mean age 78 years) compared to young individuals (mean age 26 years) in respect to the indices of the oxidative stress (telomere length of NK cells, serum content of -H groups), serum total antioxidant status and serum concentrations of interleukin 6 and tumor necrosis factor-α (TNF-α). The activation state of NK cells, reflected by telomerase activity and intracellular interferon γ (IFNγ) content, was also measured. We found that length of telomeres in NK cells and serum concentration of -SH groups decreased both in the old and the oldest subjects as compared to young individuals. The oldest seniors, on the contrary to the old ones, revealed similar level of serum antioxidant status as the young subjects. The serum level of IL-6, not detectable in the young subjects, did not differ in the oldest and old seniors. TNF-α serum concentrations progressively increased with age. After stimulation, NK cells of both old groups showed higher intracellular levels of IFNγ than young subjects. IL-2-activated NK cells of the oldest seniors showed the highest increase of telomerase activity as compared to the other age groups. Serum level of IL-6 correlated positively with activation markers of NK cells. Moreover, in seniors but not in young subjects, the number of active, IFNγ-expressing NK cells, correlated positively with the serum content of the -SH groups. These findings indicate that sensitivity of NK cells to activation is maintained during ageing and this phenomenon may be related to the oxidative and inflammatory status of the elderly.

Immunosenescence

Senescence

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Citations (20)

Genetic basis of dyslipidemia

Agnieszka Dettlaff‐Pokora Julian Świerczyński Marek Szołkiewicz Leszek Rutkowski

Genetic disorders play an important role in the ethiopathogenesis of disease processes in humans. In the present paper we have described documented genes polymorphisms associated with dyslipidemia. Some of them have been known for years, but some have been discovered quite recently. Their characteristics an impact on lipid profile are presented. Discussed are their direct relationship with atherosclerosis and cardiovascular diseases.

Dyslipidemia

Lipid Profile

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Citations (1)

Exogenously applied hydrogen peroxide modifies the course of the Chlamydomonas reinhardtii cell cycle

Journal of Plant Physiology (2018)

Wojciech Pokora Anna Aksmann Agnieszka Baścik‐Remisiewicz Agnieszka Dettlaff‐Pokora Zbigniew Tukaj

Chlamydomonas reinhardtii

Chlamydomonas

Plant cell

10.1016/j.jplph.2018.07.015