Hidehiro Fukaki

Kobe University

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Masao Tasaka

Nara Institute of Science and Technology

Tetsuro Mimura

Kobe University

Tatsuaki Goh

Nara Institute of Science and Technology

Kimitsune Ishizaki

Kobe University

Miwa Ohnishi

Kobe University

Koichi Toyokura

Hiroshima University

Malcolm J. Bennett

University of Nottingham

Laurent Laplaze

Institut de Recherche pour le Développement

Yuki Kondo

Kobe University

Takayuki Kohchi

Kyoto University

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The University of Tokyo

Kyoto University

Kobe University

Nara Institute of Science and Technology

University of Nottingham

Japan Science and Technology Agency

Ghent University

National Institute for Basic Biology

Nagoya University

Osaka University

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Auxin Control of Root Development

Cold Spring Harbor Perspectives in Biology (2010)

Paul Overvoorde Hidehiro Fukaki Tom Beeckman

Paul Overvoorde1, Hidehiro Fukaki2 and Tom Beeckman3,4 Department of Biology, Macalester College, St. Paul, Minnesota 55105 Department of Biology, Kobe University, Rokkodai 1-1, 657-8501, Kobe, Japan Department of Plant Systems Biology, Flanders Institute for Biotechnology (VIB), Technologiepark 927, B-9052 Ghent, Belgium Department of Plant Biotechnology and Genetics, Ghent University, B-9052 Ghent, Belgium Correspondence: tom.beeckman{at}psb.ugent.be

Plant Roots

Root (linguistics)

Plant hormone

Lateral root

10.1101/cshperspect.a001537

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Citations (756)

SGR1, SGR2, and SGR3: Novel Genetic Loci Involved in Shoot Gravitropism in Arabidopsis thaliana

PLANT PHYSIOLOGY (1996)

Hidehiro Fukaki H. Fujisawa Masao Tasaka

In higher plants shoots show a negative gravitropic response but little is known about its mechanism. To elucidate this phenomenon, we have isolated a number of mutants with abnormal shoot gravitropic responses in Arabidopsis thaliana. Here we describe mainly three mutants: sgr1–1, sgr2–1, and sgr3–1 (shoot gravitropism). Genetic analysis confirmed that these mutations were recessive and occurred at three independent loci, named SGR1, SGR2, and SGR3, respectively. In wild type, both inflorescence stems and hypocotyls show negative gravitropic responses. The sgr1–1 mutants showed no response to gravity either by inflorescence stems or by hypocotyls. The sgr2–1 mutants also showed no gravitropic response in inflorescence stems but showed a reduced gravitropic response in hypocotyls. In contrast, the sgr3–1 mutant was found to have reduced gravitropic responses in inflorescence stems but normal gravitropic responses in hypocotyls. These results suggest that some genetic components of the regulatory mechanisms for gravitropic responses are common between inflorescence stems and hypocotyls, but others are not. In addition, these sgr mutants were normal with respect to root gravitropism, and their inflorescence stems and hypocotyls could carry out phototropism. We conclude that SGR1, SGR2, and SGR3 are novel genetic loci specifically involved in the regulatory mechanisms of shoot gravitropism in A. thaliana.

Gravitropism

Phototropism

10.1104/pp.110.3.945

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Citations (128)

The SHORT-ROOT Gene Controls Radial Patterning of the Arabidopsis Root through Radial Signaling

Cell (2000)

Ykä Helariutta Hidehiro Fukaki Joanna Wysocka‐Diller Keiji Nakajima Jee Jung

Root (linguistics)

10.1016/s0092-8674(00)80865-x

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Citations (1,074)

SGR2 (SHOOT GRAVITROPISM 2) gene relates to the embryogenesis in Arabidopsis thaliana

Plant and Cell Physiology (1998)

T. KATO Hidehiro Fukaki Masao Tasaka

Gravitropism

Source

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Citations (0)

Peptide-Receptor Signaling Controls Lateral Root Development

PLANT PHYSIOLOGY (2019)

Joris Jourquin Hidehiro Fukaki Tom Beeckman

Lateral root development progresses through different steps with, the peptides and receptors involved in each of these steps triggering downstream mechanisms upon peptide perception.

Lateral root

10.1104/pp.19.01317

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Citations (26)

Gravity perception and gravitropic response of inflorescence stems in Arabidopsis thaliana

Advances in Space Research (1999)

Hidehiro Fukaki Masao Tasaka

Gravitropism

Endodermis

10.1016/s0273-1177(99)00410-x

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Citations (29)

Reactive oxygen species and reactive carbonyl species constitute a feed‐forward loop in auxin signaling for lateral root formation

The Plant Journal (2019)

Md. Sanaullah Biswas Hidehiro Fukaki Izumi C. Mori Kazuha Nakahara Jun’ichi Mano

Summary In auxin‐stimulated roots, production of reactive oxygen species ( ROS ) via the hormone‐induced activation of respiratory burst oxidase homologous NADPH oxidases facilitates lateral root ( LR ) formation. In this study, in order to verify that ROS can modulate auxin signaling, we examined the involvement of the lipid peroxide‐derived agents known as reactive carbonyl species ( RCS ) in LR formation. When auxin was added to Arabidopsis thaliana roots, the levels of RCS , for example acrolein, 4‐hydroxynonenal and crotonaldehyde, were increased prior to LR formation. Addition of the carbonyl scavenger carnosine suppressed auxin‐induced LR formation. Addition of RCS to the roots induced the expression of the auxin‐responsive DR 5 promoter and the TIR 1 , IAA 14 , ARF 7 , LBD 16 and PUCHI genes and facilitated LR formation without increasing the endogenous auxin level. DR 5 and LBD 16 were activated in the LR primordia. The auxin signaling‐deficient mutants arf7 arf19 and slr‐1 did not respond – and tir1 afb2 appeared to show a poor response – to RCS . When given to the roots RCS promoted the disappearance of the AXR 3 NT – GUS fusion protein, i.e. the degradation of the auxin/indole‐3‐acetic acid protein, as did auxin. These results indicate that the auxin‐induced production of ROS and their downstream products RCS modulate the auxin signaling pathway in a feed‐forward manner. RCS are key agents that connect the ROS signaling and the auxin signaling pathways.

10.1111/tpj.14456

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Citations (63)

Ion gradients in xylem exudate and guttation fluid related to tissue ion levels along primary leaves of barley

Plant Cell & Environment (2013)

Makiko Nagai Miwa Ohnishi Takeo Uehara Mutsumi Yamagami Eiko Miura

The concentration of ions in plant cells and tissues is an essential factor in determining physiological function. In the present study, we established that concentration gradients of mobile ions exist in both xylem exudates and tissues within a barley (Hordeum vulgare) primary leaf. For K(+) and NO3 (-) , ion concentrations generally decreased from the leaf base to the tip in both xylem exudates and tissues. Ion gradients were also found for Pi and Cl(-) in the xylem. The hydathode strongly absorbed Pi and re-translocated it to the rest of the plant, whereas Cl(-) was extruded. The ion concentration gradients developed early during leaf growth, increased as the tissue aged and remained under both high and low transpiration conditions. Measurement of the expression profiles of Pi, K(+) and NO3 (-) transporters along the longitudinal axis of the leaf revealed that some transporters are more expressed at the hydathode, but for most transporters, there was no significant variation along the leaf. The mechanisms by which longitudinal ion gradients develop in leaves and their physiological functions are discussed.

Exudate

Transpiration stream

Concentration gradient

10.1111/pce.12090

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Citations (42)

Regulation of ROP GTPase cycling between active/inactive states is essential for vegetative organogenesis in Marchantia polymorpha

Development (2024)

Yuuki Sakai Aki Ueno Hiroki Yonetsuka Tatsuaki Goh Hirotaka Kato

ABSTRACT Rho/Rac of plant (ROP) GTPases are plant-specific proteins that function as molecular switches, activated by guanine nucleotide exchange factors (GEFs) and inactivated by GTPase-activating proteins (GAPs). The bryophyte Marchantia polymorpha contains single copies of ROP (MpROP), GEFs [ROPGEF and SPIKE (SPK)] and GAPs [ROPGAP and ROP ENHANCER (REN)]. MpROP regulates the development of various tissues and organs, such as rhizoids, gemmae and air chambers. The ROPGEF KARAPPO (MpKAR) is essential for gemma initiation, but the functions of other ROP regulatory factors are less understood. This study focused on two GAPs: MpROPGAP and MpREN. Mpren single mutants showed defects in thallus growth, rhizoid tip growth, gemma development, and air-chamber formation, whereas Mpropgap mutants showed no visible abnormalities. However, Mpropgap Mpren double mutants had more severe phenotypes than the Mpren single mutants, suggesting backup roles of MpROPGAP in processes involving MpREN. Overexpression of MpROPGAP and MpREN resulted in similar gametophyte defects, highlighting the importance of MpROP activation/inactivation cycling (or balancing). Thus, MpREN predominantly, and MpROPGAP as a backup, regulate gametophyte development, likely by controlling MpROP activation in M. polymorpha.

Organogenesis

Marchantia polymorpha

10.1242/dev.202928

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Citations (1)

RLF, a cytochrome b5-like heme/steroid binding domain protein, controls lateral root formation independently of ARF7/19-mediated auxin signaling in Arabidopsis thaliana

The Plant Journal (2010)

Yoshifumi Ikeyama Masao Tasaka Hidehiro Fukaki

Lateral root (LR) formation is important for the establishment of root architecture in higher plants. Recent studies have revealed that LR formation is regulated by an auxin signaling pathway that depends on auxin response factors ARF7 and ARF19, and auxin/indole-3-acetic acid (Aux/IAA) proteins including SOLITARY-ROOT (SLR)/IAA14. To understand the molecular mechanisms of LR formation, we isolated a recessive mutant rlf (reduced lateral root formation) in Arabidopsis thaliana. The rlf-1 mutant showed reduction of not only emerged LRs but also LR primordia. Analyses using cell-cycle markers indicated that the rlf-1 mutation inhibits the first pericycle cell divisions involved in LR initiation. The rlf-1 mutation did not affect auxin-induced root growth inhibition but did affect LR formation over a wide range of auxin concentrations. However, the rlf-1 mutation had almost no effect on auxin-inducible expression of LATERAL ORGAN BOUNDARIES-DOMAIN16/ASYMMETRIC LEAVES2-LIKE18 (LBD16/ASL18) and LBD29/ASL16 genes, which are downstream targets of ARF7/19 for LR formation. These results indicate that ARF7/19-mediated auxin signaling is not blocked by the rlf-1 mutation. We found that the RLF gene encodes At5g09680, a protein with a cytochrome b5-like heme/steroid binding domain. RLF is ubiquitously expressed in almost all organs, and the protein localizes in the cytosol. These results, together with analysis of the genetic interaction between the rlf-1 and arf7/19 mutations, indicate that RLF is a cytosolic protein that positively controls the early cell divisions involved in LR initiation, independent of ARF7/19-mediated auxin signaling.

Lateral root

Pericycle

Primordium

10.1111/j.1365-313x.2010.04199.x

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Citations (28)