Shaohui Lin

Shanghai Ninth People's Hospital

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i-10 index

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Shaojun Ma

Shanghai Ninth People's Hospital

Chunyan Wang

Chinese PLA General Hospital

Wang Hai-ya

Shanghai Ninth People's Hospital

Li Wang

George Mason University

Peng Yin

Capital Medical University

Qiang Huang

Soochow University

Jiajun Wang

China Medical University

Wenwei Cai

Zhejiang Provincial People's Hospital

Dongjuan Yu

Yongbo Hu

Changhai Hospital

Cooperative Institutions

Shanghai Jiao Tong University

Ruijin Hospital

Shanghai Ninth People's Hospital

Chinese Academy of Sciences

Fudan University

Capital Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

National Center for Chronic and Noncommunicable Disease Control and Prevention

Chinese Center For Disease Control and Prevention

Lanzhou University

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Different Gastrointestinal Microbiota Related to Bile-Acid Metabolism in Hypertensive Patients With or Without Nephropathy

Research Square (Research Square) (2021)

Xin Li Li Wang Shaojun Ma Shaohui Lin Chunyan Wang

Abstract Background: The human microbiome is a mixed group of microorganisms and has been verified the relationship between gastrointestinal microbiota and blood pressure. The intestinal microbiota of chronic kidney disease (CKD) patients in the distribution of bacterial species is different from the flora of people with no CKD. The purpose of this research is to study on the different intestinal microbiota of hypertensive patients with and without nephropathy and to find possible bio-markers to diagnose hypertensive nephropathy, as well as one of the basis for hypertensive nephropathy or CKD clinical intervention treatment. Methods: The study subjects were divided into three groups, normal control, simple hypertension and hypertensive nephropathy. Sequencing, bioinformatics and statistical analysis were performed on the 16S rRNA gene of the flora of the subjects' stool samples. Results: This research shows the difference of intestinal flora in hypertension patients with and without nephropathy; it investigates the relationship of the differences in the intestinal microbiota with bile-acid metabolism; it also explores bile-acid metabolism mechanism of intestinal microbiota differences of hypertensive with or without nephropathy. Conclusions: The different gastrointestinal microbiota is related to bile-acid metabolism in hypertensive patients, and can be one of the factors causing CKD. Trial registration: This article does not report the results of a health care intervention on human participants.

Flora

Hypertensive Nephropathy

10.21203/rs.3.rs-142746/v1

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Citations (1)

Combination of Oxalobacter Formigenes and Veillonella Parvula in Gastrointestinal Microbiota Related to Bile-Acid Metabolism as a Biomarker for Hypertensive Nephropathy

International Journal of Hypertension (2022)

Xin Li Li Wang Shaojun Ma Shaohui Lin Chunyan Wang

The human microbiome is a mixed group of microorganisms, which individually consists of 10-100 trillion symbiotic microbial cells. The relationship between gastrointestinal microbiota and blood pressure has been verified and the intestinal microbiota of chronic kidney disease (CKD) patients in the distribution of bacterial species is different from the flora of people with no CKD. The purpose of this research is to study the different intestinal microbiota of hypertensive patients with and without nephropathy and to find possible biomarkers of hypertensive nephropathy (H-CKD). The subjects of this research were divided into three groups, healthy control group, hypertension group, and hypertensive nephropathy group. Sequencing, bioinformatics, and statistical analysis were performed on the 16S rRNA gene of the subjects' stool samples. This research study showed the differences of intestinal flora as biomarkers in hypertension patients with and without nephropathy; it investigated the relationship of the differences in the intestinal microbiota with bile-acid metabolism; it also explored bile-acid metabolism mechanism of intestinal microbiota differences in hypertension with or without nephropathy. In summary, the difference in the combination of O. formigenes and V. parvula in the gastrointestinal microbiota is related to bile-acid metabolism in hypertensive patients and can be one of the factors causing CKD. It is the first time to report such a biomarker or pathogenic factor of H-CKD in the world.

Veillonella

10.1155/2022/5999530

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The China Alzheimer Report 2022

General Psychiatry (2022)

Ru‐Jing Ren Jinlei Qi Shaohui Lin Xinya Liu Peng Yin

China's population has rapidly aged over the recent decades of social and economic development as neurodegenerative disorders have proliferated, especially Alzheimer's disease (AD) and related dementias (ADRD). AD's incidence rate, morbidity, and mortality have steadily increased to make it presently the fifth leading cause of death among urban and rural residents in China and magnify the resulting financial burdens on individuals, families and society. The 'Healthy China Action' plan of 2019-2030 promotes the transition from disease treatment to health maintenance for this expanding population with ADRD. This report describes related epidemiological trends, evaluates the economic burden of the disease, outlines current clinical diagnosis and treatment status and delineates existing available public health resources. More specifically, it examines the public health impact of ADRD, including prevalence, mortality, costs, usage of care, and the overall effect on caregivers and society. In addition, this special report presents technical guidance and supports for the prevention and treatment of AD, provides expertise to guide relevant governmental healthcare policy development and suggests an information platform for international exchange and cooperation.

Epidemiological transition

Population Ageing

Action plan

10.1136/gpsych-2022-100751

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Citations (206)

Fatty acid profiling of blood cell membranes by gas chromatography with mass spectrometry

Journal of Separation Science (2016)

Zequn Liu Shaohui Lin Rui Weng Yali Liu Tianyu Li

Fatty acids, which are well-known for their influence on human metabolism and signal transduction, are also a substantial component of cellular membranes and regulate the basic properties and functions of membranes. Owing to their multiple functions, fatty acid profiles of cell membranes are of great interest to those who are studying the relationship between membrane biochemical compositions and functions. A HCl-catalyzed derivation method and a gas chromatography with mass spectrometry analysis method were developed to accurately profile the fatty acids in cell membranes of erythrocytes, leukocytes, and platelets. The detection limits of all 35 fatty acids ranged from 0.58 to 22 ng/mL and the limits of quantitation were between 2.1 and 72 ng/mL. Finally, the established method was used to profile the membrane fatty acids of 44 healthy volunteers from the north and south of China. Results revealed significant differences in the fatty acid profiles from the two regions, particularly those of the erythrocytes. This technique may be applied to cell membrane studies to generate new biological hypotheses concerning fatty acid composition and membrane functions as well as to construct related disease profiles.

Cell membrane

Fatty Acid Metabolism

10.1002/jssc.201600461

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Effect of CTRP3 on activation of adventitial fibroblasts induced by TGF-β1 from rat aorta in vitro.

PubMed (2014)

Shaohui Lin Shaojun Ma Ping Lu Wenwei Cai Yi Chen

CTRP3, discovered as novel adipokines, is a member of the C1q tumor necrosis factor (TNF) related protein (CTRP) super-family. CTRP3 is found to function as adipokines that display diverse biological activities in metabolic and cardiovascular diseases. Recent study demonstrated that CTRP3 was protective against pathological cardiac remodeling in mice. Nevertheless, the effect of CTRP3 on vascular remodeling remains undefined. Our present study aimed to explore the effects of adipokine CTRP3 on the activation of adventitial fibroblasts (AFs) induced by TGF-β1. Immunofluorescent staining, real-time PCR and Western blot were conducted to evaluate the expression of α-smooth muscle-actin (α-SMA) and collagen I. The expression of CTGF was evaluated by enzymelinked immunosorbent assay (ELISA), while the proliferation and migration of adventitial fibroblasts were detected by using cell counting kit-8 (CCK-8) assay and Transwell technique, respectively. Functional analysis showed that CTRP3 inhibited TGF-β1 inducing AFs phenotypic conversion, collagen synthesis, proliferation and migration. The secretion of CTGF was also inhibited by CTRP3. Our findings suggest that CTRP3 may be beneficial to the prevention of cardiovascular diseases and provide a promising therapeutic strategy to attenuate vascular remodeling.

Myofibroblast

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Abnormal octadeca-carbon fatty acids distribution in erythrocyte membrane phospholipids of patients with gastrointestinal tumor

Medicine (2017)

Shaohui Lin Tianyu Li Xifang Liu Shihu Wei Zequn Liu

Fatty acid (FA) composition is closely associated with tumorigenesis and neoplasm metastasis. This study was designed to investigate the differences of phospholipid FA (PLFA) composition in erythrocyte and platelet cell membranes in both gastrointestinal (GI) tumor patients and healthy controls. In this prospective study, 50 GI tumor patients and 33 healthy volunteers were recruited between the years 2013 and 2015. Blood samples were collected from healthy volunteers and patients, and FA composition was assessed using gas chromatography-mass spectrometer (GC-MS), and data were analyzed by multifactor regression analysis. Compared with healthy controls, the percentages of C18:0 (stearic acid, SA), C22:6 (docosahexaenoic acid, DHA), and n-3 polyunsaturated FAs (n-3 PUFA) were significantly increased, while C18:1 (oleic acid, OA), C18:2 (linoleic acid, LA), and monounsaturated FAs (MUFA) decreased in erythrocyte membranes of GI tumor patients. Also, patient's platelets revealed higher levels of C20:4 (arachidonic acid, AA) and DHA, and lower levels of OA and MUFA. Our study displayed a remarkable change in the FA composition of erythrocyte and platelet membranes in GI tumor patients as compared with healthy controls. The octadeca-carbon FAs (SA, OA, and LA) in erythrocyte membranes could serve as a potential indicator for GI tumor detection.

Stearic acid

10.1097/md.0000000000007189

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Evaluation of the Wound Healing Potential ofResina Draconis (Dracaena cochinchinensis)in Animal Models

Evidence-based Complementary and Alternative Medicine (2013)

Huihui Liu Shaohui Lin Dan Xiao Zheng Xiao Yan Gu

Resina Draconis (RD) is a type of dragon's blood resin obtained from Dracaena cochinchinensis (Lour.) S.C. Chen (Yunnan, China). It has been used as a medicine since ancient times by many cultures. The ethanolic extract of Resina Draconis (RDEE) was evaluated for its wound-healing activity using excision and incision wound models in rats. Group I, the control group, was treated with ointment base. Group II, which served as a reference standard, was treated with moist exposed burn ointment (MEBO). Group III was treated with RDEE. The parameters observed were percentage of wound contraction, epithelialization period, tensile strength, histopathological studies, microvessel density (MVD), and the expression of vascular endothelial growth factor (VEGF) and transforming growth factor- β 1 (TGF- β 1). The group treated with RDEE showed significantly better wound contraction and better skin-breaking strength as compared with the control group. The results of histopathological examination, MVD, and the expression levels of growth factors supported the outcome of the wound models as well. The present study provided a scientific rationale for the traditional use of RD in the management of wounds.

Microvessel

10.1155/2013/709865

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Citations (46)

Association between vitamin D deficiency and fragility fractures in Chinese elderly patients: a cross-sectional study

Annals of Palliative Medicine (2020)

Qinnan Wang Dongjuan Yu Jiajun Wang Shaohui Lin

Background: Vitamin D deficiency can lead to osteoporosis and increases the risk of fractures. Vitamin D deficiency is prevalent in Asian populations, however, research focusing on the association between vitamin D deficiency and osteoporotic hip fractures, particularly in Chinese populations is scarce. The aim of this study was to explore the relationship between vitamin D deficiency and osteoporotic hip fractures in elderly Chinese patients. Methods: This cross-sectional study recruited 60 patients (aged 65 years and over) who had hip fractures. Serum 25-hydroxyvitamin D (25OHD) levels were examined using an electrochemiluminescence immunoassay. The patients were classified into two groups based on their bone mineral density (BMD); subjects with osteoporosis (BMD t value ≤–2.5) and individuals without osteoporosis (BMD t value ≥–1.0). Serum 25OHD was further analyzed in these two groups. Results: Across all of the patients with hip fractures, approximately 10% showed vitamin D deficiency, and a further 53.33% showed vitamin D. insufficiency. Furthermore, of the patients with hip fractures, those with osteoporosis had significantly lower levels of serum 25OHD (25.43±6.35 ng/mL) compared to those patients without osteoporosis (30.70±7.17 ng/mL). The incidence of vitamin D insufficiency (66.67%) and deficiency (16.67%) was higher in the patients with osteoporosis than in those without osteoporosis (insufficiency 44.44% and deficiency 5.56%, respectively). Conclusions: Our study confirmed a high prevalence of vitamin D insufficiency in elderly patients with hip fractures in China. Our data suggests that vitamin D plays an important role in the occurrence of hip fractures in these elderly patients, and especially where patients also suffer from osteoporosis.

Hip Fracture

Cross-sectional study

10.21037/apm-19-610

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Citations (20)