Carla T. M Sayago

Universidade Federal do Pampa

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Andreas Sebastian Loureiro Mendez

Universidade Federal do Rio Grande do Sul

Fábio de Souza Barbosa

Universidade Federal do Rio Grande do Sul

Luciano Mantovani

Universidade Federal do Pampa

Cássia Virgínia Garcia

Universidade Federal do Rio Grande do Sul

Vanessa Brum de Camargo

Universidade Federal do Pampa

Elfrides Eva Scherman Schapoval

Universidade Federal do Rio Grande do Sul

Claudia Ortiz Alves Gularte

Universidade Federal do Pampa

Fávero Reisdorfer Paula

Universidade Federal do Pampa

Valdir Cechinel Filho

Universidade do Vale do Itajaí

Geovana Pereira

Universidade Federal do Pampa

Cooperative Institutions

Universidade Federal do Pampa

Universidade Federal do Rio Grande do Sul

Universidade Federal de Santa Maria

Instituto de Ciências Farmacêuticas

University of Rio Grande and Rio Grande Community College

Universidade Regional Integrada do Alto Uruguai e das Missões

Natura (Brazil)

Universidade Federal do Rio Grande

Universidade Federal de Ciências da Saúde de Porto Alegre

Universidade Federal de Pernambuco

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Characterization of the antibiotic doripenem using physicochemical methods: chromatography, spectrophotometry, spectroscopy and thermal analysis

Química Nova (2011)

Andreas Sebastian Loureiro Mendez Luciano Mantovani Fábio de Souza Barbosa Carla T. M Sayago Cássia Virgínia Garcia

Doripenem was characterized through physicochemical and spectroscopic techniques, as well as thermal analysis. TLC (Rf = 0.62) and HPLC (rt = 7.4 min) were found to be adequate to identify the drug. UV and infrared spectra showed similar profile between doripenem bulk and standard. The ¹H and 13C NMR analysis revealed chemical shifts that allowed identifying the drug. Thermal analysis demonstrated three steps with mass loss, at 128, 178 and 276 ºC. The work was successfully applied to qualitative analysis of doripenem, showing the reported methods can be used for physicochemical characterization of doripenem

Doripenem

Characterization

Quantitative Analysis

Qualitative analysis

10.1590/s0100-40422011000900024

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Citations (8)

Stability-indicating RP-HPLC method for analysis of the antibiotic doripenem in pharmaceutical formulation—comparison to UV spectrophotometry and microbiological assay

Acta Chromatographica (2012)

Luciano Mantovani Carla T. M Sayago Vanessa Brum de Camargo V. F. Silveira Cássia Virgínia Garcia

Summary A stability-indicating liquid chromatographic (LC) method with UV detection was developed for the determination of doripenem in the marketed formulation (Doribax® 500 mg, powder for injection). A forced degradation study was conducted according to available guidelines and main references. Thermal, oxidizing, acidic and basic stress conditions were assayed to show the stability-indicating power of the method. Chromatographic separation was achieved using an isocratic elution method in a reversed-phase system using a mobile phase prepared from phosphate buffer and acetonitrile. Extensive degradation was observed under thermal, oxidative and basic treatment, and the products formed were detected without interference in the analysis of doripenem. To verify the efficiency of chromatographic run, the system suitability was studied. The theoretical plates (N = 5498.3) and tailing factor (tf = 0.951) were constant during repeated injections. The retention time of doripenem was 7.35 min and the method was validated within the concentration range 5–50 μg mL−1 (r = 0.999). Adequate results were obtained that indicate repeatability (RSD % = 1.03–1.37), inter-day precision (RSD % = 0.51) and accuracy. In comparison to spectrophotometric and microbiological methods, statistical analysis showed no significant difference between the obtained results. The proposed method was successfully applied to doripenem quantification, showing it is applicable to determine the antibiotic in the presence of degradation products and also that is a reliable method for routine analysis.

Doripenem

Repeatability

Forced degradation

Quantitative Analysis

Degradation

10.1556/achrom.24.2012.3.3

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Citations (9)

Chemical composition andin vitroantioxidant activity of hydro-ethanolic extracts fromBauhinia forficatasubsp.pruinosaandB. variegata

Acta Biologica Hungarica (2013)

Carla T. M Sayago Vanessa Brum de Camargo Fábio de Souza Barbosa Claudia Ortiz Alves Gularte Geovana Pereira

Bauhinia

10.1556/abiol.64.2013.1.3

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Citations (20)