Marija Stevanov-Pavlović

University of Belgrade

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Zoran Stanimirović

University of Belgrade

Jevrosima Stevanović

University of Belgrade

Jasna Bošnjak

University of Belgrade

Miloš Vučićević

University of Belgrade

Zora Mijačević

University of Belgrade

Milica Kovačević Filipović

University of Belgrade

Vladimir Dimitrijević

University of Belgrade

Tatjana Božić

University of Belgrade

Radmila Resanović

University of Belgrade

Dejan Radović

University of Belgrade

Cooperative Institutions

University of Belgrade

Croatian Veterinary Institute

University of Veterinary Medicine

University of Belgrade – Faculty of Veterinary Medicine

Weatherford College

Military Medical Academy

Agricultural institute of Slovenia

Institut za kardiovaskularne bolesti Dedinje

Centre of Research Excellence in Nutrition and Metabolism

University of Belgrade – Faculty of Security Studies

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis

Veterinary Immunology and Immunopathology (2011)

Milica Kovačević Filipović Vesna Ilić Zoran Vujčić Biljana Dojnov Marija Stevanov-Pavlović

Subclinical infection

Somatic Cell Count

10.1016/j.vetimm.2011.10.015

Cite

Citations (26)

Acute phase protein response in cows with staphylococcus aureus subclinical mastitis

Acta veterinaria (2010)

Milica Kovačević Filipović Jelka Stevanović Marija Stevanov-Pavlović Jasmina Debeljak-Martačić Djordje Knežević

Inflammation at a local and systemic level is a complex process that involves the synthesis of acute phase proteins (APPs) with multiple functions in the regulation of the inflammatory process itself. The aim of this work was to define local and systemic APPs response induced by natural Staph. aureus subclinical infection of the mammary gland in dairy cows with a different number of quarters involved. Midlactation dairy cows (n=30) were devided into three groups. First group were cows with bacteriologically negative milk samples (BN group), second group were cows with one quarter infected with Staph. aureus (SaQ1) and third group were cows with two quarters infected (SaQ2). Milk samples were analyzed for inflammation indicators: serum amyloid A (SAA) and somatic cell count (SCC). Serum samples where analyzed for SAA, haptoglobin (Hp), ceruloplasmin (Cp) and albumin concentration. Also, complete blood count (CBC) was done. SCC and SAA increased in quarter milk samples, being lowest in the BN group and highest in the SaQ2 group. In serum samples, SAA, Hp, Cp and albumin concentrations were significantly higher only in the SaQ2 group comparing with BN group. The leukocyte number, as well as hemoglobin concentration were in the physiological range in all three groups of cows. These results confirm that the magnitude of tissue injury has an impact on APPs concentration. They also demonstrate that cows having Staph. aureus sublinical infections of two mammary quarters have a more pronounced systemic APP response than cows with only one quarter involved.

Haptoglobin

Somatic Cell Count

Subclinical infection

10.2298/avb1003205k

Cite

Citations (5)

Sex Determination in 58 Bird Species and Evaluation of CHD Gene as a Universal Molecular Marker in Bird Sexing

Zoo Biology (2012)

Miloš Vučićević Marija Stevanov-Pavlović Jevrosima Stevanović Jasna Bošnjak Bojan Gajić

The aim of this research was to test the CHD gene ( C hromo H elicase DNA ‐binding gene) as a universal molecular marker for sexing birds of relatively distant species. The CHD gene corresponds to the aim because of its high degree of conservation and different lengths in Z and W chromosomes due to different intron sizes. DNA was isolated from feathers and the amplification of the CHD gene was performed with the following sets of polymerase chain reaction ( PCR ) primers: 2550 F /2718 R and P2/ P 8. Sex determination was attempted in 284 samples of 58 bird species. It was successful in 50 bird species; in 16 of those ( Alopochen aegyptiacus , Ara severus , Aratinga acuticaudata , Bucorvus leadbeateri , Cereopsis novaehollandiae , Columba arquatrix , Corvus corax , C. frugilegus , Cyanoliseus patagonus , Guttera plumifera , Lamprotornis superbus , Milvus milvus , Neophron percnopterus , Ocyphaps lophotes , Podiceps cristatus, and Poicephalus senegalus ), it was carried out for the first time using molecular markers and PCR . It is reasonable to assume that extensive research is necessary to define the CHD gene as a universal molecular marker for successful sex determination in all bird species (with exception of ratites). The results of this study may largely contribute to the aim. Zoo Biol 32:269–276, 2013. © 2012 Wiley Periodicals, Inc.

Sexing

10.1002/zoo.21010

Cite

Citations (63)

APPLICABILITY ASSESSMENT Of A STANdARdIZEd MICROSATELLITE MARKER SET IN ENdANgEREd BuSHA CATTLE

Slovenian Veterinary Research (2015)

Marija Stevanov-Pavlović Vladimir Dimitrijević Saša Marić Dejan Radović Jevrosima Stevanović

Summary: This study is focused on evaluating 12 microsatellite markers (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, BM1818, ETH3, ETH225, BM1824) as recommended by International Society of Animal Genetics (ISAG) for paternity testing in Busha cattle in Serbia. A herd of 47 heads of Busha cattle were sampled for blood. The number of alleles ranged from 6 (ETH10) to 16 (TGLA122), with a mean value of 9.5. Total number of alleles at all 12 analyzed loci was 114. PIC (Polymorphism Information Content) values ranged from 0.513 (in BM1818) to 0.905 (TGLA53). Power of exclusion (PE) for single markers ranged from 0.228 (BM1818) to 0.607 (BM2113) and the power of discrimination (PD) from 0.75 (BM1818) to 0.96 (TGLA227). The combined power of exclusion and discrimination was very high (0.999 and 0.995 respectively) when all 12 markers were used in combination. Cervus software performed 96% successful paternity assignments. These results show that the 12 markers set recommended by ISAG can be used with high confidence for forensic purposes in Busha cattle.

Source

Cite

Citations (3)

Molecular sex determination of 20 bird species protected in the Republic of Serbia

Acta veterinaria (2013)

Marija Stevanov-Pavlović Miloš Vučićević Jasna Bošnjak Jevrosima Stevanović Vladimir Dimitrijević

Considering that more than 50% of bird species are monomorphic, the identification of gender based on phenotypic characteristics is extremely difficult. The aim of this study is sex determination in species inhabiting the Republic of Serbia, all under the state protection and declared by IUCN as endangered. DNA was isolated from feathers. Sex determination was based on sex-specific CHD gene amplified by 2550F/2718R primer set. Sexing gave good results in all 91 samples from 20 species including 6 species where molecular sexing has not previously been successful.

Sexing

Primer (cosmetics)

10.2298/avb1301045s

Cite

Citations (12)

Feasibility of non-invasive molecular method for sexing of parrots.

Pakistan Journal of Zoology (2013)

Jasna Bošnjak Marija Stevanov-Pavlović Miloš Vučićević Jevrosima Stevanović Predrag Simeunović

The aim of this study was to test the feasibility and efficacy of a non-invasive molecular method for gender identification of parrots, using different types of samples and the Chromo Helicase DNA-binding (CHD) gene as a molecular marker. DNA was isolated primarily from feathers and the amplification of the CHD gene was performed using 2550F/2718R primers. In order to compare the reliability of different sources of DNA, we used buccal swab, blood and feces. All sample types exerted successful sexing results with the exception of feces samples where the success rate was 25%. Sexing was successfully determined in 239 birds belonging to 32 species of parrots. In 6 species (Amazona finschi, A. leucocephala, Aratinga aurea, Barnardius zonarius, Coracopsis nigra and Nymphicus hollandicus), 2550F/2718R primers proved to work well for the first time. Species used in this study are on the IUCN red list of threatened species. Furthermore, Amazona finschi, A. leucocephala, Cacatua moluccensis and C. sulphurea are on the list of CITES Apendix I. Since the failure in reproduction is one of the main causes of illegal trafficking of parrots, the non-invasive and universal molecular sexing method we tested may be a very useful tool in the preservation of endangered parrot species.

Sexing

Source

Cite

Citations (23)