Guilherme Henrique Tamarindo

Universidade Estadual de Campinas (UNICAMP)

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Rejane M. Góes

Universidade Estadual Paulista (Unesp)

Sebastião Roberto Taboga

Universidade Estadual Paulista (Unesp)

Adriana Alonso Novais

Universidade Federal de Mato Grosso

Débora Aparecida Pires de Campos Zuccari

Faculdade de Medicina de São José do Rio Preto

Luiz Gustavo de Almeida Chuffa

Universidade Estadual Paulista (Unesp)

Juliana S. Maldarine

Universidade Estadual de Campinas (UNICAMP)

Bruno D. A. Sanches

Universidade Estadual de Campinas (UNICAMP)

Marina Guimarães Gobbo

Faculdade de Medicina de São José do Rio Preto

Hernandes F. Carvalho

Universidade Estadual de Campinas (UNICAMP)

Paula Rahal

Universidade Estadual Paulista (Unesp)

Cooperative Institutions

Universidade Estadual Paulista (Unesp)

Universidade Estadual de Campinas (UNICAMP)

Faculdade de Medicina de São José do Rio Preto

Universidade de São Paulo

Centro Universitário de Rio Preto

John Wiley & Sons (United States)

Hudson Institute

Cornell University

Weill Cornell Medicine

Harvard University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Decoding Hidden Messengers: Proteomic Profiling of Exosomes in Mammary Cancer Research

Biomedicines (2023)

Adriana Alonso Novais Guilherme Henrique Tamarindo Luiz Gustavo de Almeida Chuffa Débora Aparecida Pires de Campos Zuccari

Cancer is a complex and heterogeneous disease, influenced by various factors that affect its progression and response to treatment. Although a histopathological diagnosis is crucial for identifying and classifying cancer, it may not accurately predict the disease’s development and evolution in all cases. To address this limitation, liquid biopsy has emerged as a valuable tool, enabling a more precise and non-invasive analysis of cancer. Liquid biopsy can detect tumor DNA fragments, circulating tumor cells, and exosomes released by cancer cells into the bloodstream. Exosomes attracted significant attention in cancer research because of their specific protein composition, which can provide valuable insights into the disease. The protein profile of exosomes often differs from that of normal cells, reflecting the unique molecular characteristics of cancer. Analyzing these proteins can help identify cancer-associated markers that play important roles in tumor progression, invasion, and metastasis. Ongoing research and clinical validation are essential to advance and effectively utilize protein biomarkers in cancer. Nevertheless, their potential to improve diagnosis and treatment is highly promising. This review discusses several exosome proteins of interest in breast cancer, particularly focusing on studies conducted in mammary tissue and cell lines in humans and experimental animals. Unfortunately, studies conducted in canine species are scarce. This emphasis sheds light on the limited research available in this field. In addition, we present a curated selection of studies that explored exosomal proteins as potential biomarkers, aiming to achieve benefits in breast cancer diagnosis, prognosis, monitoring, and treatment.

Profiling (computer programming)

10.3390/biomedicines11102839

Cite

Citations (4)

Docosahexaenoic acid differentially modulates the cell cycle and metabolism- related genes in tumor and pre-malignant prostate cells

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids (2020)

Guilherme Henrique Tamarindo Rejane M. Góes

10.1016/j.bbalip.2020.158766

Cite

Citations (12)

Docosahexaenoic acid effects on cell metabolism in prostate cancer initiation and progression

Guilherme Henrique Tamarindo

Magnetic Resonance / Ressonância Magnética Nuclear de Prótons 22rv1 -Linhagem prostática epitelial humana tumoral andrógeno-responsiva 7-AAD -7-aminoactinomycin D / 7-aminoactinomicina D ACC -Acetyl-CoA carboxylase / Acetil-CoA carboxilase ACLY -ATP citrate lyase

ATP citrate lyase

Lyase

10.47749/t/unicamp.2022.1238622

Cite

Citations (0)

Telocytes of the male urogenital system: Interrelationships, possible functions, and pathological implications

Cell Biology International (2021)

Bruno D. A. Sanches Guilherme Henrique Tamarindo Juliana S. Maldarine Alana Della Torre da Silva Vitória A. Santos

The male urogenital system is composed of the reproductive system and the urinary tract; they have an interconnected embryonic development and share one of their anatomical components, the urethra. This system has a highly complex physiology deeply interconnected with the circulatory and nervous systems, as well as being capable of adapting to environmental variations; it also undergoes changes with aging and, in the case of the reproductive system, with seasonality. The stroma is an essential component in this physiological plasticity and its complexity has increased with the description in the last decade of a new cell type, the telocyte. Several studies have demonstrated the presence of telocytes in the organs of the male urogenital system and other systems; however, their exact function is not yet known. The present review addresses current knowledge about telocytes in the urogenital system in terms of their locations, interrelationships, possible functions and pathological implications. It has been found that telocytes in the urogenital system possibly have a leading role in stromal tissue organization/maintenance, in addition to participation in stem cell niches and an association with the immune system, as well as specific functions in the urogenital system, lipid synthesis in the testes, erythropoiesis in the kidneys and the micturition reflex in the bladder. There is also evidence that telocytes are involved in pathologies in the kidneys, urethra, bladder, prostate, and testes.

Reproductive system

10.1002/cbin.11612

Cite

Citations (23)

Telocytes contribute to aging-related modifications in the prostate

Scientific Reports (2020)

Bruno D. A. Sanches Guilherme Henrique Tamarindo Juliana S. Maldarine Alana Della Torre da Silva Vitória A. Santos

Abstract Telocytes are interstitial cells present in the stroma of several organs, including the prostate. There is evidence that these cells are present during prostate alveologenesis, in which these cells play a relevant role, but there is no information about the presence of and possible changes in telocytes during prostate aging. Throughout aging, the prostate undergoes several spontaneous changes in the stroma that are pro-pathogenic. Our study used histochemistry, 3D reconstructions, ultrastructure and immunofluorescence to compare the adult prostate with the senile prostate of the Mongolian gerbil, in order to investigate possible changes in telocytes with senescence and a possible role for these cells in the age-associated alterations. It was found that the layers of perialveolar smooth muscle become thinner as the prostatic alveoli become more dilated during aging, and that telocytes form a network that involves smooth muscle cells, which could possibly indicate a role for telocytes in maintaining the integrity of perialveolar smooth muscles. On the other hand, with senescence, VEGF+ telocytes are seen in stroma possibly contributing to angiogenesis, together with TNFR1+ telocytes, which are associated with a pro-inflammatory microenvironment in the prostate. Together, these data indicate that telocytes are important both in understanding the aging-related changes that are seen in the prostate and also in the search for new therapeutic targets for pathologies whose frequency increases with age.

10.1038/s41598-020-78532-7

Cite

Citations (19)

Do mineral and corn oil serve as potential endocrine disruptors in the gerbil prostate?

Reproductive Toxicology (2019)

Luiz Henrique Alves Guerra Guilherme Henrique Tamarindo Silvana G. P. Campos Sebastião Roberto Taboga Patrícia Simone Leite Vilamaior

Corn oil

Mineral oil

Gerbil

10.1016/j.reprotox.2019.09.004

Cite

Citations (7)

Pathological lesions and global DNA methylation in rat prostate under streptozotocin‐induced diabetes and melatonin supplementation

Cell Biology International (2017)

Marina Guimarães Gobbo Guilherme Henrique Tamarindo Daniele Lisboa Ribeiro Silvana G. P. Campos Sebastião Roberto Taboga

Chronic hyperglycemia increases production of reactive oxygen species, which favors carcinogenesis. The association between diabetes and prostate cancer is controversial. Melatonin has antioxidant, anti-inflammatory, and antiproliferative properties. We investigated whether low doses of melatonin prevent the tissue alterations caused by diabetes and alter prostate histology of healthy rats. We also investigated whether experimental diabetes promoted the development of pathological lesions in the ventral prostate of rats. Melatonin was provided in drinking water (10 μg/kg/day) from age 5 weeks until the end of experiment. Diabetes was induced at 13 weeks by administration of streptozotocin (40 mg/kg, ip). Rats were euthanized at 14 or 21 weeks. Histological and stereological analyses were carried out and the incidence and density of malignant and pre-malignant lesions were assessed. Immunohistochemical assays of α-actin, cell proliferation (PCNA), Bcl-2, glutathione S-transferase (GSTPI), and DNA methylation (5-methylcytidine) were performed. Melatonin did not elicit conspicuous changes in the prostate of healthy animals; in diabetic animals there was a higher incidence of atrophy (93%), microinvasive carcinoma (10%), proliferative inflammatory atrophy, PIA (13%), prostatitis (26%), and prostate intraepithelial neoplasia, PIN (20%) associated with an increase of 40% in global DNA methylation. Melatonin attenuated epithelial and smooth muscle cell (smc) atrophy, especially at short-term diabetes-and normalized incidence of PIN (11%), inflammatory cells infiltrates, prostatitis (0%) and PIA (0%) at long-term diabetes. MLT was effective in preventing inflammatory disorders and PIN under diabetic condition. Although MLT has antioxidant action, it did not influence DNA methylation and not avoid carcinogenesis at low doses.

10.1002/cbin.10920

Cite

Citations (7)

Differential effects of omega‐3 PUFAS on tumor progression at early and advanced stages in TRAMP mice

The Prostate (2022)

Gustavo M. Amaro Alana D. T. da Silva Guilherme Henrique Tamarindo Celina de Almeida Lamas Sebastião Roberto Taboga

Abstract Background In vitro studies evidenced antitumor effects of omega‐3 polyunsaturated fatty acids ([ n −3] PUFAs), but their effects on prostate cancer (PCa) remain controversial in epidemiological studies. Here we investigated whether an ( n −3) PUFA‐enriched diet affects tumor progression in transgenic adenocarcinoma of the mouse prostate (TRAMP), at early (12 weeks age) and advanced stages (20 weeks age). Methods TRAMP mice were fed with standard rodent diet (C12, C20) or ( n −3) PUFA‐enriched diet containing 10% fish oil (T12, T20). A group of 8 weeks age animals fed standard diet was also used for comparison (C8). The ventral prostate was processed for histopathological and immunohistochemical analyses and serum samples submitted to biochemical assays. Results At early stages, ( n −3) PUFA increased the frequency of normal epithelium (3.8‐fold) and decreased the frequency of high‐grade intraepithelial neoplasia (3.3‐fold) and in situ carcinoma (1.9‐fold) in the gland, maintaining prostate pathological status similar to C8 group. At advanced stages, 50% of the animals developed a large primary tumor in both C20 and T20, and tumor weight did not differ (C20: 2.2 ± 2.4; T20: 2.8 ± 2.9 g). The ventral prostate of T12 and of T20 animals that did not develop primary tumors showed lower cell proliferation, tissue expressions of androgen (AR) and glucocorticoid (GR) receptors, than their respective controls. For these animals, ( n −3) PUFA also avoided an increase in the number of T‐lymphocytes, collagen fibers, and αSMA immunoreactivity, and preserved stromal gland microenvironment. ( n −3) PUFA also lowered serum triglycerides and cholesterol, regulating the lipid metabolism of TRAMP mice. Conclusions ( n −3) PUFAs had a protective effect at early stages of PCa, delaying tumor progression in TRAMP mice, in parallel with reductions in cell proliferation, AR, and GR and maintenance of the stromal compartment of the gland. However, ( n −3) PUFAs did not prevent the development of primary tumors for the T20 group, reinforcing the need for further investigation at advanced stages of disease.

Tramp

Tumor progression

10.1002/pros.24421

Cite

Citations (3)

Unveiling the complexity: Liquid biopsy-based proteomic exploration of triple-negative breast cancer in women.

Journal of Clinical Oncology (2024)

Débora Aparecida Pires de Campos Zuccari Guilherme Henrique Tamarindo Adriana Alonso Novais Barbara Maria Frigieri Juliano Coelho da Silveira

e13138 Background: This groundbreaking study places a spotlight on breast cancer in women, with a specific emphasis on the challenging triple-negative breast cancer (TNBC) subtype. Delving into the intricate world of extracellular vesicles (EVs) from TNBC-diagnosed individuals, the research meticulously compares various conditions, including healthy controls, benign tumors, recurrent tumors, and those in remission. Employing cutting-edge techniques like liquid biopsy and LC-MS/MS, the study meticulously explores the proteomic profile of serum exosomes, aiming to pinpoint diagnostic and prognostic markers in breast cancer. Methods: In the initial phase, EVs were expertly isolated and characterized from blood plasma samples exclusively from women, utilizing state-of-the-art methods such as ultracentrifugation and nanoparticle tracking analysis (NTA). Despite applying rigorous statistical criteria, the study found no significant differences in EV concentrations and sizes among the diverse groups. However, the ensuing proteomic analysis uncovered 130 proteins, with Cystatin A and Histone H2A emerging as promising biomarkers for TNBC. To validate them, the study intricately tested tumor cell lines from distinct subtypes, representing luminal, triple-negative, and HER2 overexpressed. The examination of gene and protein expression through RT-qPCR and western blotting added a layer of depth to the research, unraveling the complexity of regulatory processes within cells and demonstrating a remarkable congruence between protein levels found in the proteomics from patients’ serum and in immortalized cell lines. Results: The proteomic analysis brought to light the absence of CSTA and HIST1H2A expression in benign tumor patients, varied levels in malignant tumor patients, and a substantial increase in TNBC patients pre-mastectomy. Validating these findings through western blotting further accentuated the potential of these proteins as distinguishing factors. Conclusions: In conclusion, this pioneering study not only underscores the immense potential of liquid biopsy-based proteomic analysis in identifying crucial diagnostic and prognostic markers in breast cancer but also sheds light on the intricate nuances of the challenging TNBC subtype. Our findings unveil valuable insights that pave the way for future personalized treatment strategies, marking a significant stride forward in breast cancer research.

Triple-negative breast cancer

Liquid biopsy

Triple negative

10.1200/jco.2024.42.16_suppl.e13138

Cite

Citations (0)

Toward an Improved Understanding and Treatment of Canine Mammary Tumors: Insights and Advances from the Research

Animals (2024)

Débora Aparecida Pires de Campos Zuccari Adriana Alonso Novais Guilherme Henrique Tamarindo Luiz Gustavo de Almeida Chuffa

As Guest Editors of this Special Issue on canine mammary tumors, we are pleased to present a collection of articles on this highly relevant and timely topic [...]

10.3390/ani14131890

Cite

Citations (0)